• Korean Journal of Clinical Laboratory Science
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• v.51 no.1
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• pp.78-85
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• 2019

Membrane proteins are involved in many common diseases, including heart disease and cancer. In various disease states, such as cancer, abnormal signaling pathways that are related to the membrane proteins cause the cells to divide out of control and the expression of membrane proteins can be altered. Membrane proteins have the hydrophobic environment of a lipid bilayer, which makes an analysis of the membrane proteins notoriously difficult. Therefore, this study evaluated the efficacy of two different methods for optimal membrane protein extraction. High-speed centrifuge and reagent-based method with a -/+ filter aided sample preparation (FASP) were compared. As a result, the high-speed centrifuge method is quite effective in analyzing the mitochondrial inner membranes, while the reagent-based method is useful for endoplasmic reticulum membrane analysis. In addition, the function of the membrane proteins extracted from the two methods were analyzed using GeneGo software. GO processes showed that the endoplasmic reticulum-related responses had higher significance in the reagent-based method. An analysis of the process networks showed that one cluster in the high-speed centrifuge method and four clusters in the reagent-based method were visualized. In conclusion, the two methods are useful for the analysis of different subcellular membrane proteins, and are expected to assist in selecting the membrane protein extraction method by considering the target subcellular membrane proteins for study.

• Journal of Korean Library and Information Science Society
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• v.49 no.4
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• pp.353-371
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• 2018

This paper aims to analyze the bibliographic characteristics and the material value of the recently introduced manuscript YoungNamRooSiun(嶺南樓詩韻). YoungNamRooSiun(嶺南樓詩韻) is a collection of six buildings including the Yeongnamroo(嶺南樓) main hall floor, Reulpadang(凌波堂), Chimryugak(枕流閣), Gaksadonghun(客舍東軒), Dukminjung(德民亭), and Namsujung(攬秀亭). And poetry. The number of artists listed here is 412, and the total number of works is 570 including 11 prose and 559 rhyme. Through the text, YoungNamRoo(嶺南樓) was able to confirm that it was a space where official affairs such as observatory, master's office space, and hospitality reception were held. Based on this, an attempt should be made to take the reliability of the YoungNamRoo(嶺南樓) Ruling through the comparison of the original texts of the YoungNamRoo(嶺南樓) Ruling Prize and the collected writers.

• Journal of Wetlands Research
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• v.20 no.4
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• pp.322-329
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• 2018

In this study the community characteristics of benthic macroinvertebrates distributed in stream of Gaya Mountain were figured out and the correlation with the physicochemical water quality factors were calculated. During the study period, total 4 phyla, 6 classes, 13 orders, 36 families, 100 species of benthic macroinvertebrates were recorded. Ephemeroptera were the most dominant followed by Trichoptera, Plecoptera, Diptera, Non-insecta, Hemiptera, and Odonata. The overall dominant species was Cincticostella levanidovae by dominance rate in 17.72%, subdominant species was Hydatophylax nigrovittatus by dominance rate in 6.15%. The biotic indices showed the diversity index 5.17, richness index 12.44, dominant index 0.24 and evenness index 0.78, there is no absolutely dominant benthic macroinvertebrates in the stream of Gaya Mountain. We identified the correlation between the species number and individuals of main taxa and the physicochemical water quality factors. Ephemeroptera and Plecoptera showed a negative correlation with water temperature, but they represented a positive correlation with the dissolved oxygen. This results suggest that water temperature and dissolved oxygen are important physicochemical water quality factors affects the distribution of Ephemeroptera and Plecoptera, the indicators of clean water stream.

• Clean Technology
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• v.24 no.4
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• pp.280-286
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• 2018

In this study, the adsorption performance of vapor phase VOCs under dry conditions was evaluated by using two metal oxides, $TiO_2$ powder and $Al_2O_3$ powder. BET analysis and ammonia in-situ FT-IR analysis were used to analyze specific surface area and surface acid site. As a result, $TiO_2$ powder and $Al_2O_3$ powder had a specific surface area of $317.6m^2\;g^{-1}$ and $64m^2\;g^{-1}$, respectively. In the case of $TiO_2$ powder, many acid sites were observed on the surface. As a result of evaluating the vapor phase VOCs adsorption performance using two metal oxide powders, $TiO_2$ powder having a relatively large specific surface area and a large number of acid sites exhibited relatively good adsorption performance. In particular, it is considered that the specific surface area directly affects the adsorption performance, and further study on the effect of the acid site is required. Based on the $TiO_2$ exhibited excellent adsorption performance, it manufactured into various forms of honeycomb, hollow fiber and disc. As a result, the adsorption performance was lower than that of the powder, but it is advantageous in view of applicability. In addition, it was confirmed that the disc adsorbent having excellent thermal durability due to the characteristics of the manufacturing process stably maintains adsorption performance even at a high temperature desorption process several times.

