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Phylogenetic characteristics of bacterial populations and isolation of aromatic compounds utilizing bacteria from humus layer of oak forest (상수리림 부식층으로부터 방향족 화합물 분해세균의 분리 및 세균군집의 계통학적 특성)

  • Han, Song-Ih
    • Korean Journal of Microbiology
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    • v.52 no.2
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    • pp.175-182
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    • 2016
  • In this study, we isolated aromatic compounds (lignin polymers) utilizing bacteria in humus layer of oak forest and investigated phylogenetic characteristics and correlation with major bacterial populations in the humus layer by pyrosequencing. Forty-two isolates using aromatic compounds such as p-anisic acid, benzoic acid, ferulic acid and p-coumaric acid were isolated and phylogentic analyses based on 16S rRNA gene sequences showed that the isolates belonged to the genus Rhizobium, Sphingomonas, Burkhorlderia, and Pseudomonas. Among these, Burkhorlderia species which belong to Betaproteobacteria class occupied 83% among the isolates. The bacterial populations in humus layer of oak forest were characterized by next generation pyrosequencing based on 16S rRNA gene sequences. The humus sample produced 7,862 reads, 1,821 OTUs and 6.76 variability index with 97% of significance level, respectively. Bacterial populations consist of 22 phyla and Betaproteobacteria were the major phylum consisting of 15 genera including Burkholderia, Polaromonas, Ralstoria, Zoogloea, and Variovorax. Approximately fifty percentage of them was Burkholderia. Burkholderia as the majority of population in the humus was considered to play a role in degrading lignin in humus layer of oak forest.

Scientific Analysis and Conservation Treatment of the Kettle Excavated from Hwangnam-dong, Gyeongju (경주(慶州) 황남동(皇南洞) 출토(出土) 철부(鐵釜)의 보존처리(保存處理) 및 과학적분석(科學的分析))

  • Moon, Sunyoung;Jeon, Ikhwan;Yu, Heisun
    • Conservation Science in Museum
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    • v.4
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    • pp.23-32
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    • 2003
  • As the kettle excavated from a site in Hwangnam-dong, Gyeongju was presumed to have been used as a melting crucible in a glass production workplace, we decided to prove its usage by scientific analysis. First, we performed conservation treatment to find the original status of the kettle, and then SEM-EDS and XRD analysis of the five corrosions created on the surface of the kettle. The fragment of the spout, which played a crucial role for the kettle to be considered as a melting crucible, was discovered during the conservation treatment. So the mouth rim of the kettle was restored to perfection. When we observed the microstructure of the metal sample, it was proved to be cast iron gradually cooled without heat treatment. In the corrosion products, the main components were Fe and O and other components such as P, Si, Ca, and S were found. The main compounds were quartz, vivianite, goetheite, akaganite, lepidocrocite, hematite, etc. Although these components were used as raw materials for making glass, these were found not in the melting status but mere raw materials. This can be an evidence to show these site where the kettle was excavated had been a glass production workplace. However, it is not sure that the kettle was used as a melting crucible. Moreover, if we consider the organic mold and sand clay accumulated in the well site when the kettle was excavated at first, we can see this as a formative factor of the corrosions of the kettle. Therefore, we concluded that the kettle is a typical cast iron and was not used as a melting crucible of glass.

Analysis of the reasons why single women in their thirties or forties choose not to marry: - implications for population education - (30-40대 싱글여성이 '결혼을 하지 않는 이유'분석 - 인구교육의 시사점 도출을 위하여 -)

  • Wang, Seok Soon;Jun, Joo Ram;Ryu, Kyung Hee
    • Journal of Korean Home Economics Education Association
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    • v.27 no.2
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    • pp.35-51
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    • 2015
  • The purpose of the present study is to investigate the various reasons that might lead single women to choose not to marry. Semi-structured in-depth interviews were arranged with 18 single women who met the criteria for the present research purpose. We considered only those responses of the interviewees that are directly related to their reasons why they choose not to marry, where the collected data were analyzed in three steps by methods of thematic analysis. As a result of the analysis, the reasons for which they chose not to marry could be grouped into three main clusters of themes which may be labeled as (1) themes centered on 'Myself', (2) themes centered on 'Family' and (3) themes centered on 'Surrounding Environments and Friends'. Among the first category of themes of 'Myself', we have found five sub-themes such as "Lack of emotional communication", "Not-found spouse meeting my criteria", "My personality", "Self-narcissism or self-centeredness", "False beliefs in marriage". As for the second category of themes 'Family', three sub-themes have been found including "Family of origin conflict", "Closeness to family of origin", "Comfortable daily lives". And for the final category of themes 'Surrounding Environments and Friends', there were found three sub-themes which include "Negative effects of married friends", "Emotional support system", "Changing social atmosphere". In all there are eleven sub-themes to consider. On the basis of these results, we presented some conclusions on the reasons why single women in their thirties or forties choose not to marry. We also presented some implications of these results on population education and future research.

