• 한국가스학회:학술대회논문집
• /
• 2007.04a
• /
• pp.37-41
• /
• 2007

• Journal of the Korean Wood Science and Technology
• /
• v.32 no.2
• /
• pp.58-64
• /
• 2004

For the utilization of chestnut inner bark as forest biomass, the diethyl ether solubles of hot water extract from chestnut inner bark was analyzed by HPLC. Each peak was identified by comparing with retention time of standard regents and their purity from obtained UV spectrum by RI detector. Identified 6 compounds were gallic acid, 3,5-dihydroxybenzoic acid, 2,4,6-trihydroxybenzoic acid and protocatecualdehyde as phenolic acids and aldehyde, and catechin and epicatechin as flavonoids.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.29 no.3
• /
• pp.513-517
• /
• 2000

상황(Phellinus linteus) 및 아가리쿠스(Agaricus blazei Murill) 버섯으로부터 얻어진 메탄올 추출물과 이들 용매분획물에 대하여 마우스의 골수세포를 이용한 소핵 실험을 행하여 MNNG에 대한 유전독성 억제효과를 실험 하였다. 상황버섯 메탄올 추출물은 MNNG에 의한 소핵생성에 대하여 10.6~75.6%의 억제효과를 나타내었다. 또한 각각의 분획물의 경우 80mg/kg에서 에틸 아세테이트, 디에틸에케르, 부탄올, 클로로포름 그리고 물층이 각각 81.3,78.1,75.6,72.4 그리고 63.4%의 유전독성 억제 효과를 나타내었다. 아가리쿠스버섯 메탄올 추출물의 유전 독성 억제효과에서 양성대조군에 비하여 80mg/kg 투여시 86.2%의 억제효과를 나타내었으며, 디엘티에테르, 에틸 아세테이트, 클로로포름, 부탄올 그리고 물층 분획물의 경우 80mg/kg 투여시 86.2,81.3,78.1,69.9 및 61.8%의 억제효과를 나타내었다. 이상의 연구 결과에서와 같이 상황버섯과 아가리쿠스버섯의 추출물과 분획물들은 높은 유전독성억제효과를 나타냄으로서 건강식품으로서의 개발과 고부가가치의 의약품으로서 개발가능성을 가진 대단히 유용한 버섯임을 알 수 있었고, 다음 단계의 실험으로 이러한 생리활성을 나타내는 부분만을 분리, 정제하여 추가적인 검색 및 활용방안에 대하여 충분한 연구가 이루어져야 한다고 사료된다.

• Microbiology and Biotechnology Letters
• /
• v.26 no.4
• /
• pp.331-337
• /
• 1998

The biological activities of both ethanol and water extracts from the fruiting body of E. applanata and E. applanata mycelium and the three fractions of ethanol extracts from E. applanata were compared. 91% of MCF7 cell growth was inhibited by adding 0.5 g/l of water extracts of E. applanata and 81% of MCF7 cell growth was inhibited by adding 0.5 g/l of diethyl ether and chloroform fractions. It was also showed that above 60% of Hep3B cell growth was inhibited by adding all samples including the fractions. The ethanol extracts of E. applanata mycelium showed 33.3% of cytotoxicity on normal liver cell, WRL68 in adding 0.5 g/l of the samples and 40% in adding 0.5 g/l of chloroform fractions. The result of anti-mutagenicity of all extracts and fractions including ethanol extracts of Phelinus linteus were showed that diethyl ether fractions were most effective than any other samples. Hypoglycemic activities of diethyl ether and chloroform fractions were the most effective which scores were above 75%. The enhancement of glutathione-S-transferase activity was increased above 2.3 times by adding 1.0 g/l ethanol extracts of E. applanata and diethyl ether, chloroform fractions. It can be concluded that both biological activities of the fruiting body and mycelium of E. applanata were almost equivalent.

• Journal of the Korean Wood Science and Technology
• /
• v.34 no.3
• /
• pp.64-72
• /
• 2006

Two compounds were isolated from the diethylether and ethyl acetate fraction of hot water extracts by TLC and column chromatography as dyeing components. The isolated compounds were characterized as H-dibenzoquinoline-2,10-diol,5,6,6a,7-tetrahydro-1,9-dimethoxy-6-methyl (compoundI) and lyoniresinol (compoundII) by NMR and Mass spectrophotometry. To investigate if the isolated compounds are involved in the dyeing process, HPLC analysis was used. The retention time of the components from dye used in the study and the dye decolorized from the dyed substrate, silk by ethyl acetate were compared to conform the identity of those compounds. The retention time of the components from the dye and decolorized solution were identical. As a results, those two compounds were considered as dye bound to the silk.

