• Title/Summary/Keyword: 도법가(道法家)

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Evaluation and Application of Algae Online Analyzer for Alarming Algal Bloom and Water Quality Management of Korean Reservoirs (조류발생 경보 및 호수 수질관리를 위한 엽록소 자동측정기의 적용 및 타당성 연구)

  • Hwang, Su-Ok;Han, Myung-Soo;Kim, Baik-Ho
    • Korean Journal of Ecology and Environment
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    • v.39 no.2 s.116
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    • pp.257-264
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    • 2006
  • In order to evaluate the application of Algae Online Analyzer (AOA), an instrument of automatic measurement of chlorophyll a concentration, was tested and compared with the acetone extraction method on the basis of microscopic counting of phytoplankton in field water (Paltang Reservoir). We simultaneously conducted AOA operation and extraction method with the same water sample, to compare both results of chlorophyll a measurement. Phytoplankton were enumerated by inverted microscope with the Sedgwick-Rafter chamber, and classified into the genus or species. According to the AOA measurement, the diatom most (83.6%) strongly contributed to the total chlorophyll a concentration, followed by chlorophyceae> cyanophyceae>cryptophyceae. Overall, the results of both AOA and extraction method showed a similar trend and significant correlation (r=0.87, n=302, p<0.001), however, there were some differences according to the season and species. In particular, the relationship between AOA Chl-a density of the diatom (r=0.73, p=0.010) and cyrptophyceae (.=0.83, p=0.00154) were siginificant, while chlorophyceae (r= -0.13) and cyanophyceae (r= -0.16) showed no clear relationship during the study period. Although we can not fully understand why there was difference between both mothods, AOA application for alarming algal bloom and water quality management during the algal bloom appears to be very relevant. However, the further study or technical upgrade of AOA measurement is required, especially in the case of low density of phytoplankton or species-specific measurement.

The Effects of Charge Transfer Complex on the Reaction of N,N-dimethylaniline and Iodine (N,N-Dimethylaniline과 Iodine간의 반응에 있어서 Charge Transfer Complex의 영향)

  • Oh-Yun Kwon;U-Hyon Paek;Eung-Ryul Kim
    • Journal of the Korean Chemical Society
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    • v.36 no.2
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    • pp.191-196
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    • 1992
  • Reaction of N,N-dimethylaniline(N,N-DMA) and iodine in $CHCl_3,\;CH_2Cl_2 : CHCl_3$(1:1), $CH_2Cl_2$(1:1), and CH2Cl2 has been studied kinetically by using conductivity method. Pseudo first-order rate constants ($k_{obs}$) and second-order rate constants ($k_{obs}$/[N,N-DMA]) are dependent on the N,N-DMA concentration. Second-order rate constants obtained were decreased with increasing N,N-DMA concentration. We analysed these results on the basis of formation of charge transfer complex as a reaction intermediate. From the construction of reaction scheme and activation parameters for the formation and transformation of charge transfer complex. The equilibrium constants decreased when the dielectric constant of solvent was increased, and the value is 1.9${\sim}$4.2$M^{-1}$. The rate of transformation are markedly affected by the solvent polarity.${\Delta}H^{\neq}$ is 6.3-12.6kJ/mol, and ${\Delta}S^{\neq}$ is large negative value of -234J/mol K.

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Analytical Studies of $H_2O_2$-Producing Oxidase Systems ($H_2O_2$-생성 산화효소계에 관한 분석 연구)

  • Younghee Hahn;Hae-Lim Cho
    • Journal of the Korean Chemical Society
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    • v.37 no.10
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    • pp.874-880
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    • 1993
  • Spectrophotometric enzymatic analysis and amperometric enzymatic analysis for the determinations of glucose and ethanol were studied utilizing glucose oxidase (GO) and alcohol oxidase (AO), respectively, which commonly consume $O_2$ and produce $H_2O_2$. For the determination of glucose, $H_2O_2$ were coupled to $K_4Fe(CN)_6$ via peroxidase producing $K_3Fe(CN)_6$ whose absorbance was measured at 418 nm or whose diffusion current was measured on the glassy carbon electrode at an applied potential of -55 mV vs. Ag/AgCl (sat. KCl) reference electrode. Amperometric analysis was 1000 times more sensitive as well as 10 times better in the linear concentration range than spectrophotometric analysis. For the determination of ethanol, AO only was used for the enzymatic analysis, since $K_3Fe(CN)_6$ was completely disappeared as soon as AO was added. Either rate of $H_2O_2$ produced was amperometrically measured at +0.900 V or rate of $O_2$ consumed was measured at -0.500 V vs. Ag/AgCl(sat. KCl) reference electrode.

