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Comparative Analysis of Dissimilatory Sulfite Reductase (dsr) Gene from Sediment of Lake Sihwa, Korea and Lake Aha, China (한국 시화호와 중국 Aha호 저질토에 분포하는 이화성 아황산염 환원효소 유전자의 비교 분석)

  • Kim, In-Seon;Kim, Ok-Sun;Jeon, Sun-Ok;Witzel, Karl-Paul;Ahn, Tae-Seok
    • Korean Journal of Microbiology
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    • v.44 no.2
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    • pp.147-155
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    • 2008
  • The diversity of sulfate reducing bacteria was investigated in different depths of sediments in Lake Sihwa, Korea and Lake Aha, China by PCR amplification, denaturing gradient gel electrophoresis (DGGE) and clone libraries targeting dissimilatory sulfite redectase (dsr) gene. In the analysis of DGGE band patterns, the community compositions of dsr gene in the sediments of both lakes were significantly different whereas bands in all depths of each environment revealed similar patterns. Bands from Lake Sihwa were produced much more than those from Lake Aha, demonstrating a higher diversity of dsr gene in Lake Sihwa. Total 68 clones containing dsr gene were obtained to analyze their sequences. Sequences from the sediment of Lake Sihwa were affiliated to Deltaproteobacteria, the Gram-positive thermophilic sulfate reducers belonging to the genus Desulforomaculum and archaeal thermophilic SRB belonging to the genus Archaeoglobus, whereas sequences from the sediments of Lake Aha were related to genus Desulfotomaculum. Clones retrieved from sediment of Lake Sihwa revealed a higher numbers than those of Lake Aha, demonstrating a higher diversity of dsr gene in Lake Sihwa. Most of clones (59%) were distantly related to the known cultivated SRB with $60\sim65%$ of similarity, which were clustered only the sequences from the environments showed less than 90% similarity. These habitat specific sequences suggested that the clustered dsr sequences represent species or groups of species that were indigenous to these environments. This study showed that these lakes have a specific bacterial communities having dsr gene distinct from those in other environments such as soil and marine ecosystems around the world.

Comparison of Pigments and Estimation of Production Period in Old and New Celestial Charts Folding Screens (신구법천문도 채색 안료 비교 및 제작시기 추정)

  • Oh, Joon Suk;Hwang, Min Young;Yamato, Asuka;Arai, Kei;Lee, Sae Rom
    • Journal of Conservation Science
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    • v.36 no.5
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    • pp.351-367
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    • 2020
  • The pigments of three old and new celestial charts folding screens(『Celestial Chart(Folding Screen)』 and 『Old and New Celestial Charts, Eight-Panel Folding Screen』 of National Folk Museum of Korea and 『Koudou-Nanboku-Ryousouseizu』 of National Diet Library of Japan) were analyzed to estimate their dating. It was estimated that the 『Celestial Charts(Folding Screen)』 was painted using traditional pigments from the Joseon dynasty such as azurite, indigo lake, malachite, atacamite, vermilion, iron oxide, cochineal, gamboge, orpiment, lead white, talc and soot. The green and blue colors of the 『Old and New Celestial Charts, Eight-Panel Folding Screen』 and 『Koudou-Nanboku-Ryousouseizu』 were painted using artificial inorganic pigments such as emerald green and ultramarine blue. These pigments were imported from Europe post the mid-19th century. In the 『Old and New Celestial Chart, Eight-Panel Folding Screen』, only artificial inorganic pigments were used for green and blue colors. However in the 『Koudou-Nanboku-Ryousouseizu』, emerald green and atacamite in green color, and ultramarine blue and indigo lake in blue color were used together. Based on both the results of pigment analysis and the study of star charts and inscriptions, the 『Celestial Charts(Folding Screen)』 was painted post mid-18th century. The 『Koudou-Nanboku-Ryousouseizu』 and 『Old and New Celestial Charts, Eight-Panel Folding Screen』 were painted after green and blue artificial pigments were imported in the mid-19th century. The 『Koudou-Nanboku-Ryousouseizu』 in which both traditional and western artificial pigments were used, can be dated earlier than the 『Old and New Celestial Chart, Eight-Panel Folding Screen』.

