• Journal of Life Science
• /
• v.15 no.2 s.69
• /
• pp.267-272
• /
• 2005

The objective of this study is to suggest the potentialities of nanoliposome composed of ceramide as an anti-irritant against various irritants used in cosmetics. Ceramides are major structural components of the epidermal permeability barrier, which is known to play an essential part in human physiology by not only preventing the loss of water from the body but also protecting the body from external physical, chemical, and microbial insults. According to the results, better effects on reinforcement of skin barrier function and anti-irritation were obtained with nanoliposome composed of ceramide than with dispersed ceramide. And, we performed in vitro skin penetration test using horizontal Franz diffusion cells with skin membrane prepared from hairless mouse to evaluate the influence of nanoliposome composed of ceramide on the skin penetration of lactic acid in formulations. From the results, we found that the anti-irritation effects of nanoliposome containing ceramide were due to reduced penetration rate of irritants. Conclusively, we could develop a new anti-irritation system and apply this nanoliposome composed of ceramide to the final cosmetic products successfully.

• Korean Chemical Engineering Research
• /
• v.48 no.6
• /
• pp.690-694
• /
• 2010

In this study, herbal wood vinegar including Bambusoideae, Cinnamomi Cortex, Zingiberis Rhizoma was tested to see possibility for cosmetic or skin related medicine. Anti-oxidation effect of herbal wood vinegar was tested by DPPH free radical scavenging activity, and showed 97% inhibition rate at $50{\mu}g/ml$. Anti-bacterial effect was tested by disc diffusion method, and it indicated strong anti-bacterial activity against normal skin flora Staphylococcus aureus. Whitening effect was measured by tyrosinase inhibition assay, and it was lower compared with vitamin C. Stability test was done by MTT assay, and cell toxicity was relatively high. Stability was also checked, and there was not significant change in color, aroma, appearance and pH during storage. Anti-atopic dermatitis test was done by hairless mouse and herbal wood vinegar recovered damaged skin to almost normal condition after 9 days of application. IgE concentration in herbal wood vinegar treated mouse was also reduced 30% compared with control. From the study, herbal wood vinegar showed good anti-oxidation, anti-bacterial and anti-atopic dermatitis effect, and had promising application in cosmetic or skin related medicine.

• Journal of the Society of Cosmetic Scientists of Korea
• /
• v.36 no.4
• /
• pp.271-280
• /
• 2010

Ultraviolet radiation (UV) is a major cause of photodamages to human skin and the immediate responses of the skin to UV include the erythema and edema. In an attempt to find effective UV-protecting agents to be used in cosmetics, a number of plant extracts were screened in the cell-based assays. Among the total of 38 plant extracts tested, 3 plant extracts derived from Sasa quelpaertensis, Althaea rosea, and Dryopteris crassirhizoma attenuated the UVB-induced cytotoxicity as well as melanin synthesis in cultured human epidermal melanocytes. The anti-inflammatory effects of these plant extracts were further examined in animal models. A control or test cream containing 1% of a plant extract was topically applied to ears of a C57BL/6 mouse or the dorsal skin of a SKH-1 hafirless mouse before and after the exposure to UVB. The change in ear thickness or dorsal skin redness due to UVB exposure was determined to monitor edema and erythema, respectively. All three test creams exhibited anti-inflammatory effects in both experiments. The creams containing Sasa quelpaertensis, Althaea rosea or Dryopteris crassirhizoma extract alleviated the UVB-induced edema response on day 4 by 53.8 %, 56.4 % and 31.1 %, respectively. They also inhibited the erythema formation on day 2 by 45.7 %, 34.1 % and 20.5 %, respectively. This study suggests that the selected plant extracts formulated in cosmetics may attenuate skin inflammation caused by overexposure to UV.

