• Title/Summary/Keyword: 무균

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The Cognition and Practice of Infection Control in Dental Workplace (근무지 유형에 따른 치과감염관리 인지도 및 실천도 조사)

  • Lee, YunHui;Choi, SungMi
    • Journal of the Korean Society of Radiology
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    • v.9 no.6
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    • pp.409-416
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    • 2015
  • This study is to examine the infection management cognition and to practice of dental hygienists about their general characteristics, type of workplace. 354 dental hygienists in Daegu and Kyungbuk cities. The questionnaire survey was performed from April to May, 2015. These showed that factors of dental infection management according to workplace depended existence and nonexistence that chief of station and program of infection management, education of infection control(p<.001). Also cognition of dental infection control according to workplace drawed the protection individual(p<.05), asepsis procedure(p<.01), control of dental equipment(p<.001), control of infectious waste(p<.01) and laundry(p<.001), to practice of dental infection control according to workplace drawed the protection individual and asepsis procedure(p<.001), methods of disinfected and sterilized(p<.05), control of dental equipment and laundry(p<.001), control of infectious waste(p<.05). As above results, in order to minimize the infection prevention in the dental clinic, the regular infection control education for infection control of dental hygienists required, infection control guidelines place at the dental office.

Comparison of the Demographic and Laboratory Profiles of Patients with Aseptic Meningitis and Encephalitis: Significance of Age and C-reactive Protein (무균성수막염과 뇌염환자 사이의 인구학과 검사소견의 비교: 나이와 C-반응단백질의 중요성)

  • Park, Kang Min;Shin, Kyong Jin;Ha, Sam Yeol;Park, Jin Se;Park, Bong Soo;Kim, Sung Eun
    • Annals of Clinical Neurophysiology
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    • v.16 no.2
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    • pp.55-61
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    • 2014
  • Background: Viruses can cause either meningitis or encephalitis. It is unclear why some people suffer from aseptic meningitis, and others acquire aseptic encephalitis when infected with the same viral pathogens. The aim of this study was to compare demographic and laboratory factors between patients with aseptic meningitis and encephalitis. Methods: The demographic and laboratory differences were analyzed according to age, sex, diabetes, hypertension, C-reactive protein in the blood, white blood cell and protein in the cerebrospinal fluid, and glucose ratio (cerebrospinal fluid/blood). Additionally, we analyzed the nation-wide differencesin age between the patients with aseptic meningitis and those with encephalitis in Korea. Results: The patients with aseptic encephalitis were older, more likely to have hypertension, and had higher levels of C-reactive protein than did the patients with aseptic meningitis. However, the numbers of white blood cells in the cerebrospinal fluid were significantly higher in the patients with meningitis than in the patients with encephalitis. Multivariable analysis revealed that age >49 years, hypertension and a C-reactive protein level >5.81 mg/dL were independent and significant variables in the prediction of aseptic encephalitis. Additionally, the patients with aseptic encephalitis were older than those with aseptic meningitis in the nation-wide Korean database. Conclusions: Older age, hypertension, and higher levels of C-reactive protein are useful factors for the prediction of aseptic encephalitis.

Effect of Dietary Protein Level on Caecal Content pH and Serum Protein in Rats to Axenically Cultured Protozoa (식이 단백질 수준의 차이가 흰쥐에서 무균 배양된 원충 감염시에 Caecal Content의 pH와 혈청 단백질에 미치는 영향)

  • 명춘옥
    • Journal of the Korean Home Economics Association
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    • v.30 no.3
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    • pp.91-99
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    • 1992
  • Cell structure is based on proteins. Since the antibody is proteous substance, the continous low protein feeding decreases the resistance of host against pathogenic agents. The present study was designed to investigate the infectivity of protozoa to rats which were fed with variously prescribed diets. Experimental group was divided into 4 groups according to the level of casein in the diet, group I: casein 0%, group II: casein 5%, group III: casein 15%, group IV: casein 30%. Each animal was fed for 5 weeks followed by inoculation of protozoa in cecum and sacrified each 1 week later of the infection. Each diet group, non infected with protozoa was recognized as the control. Result are summerized as follows : 1. All the rats of group I died in 2∼4 weeks and 2 of 12 rats in group II were also died in the period. 2. The growth rate and FER were high in group III and IV compared with group II. Therefore low protein feeding decrease growth and feed efficaly ratio(FER). 3. The pH of caecal contents between the infected group and control showed no difference, but the values of group III and IV were higher than the group II. Low pH of the caecal contents provides a suitable condition for determining their susceptibility to Entameoeba histolytical trophozoite. 4. Amounts of serum total protein in group II, III and IV showed no significant difference with the control and infected group, but amounts in group III and IV were higher than the group II. Therefore, continuous low protein feeding decrease serum total protein. 5. Albumin, ${\alpha}$1, gloulin, ${\alpha}$2 globulin, ${\beta}$ globulin, ${\gamma}$ gloulin of group III and IV were all high to compare than the group II. Albumins of group III and IV of control was higher than infected group, but there was no difference in ${\gamma}$ globulin between the infected and control group.