Study on Laboratory Diagnosis of the Ebola Virus and Its Current Trends (에볼라 바이러스 진단법과 개발 동향에 관한 고찰 연구)

  • Jeong, Hye Seon;Kang, Yun-Jung
    • Korean Journal of Clinical Laboratory Science
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    • v.47 no.3
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    • pp.105-111
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    • 2015
  • In late December 2013, the Ebola virus emerged from West Africa. The outbreak started in Guinea and rapidly spread to Liberia and Sierra Leone. Initially, the virus is spread to the human population after contact with infected wildlife and then spread person-to-person through direct contact with body fluids such as blood, sweat, urine, semen, and breast milk. The Ebola virus infects endothelial cells, mononuclear phagocytes and hepatocytes. It causes massive damage to internal tissues and organs, such as blood vessels and the liver, and ultimately death. Most tests for the virus RNA rely on a technology called reverse-transcriptase polymerase chain reaction (RT-PCR). While this method is highly sensitive, it is also expensive, requiring skilled scientists, and delicate power supplies. The strip analytical technique (enzyme-linked immunosorbent assay or ELISA) detects antigens or antibodies to the Ebola virus. This test is cheap and does not require electricity or refrigeration. Despite ongoing efforts directed at experimental treatments and vaccine development, current medical work on the Ebola viral disease is largely limited to supportive therapy. Thus, rapid and reliable diagnoses of the Ebola virus are critically important for patient management, infections, prevention, and control measures.

A Hierarchical Bitmap-based Spatial Index for Efficient Spatial Query Processing on Air (무선환경에서 효과적인 공간질의 처리를 위한 계층적 비트맵 기반 공간 색인)

  • Song, Doo-Hee;Park, Kwang-Jin
    • Journal of Internet Computing and Services
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    • v.12 no.6
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    • pp.43-51
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    • 2011
  • The recent development of the technology for wireless mobile computing and applied technology for location-based services has made it possible to support query processing faster than that in the past. However, some technological limitations on hardware and software still exist. The most critical aspects of query processing are accuracy and speed. For improving the accuracy, it is required that detailed information on the data involved in query processing be saved. In this case, the amount of information on the data increases, which decreases the speed. On the other hand, for increasing the speed, it is necessary to reduce the broadcast cycle, which enables rapid data acquisition as desired. In this case, because of insufficient index information, the listen time for the client increases, which may cause unnecessary energy consumption. Therefore, a trade-off occurs between the accuracy and speed. This paper proposes a hierarchical bitmap-based spatial index (HBI) as a solution for the aforementioned problems. HBI describes an object with 0 and 1 on the Hilbert curve map. It reduces the broadcast cycle by decreasing the index size on the basis of bit information and tree structure. Therefore, it is able to shorten the listen time and query processing time. In addition, HBI enables the detection of the locations of all the objects so that it is possible selectively listen to a broadcast. A performance evaluation of the proposed technique demonstrates that it is excellent.

Research Trends of Papers Published in Journal of the Korean Society of Grassland Science During Last Twenty (지난 20년간 한국 초지학회지에 발표된 논문의 연구동향)

  • 임용우
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.22 no.3
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    • pp.195-200
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    • 2002
  • Research trends of papers published in Journal of the Korean Society of Grassland Science(JKSGS) during last 20 years(1982~2001) were examined. Total number of papers published in JKSGS during last 20 years was 668. In the classification of published papers according to research objects, the rate of paper related to grassland was highest as average 18.6% of total research papers during last 20 years. Next was an orchardgrass 8.7, com 8.4, and sorghum and sorghum $\times$ sudangrass hybrid 7.1%. In classification of published papers according to research fields, cultivation field was studied more than others and the rate of cultivation field was highest as 38.8%, and physiology, utilization, breeding and biotechnology field was 23.7, 20.7, 9.1 and 6.6%, respectively. In comparison of average rate of research contents on the papers published during last 20 years, studies on the manure and fertilization were continously increased up to average 20% during recent 5 years(1997~2001). Study on the biotechnolgy was rapidly increased from average 6.6% of last 20 years to average 15% of recent 5 years. Study on the weed was also increased from average 2.5% to 5.9%. On the other hand, studies on the productivity and quality were rapidly decreased from average 31.3% of 5 years(1982~1956) to average 7.7% of recent 5 years. Research trend on the pasture appeared to be similar.

