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Physiological Activity of Sarcodon aspratus Extracts (능이버섯(Sarcodon aspratus) 추출물의 생리활성)

  • 송재환;이현숙;황진국;한정환;노정근;금동혁;박기문
    • Food Science of Animal Resources
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    • v.23 no.2
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    • pp.172-179
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    • 2003
  • This study was carried out to find the preventive medical and therapeutic effects of Sarcodon asparatus on adult disease by employing several biological and biochemical assays. Nitrate scavenging ability(NSA) of Sarcodan asparatus extracts was displayed up to 99.9% at pH 1.2 in a dose-dependent manner. They also had 90.4% electron donating ability(EDA) at the concentration of 0.1 mg/mL. Extracts of Sarcodon asparatus were also able to function as a powerful antioxidant at all concentrations(0.01∼l.0 mg/mL). Furthermore, we observed that 1 mg/mL concentration of the extracts was more powerful than BHT, With respect to fibrolytic activity, Sarcodon asparatus showed 1,843.8 unit/g, which was higher than streptokinase(1,189 unit/g). The inhibitory effects of the extracts on angiotensin converting enzyme, measured by the normal and pretreatment methods, were 53 and 58%, respectively. We also performed cytotoxicity effect of Sarcodon asparatus extracts on a various cancer cell lines. The growth inhibitory effects of the extracts(5.0 mg/mL) on A549, HeLa, AGS, and SK-Hep-1 cells were 78.9, 55.3, 69.0, and 42.5 %, respectively. Interestingly, Sarcodon asparatusextracts induced mutation on Salmonella typhimurium TA98 and TA100 when Ames test was done.

Effect of Parity of Hanwoo Cow on Physico-Chemical, Sensory and Nutritional Characteristics of Sullungtang (한우 암소의 산차가 설렁탕의 이화학적, 관능적 및 영양적 특성에 미치는 영향)

  • 김진형;박범영;조수현;유영모;채현석;이종문;안종남;김학균;김용곤
    • Food Science of Animal Resources
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    • v.20 no.2
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    • pp.87-92
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    • 2000
  • This study was performed to provide scientific information for consumers and to increase the quality and consumption of Sullungtang prepared with bone from Hanwoo. The physico-chemical, sensory, nutritional properties of Sullungtang by different parity were investigated. The extracting extents of blood were significantly higher for heifer among Hanwoo cows with different parity(none, the second or the fourth) and for back bones among different bone portions (round, hind shank, arm, fore shank or back bone) (p〈0.05). For Sullungtang extracted from bones of heifer was higher in collagen contents than those extracted from cows with the second or the fourth parity. Sullungtang extracted from bones of heifer and cows with the second parity had significantly higher contents of condroitin and viscosity than those of cows with the fourth parity, however there was no significant difference between heifer and cows with the second parity. In color, L values were significantly high, and a and b values were significantly low for Sullungtang extracted from bones of heifer when compared to those extracted from the other cows with the second parity. The contents of total nitrogen, sodium, or calcium were higher for Sullungtang extracted from heifer than those from the cows with the second or the fourth parity. In amino acid compositions, the percentages of glycine were highest and proline and glutamic acid were followed fro all Sullungtang samples from Hanwoo. The results showed that the overall quality of Sullungtang significantly decreased as the parity increased for Hanwoo cows. The Sullungtang extracted from bones of heifer had the best sensory scores as well as nutritional quality when compared to those extracted from the cows with parity. Therefore, the labeling and price for cow bones should be differently evaluated by their parity and age in marketing system.