• YAKHAK HOEJI
• /
• v.60 no.1
• /
• pp.36-45
• /
• 2016

This study was aimed to enhance the percutaneous absorption of tizanidine hydrochloride (TZ) across Strat-M$^{TM}$ artificial membrane and excised hairless mouse skin using various vehicles and chemical permeation enhancers. Solubility studies were performed using hydrophilic and lipophilic vehicles. To initially evaluate vehicle effects on skin permeation, Strat-M$^{TM}$ membrane was adopted using Franz-type diffusion cells loaded with 0.4 mg donor dose. Effects of fatty acids on the permeation of TZ from PG and PGMC were compared, and the effects of various hydrophilic vehicles in the presence of linoleic acid were studied using excised hairless mouse skin specimens. The mean solubility (mg/ml) of TZ in hydrophilic vehicles was higher: water > PG > DMSO > ethanol > PEG 200 > NMP > PEG 300 > PEG 400 > DGME, and solubilities in lipophilic vehicles such as PGMC, PGMC, IPM, Captex 200 and Captex 300 were much less than 1.0 mg/ml. Permeation rates through StratTM membrane from pure vehicles were in the rank order: PGMC ${\geq}$ LBF > DMSO ${\geq}$ NMP ${\geq}$ PGML ${\geq}$ PG ${\geq}$ PEG 200 ${\geq}$ DGME ${\geq}$ EtOH. However, permeation rates of TZ through hairless mouse skin from pure vehicles were very low, although PG showed the highest flux ($1.66{\pm}0.28{\mu}g/cm^2{\cdot}hr$). Therefore, PG was selected in further studies. Addition of enhancers (3 v/v%) into PG markedly increased the flux (${\mu}g/cm^2{\cdot}hr$): oleyl alcohol ($14.9{\pm}3.1$) ${\geq}$ oleic acid ($14.5{\pm}1.6$) ${\geq}$ linoleic acid ($13.7{\pm}1.3$) > capric acid ($4.4{\pm}0.6$) > caprylic acid ($2.1{\pm}0.4$). Among hydrophilic vehicles with linoleic acid, PG and DMSO revealed relatively higher permeation for TZ. Increase of donor dose in PG resulted in dose-dependent permeation fluxes. These results suggest that permeation properties of TZ from nonaqueous solutions are markedly different between Strat-$M^{TM}$ membrane and excised hairless mouse skin, and transdermal delivery of TZ would be feasible with a combination of PG and enhancers.

• Magazine of the Korea Concrete Institute
• /
• v.11 no.4
• /
• pp.61-67
• /
• 1999