• The Journal of the Korea Contents Association
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• v.19 no.2
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• pp.409-423
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• 2019

The purposes of this study was to confirm the current issues of workforce policy of dental auxiliary personnel in Korea. The literature review and data collection were conducted in terms of the auxiliary personnel supply, regional distribution, legal works, working environment. Despite the rapid increase in the number of dental hygienists, the number of active dental hygienists is less than half of the total, and the dental clinics still experience the shortage. Considering the dental hygienist's work, the available dental auxiliary personnel for the dental assistance is limited. The proportion of dental hygienists is concentrated in large cities, and the ratio of dental clinics composed of only dental hygienists is high. Thus, it is essential to look for a plan to solve the disparity. The criterion for judging the legality of the works is ambiguous because a large portion of dental hygienist's work is not specified in the Medical Technicians Act. The job life is also shortened, and occupational profession and satisfaction are lowered. Thus, job burnout is accelerating. The difference between the legal works and the practiced works of dental hygienist and the conflict between the personnel might be due to the lack of utilization plans for the dental personnel at the government level. Therefore, the government should establish the roles of dental hygienist in accordance with the demands of the dental health service, institutionalize the necessary policies accordingly, and make efforts to establish plans to improve the relevant laws and utilize the manpower appropriately.

• Journal of Life Science
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• v.28 no.12
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• pp.1477-1488
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• 2018

This study investigated the muscles, intestines, and gonads of Sulculus diversicolor supertexta to examine the diversity of microbial communities within examples collected from the Jeju Coast. Using different media, initial pure isolation in MA, 1% BHIA, and 1% TSA indicated that the muscles, intestines, and gonads supported more communities, respectively. In analysis of relative similarity with 16s rRNA sequencing, 190 pure colonies were isolated, and further analysis with NBLAST identified 71 species, 39 genera, 25 families, and five phyla. Homogeny with the reference strain was 91-100%. Microbial communities in S. supertexta consisted of gamma and alpha Proteobacteria (48%), Actinobacteria (32.5%), Firmicutes (16.9%), Deinococcus-Thermus (1.3%), and Bacteroides (1.3%). In all tissue, Psychrobacter cibarius in Moraxellaceae was dominant. Alteromonadaceae, Enterobacteriaceae, Pasturellaceae, Moraxellaceae, Rhodobacteraceae, Geminicoccaceae, Dietziaceae, Intrasporangiaceae, Microbacteriaceae, Micrococcaceae, Micromonosporaceae, Streptomycetaceae, Aerococcaceae, Bacillaceae, Paenibacillaceae, Planococcaceae, and Staphylcoccaceae were commonly isolated across all tissues, and Flavobacteriaceae, Corynebacteriaceae, Yesiniaceae, Vibrionaceae, Hahellaceae, Pseudomonadaceae were also identified from the intestines. In microbial monitoring of four harmful bacteria, Streptomyces albus (96%) showed antibacterial activity against all four strains, and Agrococcus baldri (99%) and Psychrobacter nivimaris (99%) presented against E. Coli and E. aerogens. In addition, some strains with low homogeny were isolated and further experiments are therefore required, for example to refine the antimicrobial substances including new strain investigations. These additional experiments would aim to establish generic resources for the microbial communities in S. Supertexta and provide basic data for applied microbiological research.

• Journal of Digital Convergence
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• v.16 no.12
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• pp.511-521
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• 2018

The purpose of this study was to investigate the effect of critical thinking, clinical decision making ability, patient safety knowledge and attitude ability to perform patient safety activities during clinical practice on nursing student's. Data were analyzed using t-tests and one-way ANOVA using the SPSS 21.0 program. As a result, the subjects' safety nursing activities was positively correlated critical thinking disposition(r=.278, p=.001), clinical decision making ability(r=.202, p=.014), patient safety attitude(r=.421, p<.001).The significant predictors of perform safety nursing activities were gender(${\beta}=.175$, p=.031), patient safety education(${\beta}=-2.266$, p=.025), critical thinking disposition(${\beta}=3.354$, p=.001), patient safety attitude(${\beta}=.368$, p<.001). These factors explained 31.5% of the variance(R2=.561, F=4.56 p<.001). It is necessary to establish a curriculum for critical thinking disposition, clinical decision making ability, and patient safety education in order to improve patient safety performance necessary for clinical practice education of nursing college students.