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Assessment of Occupational Exposure to Antineoplastic Agents in a Healthcare Setting (무균조제 항암제 취급의 안전관리)

  • Lee, Su-Mi;Chung, Seon-Young;Im, Hyun-Jeong;Park, Hyo-Jung;Lee, Su-Yun;Jeon, Eun-Yong;Sohn, Kie-Ho
    • YAKHAK HOEJI
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    • v.55 no.2
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    • pp.81-90
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    • 2011
  • Most antineoplastic agents are nonselective in their mechanism of action, affecting noncancerous as well as cancerous cells, and resulting in acute effects such as irritation of mucous membranes and chronic effects such as genotoxicity, teratogenicity, and carcinogenicity. Healthcare workers occupationally exposed to antineoplastic agents are at risk. The present study aimed to develop and apply methods to monitor occupational exposure to antineoplastic agents, using cyclophosphamide (CP) as the model compound. To monitor environmental and biological exposure, potentially contaminated surfaces were wiped and 24 hour urine samples were collected from workers. Liquid chromatography combined with tandem mass spectrometry was performed, with a limit of detection of 0.05 ng/ml. Measurable amounts of CP were detected on 92% of the sampled surfaces, with a geometric mean of 175.22 $ng/m^2$. Despite the environmental contamination of the model compound, CP was below the detection limit in all urine samples. If workplace contamination cannot be completely avoided, it is importance to reduce exposure to the lowest possible levels. To this aim, efforts to minimize occupational exposure along with biological and environmental monitoring are required. The standardized sampling techniques, and specific and sensitive analytical methods reported in this study may be helpful in assessing occupational exposure and devising strategies to reduce exposure.

PULP REVASCULARIZATION OF IMMATURE FIRST PERMANENT MOLARS WITH APICAL PERIODONTITIS : CASE REPORT (치근단 치주염을 가진 미성숙 제1대구치의 치수 재혈관화 : 증례 보고)

  • Jeon, Hye-Jin;Yang, Yeon-Mi;Kim, Jae-Gon;Baik, Byeong-Ju
    • Journal of the korean academy of Pediatric Dentistry
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    • v.39 no.2
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    • pp.192-198
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    • 2012
  • Revascularization of the pulp in a necrotic, infected immature tooth with apical periodontitis was attempting several years. Revascularization of partially necrotic pulp in an immature tooth is based on the concept that vital dental stem cells can survive pulpal necrosis. Revascularization procedure obtains longer and thicker roots in teeth with necrotic pulp diagnosis. Pulp revascularization for immature permanent molars can be possibly applied on cases having difficulty to use conventional root canal treatment due to abnormally thin root canal wall or severe root curvature. Also, when an uncooperative patient does not agree with sedative treatment the revascularization can be useful. And a patient with disability who is barely cooperative can be another indication of this treatment. In this case report, pulp revascularization using triple-antibiotics, metronidazole, ciprofloxacine and minocycline, was applied on the immature first permanent molar infected by caries.

Genetic Improvement for the Low Salinity-Tolerant Porphyra Sp. by Cell Culture Technique I. Tissue Culture of Porphyra yezoensis foma narawaensis (세포배양기술에 의한 김의 내저염성 품종개량 I. 큰방사무늬김의 조직배양)

  • Hong Yang Ki;Sohn Chul Hyun;Chang Jung Won
    • Journal of Aquaculture
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    • v.2 no.1
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    • pp.1-7
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    • 1989
  • Axenic tissue culture of a marine red algae Porphyra yezoensis foma narawaensis was established for the vegetative propagation of tissues as a seed stock and for the development of a low salinity-tolerant cell line. Callus tissues have been induced from the vegetative area of blade away from the hold fast when grown on PES-agar medium. The brownish red fragile callus was maintained under fluorescent light of ca. 2000 lux with 12 : 12 hr L : D at $16^{\circ}C$. Amounts of carbohydrate and protein was determined against the weight of callus. Optimum temperature of the callus growth was $14^{\circ}C\~18^{\circ}C$. Optimun concentration of sodium chloride was $2.0\%$ for the callus growth in PES-agar medium.

