• Korean Journal of Plant Tissue Culture
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• v.28 no.6
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• pp.335-339
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• 2001

Grapevine leafroll-associated 3 closterovirus (GLRaV-3) is phloem limited virus, and one of the most severe viral diseases found in Korea. However, nonhomogenous distribution and low concentration and seasonal variations of GLRaV-3 in grapevines remain as main problems which prevent the introduction and molecular biology or serology experiments. Virus-infected plantlet in vitro was obtained from node tissue cuttings, which was GLRaV-3 infected 'kyoho' vines. The amount of purified virus was highest in vitro plantlet. Moreover, viruses seem to be relatively homogeneously distributed in all organs including leaf, stem and callus derived from in vitro plantlets. RT-PCR detection using in vitro plantlet tissue as template was most effective. When comparing ELISA to RT-PCR, RT-PCR detection was 1,000 times as effective as ELISA. These results can be explained by improved quality such as tenderness or less tannins in plantlet in vitro. In conclusion, until infected herbaceous host will be available, tissue culture can be usefully adopted as a technique for a good source of GLRaV-3 closterovirus for further studies.

• Journal of Korean Society of Forest Science
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• v.2 no.1
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• pp.1-4
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• 1962

1. 묘목(苗木)의 포장(包裝) 경과(經過) 일수(日數)와 활착(活着) 관계(關係)를 조사(調査)한즉 표준구(標準區) 즉, 굴취(堀取) 직후(直後) 직재구(直裁區)의 활착률(活着率) 95%에 비(比)하여 2일(日) 경과(經過)까지 90%, 4일(日) 경과(經過)까지 80%, 5일(日) 경과(經過)까지 77%의 활착률(活着率)을 보았다. 2. 수종(樹種)과의 관계(關係)를 보면 아카시아 > 산오리> 리기다 소나무 > 잣나무 낙엽송 순위(順位)로 포장(包裝) 경과(經過)에 견디는 힘이 컸다. 3. 연도별(年度別)(1958, 1959)의 성적차는 인정치 않았다.

• Korean Journal of Medicinal Crop Science
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• v.5 no.3
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• pp.237-242
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• 1997

The experiment was conducted to find causes of rooting failure in divided crown of peony cultivation. The results were as follows : In farmer s fields, damage rate of rooting failure in divided crown of peony was 32% as non-sprouting 5.6%, withering after sprouting 20.7%, and wilting after sprouting 5.7%, repectively. Damage degree in farmer s fields was followed, above 70% by 4%, 41 to 70% by 17%, 11 to 40% by 45% per total field area, respectively. It was caused by rooting failure. Damage rate of rooting failure as affected by different planting time was 18.2% for planting in autumn, 42.9% for planting in spring, and damage in divided crown was higher than in seedling. As periods to planting were prolonged, growth and yield were larger poorly, treatment with seminal-root sterilization and soil insecticide showed good growth and rooting but untreated control was very poor. Main cause of rooting failure in divided crown of peony was disease, Cylindrocarpon sp. and low quality of seeds.

• The Hankook-Saengyark Bo
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• no.252
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• pp.5-5
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• 2002

참당귀 노지육묘때 비가림시설 설치(점무늬병 발생줄고 규격묘 생산량은 증가) - '잔대' 꽃대 8월상순 제거 품질 좋아지고 증수 - 상황버섯 참나무 톱밥배지 사용(관행재배시 보다 수확량 두배 증수) - 농작물 온풍건조기 한약재 건조에 적합 -삼백초 7월말$\cdot$10월말 2번 나눠 수확

• Korean Journal of Plant Tissue Culture
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• v.24 no.6
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• pp.323-327
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• 1997

Addition of growth inhibitors such as ancymidol, ABA, chloromequat, and pachlobutrazol into MS medium had no effect to preventing vitrification in cultures of Rehmania glutinosa. Anatomical investigation revealed that vitrified thick leaf tissue in vitro had larger intercellular space with poor development of sponge and pallisade tissue compared to those of in vitro grown glaucous and field grown plants. In vitro grown glaucous leaf had smaller and round type stomata showing distinguishable guard and subsidiary cell than those of reestablished plantlets into soil whereas abnormal stomata and poor development of epicuticular wax on the surface of leaf was observed in verified plantlet.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.42 no.6
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• pp.678-686
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• 1997