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A study on the causes of the dislikes of elementary school students regarding Practical Arts classes (초등학교 실과수업 기피요인에 대한 연구)

  • Yu, Nan-Sook
    • Journal of Korean Home Economics Education Association
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    • v.24 no.2
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    • pp.1-15
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    • 2012
  • This study investigated the causes of the dislikes of Practical Arts education of elementary school students. This study was to provide the foundation for the proposition of alternative plans to make more students participate pleasantly and actively in Practical Arts classes. Data were collected via an open-ended survey from 273 elementary school students and through telephone interviews with five elementary school teachers. The data were analyzed inductively by NVivo. After the analysis of the student data, the analysis of the teacher data was conducted according to the variables from the student data. The results of this research were as follows: First, with regard to the causes of the students' dislikes of Practical Arts classes, extrinsic factors such as instruction management, instruction content, teaching facilities and equipment, and teacher characteristics were elicited. Intrinsic factors included complaints about failing to meet students' expectation from Practical Arts, negative perception of Practical Arts, the interest, competency, and fear of the students. Secondly, the students who learned Practical Arts from the Practical Arts specialty teachers gave more answers on instruction management, instruction content, and teacher characteristics than the students who learned Practical Arts from their homeroom teachers did. The students who learned Practical Arts from their homeroom teachers answered that they disliked Practical Arts because of lack of lab facilities and practice than their counterparts did. The analyses of teacher data provided the reasons and contexts of the dislikes of the students such as teaching facilities and equipment, and the anxiety of the students.

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Study on the safety management of radiation: centering on the radiation workers in medical institutions (방사선안전관리에 대한 조사 : 의료기관 방사선조사자를 중심으로)

  • 한은옥;문인옥
    • Korean Journal of Health Education and Promotion
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    • v.19 no.2
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    • pp.99-113
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    • 2002
  • While the use of radiation in the medical field provides diagnosis and treatment with important benefits, we cannot deny that the radiation bombing causes some hindrances. The expansion of radiation use in modem medicine is essential, so the radiation use and preparation of proper measure for safety management has risen as a pressing subject. Therefore, in order to make defensive plans for the prevention of health obstacles to general users of radiation and for the provision of basic data of the health education programs to radiation workers by grasping the knowledge, attitude and behavior towards the radiation safety management of radiation workers in each medical institution and by analyzing the factors that affect the actions of radiation safety management, in this study we conducted questionnaires from September 26 to November 5, 2001 targeting 805 radiation workers in 108 medical institutions including university hospitals, general hospitals, hospitals, clinics and public health centers etc. located in Seoul, four metropolitan cities and small and medium cities, and has obtained the following results. 1. The average point of knowledge on the radiation safety management was 10.96 out of 15. As for the general characteristics, the level of knowledge on radiation safety management was higher with older age, high education background and longer career. 2. The average point of attitude on the radiation safety management was 66.36 out of 75. The attitude point for general characteristics were higher with higher education background, longer career and in case of universities, the level of attitude on the radiation safety management was high. 3. The average of action points on the radiation safety management was 56.09 out of 75. In general characteristics, the action level of radiation safety level was higher with older age, longer career, and the reception of radiation education and in case of university hospitals. 4. It is analyzed that the relation of knowledge, attitude and behavior on the radiation safety management is higher as the levels of knowledge and attitude on the radiation safety management is higher. 5. As a result of analyzing the factors that affect the knowledge on the radiation safety management, the variables that can be explained best was in the order of ‘the behavior on the radiation safety management’, ‘work career’, ‘the attitude on the radiation safety management’, and ‘school career’. 6. As a result of analyzing the factors that affect the attitude on the radiation safety management, the variables that can be explained best was in the order of ‘the behavior on the radiation safety management’, ‘the knowledge on the radiation safety management’, and ‘school career’. 7. As a result of analyzing the factors that affect the behavior on the radiation safety management, the variables that can be explained best was in the order of ‘the attitude on the radiation safety management’, ‘the knowledge on the radiation safety management’, and ‘the frequency of radiation education

