Browse > Article

Effect of Heat-epimerized-catechin-mixture Rich in Gallocatechin-3-gallate on Skin Barrier Recovery  

Kim, Jeong-Kee (Bioscience Research, Amorepacific Corporation R&D center)
Shin, Hyun-Jung (Bioscience Research, Amorepacific Corporation R&D center)
Lee, Sang-Min (Bioscience Research, Amorepacific Corporation R&D center)
Jeon, Hee-Young (Bioscience Research, Amorepacific Corporation R&D center)
Lee, Sang-Jun (Bioscience Research, Amorepacific Corporation R&D center)
Lee, Byeong-Gon (Bioscience Research, Amorepacific Corporation R&D center)
Publication Information
Journal of the Society of Cosmetic Scientists of Korea / v.34, no.2, 2008 , pp. 93-99 More about this Journal
Abstract
Until now, (-)-epigallocatechin-3-gallate(EGCG) is known as the most powerful antioxidant among green tea catechins having many beneficial effects on human skin. Considering that the content of catechins is variable according to many conditions such as solvent, temperature and pressure, we prepared the heat-epimerized-EGCG-mixture (HE-EGCG-mix) containing high content of gallocatechin-3-gallate(GCG) by epimerization during autoclaving process and found out its optimal condition for maximizing conversion from EGCG to GCG. To investigate the effects of EGCG and HE-EGCG-mix on skin barrier function, we performed in vivo experiments with hairless mice. We found that HE-EGCG-mix has more potent stimulating activity than EGCG for the production of involucrin 7(INV7) and for recovery of barrier function in SKH-1 mice. Also, we found that GCG stimulates $PPAR-{\alpha}$ transactivation more effectively than EGCG in vitro by transient transfection assay for $PPAR-{\alpha}$ activation activity. These imply that HE-EGCG-mix consisting of high content of GCG should stimulate more efficiently recovery of skin barrier through PPAR-mediated-kerationocyte differentiation than EGCG. In conclusion, our study may provide a possibility that GCG, the C-2 epimer of EGCG, could be a potentially effective agent for development of new cosmetics or health foods for recovery of skin barrier.
Keywords
epimerization; gallocatechin-3-gallate; involucrin; skin barrier function$PPAR-{\alpha}$;
Citations & Related Records
연도 인용수 순위
  • Reference
1 H. K. Lee, G. W. Nam, S. H. Kim, and S. H. Lee, Phytocomponents of triterpenoids, oleanolic acid and ursolic acid, regulated differently the processing of epidermal keratinocytes via PPAR-$\alpha$ pathway, Exp. Dermatol., 15, 66 (2006)   DOI   ScienceOn
2 C. Rodriquez-Caso, D. Agudo, F. Jimenez, and M. A. Median, Green tea epigallocatechin-3-gallate is an inhibitior of mammalian histidine decarboxyalse, Cell. Mol. Life Sci., 60, 1760 (2003)   DOI
3 S. H. Kim, G. W. Nam, H. K. Lee, S. J. Moon, and I. S Chang, The effects of Musk T on peroxisome proliferator-activated receptor [PPAR]-$\alpha$ activation, epidermal skin homeostasis and dermal hyaluronic acid synthesis, Arch. Dermatol. Res., 298, 273 (2006)   DOI   ScienceOn
4 R. Kumar, U. Ramachandran, S. Raichur, R. Chakrabarti, and R. Jain, Synthesis and evaluation of N-acetyl-L-tyrosine based compounds as PPAR-alpha selective activators, Eur. J. Med. Chem., 42, 503 (2007)   DOI   ScienceOn
5 Z. Y. Chen, Q. Y. Zhu, D. Tsang, and Y. Huang, Degradation of green tea catechins in tea drinks, J. Agric. Food. Chem., 49, 477 (2001)   DOI   ScienceOn