This experiment was carried out to develop the mass propagation system for producing plug plantlets using stem cuttings of virus-tree microtubers in potato. Cocopeat, vermiculite, perlite and peatmoss were combined and used as plug nursery media to find out the best combination suitable for the growth of seedlings derived from microtubers. Seedling growth was favored in high temperature (above 2$0^{\circ}C$) and a long-day photoperiod(above 16 hours) condition, while stolons and microtubers formed in outdoor condition. Shoot and root multiplication was not affected by NAA 10mg /1 or IAA 10mg /1 treatment. At the early growth stage of plug plantlets, the number of leaves and roots and the length of root increased significantly when nodes from the upper (near to apex) part of shoots rather than from basal part were taken. But after transplanting, these differences among these characters were not observed. At ninety days after transplanting the plug plantlets in spring time, plant was around 70 to 80cm in height, and the number of stolons and tubers were ten and seven, respectively.

• Journal of Sericultural and Entomological Science
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• v.31 no.2
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• pp.69-71
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• 1989

Experiments were conducted to investigate the effect of topping from mulberry graftages on the their size. Topping season was examined at 10 days intervals from July 15 to August 5 1986 and 1987 on the paddy and upland, respectively. The results were : 1. Topping increased the passed rate of graftages 3.0-15.9%, compared with the control. 2. Optimum season of topping fell around July 15. 3. Topping showed no difference to branch length, survival rate and shoot length in the next year. 4. There was no difference between paddy and upland nursery garden in the passed rate.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.23 no.2
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• pp.118-125
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• 1978

To find out highly resistant gene source to black rot fungus in sweetpotato varieties, a screening test was carried out with inoculated tubers and sprouts in 1976 and 1977. Fifteen varieties out of 165 and 54 varieties out of 161 were highly resistant to black rot in the tuber and sprout tests. respectively. The sprout test showed bigger variation from year to year compared to tuber test. Varieties highly resistant to black rot in both tuber and sprout tests were Suweon # 59, #81, #90, Norin #17, #23. Chilship-il cho, Sachun Jong #36, Hamkao, and Kandee.

• Horticultural Science & Technology
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• v.29 no.3
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• pp.273-277
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• 2011

Fusarium oxysporum f. sp. fragariae (Fof), the causal agent of crown and root rot in strawberry, is the most serious soilborne disease of nursery plants in Korea. The possibility of infection by Fof through runner propagation from infected mother plants of strawberry cv. 'Kumhyang' was assessed in stolons and daughter plants hanging from raised beds. The number of daughter plants from an infected mother plant in plastic house and photosynthetic photon flux (PPF) system, 280 ${\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ was 2.7 and 3.8 plants after 58 days, respectively. However, healthy mother plants produced 6.5 and 8.4 daughter plants, respectively. The pathogen was detected in the uppermost portion of the stolon after 58 days, but was not detected further down the stolon. After 90 days, it was detected in all portions of the stolon between mother and $1^{st}$ daughter plant and in 60% of all $1^{st}$ daughter plants. The pathogen was not detected in the corresponding portions of the non-infected controls. These results show that infected mother plants can transmit Fof to their daughter plants without passing through the soil and $1^{st}$ daughter was used as mother plant in PPF system for propagating healthy plants.

• Korean Journal of Plant Tissue Culture
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• v.26 no.3
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• pp.205-209
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• 1999

Chinese foxglove(Rehmannia glutinosa) is receiving much attention as one of the principal medicinal crops and the crud drug. This study was conducted to obtain the basic breeding information of Chinese foxglove derived from tissue culture. To compare plant characteristics between local variety and tissue cultured seedlings, plant growth and root yield has been investigated. In addition, catalpol and free sugar contents were also analized. The ability for the storage of root stock originated from tissue culture seedlings were better than that of local variety. The growth and root yield of in vitro propagated plants were superior to those of conventionally propagated plants. Root yield of 1-year-old and 2-year-old seedlings investigated 112% and 246% respectively than Seacheon local shows 384 kg/10 a. Although there were no significant different between tissue culture seedlings and conventionally propagated one, slight decline of component contents in tissue culture plants were still existed. Investigated sugar content among Seocheon local, 1-year-old tissue culture seedlings, and 2-year-old tissue culture seedlings were 1.86%, 1.21%, and 1.10% respectively. Catalpol content as one of standard materials indicates 0.46% in Seocheon local and 0.40% in tissue cultured ones.