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Effect of Backfat Thickness on the Carcass Grade Factors and Carcass Price in Hanwoo Cows and Steers (등지방 두께가 한우 암소와 거세우의 도체 등급 요인 및 도체가격에 미치는 영향)

  • Lee, Jong-Moon;Choe, Ju-Hui;Park, Hyun-Kyung;Kim, Yuen-Ho;Park, Beom-Young;Kim, Kwan-Tae;Koh, Kyung-Chul;Seo, Sang-Chul;Hwang, Kyu-Seok
    • Food Science of Animal Resources
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    • v.31 no.2
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    • pp.280-289
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    • 2011
  • Analyses were conducted to estimate the effect of backfat thickness (BFT) on carcass grade factors and carcass price in Hanwoo cows and steers. Data on grade factors and prices were collected from 69,159 Hanwoo carcasses (n = 13,376 cows, n = 55,783 steers) abtained at the Nonghyup Seoul slaughter house in 2009. Carcass BFTs were divided into nine categories from ${\leq}$ 3 mm to ${\qeq}$ 25 mm. The average BFTs were 11.93 mm in cows and 12.57 mm in steers. An increase in the BFT resulted in an increase in carcass weight, loin-eye area, skeletal maturity, fat color, but decreases in the yield index and yield grade (from grade A to C) in cows and steers. Carcass unit price (Won/kg) improved if the carcass was sold with a BFT of 13-15 mm in cows and 10-12 mm in steers. Marbling score and quality grade increased significantly to 21 mm BFT but decreased from a BFT of 22 mm in cows and steers. BFT was significantly correlated with yield index (cow, r = $-0.97^{**}$; steer, r = $-0.96^{**}$), marbling score (cow, r = $0.20^{**}$; steer, r = $0.14^{**}$), and carcass price (cow, r = $0.03^{**}$; steer, r = $-0.11^{**}$). These results could be used as a fundamental dataset for further research to increase the productivity of Hanwoo carcasses.

Production of Conjugated Linoleic Acid by Lactobacillus acidophilus Isolated from Breast-Fed Infants (모유 섭취 신생아 유래 Lactobacillus acidophilus에 의한 Conjugated Linoleic Acid 생성)

  • Park, Jeong-Gyu;Song, Won-Ho;Hong, Sung-Moon;Kim, Cherl-Hyun
    • Food Science of Animal Resources
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    • v.28 no.5
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    • pp.580-586
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    • 2008
  • Conjugated linoleic acid (CLA) is a mixture of positional and geometric isomers of linoleic acid with conjugated double bonds. These conjugated dienes were found to be responsible for many biological properties related to health. The objective of this study was to evaluate the production of cis-9, trans-11 CLA by Lactobacillus acidophilus isolated from breast-fed infants. Nine different cultures were tested for their ability to produce cis-9, trans-11 CLA from free linoleic acid in MRS broth and 8% reconstituted skim milk medium supplemented with linoleic acid at $37^{\circ}C$ for 48 hr. cis-9, trans-11 CLA was not detected or detected in very small amount when cell pellets of strains grown in MRS broth and 8% reconstituted skim milk supplemented with linoleic acid of $200{\mu}g/mL$. However, free cis-9, trans-11 CLA was produced in both media. It appeared that 8% reconstituted skim milk produced more cis-9, trans-11 CLA than MRS broth. L. acidophilus NB 203 and NB 209 produced more cis-9, trans-11 CLA than other tested cultures. The inhibitory effects of supplemented linoleic acid on the growth of L. acidophilus NB 203 and NB 209 were not detected up to $3,000{\mu}g/mL$ linoleic acid addition during the growth at $37^{\circ}C$ for 48 h. The production of cis-9, trans-11 CLA by these two L. acidophilus strains increased in the logarithmic growth phase until 24 hr incubation. Under this experimental condition, the best yield of CLA isomers for L. acidophilus NB 203 and NB 209 could be obtained from medium supplemented with $500{\mu}g/mL$ linoleic acid at $37^{\circ}C$ after 24 hr of incubation. These results indicate that the use of lactic acid bacteria producing free CLA in fermented dairy products may have potential health or nutritional benefits.

