• The Bimonthly Magazine for Agrochemicals and Plant Protection
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• v.7 no.4
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• pp.97-109
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• 1986

우리나라의 주곡생산은 다수확 신품종의 보급과 재배기술의 발전으로 어느정도 안정상태에 이르고 있다. 그러나 병해충의 발생과 기상재해는 식량자급에 가장 큰 문제점이 되고 있다. 따라서 미곡생산을 안정시키고 지속적인 다수확을 이루기 위해서는 병해충에 대한 저항성 품종의 육성과 아울러 병충해의 적절한 종합방제 체계의 확립이 선행되어야 하겠다.

• Korean journal of applied entomology
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• v.56 no.4
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• pp.377-385
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• 2017

Estimates of evolutionary sequence divergence and inference of a phylogenetic tree for eight delphacid planthopper species were based on the full-length nucleotide sequence of the internal transcribed spacer 2 (ITS2) region. Size of the ITS2 DNA sequence varied from 550 bp in Sogatella furcifera to 699 bp in Nilaparvata muiri. Nucleotide sequence distance ($d{\pm}S.E.$) was lowest between N. muiri and N. bakeri ($0.001{\pm}0.001$), and highest between Ecdelphax cervina and Stenocranus matsumurai ($0.579{\pm}0.021$). Sequence distance between N. lugens and other planthoppers ranged from $0.056{\pm}0.008$ (N. muiri) to $0.548{\pm}0.021$ (S. matsumurai). In the neighbor-joining phylogenetic tree, all planthoppers were clustered separately into a species group, except N. muiri and N. bakeri. The ITS2 nucleotide sequence of N. lugens was used to design four loop-mediated isothermal amplification (LAMP) primer sets (BPH-38, BPH-38-1, BPH-207, and BPH-92) for N. lugens species-specific detection. After the LAMP reaction of three rice planthoppers, N. lugens, S. furcifera, and Laodelphax striatellus, with the four LAMP primer sets for 60 min at $65^{\circ}C$, LAMP products were observed in the genomic DNA of N. lugens only. In the BPH-92 LAMP primer set, the fluorescence relative to that of the negative control differed according to the amount of DNA (0.1 ng, 10 ng, and 100 ng) and incubation duration (20 min, 30 min, 40 min, and 60 min). At $65^{\circ}C$ incubation, the difference was clearly observed after 40 min with 10 ng and100 ng, but with a 60-min incubation period, the minimum DNA needed was 0.1 ng. However, there was little difference in fluorescence among all DNA amounts tested with 20 or 30 min incubations.

• Korean journal of applied entomology
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• v.20 no.3 s.48
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• pp.173-175
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• 1981

New screening method of varietal resistance by isotope $^{32}P$ was examined in these experiments. Insects were fed for 24 hrs. in the vials, with rice seedlings absorbed the solution if $0.6\~7{\mu}\;Ci\;^{32}P$ for 24 to 48 hrs. Feeding amounts of 3 species of plant-hoppers at the different stages and duration of infestation were investigated for effective process of screening method of the varietal resistance using isotope $^{32}P$. Feeding amount of $^{32}P$ of brown plant-hopper was observed for the different varieties. The results may be summarized as follows: 1. Brown planthopper fed greatest and the feeding amount were increased in order of white-backed planthopper and small brown planthopper. Female hoppers fed more than male. 2. Feeding amount was increased in order of adult, 5th instar, adult, 4th instar, 3rd instar 2nd instar and 1st instar. The duration of $24\~48\;hours$ is considered sufficient for insect infestation. 3. New screening method by $^{32}P$ was compared with seedling bulk screening method in view of feeding amount and plant reaction. Feeding amount of $^{32}P$ by brown planthopper in Milyang 47, resistant variety to this insect, was very low, while in TN 1, susceptible variety, it was very high about one hundred tines of Milyang 47.

• Proceedings of the Korean Society of Applied Entomolohy Conference
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• 2004.05a
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• pp.65-65
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• 2004

• Proceedings of the Korean Society of Applied Entomolohy Conference
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• 2001.11a
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• pp.58-58
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• 2001

• Korean journal of applied entomology
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• v.29 no.1
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• pp.1-5
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• 1990

A morphological study on the immature and adult stages of Anagrus incarnatus Haliday was carried out under laboratory condition at 25$\pm$1$^{\circ}C$. The egg is sausage-shaped with a long slender pedicel. The first instar is a bag-shaped larva. The second instar larval form of A. incarnatus is similar to he "histriobdellid" stage. The antenna has 9 and 13 segments of the female and male, respectively. The first funicular segment of the female's antenna is very short, subspherical or globular type and the third funicular segment with one sensory ridge is longer than the forth. The forewing of A. incarnatus has 8-9 rows of discal hairs providing no bare space at the broadest part. The length of ovarian eggs, egg, 1st instar, and 2nd instar of A. incarnatus is 0.165, 0.215, 0.290, and 0.535 mm, respectively, and the width of each stages is 0.025, 0.057, 0.082, and 0.110 mm, respectively. The developmental periods for egg, 1st instar, 2nd instar, prepupa, and pupal stage are about 1, 1, 4, 1, 5-6 days, respectively.pectively.