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Organic Precipitate Flotation of Trace Metallic Elements with Ammonium Pyrrolidinedithiocarbamate(Ⅰ). Determination of Bismuth, Cadmium, Cobalt and Lead in Water Samples by Coprecipitation-Flotation with Cu-pyrrolidinedithiocarbamate (Ammonium Pyrrolidinedithiocarbamate에 의한 극미량 금속원소의 유기침전 부선에 관한 연구(제1보) Cu-pyrrolidinedithiocarbamate 공침부선에 의한 물시료중 비스무트, 카드뮴, 코발트 및 납의 정량)

  • Jung, Yong June;Choi, Jong Moon;Choi, Hee Seon;Kim, Young Sang
    • Journal of the Korean Chemical Society
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    • v.40 no.12
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    • pp.724-732
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    • 1996
  • The organic precipitate flotation using Cu(II)-pyrrolidinedithiocarbamate complex as a coprecipitant was studied for the preconcentration and determination of trace Cd, Pb, Bi and Co in several water samples. Experimental conditions such as pH of solution, amounts of Cu(II) and ammonium pyrrolidinedithiocarbamate(APDC), stirring time, the type and amount of surfactant, etc. were optimized for the effective flotation of analytes. After 3.0 mL of 1,000 ${\mu}g/mL$ Cu(II) solution was added to 1.00 L water sample, the pH of the solution was adjusted to 2.5 with HNO3 solution. Trace amounts of analytes were coprecipitated by adding 2.0% APDC solution. And the precipitates were flotated onto the surface of solution with the aid of nitrogen gas and sodium lauryl sulfate. The floats were collected from mother liquor, and filtered through the micropore glass filter by suction. The precipitates were dissolved with 4 mL conc. HNO3, and then diluted to 25.00 mL with deionized water. The analytes were determined by graphite furnace atomic absorption spectrophotometry. This flotation technique was applied to the analysis of some water samples, and the 90 to 120% of recoveries were obtained from the spiked samples, this procedure could be concluded to be simple and applicable for the trace element analysis in various kinds of water.

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Oxygen Ring Formation Reaction of Mono-Oxo-Bridged Binuclear Molybdenum(V) Complex (II). Reaction of $[Mo_2O_3(Phen)_2(NCS)_4]$ with Solvent Water in Water + Co-Solvent Mixtures (한개의 산소다리를 가진 몰리브덴(V) 착물의 산소고리화 반응 (II). 2성분 혼합용매에서 용매물과 $[Mo_2O_3(Phen)_2(NCS)_4]$의 반응)

  • Sang-Oh Oh;Huee-Young Seok
    • Journal of the Korean Chemical Society
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    • v.32 no.3
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    • pp.203-210
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    • 1988
  • Mono-oxo-bridged binuclear molybdenum(V) complex, $[Mo_2O_3(Phen)_2(NCS)_4]$ produces di-oxo-bridged binuclear molybdenum(V) complex, $[Mo_2O_4(Phen)_2(NCS)_2]$ in water + co-solvent, where the co-solvent are acetone, acetonitrile and N,N-dimethylformamide. The rate of conversion of $[Mo_2O_3(Phen)_2(NCS)_4]\;into\;[Mo_2O_4(Phen)_2(NCS)_2]$ has been measured by spectrophotometric method. Temperature was $10^{\circ}C$ to $40^{\circ}C$ and pressure was varied up to 1500 bar. The rate constants are increased with increasing water mole fraction and decreased with increasing concentration of hydrogen ion. The order of oxygen ring formation reaction rate in various cosolvent is as follows, ACT > AN > DMF which is agreed with solvent dielectric constants. The observed negative activation entropy ($[\Delta}S^{\neq}$), activation volume($[\Delta}V^{\neq}$) and activation compressibility coefficient(${\Delta}{\beta}^{\neq}$) values show that the solvent water molecule is strongly attracted to the complex at transition state. From these results, the oxygen ring formation reaction of $[Mo_2O_3(Phen)_2(NCS)_4]$ is believed association mechanism.

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Pressure Dependence on the Aquation of s-cis-[Co(ee)$Cl_2$]+ and s-cis-[Co(eee)$Br_2$]+ ions (s-cis-$[Co(eee)Cl_2$]^+ 및 s-cis-$[Co(eee)Br_2]^+ $착이온의 수화반응에 미치는 압력의 영향)