Dynamic Characteristics of Water Column Properties based on the Behavior of Water Mass and Inorganic Nutrients in the Western Pacific Seamount Area (서태평양 해저산 해역에서 수괴와 무기영양염 거동에 기초한 동적 수층환경 특성)

  • Son, Juwon;Shin, Hong-Ryeol;Mo, Ahra;Son, Seung-Kyu;Moon, Jai-Woon;Kim, Kyeong-Hong
    • Journal of the Korean Society for Marine Environment & Energy
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    • v.18 no.3
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    • pp.143-156
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    • 2015
  • In order to understand the dynamic characteristics of water column environments in the Western Pacific seamount area (approximately $150.2^{\circ}E$, $20^{\circ}N$), we investigated the water mass and the behavior of water column parameters such as dissolved oxygen, inorganic nutrients (N, P), and chlorophyll-a. Physico-chemical properties of water column were obtained by CTD system at the nine stations which were selected along the east-west and south-north direction around the seamount (OSM14-2) in October 2014. From the temperature-salinity diagram, the main water masses were separated into North Pacific Tropical Water and Thermocline Water in the surface layer, North Pacific Intermediate Water in the intermediate layer, and North Pacific Deep Water in the bottom layer, respectively. Oxygen minimum zone (OMZ, mean $O_2$ $73.26{\mu}M$), known as dysoxic condition ($O_2<90{\mu}M$), was distributed in the depth range of 700~1,200 m throughout the study area. Inorganic nutrients typified by nitrite + nitrate and phosphate showed the lowest concentration in the surface mixed layer and then gradually increased downward with representing the maximum concentration in the OMZ, with lower N:P ratio (13.7), indicating that the nitrogen is regarded as limiting factor for primary production. Vertical distribution of water column parameters along the east-west and south-north station line around the seamount showed the effect of bottom water inflowing at around 500 m deep in the western and southern region, and concentrations of water column parameters in the bottom layer (below 2,500 m deep) of the western and southern region were differently distributed comparing to those of the other side regions (eastern and northern). The value of Excess N calculated from Redfield ratio (N:P=16:1) represented the negative value throughout the study area, which indicated the nitrogen sink dominant environments, and relative higher value of Excess N observed in the bottom layer of western and southern region. These observations suggest that the topographic features of a seamount influence the circulation of bottom current and its effects play a significant role in determining the behavior of water column environmental parameters.

CO2 Exchange in Kwangneung Broadleaf Deciduous Forest in a Hilly Terrain in the Summer of 2002 (2002년 여름철 경사진 광릉 낙엽 활엽수림에서의 이산화탄소 교환)

  • Choi, Tae-jin;Kim, Joon;Lim, Jong-Hwan
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.5 no.2
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    • pp.70-80
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    • 2003
  • We report the first direct measurement of $CO_2$ flux over Kwangneung broadleaf deciduous forest, one of the tower flux sites in KoFlux network. Eddy covariance system was installed on a 30 m tower along with other meteorological instruments from June to August in 2002. Although the study site was non-ideal (with valley-like terrain), turbulence characteristics from limited wind directions (i.e., 90$\pm$45$^{\circ}$) was not significantly different from those obtained at simple, homogeneous terrains with an ideal fetch. Despite very low rate of data retrieval, preliminary results from our analysis are encouraging and worthy of further investigation. Ignoring the role of advection terms, the averaged net ecosystem exchange (NEE) of $CO_2$ ranged from -1.2 to 0.7 mg m$^{-2}$ s$^{-1}$ from June to August in 2002. The effect of weak turbulence on nocturnal NEE was examined in terms of friction velocity (u*) along with the estimation of storage term. The effect of low uf u* NEE was obvious with a threshold value of about 0.2 m s$^{-1}$ . The contribution of storage term to nocturnal NEE was insignificant; suggesting that the $CO_2$ stored within the forest canopy at night was probably removed by the drainage flow along the hilly terrain. This could be also an artifact of uncertainty in calculations of storage term based on a single-level concentration. The hyperbolic light response curves explained >80% of variation in the observed NEE, indicating that $CO_2$ exchange at the site was notably light-dependent. Such a relationship can be used effectively in filling up the missing gaps in NEE data through the season. Finally, a simple scaling analysis based on a linear flow model suggested that advection might play a significant role in NEE evaluation at this site.

