• Korean journal of applied entomology
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• v.56 no.4
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• pp.377-385
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• 2017

Estimates of evolutionary sequence divergence and inference of a phylogenetic tree for eight delphacid planthopper species were based on the full-length nucleotide sequence of the internal transcribed spacer 2 (ITS2) region. Size of the ITS2 DNA sequence varied from 550 bp in Sogatella furcifera to 699 bp in Nilaparvata muiri. Nucleotide sequence distance ($d{\pm}S.E.$) was lowest between N. muiri and N. bakeri ($0.001{\pm}0.001$), and highest between Ecdelphax cervina and Stenocranus matsumurai ($0.579{\pm}0.021$). Sequence distance between N. lugens and other planthoppers ranged from $0.056{\pm}0.008$ (N. muiri) to $0.548{\pm}0.021$ (S. matsumurai). In the neighbor-joining phylogenetic tree, all planthoppers were clustered separately into a species group, except N. muiri and N. bakeri. The ITS2 nucleotide sequence of N. lugens was used to design four loop-mediated isothermal amplification (LAMP) primer sets (BPH-38, BPH-38-1, BPH-207, and BPH-92) for N. lugens species-specific detection. After the LAMP reaction of three rice planthoppers, N. lugens, S. furcifera, and Laodelphax striatellus, with the four LAMP primer sets for 60 min at $65^{\circ}C$, LAMP products were observed in the genomic DNA of N. lugens only. In the BPH-92 LAMP primer set, the fluorescence relative to that of the negative control differed according to the amount of DNA (0.1 ng, 10 ng, and 100 ng) and incubation duration (20 min, 30 min, 40 min, and 60 min). At $65^{\circ}C$ incubation, the difference was clearly observed after 40 min with 10 ng and100 ng, but with a 60-min incubation period, the minimum DNA needed was 0.1 ng. However, there was little difference in fluorescence among all DNA amounts tested with 20 or 30 min incubations.

• Korean journal of applied entomology
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• v.62 no.2
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• pp.57-60
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• 2023

In this study, the genus Tagosodes Asche and Wilson, 1990 is reported for the first time in South Korea. Species distribution, measurement, host plants, description, and illustrations of diagnostic characters of Tagosodes pusanus Distant, 1912 are provided.

• Korean journal of applied entomology
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• v.25 no.1 s.66
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• pp.21-25
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• 1986

Number of small brown planthoppers, Laodelphax striatellus(F.) negatively correlated with the mean temperature of March in Korea. The low temperature of March delayed the barley growth, heading date, and maturity. Thereby, was small brown planthopper was given a good condition to feed barley, and caused abundant occurence in 1984. In addition, it was possible to predict the occurence ot small brown planthopper by quadractice equations.

• Korean Journal of Breeding Science
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• v.41 no.3
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• pp.236-243
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• 2009

This study was conducted to develop a japonica-type rice cultivar with brown planthopper (BPH) resistance using DNA markers. A doubled haploid (DH) population consisting of 120 pure-lines was established by anther culture of $F_1$ hybrids between 'Samgang', a Tongil type BPH resistance cultivar, and 'Nagdong', a japonica cultivar. To determine the map position of genes responsible for BPH resistance in rice, a genetic map was constructed based on 120 DH lines. A total of 162 molecular markers were classified into 12 linkage groups, covering 1,884 Kosami centimorgan (cM) with an average of 11.6 cM. Five QTLs (qBPR3, qBPR6, qBPR7, qBPR8, and qBPR12) associated with BPH resistance were identified and mapped on chromosomes 3, 6, 7, 8, and 12, respectively, using the genetic map constructed in this study. To analyze the relationship between BPH resistance and agronomic traits, a total of eight QTLs related to the agronomic traits were detected on 12 rice chromosomes. In an analysis of relationships, three QTLs (qBPR3, qBPR7, and qBPR8) showed a linkage with tested agronomic traits. A QTL (qBPR3) located on chromosome 3 (RM282-3023) was closely linked to culm length (qCL3). The QTL (qBPR8) for BPH resistance on the short arm of chromosome 8 also overlapped the region detected in culm length (qCL8).

• Korean journal of applied entomology
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• v.33 no.4
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• pp.270-280
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• 1994

This study was conducted to establish a nce insect pest control system, ~ es.ys tematic application of insecticides using carbohran and buproiezin, in Korea. The effects oi various dates of application and rates of buprofezin (25% WP) after carbofuran (3G) soil incorpombon in late May on the pop~~lation densities of the brown planthoppa (BPH) immigrating in July were investigated Appropriate application tune of buprofezin for the BPH that had evaded insecticidal effect of 5011 incorporated carbofuran was late July-early August. Application rate of buprofezln at 7.0g a.i/lOa was enough to suppress the BPH density. Buprofezin treatment after carbofumn soil incorporation could also suppress the whlte backed planthopper population but did not affect the densities oi the paddy rice spiders. Considering the charactenstics of occurring patterns of the nce insect pests in Korea. buprofezin treatment m late July or early August after carbofuran soil incorporation in late May can be a useful application system of ir~sectic~deins controlling early season Insect pests and migmtoly planthoppers on rice.