• Clean Technology
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• v.25 no.1
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• pp.1-6
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• 2019

Effects of the Cu and K addition and the reduction condition of Fe-based catalysts for Fischer-Tropsch reaction are studied in a continuous flow reactor in this research. The catalysts for the reaction were prepared by homogeneous precipitation followed by incipient wetness impregnation. Physicochemical properties of the $Al_2O_3$ supported Fe-based catalysts are characterized by various methods including X-ray diffraction (XRD), temperature programmed reduction (TPR), and scanning electron microscopy (SEM). Catalytic activities and stabilities of the Fe/Cu/K catalyst are investigated in time-on-stream for an extended reaction time over 216 h. It is found that a reduction of the catalysts using a mixture of CO and $H_2$ can promote their catalytic activities, attributed to the iron carbides formed on the catalysts surface by X-ray diffraction analysis. The addition of Cu induces a fast stabilization of the reaction reducing the time to reach at the steady state by enhancement of catalytic reduction. The addition of K to the catalysts increases the CO conversion, while the physical stability of catalyst decreases with potassium loading up to 5%. The Fe/Cu (5%)/K (1%) catalyst shows an enhanced long term stability for the Fischer-Tropsch reaction under the practical reaction condition, displaying about 15% decrease in the CO conversion after 120 h of the operation.

• Journal of the Korean Applied Science and Technology
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• v.36 no.2
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• pp.676-684
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• 2019

The Pictet-Spengler reactions have widely known for the organic synthesis or biosynthesis of biologically active compounds, tetrahydro-${\beta}$-carbolines. We have developed the simple and efficient synthetic method for the synthesis of ${\beta}$-carbolines in water. Their chemical structures were characterized by nmr and UPLC/MS/QTOF. Calculated masses of compound 1 ($C_{17}H_{17}N_2$ 249.1392), 2 ($C_{17}H_{23}N_2$ 255.1861), 3 ($C_{19}H_{21}N_2O_3$ 325.1552) and 4 ($C_{19}H_{19}N_2O$ 279.1497) were almost identical with the detected masses of compound 1 (249.1315), 2 (255.1789), 3 (325.1460) and 4 (279.1364) respectively. Those synthesized four compounds showed strong antibiotic activity against the common E. coli.

• Health Policy and Management
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• v.29 no.2
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• pp.206-219
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• 2019

This paper aims to demonstrate current health expenditure (CHE) and National Health Accounts of the years 2018 constructed according to the SHA2011, which is a manual for System of Health Accounts (SHA) that was published jointly by the Organization for Economic Cooperation and Development (OECD), Eurostat, and World Health Organization in 2011. Comparison is made with international trends by collecting and analyzing health accounts of OECD member countries. Particularly, scale and trends of the total CHE financing as well as public-private mix are parsed in depth. In the case of private financing, estimation of total expenditures for (revenues by) provider groups (HP) is made from both survey on the benefit coverage rate of National Health Insurance (by National Health Insurance Service) and Economic Census and Service Industry Census (by National Statistical Office); and other pieces of information from Korean Health Panel Study, etc. are supplementarily used to allocate those totals into functional classifications. CHE was 144.4 trillion won in 2018, which accounts for 8.1% of Korea's gross domestic product (GDP). It was a big increase of 12.8 trillion won, or 9.7%, from the previous year. GDP share of Korean CHE has already been close to the average of OECD member countries. Government and compulsory schemes' share (or public share), 59.8% of the CHE in 2018, is much lower than the OECD average of 73.6%. 'Transfers from government domestic revenue' share of total revenue of health financing was 16.9% in Korea, lower than the other social insurance countries. When it comes to 'compulsory contributory health financing schemes,' 'transfers from government domestic revenue' share of 13.5% was again much lower compared to Japan (43.0%) and Belgium (30.1%) with social insurance scheme.