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Mosquito Distribution and Detection of Flavivirus Using Real Time RT-PCR in Jeju Island, 2017 (제주지역 모기의 계절적 발생소장 및 Real Time RT-PCR을 이용한 Flavivirus 감염조사(2017))

  • Lee, Che-Wook;Hwang, Kyu-Kye
    • Korean journal of applied entomology
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    • v.57 no.3
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    • pp.177-183
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    • 2018
  • This study examined the seasonal, regional distribution of mosquito vectors related with disease vectors in the Jeju. From March to November, sample were collected from 11 points in four environmentally different sites in Jeju Island. Samples were collected twice a month using a black-light trap and a BG sentinel trap. Overall, five genera and seven species types of 6,042 female mosquitos were collected. Among the collected mosquitos, 4,159 (68.8%) and 1,348 (24.4%) were Culex pipiens and Aedes albopictus, respectively, making them the dominant species. Additionally, collection using the black light trap produced 72.8 entities per trap in Jungang-dong service center in the center of the city, which was the highest value, while the lowest amount of 1.4 per trap was recovered from the airport. When the BG sentinel trap was used, the largest recovery was observed in the port, where there were 71.7 entities per trap, while the lowest amount of 28.3 entities per trap was recovered at Gealmae Eco Park. The overall number of mosquitoes collected started to increase from May, and reached the largest value of 1,156 (19.1%) in August. Trapped mosquitoes are created 364 pools of up to 50 grains per pool, by season, by environmental, and by species. When the pools were used, no flaviviral infection was observed upon real time RT-PCR.

Detection of Borrelia burgdorferi and Ehrlichiosis Agent in Ticks Collected in Korea Using Polymerase Chain Reaction (국내에서 채집한 진드기에서 중합효소연쇄반응을 이용한 라임병균 및 Ehrlichiosis 원인체의 검출)

  • 김종배;송혜원;박성언;박상욱;안준환;엄용빈;김영미
    • Biomedical Science Letters
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    • v.4 no.2
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    • pp.113-120
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    • 1998
  • To investigate the distribution of Borrelia burgdorferi and human granulocytic ehrlichiosis (HGE) agent in ticks, adult ixodid ticks of Ixodes spp. and Haemaphysalis spp. were collected from the high mountain areas of Kangwon Province. Using DNAs extracted and purified in the collected ticks, polymerase chain reaction (PCR) was performed to amplify the specific nucleotide sequences of both agents. Of the 516 ticks, a total of 68 (13.2%) ticks was positive for Borrelia burgdorferi sensu lato with PCR analysis (2 for B. burgdorferi sensu stricto; 1 for B. afzelii;33 for B. garinii; 8 for B. tanukii;4 for B. turdae). However a little more than half of PCR-positive ticks (37/68) was found to be positive in the southern blot analysis with Bl6S oligonucleotide probe. One hundred and one (19.2%) ticks were positive for Ehrlichia spp. in PCR, and a quarter of them (25/101) was positive in southern blot with El6S oligonucleotide probe. But none of them was found to be the DNA of HGE agent. And 0.6% (3/516) ticks were positive for both of B. burgdorferi sensu late and Ehrlirhia spp. These findings might implicate the possibility of the outbreak of Iyme borreliosis and ehrlichiosis in Korea, and more extensive studies may be need for the diagnosis of multiple tick-borne diseases.

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The first discovery of larval Gnathostoma hispidum (Nematoda: Gnathostomidae) from a snake host, Agkistrodon breuicaudn (한국산 살모사에서 최초로 발견한 돼지악구충의 제3기 유충)

  • 손운목;이순형
    • Parasites, Hosts and Diseases
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    • v.36 no.2
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    • pp.81-90
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    • 1998
  • The present study was performed to observe the infection status of several kind of animals with indigenous Gnnthostomn in Korea, and morphological characteristics of gnathostome larvae detected from pit-viper, Askistronon breuiccudus, for the species bullfrogs. several kinds of snakes, j.e., 55 Elnphe rufodorsata, 2 Dinodon rufozonatum rufzonatum. 62 Rhnbdophis tisrinus tigrinus and 87 Askistronon app., and 438 cats were examined. A total of 21 larval gnathostomes was detected from 12 pit-vipers, A. breuicnudus. They were 2.233 x 0.343 mm in average size and covered with about 210 transverse rows of minute cuticular spines. Their characteristic head bulbs were provided with 4 rows of hooklets of which average numbers in each row were 36.8, 39.0, 41.7 and 44.3. posteriorly. In the cross sections of midgut level, the intestinal wall consisted of a single layer of 19-25 elongate epithelial cells with a single nucleus. SEM observation of the larvae revealed unique features of head bulb , cuticular spines on transverse striations and a cervical papilla. On the basis of above morphological characteristics, they were identified as the advanced third-stage larvae of Gnothostomo hispinum. It was first confirmed that the pit-viper. ABkistronon breuicaunus is the snake intermediate host of G. hispidun.