Investigation on Characteristics of Summertime Extreme Temperature Events Occurred in South Korea Using Self-Organizing Map (자기조직화지도(Self-Organizing Map)를 이용한 최근 우리나라 여름철 극한온도 특성 분류)

  • Lim, Won-Il;Seo, Kyong-Hwan
    • Atmosphere
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    • v.28 no.3
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    • pp.305-315
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    • 2018
  • This study investigates the characteristic spatial patterns and dynamic processes associated with the summertime extreme temperature events in South Korea during the last 20 years (1995~2014) using Self-Organizing Map (SOM). The classified SOM patterns commonly have high temperature and anticyclonic circulation anomalies over South Korea. The two major teleconnection patterns are identified: one is from the subtropical western North Pacific (WNP) affecting to the north and the other is from the North Atlantic (NA) affecting downstream region. The meridional teleconnection pattern is related to the forcing of positive sea surface temperature (SST) anomaly over the WNP. The northward propagating Rossby wave generates the East Asia-Pacific (EAP) pattern to form an anticyclonic circulation anomaly over South Korea. On the other hand, NA SST anomalies generate an eastward Rossby wave train across the Eurasian continent, leading to the development of an anticyclonic circulation anomaly over South Korea. The EAP pattern occurs more frequently in July and August, whereas the midlatitude teleconnection pattern associated with NA SST anomalies develops more frequently in early summer (June).

A Study on the Irrigation Water Pollution of the Gimhae Plain (김해평야(金海平野)의 관개수(灌漑水) 오염(汚染)에 관한 연구(硏究))

  • Ha, Ho-Sung;Heo, Jong-Soo
    • Korean Journal of Environmental Agriculture
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    • v.1 no.1
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    • pp.22-30
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    • 1982
  • Water quality of downstream of the Nagdong river, using for agricultural irrigation of the Gimhae plain, were observed. Water temperature, turbidity, residue, pH, BOD, COD, DO, hardness, chloride, sulphate, phosphate, inorganic nitrogenous compounds, sodium, general bacteria, E. coli and heavy metals of the water were investigated at Daejeo, Sikman, Bongrim, Noksan, Machal and Jangyou pumping stations in the Gimhae plain in May, July and October, 1981. The results obtained were as follows; 1) Average value of analyzed components of the water at all sampling sites were 7.8 pH, 6.3 ppm BOD, 6.5 ppm COD, 6.4 ppm DO, 231 ppm hardness, 582 ppm Cl-, 412 ppm $SO_4--$, 2.32 ppm $PO_4---$, 3.8 ppm $NH_4+,\;478\;ppm\;Na+$, 2964 No. /100 ml total coliform, 0.0040 ppm Cd, 0.0066 ppm Pb, respectively. 2) The most heavily polluted site of all investigated ones was Sikman. It seemed to be caused by the vast quantity of wastewater discharged from industrial district in Gimhae city. The next polluted sites were Bongrim, Daejeo and Noksan, and comparatively less polluted sites were Machal and Jangyou, judging from both appearance and physicochemical observation. 3) At Sikman, the most heavily polluted site, average value of components were 8.0 pH, 8.1 ppm BOD, 8.2 ppm COD. These values were close to the limit point of agricultural water quality standard of 8.0 ppm BOD (COD). 4) Any apparent variation was not observed by the sampling season in most components except DO and $NH_4+$. DO of October was higher than that of May or July but $NH_4+$ was low. 5) $NH_4+$ content was comparatively high in downstream of the Nagdong river of which water is used as the agricultural irrigation in the Gimhae plain. Therefore, fertilizer application on the farming land must make account of nitrogen content of the irrigation water 6) It was considered that chloride and sodium contents would not influence the crop cultivation in common season, but in dry season irrigation must be done carefully.