• Korean Journal of Agricultural Science
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• v.12 no.2
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• pp.253-259
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• 1985

This paper presents a natural enemy complex of plant and leafhoppers in rice plants and a relationship between the host insects and their natural enemies. Feeding preference of major paddy spiders have been tested. To date, 32 species of natural enemies of plant and leafhoppers have been recorded, including 30 species of parasites and predators (15 parasitoids, 14 predators and nematode) and 2 species of insect pathogens. Some of species have shown great potential for controlling plant-leafhoppers populations; parasites, Anagrus nr. flaveolus, Gonatocerus sp., Haplogonatopus atratus and paddy spiders. Pirata subpiraticus and Gnathonarium dentatum. Plant and leafhoppers-natural enemy relationship have been observed in different developmental stages of the host insects. Pirata sub-piraticus is the most active to searching the host insects and also prefered nymphs to adults.

• Korean journal of applied entomology
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• v.41 no.2
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• pp.75-83
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• 2002

The hypertext-based polychotomous key in m (World Wide Web) was developed to improve the identification accuracy of planthoppers caught by the light trap in Korean paddy fields. The effects of it were tested by 12 students who are not familiar at the identification of insects. When they used the hypertext, it was improved that the ability of them to recognize Sogatella furcifera (Horvath) and Laodelphax striatellus (Fallen). Identification accuracy of the former was increased significantly from 56% to 83% and that of the latter was also increased significantly from 47% to 80%. However, many students still have difficulty in the recognition of Nilaparvata lugens (Stal).

• Korean journal of applied entomology
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• v.22 no.4 s.57
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• pp.244-250
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• 1983

This study was carried out to analyst the damage due to the brown planthopper, Nilapalvata lugens Stal, at different stages(maximum tillering, booting, heading, and milking) of rice. The results are as follows: The population density of the migrating and the first generations of the brown planthoppers seemed not to be large enough to cause significant effects on the plant height and the number of tillers, while the second and the third generations caused significant reduction in 1000 grain weight(Yl) and the filled grain percentage$(Y_2)$. They were very closely related to the population density(X) of tile second generation of the BPH; $$Y1(g)=25.602-0.0213X(r=-0.5205^{**})$$ $$Y2(\%)=76.97-0.1370X(r=-0.4820^{**})$$ The relationship between rice yield (Yg) and the accumulated total density (X) of BPH per hill by the time of harvest; $$Y(g)=24.694-0.0576X(r=-0.6959^{**})$$ Since the source of the outbreak BPH is the first generation, it nay conclude that the detailed information on the population status of the first generation; density, age components, ratio of the brachypterous form, are very important in relation to management of the pest.

• Korean Journal of Breeding Science
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• v.43 no.1
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• pp.56-61
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• 2011

Brown planthopper(BPH) is one of the most destructive insect pests of rice. The use of genetically resistant cultivars has proven to be a more economical, efficient and environment friendly means to combat this pest. This study was carried out to investigate the relationship between BPH resistant gene, Bph18 and yield components of rice using DH(doubled haploid) lines derived from 'Saenuri'/SR30071-3-7-23-6-1-1-1. SR30071-3-7-23-6-1-1-1 line has Bph18 gene derived from wild species, Oryza australiensis. BPH resistant gene, Bph18 shortened heading days, enlarged culm length and panicle length and reduced ratio of ripened grains of rice. The results indicate that backcrossing breeding is necessary to develop elite cultivars carrying Bph18 gene.

• The Korean Journal of Pesticide Science
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• v.10 no.2
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• pp.124-130
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• 2006

Rice plant extracts of brown planthopper (BPH) resistant rice varieties, Jangseongbyeo (JSB) and Hwacheongbyeo (HCB) at different growth stages (seedling, tillering, heading and ripening) were sequentially fractioned using hexane, ethyl ether, ethyl acetate, butanol, and distilled water. The extracts were applied to BPH susceptible rice variety, Dongjjnbyeo (DJB), to investigate the insecticidal and repellent effects against BPH. BPH insecticidal effects were not clearly observed with almost all of the extract fractions obtained from both JSB and HCB varieties for 12 h, whereas the ethyl ether and hexane extract fractions showed about 10 to 30% of BPH mortality in 24 to 48 h of application periods. An effective BPH repellent activity was found with the applications of ethyl ether extract fractions obtained from JSB variety. The extract fractions obtained from HCB variety did not show any different repellence among the various fractions. The BPH repellent effects of the extract fractions obtained at different growth stages of either JSB or HCB varieties did not show any correlations. The effect of ethyl ether fraction on BPH repellent was continually increased by 30 h after treatment and thereafter decreased. In addition, the first sub-fraction separated by a flash column chromatography eluted with chloroform:methanol (9:1, v/v) from the BPH effective ethyl ether faction in JSB variety might be meaningful to repel BPH from BPH susceptible target rice plants. The results indicated that the ethyl ether fraction obtained from JSB was higher in repellent activity than in insecticidal activity, and suggesting that there might be specific substance(s) in the first sub-fraction (sF1) of the ethyl ether fraction in JSB that could provide repellent activity against BPH.