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Genetic Species Identification by Sequencing Analysis of Nuclear and Mitochondrial Genes for Albino Misgurnus Species from Korea (우리나라 미꾸리속(genus Misgurnus) 알비노 개체의 미토콘드리아 및 핵 유전자 염기서열 분석에 의한 유전적 동정)

  • Song, Ha-Youn;Moon, Shin-Joo;Kim, Keun-Sik;Bang, In-Chul
    • Korean Journal of Ichthyology
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    • v.29 no.2
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    • pp.139-145
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    • 2017
  • The spontaneous color mutant, albino individuals of genus Misgurnus, are rarely discovered in Korea and there are difficult to identify morphological species due to lack melanin pigmentation. In this study, we developed a genetic identification method for the species of albino Misgurnus individuals based on phylogenetic analysis by using recombination activating gene 1 (rag1) and cytochrome b (cytb) region of mitochondrial DNA. As a result of molecular phylogenetic analysis, three clades were identified as Misgurnus mizolepis, M. anguillicaudatus and M. mohoity. The homology of the cytb sequences of M. mohoity was best match to that of M. mohoity sequences in GenBank database. As a result of species identification of 25 albino Misgurnus individuals based on the phylogenetic tree, the red-eye type was identified as 16 M. anguillicaudatus and one M. mizolepis. The remaining three individuals were identified as one M. mizolepis ♀${\times}$M. anguillicaudatus ♂, and two M. mohoity ♀${\times}$M. anguillicaudatus ♂, respectively. In addition, the five black-eye type individuals were identified as one M. anguillicaudatus, three M. mizolepis and one M. mohoity. Therefore, this genetic identification method will be an useful techniques for species or hybrid identification in genus Misgurnus.

Ten Human Cases of Fibricola seoulensis Infection and Mexed One with Stellantchasmus and Metagonimus (Fibyicola seoulensis감염 10례 및 Htelluntchasmus falcatus감염 1례)

  • Hong, Seong-Tae;Chae, Jong-Il;Lee, Sun-Hyeong
    • Parasites, Hosts and Diseases
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    • v.24 no.1
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    • pp.95-97
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    • 1986
  • 서울에 소재한 육군장병의 검변에서 Fibricola의 충란 양성자 10명을 관찰하여 프라지관텔로 치료하고, 이 증 한례에서 Fibricola seoulensis의 성충 59마리, 요꼬가와홉충 68마리 및 Stellantchasmus fnlcatus 2마리를 관찰하였다

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2013년 양계전망 - 한국농촌경제연구원 2013년 산란계 전망

  • Jang, Seong-Yeong
    • KOREAN POULTRY JOURNAL
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    • v.45 no.3
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    • pp.118-120
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    • 2013
  • 지난 1월 23일 한국농촌경제연구원에서 '농업전망 2013'을 발표했다. 이날 발표된 산란계 전망은 산란종계 입식 마리수 감소와 병아리 분양 제한 협의로 2013년 산란계 사육 마리수는 전년 대비 0.8% 감소한 6,147만 마리로 전망, 이후 2014년부터 산란계 사육 마리수는 완만한 증가세를 보일 것으로 예측했다. 또한 계란 생산량 증가로 2013년 계란 산지가격은 전년보다 5.7% 하락한 1,065원(특란 10개)으로 전망했다. 본고에서는 '2013년 산란계 전망대회'에서 발표된 자료 중 주요 내용을 발췌해 양축인들의 이해를 돕고자 한다.