Comparison of Growth Characteristics, Productivity and Feed Values between Varieties of Sudangrass and Sorghum-Sudangrass Hybrids (수단그라스 교잡종과 수수-수단그라스 교잡종 간의 생육특성, 수량성 및 사료가치 비교)

  • Choi, Gi Jun;Lee, Sang-Hoon;Lee, Ki-won;Ji, Hee Chung;Hwang, Tae-Young;Kim, Ki-Yong
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.37 no.1
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    • pp.92-99
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    • 2017
  • This experiment was carried out to compare the growth characteristics and productivity between sudangrass and sorghum-sudangrass hybrid under the same cultivation condition in Cheonan and Kimje of Korea from 2010 to 2011. Sudangrass hybrids(Choice, Sunstar and Piper) and sorghum-sudangrass hybrids(SX-17, Jumbo and Revolution) were planted $5^{th}$ May in Cheonan and $7^{th}$ May in Kimje. Heading date of sudangrass hybrids was $12^{th}$ July which was about 2 week fast more than that of sorghum-sudangrass hybrids. Stem diameter of sudangrass hybrids was about 6mm which was about 5 mm smaller than that of sorghum-sudangrass hybrids. Disease resistance of sudangrass hybrid, "Choice" was similar to that of sorghum-sudangrass hybrids. Dry matter yield of "Choice" was 15ton/ha which was 10% less than that for sorghum-sudangrass hybrids(SX-17). Feed value of "Choice" was similar or slightly lower than that of sorghum-sudangrass. For increasing the productivity in forage cropping systems all around year, sudangrass hybrid is recommended for prolong by the middle ten day of May in the harvesting time of italian ryegrass.

Construction of CORBA Object-Group Platform for Distributed Real-Time Service (분산 실시간 서비스를 위한 CORBA 객체그룹 플랫폼의 구축)

  • Kim, Myung-Hee;Joo, Su-Chong
    • Journal of KIISE:Computing Practices and Letters
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    • v.7 no.6
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    • pp.602-613
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    • 2001
  • Recently, the computing has developing in distributed object computing environment for supporting a programming paradigm of distributed application requiring interoperability between heterogeneous clients and servers. It involves the complex networking and the object-oriented technologies for various multimedia application service. In this paper, we construct the real-time object group platform for solving the difficulties of managements of distributed objects and the real-time constraints by requiring for real-time service supporting of applications in distributed computing environment. The existing researches are being tried to only improving the performance of systems by using real-time CORBA itself, or modifying the part of CORBA compliance. Hence, we design a new model of real-time object group platform that can support the real-time requirement without modifying the ORB. The structure of our real-time object group analyzed and defined the requirement about object management and real-time application service sides. And the role of the components of real-time object group is divided into 2 classes for reducing the side effect of interoperability between management and service. Also, we considered how to transparently express the parameters of real-time properties for clients and developers of server's service objects. If the expression of real-time parameters is transparent, then the developer can easily extend the real-time parameters simply and flexibly. Therefore, in this paper we defined the role of components of platform and described functions of each component and designed and then implemented the real-time object group platform. Finally, we showed the execution procedures of implemented our platform for verifying the functionality.

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Experiment LOS Analysis of 3D Point Spatial Data (3차원 포인트 공간자료 가시선 분석 실험)

  • Park, Jae-Sun;Eo, Yang-Dam;Yeon, Sang-Ho;Moon, Jae-Heum;Kim, Hyung-Tae
    • Journal of Korean Society for Geospatial Information Science
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    • v.18 no.2
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    • pp.55-61
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    • 2010
  • Using 3D point data implemented from terrestrial LiDAR, this research has modelled geospatial data in 2 categories(gridded & un-gridded) and conducted LOS analysis experiment using outcome from the modeling exercise. To compare LOS analysis results from each of the 2 models in the above, maximum LOS (line of sight) range in the experimental area was specified as 30m in Area A, 40m in Area B and 50m in Area C and the time taken by LOS analysis and the number of visible points were measured. As for the LOS analysis experiment results, in comparison with the gridded model, the un-gridded model took about 3.9 times more time in Area A, 5.4 times in Area B and 6.5 times in Area C. In addition, about 0.97 times fewer points were measured in Area A, 0.93 times in Area B and 0.94 times in Area C. The difference between gridded model and un-gridded model in terms of the time taken by LOS analysis increased, as the maximum LOS range extended. On the other hand, the number visible points did not vary significantly in reference to the size of visible range.