  • Jong-Jae Chung;Sung-Oh Bek
    • Journal of the Korean Chemical Society
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    • v.32 no.4
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    • pp.318-322
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    • 1988
  • We studied the aquation reaction of s-cis-$[Co(eee)Cl_2]^+$ and s-cis-$[Co(eee)Br_2]^+$ complex ions under the various temperatures and pressures. In these complexes eee is $NH_2-CH_2CH_2-S-CH_2CH_2-NH_2$. The rate law of the aquation reactions of these two complexes obeys $Rate = k_{obsd}$[CO(III)], where rate constants of s-cis-$Co(eee)Cl_2]^+$ and s-cis-$[Co(eee)Br_2]^+$ respectively are $0.687{\times}10^{-4}$ $sec^{-1}$ and $4.10{\times}10^{-4}$ $sec^{-1}$ in condition of 0.1M $HClO_4\;and\;40^{\circ}C$. In the same condition, the activation entropies of s-cis-[Co(eee)$Cl_2$]+ and s-cis-(Co(eee)Br_2$]+ complexes respectively are -15.5 eu and -7.54eu, and the activation volumes are $-4.6cm^3mole^{-1}$ and $-4.2cm^3mole^{-1}$. From these data, we could infer the mechanism of the aquation reaction as the interchange dissociation (Id) mechanism.

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Community Structure of Pinus thunbergii Stand in the Eastern Coast of Gyeongsangbuk-do (경상북도 동해안 곰솔림의 군집구조)

  • Cheon, Kwang-Il;Jung, Sung-Cheol;Youn, Ho-Joong;Byeon, Jun-Gi;Joo, Sung-Hyun
    • Journal of agriculture & life science
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    • v.46 no.4
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    • pp.57-68
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    • 2012
  • This study was conducted to provide basic data for the effective conservation and management in the black pine(Pinus thunbergii) stand which is located in the eastern coast of Gyeongsangbuk-do where the fragmentation of vegetation has been caused by the exploitation and the increase of tourists, and installed the seventy study sites($20{\times}20m$) in the dominant black pine stand. The black pine stand was classified into three groups(P. thunbergii - P. densiflora community, P. thunbergii - Robinia pseudoacacia community, P. thunbergii - P. densiflora-Celtis sinensis community) by a cluster analysis. As a result of Multi-Response Permutation Procedures test, there is significance among the communities. Pinus densiflora, Callicarpa japonica, Juniperus rigida, Rhododendron yedoense for. poukhanense etc. nineteen species were significant by indicator species analysis. The population structure of black pine stand showed that the class of diameter 12 to 26 cm was dominance, which had the inverse hump-shape pattern. Species diversity index(H') of investigated ranged from $1.033{\pm}0.234$ to $1.629{\pm}0.226$ in the woody layer group and from $2.448{\pm}0.457$ to $2.545{\pm}0.318$ $2.174{\pm}0.333$ in the herb layer group.

Analysis of Rainfall-Runoff Characteristics in Gokgyochun Basin Using a Runoff Model (유출모형을 이용한 곡교천 유역의 강우-유출 특성 분석)

  • Hwan, Byungl-Ki;Cho, Yong-Soo;Yang, Seung-Bin
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.20 no.2
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    • pp.404-411
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    • 2019
  • In this study, the HEC-HMS was applied to determine rainfall-runoff processes for the Gokgyuchun basin. Several sub-basins have large-scale reservoirs for agricultural needs and they store large amounts of initial runoff. Three infiltration methods were implemented to reflect the effect of initial loss by reservoirs: 'SCS-CN'(Scheme I), 'SCS-CN' with simple surface method(Scheme II), and 'Initial and Constant rate'(Scheme III). Modeling processes include incorporating three different methods for loss due to infiltration, Clark's UH model for transformation, exponential recession model for baseflow, and Muskingum model for channel routing. The parameters were calibrated using an optimization technique with trial and error method. Performance measures, such as NSE, RAR, and PBIAS, were adopted to aid in the calibration processes. The model performance for those methods was evaluated at Gangcheong station, which is the outlet of study site. Good accuracy in predicting runoff volume and peak flow, and peak time was obtained using the Scheme II and III, considering the initial loss, whereas Scheme I showed low reliability for storms. Scheme III did not show good matches between observed and simulated values for storms with multi peaks. Conclusively, Scheme II provided better results for both single and multi-peak storms. The results of this study can provide a useful tool for decision makers to determine master plans for regional flood control management.

Anti-bacterial Activity and Standardization on Hwangryunhaedok-tang (황련해독탕의 항균활성 및 표준화 연구)