• Korean Journal of Food Science and Technology
• /
• v.31 no.1
• /
• pp.260-262
• /
• 1999

Abies koreana Wilson is a proper tree to Korea. We tested the antimicrobial activity of the lignans isolated from it. The lignans inhibited the growth of Klebsiella pneumoniae and Vibrio parahaemolyticus at the concentration of $10^3\;ppm\;and\;10^4\;ppm$. Especially, the compound V showed higher inhibition activity to Vibrio parahaemolyticus by 1.7 times compared with the control. The compound III showed activity of 36% of the control to Klebsiella pneumoniae. The compounds II and VI had the high growth inhibition activity of 48, 66% respectively to Vibrio parahaemolyticus.

• KSBB Journal
• /
• v.17 no.1
• /
• pp.44-48
• /
• 2002

An efficient method of extraction for carotenoids in Rhodotorula glutinis KCTC 7989 was developed. Major carotenoids produced were identified as torularhodin of 61.7%, $\beta$-carotene of 28.8%, and torulene of 9.5%. HCI treatment, as a pretreatment on cell, was necessary to carry out together with thermal treatment unlike DMSO pretreatment. The choice of solvent had an important effect on the composition of carotenoids extracted: benzene and chloroform were effective for the extraction of torularhodin, especially. However, diethyl ether was most effective for the extraction of total carotenoids. Freeze dried type cells showed high efficiency value for the extraction of carotenoids, in compared with dried and wet type cells.

• Journal of the Korean Applied Science and Technology
• /
• v.34 no.2
• /
• pp.210-216
• /
• 2017

Recovery of low level nitric acid and sulfuric acid ions, which were contained in wastewater of a wet scrubber for de-NOx and de-SOx from ship engines, was attempted as fertilizing materials. This study utilized a selective extraction method using four organic solvents to precipitate the solid salts of ammonium nitrate and ammonium sulfate. The IR analysis showed almost same composition of the extracted ammonium salts with a commercial product, and recovery rate of nitrogen and sulfuric ions was 89% and 80% respectively. It was found that the selectivity and solubility consequently could be the crucial factors to recover the low level ions from the waste scrubbing water.

• Journal of the Korean Wood Science and Technology
• /
• v.33 no.6 s.134
• /
• pp.71-78
• /
• 2005

For the utilization of chestnut inner bark as forest biomass, this studies have been carried out cleary investigated about phenolic compounds from the diethyl ether solubles and ethyl acetate solubles of hot water extract from chestnut inner bark. 3 compound were isolated and their structures were identified by spectroscopic methods. They were known compounds, 3,5-dihydroxybenzoic acid, 3,4,5-trihydrixybenzoic acid and catechin. Electron donating ability (EDA) of 3 compounds were investigated. Gallic acid and catechin were found to higher EDA than control, ascorbic acid and $\alpha$-tocopherol in 10 ppm, and especially gallic acid has very higher EDA, 21 times than ascorbic acid. This gallic acid of chemical structure were substituted with free phenolic hydroxyl groups at meta and para position. It was supposed that phenolic hydroxyl groups in chemical structure was a EDA factor.

• Journal of Digital Convergence
• /
• v.13 no.5
• /
• pp.357-363
• /
• 2015

This study investigated the antioxidant and anti-inflammatory effects of Galla Rhois (Rhus verniciflua) on LPS (lipopolysaccharide)-stimulated human osteoblastic cells (MG-63). The aim of this study was to evaluate the LPS induced nitric oxide (NO) production and antioxidant radical in human osteoblast-like MG-63 cells. Therefore, the present work indicate that Galla Rhois extracts may be an ideal candidate for further research into their use for dental caries prevention component as well as, natural plant-based products. This suggested that 80% methanol and hexane extracts of Galla Rhois were inhibited NO generation and 1,1-diphenyl-2-pirylhydrazyl (DPPH) radical. Therefore, 80% methanol and hexane extracts of Galla Rhois may be utilized as a good source of protection against inflammation and oxidative stress. This study is intended to pursue wellness convergence in quality of life in the excavations in natural ingredients to create a variety of oral products with fewer side effects, cheap oral products for the dental treatment.