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Seed Infection and Damage to Rice Seeds and Seedlings by Seed-Borne Gerlachia oryzae (벼 갈색잎마름병균(Gerlachia oryzae)의 종자감염과 종자감염이 벼종자 및 유묘에 미치는 피해)

  • Kim Wan Gyu;Park Jong Seong;Yu Seung Hun
    • Korean journal of applied entomology
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    • v.23 no.2 s.59
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    • pp.126-131
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    • 1984
  • Twenty one fungi were detected from 26 rice seed samples obtained from Yeongnam Crop Experiment Station, Honam Crop Experiment Station, Chungnam Provincial Office of Rural Development and farmers of Chungnam Province. Gerlachia oryzae was one of frequently detected fungi with $1.0\~45.0\%$ of detection ratio from 22 rice seed samples. The deep-freezing method was selected as the most suitable for routine seed health testing when the blotter method, deep-freezing method and agar plate method were evaluated for efficiency of detecting seed-borne G. oryzae from rice seeds. Our data obtained by seed component plating technique suggested that G. oryzae was present not only on chaff, in endosperm and in seed coat, but also in the embryo. Seed-borne G. oryzae caused seed rot, seedling blight and symptom of brownish discoloration on coleoptile primary and 2nd leaf when the infected seeds were sown in agar of test tube or in soil.

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Aseptic Germination of F1 Hybrid Seed by Inter-species Pollination of Calanthe discolor Lindl. and C. discolor for. Sieboldii (Decne.) Ohwi (새우난초(Calanthe discolor Lindl.)와 금새우난초[C. discolor for. sieboldii (Decne.) Ohwi]의 종간교배에 의한 1대잡종 종자의 기내무균 발아)

  • Kim, Kwang-Soo;Kim, Jong-Sun;Park, Jong-Hwan
    • Korean Journal of Plant Resources
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    • v.21 no.4
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    • pp.341-345
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    • 2008
  • Two orchid species of Calanthe discolor Lindl. and C. discolor for. sieboldii (Decne.) Ohwi, which have different form flower color and size. They were crossed in mid April by artificial pollination, and the F1 hybrid seeds were collected mid October. Germination of seeds was investigated on pre-treatment of seeds and under the various environmental conditions. Germination was promoted by moisture absorption and ultrasonic treatment of seeds. Dark culture of F1 hybrid seeds enhanced germination and protocorm formation, and development into seedlings compared with light culture. Although, plant growth regulators such as NAA and BA had a slightly promotive effect on seed germination and protocorm growth, regenerated seeding were showed abnormal growth patterns. Regenerated F1 hybrid plantlets were successfully transferred to pot.

Biochemical Studies on Korean Fermented Foods. (IX) Variation of Vitamin $B_{12}$ during the Kimchi Fermentation Period (한국 발효식품에 대한 생물화학적 연구 (제9보) 침채류의 발효에 따르는 $VitaminB_{12}$의 변화에 대하여)

  • Lee, In-Jae;Haw, Kum;Kim, Sung-Ikk
    • YAKHAK HOEJI
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    • v.4 no.1
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    • pp.53-55
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    • 1959
  • In order to confirm on production of vitamin $B_{12}$ during the kimchi fermentation period, the variation of its content is studied in this paper As a sample of kimchi for this fermentation study due to the seasonal condition, nabakkimchi which is aseasonal one in early spring, is prepared by author. The content of vitamin $B_{12}$ is estimated by the microbiological assay method using lactobacillus leichmannii A Tee 7830. Details for assay are indicated in the original part. And the pseudo-vitamin $B_{12}$ substances as thymidine which is active to the growth of lactobacillus leichmannii, are eliminated by the alkali treatment method of sample solution, According to the results shown in table 2 and 3 and figures 1 to 4, the following conclusions are summerized; 1. Vitamin $B_{12}$ produced during the fermentation period of kimchi. And the content of this vitamin during its optimum fermentation period, is much higher content compared with the theoretically calculated amount from its materials, This has been confirmed at the repeated experiment. The trend of variation during its period is indicated at the figures. 2. The sterilized kimchi inhibited the fermentation by sterilization at the preparation, did not increased its content showing only its theoretically calculated amount.

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Bacterial Meningitis during Continuous Epidural Block (지속적 경막외 차단중 발생한 세균성 뇌막염)

  • Lee, Jung-Koo;Chung, Jung-Gil
    • The Korean Journal of Pain
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    • v.7 no.1
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    • pp.113-115
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    • 1994
  • Bacterial meningitis is a rare complication of epidural block. As epidural abscess, subarachnoid infection associated with epidural catheters are related to the treatment of pain in diabetic patient whose immune responses have been impaired. A 51-year-old male with non-insulin dependent diabetes came to the pain clinic with neuropathic gain on right thigh and amputated stump of right leg. Treatment consisted of continuous epidural block and subcutaneous tunnelling and epidural morphine with bupivacaine was given on an outpatient basis. Two months later, the patient noted a diffuse frontal headache, projectile vomiting and stiffness neck. These symptoms became more aggrevated over the following 24 h and temperature went up to $38.4^{\circ}C$. A diagnostic lumbar puncture revealed CSF total protein of 747 mg/dl, glucose of 43 mg/dl, and $4320\;WBC/mm^3$. Cultures of epidural catheter tip grew hemolytic staphylococcus epidermidis. A chest x-ray and brain CT scan were negative. Antibiotic therapy with penicillin G and chloramphenicol was given for 15 days. Recovery was uneventful.

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