• Korean journal of applied entomology
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• v.5_6
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• pp.55-58
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• 1968

본시험은 1966년 필리핀에 있는 국제수도연구소에서 벼멸구의 생태에 관한 시험을 위하여 벼멸구를 대량으로 집단접종사육하여 오딘 중 cage내 갑자기 벼멸구의 밀도가 줄고 장님노린재가 무수히 번식되고 있는 것을 관찰한 후 Hawaii에 있는 Bishop Museum, Dr. ASHLOCK 씨에이 장님노린재에 대한 동정을 의뢰함과 동시에 벼멸구에 대한 포식성을 조사한 결과 벼멸구의 포식식능력은 장님노린재를 산난된 도엽에 접종하였을 때 5일후 포식난율이 $69.1\%$인대 대하여 벼멸구 고충에 대하여서는 접종 1일 후$61.4\%$, 2일 후 $76.2\%$, 3일 후 $91.0\%$ 4일 후 $93.3\%$의 포식률로서 매우 놀라운 포식능력을 보여주었다. 이 장님노린채는 벼멸구뿐만 아니라 다른 멸구류의 밀도를 제압할 수 있기 때문에 단장조건하에서는 벼멸구류의 피해를 미연에 경감시킬 수 있는 유리한 천적이라고 생각되며, 금후 이의 실제적인 이용면에 대한 연구가 기대된다.

• Korean journal of applied entomology
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• v.34 no.3
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• pp.234-242
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• 1995

The functional responses of the female Cyrtorhinus lividipennis on brown planthopper (BPH), Nilaparvata lugens, eggs and their predation behavior were investigated at six temperature conditions; 20, 23, 26, 29, 32, and $35^{\circ}C$. C. lividipennis was found to prefer young BPH eggs, especially 3-day-old eggs the most. The functional responses for female C. lividipennis on BPH eggs fitted the Holling's Type-I true predator-prey Rogers' (1972) model at all temperatures tested except $35^{\circ}C$ at which the negative Th value was produced. With the temperature increased up to 32$^{\circ}C$, the instantaneous attacking rate(a) increased from 0.1923 at $20^{\circ}C$ to 0.5085 at $32^{\circ}C$, while the handling time (Th) was gradually decreased as low as 0.0151 at $32^{\circ}C$. C. lividipenis preferred the BPH eggs laid on the upper part of rice stem when the BPH egg density was high, but there was no significant difference in the preference when the egg density was low. The preference was more obvious in high temperature conditions such as above 29$^{\circ}C$.

• Korean journal of applied entomology
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• v.21 no.2 s.51
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• pp.87-94
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• 1982

The mymarids egg parasite of rice planthopper, Anagrus nr. flaveolus, were investigated to know their parasitic activities after overwitering in the paddy banks and barley fields, their host preferences and seasonal variations in the pesticide sprayed and unsprayed paddy fields of Gyeongnam province O.R.D. at Jinju from 1977 to 1979. The parasitic activities of Anagrus nr. flaveolus after overwintering in the paddy banks were high early in April and tended to decrease remarkably since mid-April by moving to the barley fields. The parasitic rate of Anagrus nr. flavelous was $47.2\~88\%$ between middle and late in April, the peak of egg deposition period. Anagrus nr. flaveolus parasitized Laodelphax striatellus, Nilaparvata lugens, and Sogatella furcifera, but didn't attack the eggs of Nephotettix cincticeps in the paddy fields. High preference was observed with Laodelphax steriatellus. The parasitic activities of Anagrus nr. flaveolus in the pesticide sprayed paddy fields were high in early July and from late August to early September. The parasitic rate in the pesticide unsprayed fields were higher than those of sprayed fields during the pesticide spraying period, from July to August and parasitic activities were active from October to before coming winter.

• Korean journal of applied entomology
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• v.15 no.3 s.28
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• pp.111-126
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• 1976

Regional differences in economic importance of the four species of rice leafhoppers, viz., Laodelphax striatellus, Nephotettix cincticeps, Nilaparvata Iugens and Sogatella furcifera, were analyzed and discussed based on population surveys conducted in Suweon and in southern and south-western coastal areas. The economic importance of L. striatellus and N. cincticeps, which are found throughout the year in Korea, seems to be less in middle regions. This is believed due to effective natural control through severe winter climate, cultural practices in rice and barley growing, and natural enemies such as spiders. However, the economic importance of these two species is significantly greater in southern regions with a less severe winter climate, and where cultural practices in rice and barley favour synchronization between the life cycles of insects and the seasonal succession of host plants. with regard to N. lugens and S. furcifera, which cannot overwinter in Korea, initial populations are the result of long distance migration across the East China Sea. Weather elements related to their migration result in more abundant immigrants of these two species in southern than in middle regions. Thus, different policies are needed for the integrated control of rice leafhoppers in middle and southern Korea.