• Journal of The Korea Institute of Healthcare Architecture
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• v.12 no.3
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• pp.35-41
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• 2006

At the moment, a lot of interest in the research on Gnotobiotic Pigs are increasing in order to produce alternative human organs. So, it is very important to design and build proper housing facilities for Germ Free Pigs. Among the design issues related to Gnotobiotic Pigs' farms, circulation system takes a high position because it carries an important role in keeping the pig's housing environment aseptic. Considering those, this study aims to propose the guidelines for the design of circulation system in Germ Free Pig's facilities. The results of this study are as follows. At first, functional areas of Germ Free Pigs' facilities have been divided into three categories according to the clean level; aseptic area, semi-aseptic area, and non-aseptic area. Secondly, the basic principles of circulation system have been proposed. Finally, circulation system of Gnotobiotic Pigs' facility has been explored as a form of diagram according to the circulating subjects. These include human circulation, pig's circulation, and goods' circulation. This study has some limitations in that it is transcendent and lacks empirical evidence. Despite of some weaknesses, it is expected to give some useful guidelines for the design of circulation system in Germ Free Pigs' facilities.

• Current Research on Agriculture and Life Sciences
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• v.15
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• pp.47-52
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• 1997

The experiment was carried out to find the optimal condition for seed germination in vitro of Calanthe species. Seeds of Calanthe spp. after 90 days self pollination were well germinated in the MS medium. Germination condition of cross seeds between C. discolor and C. striata was much improved by pretreatment of micro sonic waves for 10 min. The days of germination was more shortened. and protocorm growth was more promoted in microsonic wave treatment than in non-treatment.

• Korean Journal of Environment and Ecology
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• v.14 no.3
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• pp.205-211
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• 2000

본연구는 조경소재로 이용가능성이 크지만 현재 자생지가 파괴되어 복원이 요구되고 있는 야생자란의 대량번식을 위해 무균배양시 배지 내 담함량의 변화와 펩톤, 트립톤 등의 첨가가 종자발아와 계대배양 후 유식물의 생육에 미치는 영향을 알아보고자 실시하였다. 배지 내 펩톤과 트립톤의 첨가는 발아에 영향을 주지는 않았지만, 당의 함량은 그 농도가 10g/L까지 증가함에 따라 발아율을 높였다. 또한 발아 후 유식물의 생육시 당의 첨가는 뿌리의 생육을 두드러지게 향상시켰으며, 생체중도 거의 2~3배정도 많았다. 하이포넥스 배지(대조구)에서는 높은 발아율을 보였지만 유식물의 생육은 트립톤 첨가배지(2g/L)에서 많았는데 엽수, 뿌리수, 엽장 근장, 생체중 등이 모두 다른 처리구의 2~3배에 이르는 초기생육을 보였다. 계대배양 이후의 생육상은 펩톤 첨가배지에서 가장 많은 생육량을 보였는데 특히 엽장과 엽폭 그리고 근장이 다른 처리구보다 월등히 높은 경향을 나타냈다. 생체중도 한 개체당 0.18g으로 가장 높게 나타나 펩톤의 첨가가 계대배양 이후의 생육을 크게 촉지시키는 것으로 나타났다. 결론적으로 하이포넥스 배지에 트립톤 2g/L를 첨가하였을 때 발아율과 유식물의 생육이 다른 배지에 대해 매우 양호한 것으로 나타나 자생 자란의 종자발아용 배지로 가장 적당한 것으로 사료된다. 그리고 이후 계대배양시에는 펩톤의 첨가 (3g/L)가 유식물의 생육을 가장 촉진시키는 것으로 나타났다.

• Korean Journal of Plant Tissue Culture
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• v.21 no.3
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• pp.183-186
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• 1994

Somatic embryos derived from cell suspension cultures of rice were singly alginate-encapsulated to be used as artificial seeds. When placed on half strength MS solid medium,73% of the encapsulated somatic embryos were capable of germination Encapsulation per se did not affect the germination frequency of embryos. When incubated by wrapping with moistured non-sterile filter paper, 60% of the encapsulated somatic embryos germinated. However encapsulated zygotic embryos without endosperm showed a high germination frequency regardless of the sterility of the incubation conditions. The results suggest that a greater susceptibility of somatic embryos to contaminants is attributed to lower germination frequency of encapsulated somatic embryos in non-sterile conditions.

• Tuberculosis and Respiratory Diseases
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• v.53 no.3
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• pp.332-336
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• 2002

Bacterical tracheitis is a very rare complication in adults after endotracheal intubation. We report a case of bacterial tracheitis associated with endotracheal intubation and corticosteroids. The patient was discharged with a permanent tracheostomy, and a resection and an end-to-end anastomosis of the trachea is planned.