A Study on Wearing Conditions and Dissatisfaction With Current Motorcycle Wear in Korea -Focus on Men′s Motorcycle Jacket- (국내 모터사이클복의 착용실태 및 불만족도에 관한 연구 -남성용 모터사이클 쟈켓을 중심으로-)

  • Hei-Sun;Do, Wol-Hee
    • Journal of the Korean Society of Clothing and Textiles
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    • v.26 no.12
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    • pp.1765-1777
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    • 2002
  • 본 연구에서는 현재 국내의 서울시내에 거주하는 모터사이클을 타는 만20세 이상의 남성을 대상으로 모터사이클복에 대한 착용실태와 불만사항들을 조사하고 이를 연령별, 모터사이클 유형별로 비교 분석하여 착용감 및 안전성이 향상된 모터사이클복 개발을 위한 기초자료를 제시 하고자 하였다. 설문조사는 2002년 1월~3월까지 3개월간 총 400부를 배부한 후 회수하고, 이 중 262부를 분석자료로 사용하여 빈도 및 백분율, 평균 등의 기술 통계치를 얻어 전반적인 착용현황을 알아보고, 교차분석을 실시한 후 X$^2$-검증을 실시하여 연령별, 모터사이클 유형별, 운행경력 별로 통계적인 차이를 비교 분석하였다. 1. 모터사이클복의 착용실태 및 불만사항 전반에 관한 조사결과, 조사대상자는 20대~40대의 운행경력은 5년 이상인 전문 라이더들이 많았으며 모터사이클의 배기량은 400cc이상이 가장 높게 나타났고, 모터사이클 유형은 로드 바이크가 가장 높게 나타났고, 이용목적은 레져.취미용, 선호하는 모터사이클복 아이템별 소재는 원피스와 슬랙스의 경우에는 피혁소재가 쟈켓의 경우에는 텍스타일 소재가 높게 나타났다. 아이템별 소유는 쟈켓과 투피스가 높게 나타났고, 보호구가 필요하다고 생각되는 신체부위는 상의에서는 가슴과 팔꿈치, 하의에서는 무릎으로 나타났으며, 무릎보호대의 소유도가 가장 높게 나타났다. 모터사이클복 주된 착용목적은 '부상방지', 모터사이클복을 착용하지 않는 이유는 '움직이기가 불편해서' '가격이 비싸서' '여름철에 더워서'가 각각 높게 나타났다. 모터사이클복에 대한 불만족도에서 동작적합성과 사이즈에 대한 불만도가 높게 나타났고, 모터사이클복소재 및 내부 장착 보호장구의 경량화가 요구되었으며, 모터사이클 쟈켓의 치수부적합부위는 소매길이가 가장높게 나타났다. 2. 전체 응답자의 연령을 20대, 30대, 40대 이상으로 나누어 연령별 차이를 비교한 결과 각각의 문항에 대해서 모두 연령대별로 유의차가 인정되었다. 3. 모터사이클복 아이템별 선호소재 문항에서 원피스와 쟈켓에서 모터사이클 기종별 유의차가 나타났는데, 비즈니스 기종에 서는 텍스타일 소재를, 아메리칸크루져 기종에서는 피혁소재를 선호하였다. 로드바이크와 오프로드 기종에서는 원피스는 피혁소재를 선호하였고, 쟈켓은 텍스타일 소재를 선호하는 것으로 나타났다.

Effects of Slow Release Recombinant Porcine Somatotropin (rpST) Administration on Growth Performance and pST and IGF-1 of Blood in Finishing Pigs (지속형 유전자 재조합 pST(Recombinant Porcine Somatotropin; rpST) 투여가 비육돈의 성장, 혈중 pST 및 IGF-1 농도에 미치는 영향)

  • 김영화;문홍길;박준철;정현정;김인철;이상진;장병선;정정수;정일병
    • Journal of Animal Science and Technology
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    • v.48 no.5
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    • pp.663-670
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    • 2006
  • The present study was performed to investigate the effect of plant type recombinant porcine somatotropin (pST) adminstration on growth performance and blood profile of finishing pigs. Forty-eight Landrace barrows weighing 78 kg were employed for six week growth trial. Twelve barrows were allocated into various rpST types ; the control (CONT), the 4:1 type rpST (TRT 1), the 1:1 type rpST (TRT 2); and the 4:1 type rpST group(TRT 3) respectively. CONT group were not planted rpST. TRT 1 group was treated with rpST once a week for 6 weeks. Each dose contained the rpST equivalent to 100mg from initial to two weeks and 125mg from three weeks to finial week. TRT 2 and TRT 3 groups were planted rpST four times contained the rpST equivalent to 100mg during trial period from initial to two weeks, and 125 mg from three weeks, to four weeks, respectively. All pigs were fed a commercial feed containing 0.9% lysine ad lib. Daily gain increased by 19.4% in TRT 1(p<0.05) compared to the CONT. rpST improved feed/gain by 13.4~28.9% in all treatment groups(p<0.05). Back fat thickness of the all rpST treated groups were 23.3~29.2% thinner than that of the CONT. These results indicated that the rpST stimulated growth performance in finishing pigs and plant type rpST could be used as a growth stimulant for finishing pigs.