Characteristics of Submerged and Solid-State Fermentations for Production of Arachidonic Acid Mortierella alpina (Arachidonic Acid 생산을 위한 Mortierella alpina 곰팡이의 심부 및 고체 발효 특성 연구)

  • Shin Hyung Tai;Lee Soo Won;Park Ki Moon;Song Jae Whan;Suh Dong Sang;Lee Jae Heung
    • KSBB Journal
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    • v.20 no.1 s.90
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    • pp.60-65
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    • 2005
  • The objective of this work was to evaluate a solid-state fermentation process for the practical production of arachidonic acid(AA) by Mortierella alpina ATCC 32222. In the present investigation, batch culture kinetics for both submerged- and solid-state fermentations was carried out at $25^{\circ}C$ to identify the relationship between growth and arachidonic acid (AA) production. Glucose and yeast extract were used in submerged fermentations by using flasks, while rice bran was used as a sole raw material in the other type of fermentations by using a series of Petri dishes. It was evident that a mixed-growth associated pattern existed between the two variables, irrespective of modes of fermentations. The effect of carbon to nitrogen (CfN) ratio on AA production in solid-state fermentation was studied in the range of 6.5 - 20. As a result, an optimum condition was found to be 6.5. Supplementary carbon source was not necessary to meet the optimum C/N ratio. Unlike the Previous results obtained by other researchers, a supplement of sodium glutamate up to $4\%$ (w/w) to the rice bran medium did not have a positive effect on the AA productivity. However, an increase in AA productivity was obtained with the rice bran medium supplemented with sesame oil.

A Study on a Delivery System for the Effects of Using in the Public Libraries (공공도서관 활성화를 위한 Delivery System에 관한 연구 - 이동문고를 중심으로 -)

  • 이상근
    • Journal of the Korean BIBLIA Society for library and Information Science
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    • v.9 no.1
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    • pp.153-179
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    • 1998
  • This study attempt to suggest a policy for the effect of using in the public libraries by the verification that are different between groups by their using patterns and types of book collections. by using patterns and system of desired-book for user. by using patterns and system of desired-book for user. The questionnaire is distributed to the delivery lending library's user and librarian in public libraries. The summary of results are as follows 1 The Circulation period of public library is one month per every delivery lending library. 2. The Circulation volume of public library is '100-200' books per every times. 3. There are significant differences by their using patterns and types of book collections. 4. There are significant differences by their using patterns and system of desired-book for user. 5. There are significant differences by their using patterns and placement of book collections. Their results show that library is most effective place of book collection services. 6. Circulation services must carry out only in the sphere of jurisdiction 7. A Lost books must set up treatment basis of natural reduction ratio 8. The Interference factors of delivery lending library are lacks of human power, lost books and negative attitute of user in order 9. The Effects of using a delivery lending library are a positive attitute and specialty of top management, the optimum of budget and the effect of deposit system 10. Natural reduction ratio of lost books appropriates 0.5-0.6% of total loaned books

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An Analysis on Scientific and Technological Information Status and Demand of Small Businesses: Focuses on Busan·Ulsan·Gyeongsangnam-do Branches of KISTI (중소기업의 과학기술정보 요구 실태 분석 연구 - KISTI 부산·울산·경남지원을 중심으로 -)

  • Lee, Jong-Moon
    • Journal of the Korean BIBLIA Society for library and Information Science
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    • v.26 no.3
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    • pp.83-100
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    • 2015
  • This paper aims to survey the supply status and demand of the scientific & technological information among the 1,059 small enterprises demanding the support in Busan, Ulsan and Gyeongsangnam-do which have been supported by the Branch of KISTI and to propose the approaches to improve the scientific & technological information system. As the results of this research, Busan, Ulsan and Gyeongsangnam-do have very poor business and R&D environment to the extent that 96.3% of businesses was small business (medium business only for 3.7%) and 31% of total small & medium-cized businesses has less than 10 R&D workers. Small & medium-sized businesses which have demanded the information to KISTI account for only 0.2% in average for 3 years. The information demand types are market trend (26.8%), industrial property right (22.1%), business proposal (19.9%), item exploration (16.8%) and quality certification (8.4%). The supplied informations include scientific and technological data survey, market survey, translation and information application seminar. The business achievements from the information supply include 379 cases in technological development improvement and launch of products, 88 cases in technology certifications and 414 cases in patents and utility design application and registration. By the analysis results, the small and medium-sized businesses have difficulties in using R&D information by themselves. Thus, it's proposed to establish the science and technology information center in southeastern region and develop the information advisors specialized in local specialty industry categories. It's also proposed to expand the manpower and budget of KISTI concerning the information support for small & medium-sized businesses.

