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http://dx.doi.org/10.13047/KJEE.2021.35.5.503

A Study on the Induction of Infertility of Largemouth Bass (Micropterus salmoides) by CRISPR/Cas9 System  

Park, Seung-Chul (National Park Research Institute)
Kim, Jong Hyun (Nature And People Co., Ltd.)
Lee, Yoon Jeong (Nature And People Co., Ltd.)
Publication Information
Korean Journal of Environment and Ecology / v.35, no.5, 2021 , pp. 503-524 More about this Journal
Abstract
A largemouth bass (Micropterus salmoides) is an ecosystem disturbance fish species at the highest rank in the aquatic ecosystem, causing a serious imbalance in freshwater ecosystems. Although various attempts have been made to eradicate and control largemouth bass, no effective measures were found. Therefore, it is necessary to find an approach to maximize the effective population reduction based on the unique characteristics of largemouth bass. This study used the transcriptome analysis to derive 182,887 unigene contigs and select 12 types of final target sequences for applying the CRISPR/Cas9 system in the genes of IZUMO1 and Zona pellucida sperm-binding protein, which are proteins involved in sperm-egg recognition. After synthesizing 12 types of sgRNA capable of recognizing each target sequence, 12 types of Cas9-sgRNA ribonucleoprotein (RNP) complexes to be used in subsequent studies were prepared. This study searched the protein-coding gene of sperm-egg through the Next Generation Sequencing (NGS) and edited genes through the CRISPR/Cas9 system to induce infertile individuals that produced reproductive cells but could not form fertilized eggs. Through such a series of processes, it successfully established a composition development process for largemouth bass. It is judged that this study contributed to securing the valuable basic data for follow-up studies to verify its effect for the management of ecological disturbances without affecting the habitat of other endemic species in the same water system with the largemouth bass.
Keywords
AQUATIC ECOSYSTEM; ECOSYSTEM DISTURBANCE SPECIES; REPRODUCTION; NEXT GENERATION SEQUENCING; GENE EDITING;
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