6 I. Ikeda, M. Kobayashi, T. Hamada, H. Goto, K. Imaizumi, and A. Nozawa, Heat-epimerized tea catechins rich in gallocatechin gallate and catechin gallate are more effective to inhibit cholesterol absorption than tea catechins rich in epigallocatechin gallate and epicatechin gallate, J. Agric. Food. Chem., 51, 7303 (2003)   DOI   ScienceOn
7 M. Dell'Agli, S. Bellosta, L. Rizzi, G. V. Galli, M. Canavesi, and F. Rota, A structure-activity study for the inhibition of metalloproteinase-9 activity and gene expression by analogues of gallocatechin- 3-gallate, Cell. Mol. Life Sci., 62, 2896 (2005)   DOI
8 S. Hsu, Green tea and the skin, J. Am. Acad. Dermatol., 52, 1049 (2005)   DOI   ScienceOn
9 P. M. Elias and E. H. Choi, Interactions among stratum corneum defensive functions, Exp. Dermatol., 14, 719 (2005)   DOI   ScienceOn
10 A. H. Conney, Y. P. Lu, Y. R. Lou, and M. T. Huang, Inhibitory effects of tea and caffeine on UV-induced carcinogenesis: relationship to enhanced apoptosis and decreased tissue fat, Eur. J. Cancer Prev., 11 (2002)
11 S. Hsu, T. Yamamoto, J. Broke, D. S. Walsh, and B. Singh, Green tea polyphenol-induced epithelial cell terminal differentiation is associated with coordinated expression of p57/KIP2 and caspase 14, J. Pharmacol. Exp. Ther., 312, 884 (2005)   DOI   ScienceOn
12 K. Yoshino, K. Ogawa, T. Miyase, and M. Sano, Inhibitory effects of the C-2 epimeric isomers of tea catechins on mouse Type IV allergy, J. Agric. Food. Chem., 52, 4660 (2004)   DOI   ScienceOn
13 Z. Y. Wang, M. T. Huang, T. Ferraro, C. Q. Wong, Y. R. Lou, and K. Reuhl, Inhibitory effect of green tea in the drinking water on tumorigenesis by ultraviolet light and 12-O-tetradecanoyl-phorbol-13- acetate in the skin of SKH-1 mice, Cancer Res., 52, 1162 (1992)   PUBMED
14 J. H. Lee, J. H. Chung, and K. H. Cho, The effect of epigallocatechin-3-gallate on extracellular matrix metabolism, J. Dermatol. Sci., 40, 195 (2005)   DOI   ScienceOn
15 S. M. Thacher and R. H. Rice, Keratinocyte-specific transglutaminase of cultured human epidermal cell: relation to cross-linked envelope formation and terminal differentiation, Cell, 40, 685 (1985)   DOI   ScienceOn
16 S. Balanehru and B. Nagarajan, Protective effect of oleanolic acid and ursolic acid against lipid peroxidation, Biochem. Int., 24, 981 (1991)   PUBMED
17 N. Mahindroo, Y. H. Peng, C. H. Lin, E. Prakash, T. W. Lien, and I. L. Lu, Structural basis for the structure-activity relationships of on peroxisome proliferator-activated receptor agonists, J. Med. Chem., 49, 6421 (2006)   DOI   ScienceOn
18 S. Balasubramanian, M. T. Sturniolo, G .R. Dubyak, and R. L. Eckert, Human epidermal keratinocytes undergo (-)-epigallocatechin-3-gallate-dependent differentiation but not apoptosis, Carcinogenesis, 26, 1100 (2005)   DOI   ScienceOn
19 K. Hanley, Y. Jiang, and S. He, Keratinocyte differentiation is stimulated by activators of the nuclear hormone receptor PPAR-$\alpha$, J. Invest. Dermatol., 110, 368 (1998)   DOI   ScienceOn
20 S. Sang, M. J. Lee, Z. Hou, C. T. Ho, and C. S. Yang, Stability of tea polyphenol (-)-epigallocatechin- 3-gallate and formation of dimmers and epimers under common experimental conditions, J. Agric. Food. Chem., 53, 9478 (2005)   DOI   ScienceOn
21 K. Dvorakova, R. T. Dorr, S. Valcic, B. Timmermann, and D. S. Alberts, Pharmacokinetics of the green tea derivative, EGCG, by the topical route of administration in mouse and human skin, Cancer Chemother. Pharmacol., 43, 331 (1999)   DOI