물론 이 글이 콘크리트 분야에 종사하는 사람이 알아야 하는 모든 것을 언급하지는 않는다 ; 본인의 저서 'Properties of Concrest'의 판매에 나쁜 영향을 주고 싶지도 않다. 내가 원하는 것은 콘크리트를 제작할 때 우리 모두가 주의를 기울여야 하는 중요한 몇 가지를 강조하는 것이다. 포트랜드 시멘트만 들어있는 보통의 콘크리트가 사용되는 시대는 이미 사라져가고 있고, 단순한 응용을 제외하고는 곧 사라질 것이다. 대부분의 경우는 가능한 일련의 시멘트계 재료 중에 선택을 해야 한다. 혼화제, 특히 고성능 감수제는 중요한 역할을 한다. 물을 많이 첨가하는 것이 작업성을 높이는 유일한 방법도 아니고 최선의 방법도 아니다. 성능을 기준으로 한 기준설정에 관여하는 사람은 이 모든 것을 명심해야 한다. 그렇지 않으면 시방기준에 명시된 규정과 근원적인 요구사항이 잘못 이해되고, 시험배합을 통해서만 잘못되었다는 것이 분명해지게 된다. 그 단계에서는 예상보다, 비록 틀린 것이지만, 더 많은 비용이 들 것이다. 시방규정은 통상적인 조항 외에 갈수록 내구성에 관심을 더 많이 보인다. 가끔은 내구성이 구조물의 기대수명에 대한 연수로 표현되기도 한다. 이 숫자를 배합재료에 관한 자료로 변환시키는 데는 여러 배합재료 및 배합비가 콘크리트의 성질에 미치는 영향에 관하여 깊이 알고 있어야 한다. 모든 사람이 그러한 구체적인 지식을 가질 필요는 없지만, 각 조직마다 상의할 수 있는 사람은 있어야 한다. 엔지니어가 언급한 규정이 따로 없고, 도면의 주를 참고하거나 구체적인 절이 명시되지 않은 상태에서 국가 시방기준을 통해서 조금씩 내용을 수집해야 하는 경우의 상황은 더 난감하다. 이러한 경우는 배합설계나 재료의 선택을 책임지고 있는 사람이, 환경에 대한 노출조건 및 필요한 배합재료에 관하여 잘 알고 있어야 한다. 일반적으로 말해서 콘크리트의 배합은 필요한 특정용도에 따라서 선택되어야 한다. 이것이 사람들이 고성능 콘크리트라 부르는 것이라고도 할 수 있다. 내 생각으로는 고성능 콘크리트와 보통 콘크리트의 경계는 곧 사라질 것이고, 앞으로 대다수 고객에게 주문 콘크리트 혹은 맞춤 콘크리트를 제공해야 할 것이다. 만약 이러한 접근방식에 더 많은 비용이 든다면, 그것을 보상하는 방법은 콘크리트에 대한 이미지를 높이고, 또한 앞으로 모든 용도에 계속적으로 이용될 수 있도록 하는 것이다. 아니면 구조물을 보수하고, 보수한 것을 또 보수하고, 해체하고 해야 할 것이다. 결국은 재활용 골재를 생산할 수 있다고 웃으면서 말할지 모르지만, 이런 식으로는 불만을 지닌 사용자가 콘크리트를 가능하면 멀리하게 만들 것이다. 콘크리트는 절대 심각한 경쟁에 처하지 않을 것이라는 가정은 무모하다. 내가 배합비에 대해서 강조해 왔지만, 이것으로 최종적으로 좋은 콘크리트가 된다는 보장은 없다. 비빔에서는 일반적으로 큰 문제는 없지만 골재에 함유된 수분을 더 잘 제어해야하고 주의를 게을리하지 말아야 한다. 비빔시간은 모든 재료가 잘 섞이기에 충분해야 하는데, 마이크로 실리카가 포함되어 있을 경우에는 더욱 그렇다. 단위시간당 믹서의 작업량을 높이기 위해서 충분히 비비지 않아서는 안된다. 그리고 마지막으로, 콘크리트가 타설되고 다져진 후, 적절하게 양생이 되어야 한다. 마이크로 실리카가 배합에 사용되었을 경우에는 특히 그러하지만, 이것은 모든 콘크리트에서 양생을 잘하는 것이 피복부에서 유해물질이 유입되는 것을 최소화하고 철근을 완전히 보호하는데 있어서 절대적이다. 내가 지금까지 말한 것은 콘크리트에 관한 충분한 지식과 이해를 필요로 한다. 이것은 당연하고 또한 적절한 것이라고 생각한다. 왜냐하면 더 이상 콘크리트를 수준낮은 싸구려 재료로 간주해서는 안되기 때문이다.

• Journal of Korean Society of Forest Science
• /
• v.88 no.1
• /
• pp.11-17
• /
• 1999

Morphological variations in leaves and leaf trichomes according to tree age were investigated for the identification of Quercus taxa at the different stages of development. The sizes of leaves from one-year-old seedlings of Q. serrata, Q. dentata, and Q. aliena were the smallest and increased with tree age. Blade length, blade width, petiole length, number of serration, and serration depth war also varied with tree age. Therefore, the examined taxa by the size and form of leaves were hardly identified at juvenile stages. The type, structure, and density of leaf trichomes of each species varied remarkably along with tree age. Three types of trichomes such as small stellate hairs, large stellate hairs, and long single hairs were observed on the abaxial side of leaves of Quercus taxa. In matured trees of Q. serrata both small stellate and long single hairs on leaves were observed. On the other hand large stellate hairs were found in leaves of Q. dentata. There were small stellate hairs in Q. aliens. In Q. mongolica var. crispula, no hair or small stellate hairs were occasionally found. It is possible to identify Q. serrata and Q. dentata by the characteristics of trichome even at two to three year old seedlings, while, it is difficult to tell Q. aliens and Q. mongolica var. crispula of seedlings from other taxa only by those of trichome.