  • Lee, Dae-Yeon;Yi, Young-Woo;Kim, Wan-Su;Sim, Sun-Hyung;Kwon, Pil-Seung;Lee, In-Hee
    • Korean Journal of Clinical Laboratory Science
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    • v.51 no.2
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    • pp.214-220
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    • 2019
  • Hwangryunhaedok-tang (HT) is prescribed in oriental medicine to treat inflammation-related diseases. This study examined the anti-bacterial effect to select the index components of Traditional Chinese Medicine (TCM) HT. The disc diffusion method and minimum inhibition concentrations (MIC) tests were conducted to measure the antibacterial activity of HT. The strains were Staphylococcus aureus ATCC 25923 and Escherichia coli 23724. Berberine and geniposide were selected as the index components of HT and were analyzed quantitatively by high-performance liquid chromatography (HPLC). As a result, the inhibition ring was formed at $11.7{\pm}1.3mm$ and $8.7{\pm}0.7mm$ at HT 20 mg and 10 mg on S.aureus ATCC 25923, respectively; and $8.0{\pm}0.0mm$ at HT 20 mg on E.coli ATCC 23724. The MIC were 31.25 mg/mL in S.aureus ATCC 25923 and 125.0 mg/mL in E.coli ATCC 23724. In quantitative analysis for standardization, HT contained 16.55 mg/g of berberine and 81.85 mg/g of geniposide. Overall, when antibiotics are developed using natural products, they may reduce the side effects and inhibit resistant bacteria. If research on HT continuously proceeds, positive developments are expected to be made in oriental medicine as well as in various other industries.

A Study on Transition of Rice Culture Practices During Chosun Dynasty Through Old References IX. Intergrated Discussion on Rice (주요(主要) 고농서(古農書)를 통(通)한 조선시대(朝鮮時代)의 도작기술(稻作技術) 전개(展開) 과정(過程) 연구(硏究) - IX. 도작기술(稻作技術)에 대(對)한 종합고찰(綜合考察))

  • Guh, J.O.;Lee, S.K.;Lee, E.W.;Lee, H.S.
    • Korean Journal of Weed Science
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    • v.12 no.1
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    • pp.70-79
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    • 1992
  • From the beginning of the chosun dynasty, an agriculture-first policy was imposed by being written farming books, for instance, Nongsajiksul, matched with real conditions of local agriculture, which provided the grounds of new, intensive farming technologies. This farming book was the collection of good fanning technologies that were experienced in rural farm areas at that time. According to Nongsajiksul, rice culture systems were divided into "Musarmi"(Water-Seeded rice), /"Kunsarmi"(dry-seeded rice), /transplanted rice and mountainous rice (upland rice) culture. The characteristics of these rice cultures with high technologies were based of scientific weeding methods, improved fertilization, and cultivation works using cattle power and manpower tools systematically. Reclamation of coastal swampy and barren land was possible in virtue of fire cultivation farming(火耕) and a weeding tool called "Yoonmok"(輪木). Also, there was an improved hoe to do weeding works as well as thinning and heaping-up of soil at seeding stages of rice. Direct-seeded rice culture in flat paddy fields were expanded by constructing the irrigation reservoirs and ponds, and the valley paddy fields was reclaimed by constructing "Boh(洑)". These were possible due to weed control by irrigation waters, keeping soil fertility by inorganic fertilization during irrigation, and increased productivity of rice fields by supplying good physiological conditions for rice. Also, labor-saving culture of rice was feasible by transplanting but in national-wide, rice should not basically be transplanted because of the restriction of water use. Thus, direct-seeded rice in dry soils was established, in which rice was direct-seeded and grown in dry soils by seedling stages and was grown in flooded fields when rained, as in the book "Nongsajiksul". During the middle of the dynasty(AD 1495-1725), the excellent labor-saving farmings include check-rowing transplanting because of weeding efficiency and availability in rice("Hanjongrok"), and, nurserybed techniques (early transplanting of rice) were emphasized on the basis of rice transplanting ["Nongajibsung"]. The techniques for deep plowing with cattle powers and for putting more fertilizers were to improve the productivity of labor and land, The matters advanced in "Sanlimkyungje" more than in "Nongajibsung" were, development of "drybed of rice nursery stock", like "upland rice nursery" today, transplanting, establishment of "winter barly on drained paddy field, and improvement of labor and land-productivity in rice". This resulted in the community of large-scale farming by changing the pattern of small-farming into the production system of rice management. Woo-hayoung(1741-1812) in his book "Chonilrok" tried to reform from large-scale farmings into intensive farmings, of which as eminent view was to divide the land use into transplanting (paddy) and groove-seeding methods(dry field). Especially as insisted by Seo-yugo ("Sanlimkyungjeji"), the advantages of transplanting were curtailment of weeding labors, good growth of rice because of soil fertility of both nurserybed and paddy field, and newly active growth because rice plants were pulled out and replanted. Of course, there were reestimation of transplanting, limitation of two croppings a year, restriction of "paddy-upland alternation", and a ban for large-scale farming. At that period, Lee-jiyum had written on rice farming technologies in dry upland with consider of the land, water physiology of rice, and convenience for weeding, and it was a creative cropping system to secure the farm income most safely. As a integrated considerations, the followings must be introduced to practice the improved farming methods ; namely, improvement of farming tools, putting more fertilizers, introduction of cultural technologies more rational and efficient, management of labor power, improvement of cropping system to enhance use of irrigation water and land, introduction of new crops and new varieties.

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