Korean Journal of Ecology and Environment (생태와환경)

The Korean Society of Limnology (한국수생태학회)
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Evaluation and Application of Algae Online Analyzer for Alarming Algal Bloom and Water Quality Management of Korean Reservoirs

조류발생 경보 및 호수 수질관리를 위한 엽록소 자동측정기의 적용 및 타당성 연구

  • Hwang, Su-Ok (Pal-dang Office, Korean Water Resources Corporation) ;
  • Han, Myung-Soo (Pal-dang Office, Korean Water Resources Corporation) ;
  • Kim, Baik-Ho (Department of Life Science, Hanyang University)
  • 황수옥 (한국수자원공사 팔당권 관리단) ;
  • 한명수 (한국수자원공사 팔당권 관리단) ;
  • 김백호 (한양대학교 자연과학대학 생명과학과)
  • Published : 2006.06.30
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Abstract

In order to evaluate the application of Algae Online Analyzer (AOA), an instrument of automatic measurement of chlorophyll a concentration, was tested and compared with the acetone extraction method on the basis of microscopic counting of phytoplankton in field water (Paltang Reservoir). We simultaneously conducted AOA operation and extraction method with the same water sample, to compare both results of chlorophyll a measurement. Phytoplankton were enumerated by inverted microscope with the Sedgwick-Rafter chamber, and classified into the genus or species. According to the AOA measurement, the diatom most (83.6%) strongly contributed to the total chlorophyll a concentration, followed by chlorophyceae> cyanophyceae>cryptophyceae. Overall, the results of both AOA and extraction method showed a similar trend and significant correlation (r=0.87, n=302, p<0.001), however, there were some differences according to the season and species. In particular, the relationship between AOA Chl-a density of the diatom (r=0.73, p=0.010) and cyrptophyceae (.=0.83, p=0.00154) were siginificant, while chlorophyceae (r= -0.13) and cyanophyceae (r= -0.16) showed no clear relationship during the study period. Although we can not fully understand why there was difference between both mothods, AOA application for alarming algal bloom and water quality management during the algal bloom appears to be very relevant. However, the further study or technical upgrade of AOA measurement is required, especially in the case of low density of phytoplankton or species-specific measurement.

호수내 엽록소를 상시적으로 측정하기 위한 자동측정기(AOA)를 적용하고 타당성을 평가하기 위하여 서울-경기지역 상수원수인 팔당호 현장수가 직접 유입되는 제2취수장에서 시료를 채취하였다. 분석은 조류 현존량 및 종 조성 분석, 흡광광도법과 자동측정기에 의한 엽록소측정 등을 동시에 실시하여 그 결과를 비교 조사하고 현장 적용 가능성을 평가하였다. 연구결과. 조류분류군별 엽록소 기여도는 규조류가 83.6%로 가장 높았으며, 녹조류, 남조류, 크립토조류의 순으로 나타났다. 자동측정법과 흡광광도법에 의한 엽록소 농도는 전체적으로 매우 유의한 상관성을 보였으나(.=0.87, n=302, p<0.001), 시기 또는 공 조성에 따라 차이를 보였다. 자동측정기의 민감포는 크립토조 (r=0.83, p=0.00154)와 규조 (r=0.73,p=0.010)가 높았으며 현존량이 낮은 녹조(r=-0.13)와 남조(r=-0.16)에 대해서는 상관성을 보이지 않았다. 결과적으로 엽록소 자동측정법은 고전적인 아세톤 추출 방법에 비해 신속성과 경제성은 매우 인정되지만 출현종의 밀도나 종 천이 등을 고려한 조류분석 및 엽록소 직접측정 등이 병행되어야 할 것으로 사료되었다.

Keywords

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