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Neutralization of Pyrophyllite Mine Wastes by the Lime Cake By-Product (부산석회를 이용한 납석광산 폐석의 중화처리)

  • Yoo, Kyung-Yoal;Cheong, Young-Wook;Ok, Yong-Sik;Yang, Jae-E.
    • Korean Journal of Environmental Agriculture
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    • v.24 no.3
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    • pp.215-221
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    • 2005
  • Numerous abandoned or closed mines are present in the steep mountain valleys in Korea due to the depression of the mining industry since the late 1980s. From the mines, enormous amounts of wastes were dumped on the slopes causing sedimentation and acid mine drainage to be discharged directly into streams causing detrimental effects on surrounding environment. Objective of this research was to evaluate the feasibility of the lime cake by-product from the soda ash production (Solvay process) to neutralize the pyrophyllite mine wastes, which have discharged the acid drainage to soil and stream in the watershed. The pH of mine wastes was strongly acidic at pH 3.67 containing over 16% of $Al_2O_3$ and 11% of $Fe_2O_3$. Whereas the lime cake by-product was strongly basic at pH 9.97 due to high contents of CaO, MgO and $CaCl_2$ as major components. Column experiments were conducted to test the neutralizing capacity of the lime cake by-product for the acidic pyrophyllite mine wastes. The column packed with the wastes (control) was treated with the lime cake by-product, calcium carbonate, the dressing soil or combination. The distilled water was eluted statically through the column and the leachate was collected for the chemical analyses. Treatments of the mine wastes with the lime cake by-product (or calcium carbonate) as mixtures increased pH of the leachate from $3.5{\sim}4.0\;to\;7{\sim}8$. Concentrations of Fe and Al in the leachate were also decreased below 1.0 mg $L^{-1}$. A Similar result was observed at the combined treatments of the mine waste, the lime by-product (or calcium carbonate) and the dressing soil. The results indicated that the lime cake by-product could sufficiently neutralize the acid drainage from the pyrophyllite mine wastes without dressing soils.

A Study on the Forest Vegetation of Odaesan National Park, Korea (오대산국립공원 삼림식생에 관한 연구)

  • Kim, Chang-Hwan;Oh, Jang-Geun;Lee, Nam-Sook;Choi, Young-Eun;Song, Myoung-Jun
    • Korean Journal of Ecology and Environment
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    • v.48 no.1
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    • pp.61-67
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    • 2015
  • This study, which was conducted from Apr. 2013 to Jan. 2014, was carried out as part of a project of making a more detailed ecological zoning map with 1/5,000 scale. The necessity of electronic vegetation map with large scale has arisen in order to make the best use of basic research findings on resource monitoring of National Parks and to enhance efficiency in National Park management. In order to improve accuracy and speed of vegetation research process, the data base for vegetation research was categorized into five groups, namely broad-leaved forest, coniferous forest, mixed forest, rock vegetation and miscellaneous one. And then a vegetation map for vegetation research was created for the research on the site. What is in the database for vegetation research and the vegetation map reflecting findings from vegetation research showed similar distribution rate for broad-leaved forest with 71.965% and 71.184%, respectively. The distribution rate of coniferous forest (16.010%, 15.747%), mixed forest (10.619%, 12.085%), and rock vegetation (0.015%, 0.002%) did not have much difference. In a detailed vegetation map reflecting vegetation research findings, the broad-leaved mountain forest was the most widely distributed with 60.096% based on the physiognomy classification. It was followed by mountain coniferous forest (16.332%), mountain valley forest (15.887%), and plantation forest (3.558%) As for vegetation conservation classification evaluated in the national park, grade I and grade II areas took up 200.44 km2, 61.80% and 108.80 km2, 33.55% respectively. The combined area of these two amounts to 95.35%, making this area the first grade area in ecological nature status. This means that this area is highly worth preserving its vegetation. The high rate of grade I area such as climax forests, unique vegetation, and subalpine vegetation seems to be attributable to diverse innate characteristics of Odaesan National Park, high altitude, low level of artificial disturbance, the subalpine zone formed on the ridge of the mountain top, and their vegetation formation, which reflects climatic and geological characteristics, despite continuous disturbance by mountain climbing.