사양 - 육계 육추관리의 5가지 기준

  • Yu, Jae-Seok
    • Monthly Korean Chicken
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    • v.15 no.2
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    • pp.84-89
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    • 2009
  • 모든 원자재 가격이 오르면서 육계산업의 핵심요소 중 하나인 병아리 생산비용이 수당 550원 이상이 되었다. 이러한 현실은 많은 것을 의미하는데 그중 하나가 병아리 한 마리 한 마리의 가치가 높아졌다는 것이고, 한 마리의 병아리라도 소홀할 수 없는 개체개념이 생겼다는 것이다. 육계의 특성상 대군 밀집사육을 하다 보니 소나 돼지처럼 한 마리의 개체개념이 상대적으로 약한 편이지만 이제는 개체관리에도 신경써야 할 시기가 왔다고 판단된다. 육계의 사육일정이 단축되어 29일령에 1.6kg이상 출하해도 놀라운 일이 아닐 정도로 육계사육의 증체속도도 매우 빨라졌다. 따라서 육추기간 동안의 사육 상태에 따라 출하성적 전체에 영향을 주고 있다는 것이다. 이러 이유로 필자의 원고방향도 육추관리에 더 많은 관심을 갖게 된 것이다. 이번 2월호에서는 농장마다 조건이 모두 다르기 때문에 육추방법이 같을 수는 없지만 육추관리에서의 5가지 기준은 어느 농장이나 동일한 기준을 준수해야 한다. 그 기준을 알아보고자 한다.

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In Vivo Experiment of Tissue-Engineered Artificial Vessel (조직공학적 인조혈관의 생체 내 이식 실험)

  • 임상현;조승우;홍유선;김병수;유경종;장병철;최차용
    • Journal of Chest Surgery
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    • v.37 no.3
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    • pp.220-227
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    • 2004
  • The number of patients with coronary artery disease and peripheral vascular disease are increasing, and the need of small diameter vessel is also increasing. We developed small diameter artificial vessel and experimented in vivo. We got allogenic valve from mongrel dogs, and removed all cells from the allogenic valve. Then, we seeded autologous bone marrow cells onto the decellularized scaffold. After implantation of artificial vessel into the canine carotid artery, we performed angiography regularly. In case of vessel occlusion or at 8 weeks after operation, we euthanized dogs, and retrieved the implanted artificial vessels. Control vessels were all occluded except one (which developed aneurysmal dilatation). But autologous cell seeded vascular graft were patent by 4 weeks in one, by 6 in one and by 8 weeks in two. Histologic examination of patent vessel revealed similar structure to native artery. Tissue-engineered vascular graft manufactured with decellularized allogenic matrix and autologous bone marrow cells showed that tissue engineered graft had similar structure to native artery.

Susceptibility of various animals to Pneumocystis carinii infection (카리니주폐포자충 장염에 대한 여러 포유동물 및 흰쥐 계통에 따른 감수성)

  • Hong, Sung-Tae;Park, Kyeh-Hyun;Lee, Soon-Hyung
    • Parasites, Hosts and Diseases
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    • v.30 no.4
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    • pp.277-282
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    • 1992
  • Pneumocystis carinii (Pc) is an important opportunistic pathogen of immune compromised hosts, and is known to infect various animals. The present study observed the infection status of 6 mammals and 3 strains of albino rats with Pc after suppression of their immunity. Methyl-prednisolone was injected once a week and tetracycline was supplied with water for 5 to 21 weeks. Hamsters, guinea pigs, rabbits, dogs, cats and pigs were negative by impression smear, and only the rats were found infected by Pc. All of the three strains of rats, Sprague-Dawley(SD), Wistar(W) and Fisher(F), were infected by Pc but W rats showed heavier degree of infection in earlier period than F or SD rats. The present findings suggest that W rat is the best among the animals used in the present study for production of Pc.

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Radiography and Computed Tomography in Four Dogs with Lung Lobe Torsion (흉부 방사선 촬영과 컴퓨터단층촬영을 이용한 폐염전의 진단 4례)

  • Lee, Ki-Ja;Choi, Sung-Jin;Kim, Young-Hwan;Jeong, In-Seong;Choi, Ho-Jung;Lee, Young-Won
    • Journal of Veterinary Clinics
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    • v.30 no.5
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    • pp.390-393
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    • 2013
  • This case study describes the imaging features of radiography and computed tomography (CT) in four small breed dogs with lung lobe torsion. The medical records, thoracic radiographs and CT images of four dogs with confirmed lung lobe torsion were retrospectively reviewed. Pleural effusion and increased lobar opacity/density were seen on all CT and thoracic radiography in all four dogs. CT revealed narrowed/collapsed bronchus in all four dogs, while this was only appreciated on one thoracic radiography. The vesicular emphysema pattern was seen on four CT but only on three radiographic examinations. The specific findings of lung lobe torsion were vesicular emphysema and a narrowed/collapsed bronchus. These findings were more easily recognized on CT than with thoracic radiographs.