• Journal of The Korea Institute of Healthcare Architecture
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• v.14 no.4
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• pp.39-46
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• 2008

Construction and operation of Germ Free Pigs' facilities are very expensive because pigs' rooms and other major rooms of the facility require germfree environments. Especially, running the HVAC system of aseptic facilities requires a lot of expenses. However, proper location and efficient shape of outlets/inlets for the ventilation of the room can reduce the excessive running cost. In order to do that, this study proposes alternative location and shape of ventilation outlets/inlets to the existing design pattern in germfree pigs' room. The design condition of this study is the maintenance of adequate temperature(24$^{\circ}C$, $NH_3$concentration level(below 1.5 ppm), and air stream speed(below .25m/sec) in the pigs' room for the summer and the winter together. As the Software Program, FLUENT(Ver. 6.2) has been used for the analysis of proposed ventilation patterns. In conclusion, wall inlets and ceiling inlet/outlet are advisable in summer, wall inlets and ceiling outlets is advisable in winter. As far as the shape is concerned, diffuser type for the ceiling outlet is desirable.

• Journal of Sericultural and Entomological Science
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• v.14 no.2
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• pp.67-72
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• 1972

It was first succeded in rearing the silkworm on the artificial diets in Japan in 1960. Since then, the researches on the artificial diets have been carried out intensively but the artificial diets have not been applied to the silkworm rearing practically till now. There are difficulties in rearing the silkworm on the artificial diets, such as uneven silkworm growth, higher production cost and decay. The results were not satisfactory, but the author hopefully expects that the artificial diet rearing would be possible for maintaining normal silkworm growth throughout improvement of diet composition, rearing techniques and aseptics add to the diets. Especially the author considered that the aseptic rearing will contribute to the research of sericultural science. Within a few years, the artificial diet rearing would be applied to the practical silkworm rearing.

• The Journal of the Korea institute of electronic communication sciences
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• v.16 no.3
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• pp.559-564
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• 2021

With the development of ICT, research on smart farms is steadily progressing in the agricultural field for the modernization of cultivation facilities. However, most of the current smart farms are not specific crops, but general-purpose systems that can be used in various fields. In this paper, an environmental control device and an integrated control system capable of creating a aseptic growing environment required for mushroom cultivation were proposed, and the system was designed, manufactured, and programmed. Through this, it is possible to build a smart farm optimized for crops that is needed to maintain a precise growing environment.

• Journal of East-West Nursing Research
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• v.15 no.1
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• pp.18-25
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• 2009

Purpose: The study was investigated to identify the relationship between nursing workload and aseptic technique performance by clinical nurses, and to decrease the incidence rate of nosocomial infection. Methods: Participants (N=283) were recruited in B city from April to June 2007. The data were collected by a structured questionnaire and analyzed with descriptive statistics, t-test, ANOVA and Pearson's correlation coefficient. Results: Nursing workload was rated 9.85 out of a total score of 15. The level of aseptic technique performance as the basis for insertion of a Foley catheter was 42.72 out of a total score of 50, and as basis for insertion of intravenous catheter for fluid therapy was 40.11 out of a total score of 55. There was not a significant relationship between aseptic technique performance and nursing workload. There was a significant positive relationship between the aseptic technique performance in insertion of Foley catheter and that of intravenous catheter for fluid therapy (r=.279, p<.001). Conclusions: Attention to asepsis by nurses is crucial in nosocomial infection-related clinical nursing skills.

• Clinical and Experimental Pediatrics
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• v.49 no.7
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• pp.745-750
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• 2006

Purpose : Enteroviruses are the most common cause of aseptic meningitis in patients of all ages. A definite diagnosis of enteroviral meningitis can be established by detection of virus directly in CSF specimens. But this is time-consuming and lacks sensitivity, so polymerase chain reaction(PCR) detecting of viral RNA in patient specimens such as CSF, stool has been demonstrated. But little is known about the influence of sampling time on the results of CSF PCR and stool PCR. We investigated diagnostic utility of PCR of CSF and stool according to sampling time after the onset of symptoms. Methods : PCR results were analyzed according to sampling time for 42 patients diagnosed aseptic meningits in our hospital from $11^{th}$ January to $30^{th}$ August, 2005. Results : The diagnostic yield of the test was higher of CSF specimens obtained ${\leq_-}2$ days after clinical onset(positive PCR results 9/18, 50 percent), compared with CSF collected >2 days after onset(positive PCR results 1/24, 4.2 percent)(P=0.001). Instead, positive PCR results of fecal specimens maintained highly(average 90.5 percent), 10 cases had also positive PCR results even 5-6 days after onset. 10 cases of CSF specimens had positive enterovirus PCR results containing coxsackievirus B5 (n=6), coxsackievirus B3(n=3). 38 cases of stool specimens had positive enterovirus PCR results containing echovirus 18(n=7), echovirus 9(n=3), coxsackievirus B5(n=8), coxsackievirus B3(n=3). 6 cases(coxackie B5) had positive CSF PCR and stool PCR, both. Conclusion : Stool PCR was clinically sensitive for detecting enterovirus during enteroviral meningits and could give a presumptive diagnosis throughout the disease course. A definite diagnosis was obtained by CSF PCR, but its utility was clearly lower for samples obtained >2 days after clinical onset. Therefore, it is recommended that, in addition to performance of CSF PCR, fecal samples obtained from patients with suspected enteroviral meningitis should be tested by PCR, especially when the duration of symptoms is >2 days.