• Journal of the Society of Cosmetic Scientists of Korea
• /
• v.34 no.2
• /
• pp.93-99
• /
• 2008

Until now, (-)-epigallocatechin-3-gallate(EGCG) is known as the most powerful antioxidant among green tea catechins having many beneficial effects on human skin. Considering that the content of catechins is variable according to many conditions such as solvent, temperature and pressure, we prepared the heat-epimerized-EGCG-mixture (HE-EGCG-mix) containing high content of gallocatechin-3-gallate(GCG) by epimerization during autoclaving process and found out its optimal condition for maximizing conversion from EGCG to GCG. To investigate the effects of EGCG and HE-EGCG-mix on skin barrier function, we performed in vivo experiments with hairless mice. We found that HE-EGCG-mix has more potent stimulating activity than EGCG for the production of involucrin 7(INV7) and for recovery of barrier function in SKH-1 mice. Also, we found that GCG stimulates $PPAR-{\alpha}$ transactivation more effectively than EGCG in vitro by transient transfection assay for $PPAR-{\alpha}$ activation activity. These imply that HE-EGCG-mix consisting of high content of GCG should stimulate more efficiently recovery of skin barrier through PPAR-mediated-kerationocyte differentiation than EGCG. In conclusion, our study may provide a possibility that GCG, the C-2 epimer of EGCG, could be a potentially effective agent for development of new cosmetics or health foods for recovery of skin barrier.

• Polymer(Korea)
• /
• v.36 no.4
• /
• pp.401-406
• /
• 2012

For bone regeneration from KUSA-A1 oesteoblast cells (KUSA), chitosan (CS) scaffolds possessing different surface properties, sponge-type (CSS) and nonwoven-type (CSNW), were manufactured. Surface area and pore size of CSNW were larger than those of CSS. On the other hand, the pore volume of CSNW was smaller than that of CSS. Cell attachment evaluation showed CSNW was more adequate then CSS, and this was attributed to the large surface area. For in vivo investigation, KUSA were seeded into CS scaffolds in wells followed by a week of cell culture. Obtained CS scaffolds with KUSA were implanted on the subcutaneous tissue of BALB/C nude mice. After surgery, implanted scaffolds were harvested and assayed by immunological staining. Network stability of CSS was better than that of CSNW, even if CSS scaffolds were destroyed between 4 and 6 weeks. Calcification was observed after 4 and 8 weeks for CSNW and CSS, respectively.

• Journal of the Korea Academia-Industrial cooperation Society
• /
• v.20 no.7
• /
• pp.203-210
• /
• 2019

A recent earthquake on the Korean Peninsula caused much damage to masonry buildings, and research on performance evaluation has been underway. A masonry building is generally constructed using wet construction and is affected by temperature, which reduces the efficiency of the construction. In this study, we propose a dry construction technique for assembling concrete blocks without using mortar and evaluated its performance through experimental and analytical research. To evaluate the performance, experiments were carried out for the prismatic compressive strength, direct terminal strength, and diagonal tensile strength of the dry construction wall. The adequacy of the cross section shape was also reviewed through FEM analysis. The results show that the compressive strength and diagonal tensile strength could exert a certain intensity or higher. Furthermore, the H-type module of a key block acted as a shear key for the entire concrete block, which resulted in excellent shear strength performance. In addition, the shape and thickness of the main block have a major effect on the strength performance of each block. Therefore, an optimal shape and the proposed dry construction method could be applied to replace the wet method by studying the construction or seismic performance of the proposed method.

• Journal of radiological science and technology
• /
• v.34 no.3
• /
• pp.239-244
• /
• 2011

In this study, we evaluated diffusion weighted imaging (DWI) to investigate whether the DWI parameters can predict characteristic parameters on pathologic specimens of tumor or not. CFPAC-1 was injected subcutaneously on the back flank of athymic nude mice (n=13) then two tumors were initiated on each mouse (2${\times}$13=26 tumors). The mice were sacrificed to make specimen immediately after initial MR imaging then were compared with the MR image. A dedicated high-field (7T) small-animal MR scanner was used for image acquisitions. A T1 and T2 weighted axial image using RARE technique was acquired to measure the T2 values and tumor size. DWI MR was performed for calculating ADC values. To evaluate tumor cellularity and determine the levels of MVD, tumor cells were excised and processed for H-E staining and immunostaining using CD31. T2 values and ADC values were computed and analyzed for each half of the tumors and compared to the correlated specimens slide. Median ADC within each half of mass was compared to the cellularity and MVD in the correlated area of pathologic slide. The mean of ADC value is $0.7327{\times}10^{-3}$ $mm^2/s$ and standard deviation is $0.1075{\times}10^{-3}$ $mm^2/s$. There is a linear relationship between ADC value and tumor necrosis (R2=0.697, p< 0.001). DW image parameters including the ADC values can be utilized as surrogate markers to assess intratumoral neoangiogenesis and change of the internal structure of tumor cells.