The Effect of Self Swallowing Exercise Program with Neuromuscular Electrical Stimulation(NMES) on Swallowing Function of Dysphagia (신경근 전기자극치료와 함께 적용된 자가 삼킴 운동 프로그램이 삼킴 장애 환자의 삼킴 기능 향상에 미치는 효과)

  • Yoon, In-Jin;Kim, Du-Ri;Cho, Young-Moon
    • The Journal of Korean society of community based occupational therapy
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    • v.5 no.1
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    • pp.23-34
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    • 2015
  • Objective : The purpose of this study is to investigate the effect of self swallowing exercise program with neuromuscular electrical stimulation(NMES) on swallowing function. Methods : Subjects who were diagnosed in dysphagia were randomly divided into the control group or experimental group. Both group were received NMES during 60 minutes with traditional swallowing therapy during 30 minutes. Additionally the experimental group was received self swallowing exercise during 30 minutes. We invested subject's characteristics through medical chart. We used VDS(Videofluoroscopic Dysphagia Scale) and PAS(Penetration Aspiration Scale) for assessing the swallowing function. Results : There were not significantly different in both group's pre swallowing function. The control group was significantly improved on pyriform sinus residue, aspiration, and VDS total score(p<.05). The experimental group was significantly improved on vallecular residue, pyriform sinus residue, and VDS total score(p<.05). Both group's difference of pre and post swallowing function were not significantly different. Conclusion : Self swallowing exercise and traditional swallowing therapy with NMES and traditional swallowing therapy with NMES are positive effect on swallowing function. The self swallowing exercise is not effective factor.

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Bioequivalence of Acer Capsule to Airtal Tablet (Aceclofenac 100 mg) (에어탈 정(아세클로페낙 100 mg)에 대한 에이서 캅셀의 생물학적 동등성)

  • Cho, Hea Young;Kim, Soo Jin;Oh, In Joon;Moon, Jai Dong;Lee, Yong Bok
    • Korean Journal of Clinical Pharmacy
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    • v.12 no.1
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    • pp.22-28
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    • 2002
  • Aceclofenac, 2-[(2',6'-dichlorphenyl)amino]phenylacetoxiacetic acid, is a new nonsteroidal anti-inflammatory drug that belongs to the family of phenylacetic acids. It shows good tolerance and potent analgesic/antiinflammatory properties, and acts on cartilaginous chondriocytes, stimulating their repair mechanism. The purpose of the present study was to evaluate the bioequivalence of two aceclofenac products, $Airtal^{TM}$ tablet (Daewoong Pharmaceutical Co.) and $Acer^{TM}$ capsule (Kyungdong Pharmaceutical Co.), according to the guideliner of Korea Food and Drug Administration (KFDA). The aceclofenac release from the two aceclofenac products in vitro was tested using KP VII Apparatus II method at pH 7.8 dissolution media. Sixteen normal male volunteers, $23.13\pm2.03$ years in age and $66.33\pm7.08$ kg in body weight, were divided into two groups and a randomized $2\times2$ cross-over study was employed. After one tablet or capsule containing 100 mg of aceclofenac was orally administered, blood was taken at predetermined time intervals and the concentrations of aceclofenac in serum were determined using HPLC with UV detector. The dissolution profiles of the two aceclofenac products were very similar at pH 7.8 dissolution media. The pharmacokinetic parameters such as $AUC_t,\;C_{max}\;and\;T_max$ were calculated and ANOVA test was utilized for the statistical analysis of the parameters. The results showed that the differences in $AUC_t,\;C_{max}\;and\;T_{max}$ between two products were $6.50\%,\;-1.06\%\;and\;11.96\%$ respectively, when calculated against the $Airtal^{TM}$ tablet. The powers $(1-\beta)\;for\;AUC_t,\;C_{max}\;were\;89.82\%\;and\;82.84\%$, respectively. Minimum detectable differences $(\Delta)\;at\;\alpha=0.05\;and\;1-\beta=0.8$ were less than $20\%\;(e.g.,\;17.51\%\;and\;19.30\%\;for\;AUC_t,\;C_{max}$, ). The $90\%$ confidence intervals were within $\pm20\%\;(e.g.,\;-3.73\%\sim16.73\%\;and\;-12.34\%\sim10.22\%\;for\;AUC_t,\;C_{max},\;respectively)$. Two parameters met the criteria of KFDA for bioequivalence, indicating that $Acer^{TM}$ capsule is bioequivalent to $Airtal^{TM}$ tablet.

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