• Title/Summary/Keyword: 대사체

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Establishment of rapid discrimination system of leguminous plants at metabolic level using FT-IR spectroscopy with multivariate analysis (FT-IR 스펙트럼 기반 다변량통계분석기법에 의한 두과작물의 대사체 수준 식별체계 확립)

  • Song, Seung-Yeob;Ha, Tae-Joung;Jang, Ki-Chang;Kim, In-Jung;Kim, Suk-Weon
    • Journal of Plant Biotechnology
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    • v.39 no.3
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    • pp.121-126
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    • 2012
  • To determine whether FT-IR spectroscopy combined with multivariate analysis for whole cell extracts can be used to discriminate major leguminous plant at metabolic level, seed extracts of six leguminous plants were subjected to Fourier transform infrared spectroscopy (FT-IR). FT-IR spectral data from seed extracts were analyzed by principal component analysis (PCA), partial least square discriminant analysis (PLS-DA) and hierarchical clustering analysis (HCA). The PCA could not fully discriminate six leguminous plants, however PLS-DA could successfully discriminate six leguminous plants. The hierarchical dendrogram based on PLS-DA separated the six leguminous plants into four branches. The first branch was consisted of all three Vigna species including Vigna radiata var. radiate, Vigna angularis var. angularis and Vigna unguiculata subsp. Unguiculata. Whereas Pisum sativum var. sativum, Glycine max L and Phaseolus vulgaris var. vulgaris were clustered into a separate branch respectively. The overall results showed that metabolic discrimination system were in accordance with known phylogenic taxonomy. Thus we suggested that the hierarchical dendrogram based on PLS-DA of FT-IR spectral data from seed extracts represented the most probable chemotaxonomical relationship between six leguminous plants.

Analysis of the Metabolites of 1,2,4-Trimethylbenzene by Capillary Electrophoresis (모세관 전기영동법을 이용한 1,2,4-트리메틸벤젠 대사체의 분석)

  • Kang, Jong-Seong;Hong, Cheong-Hee;Lim, Jeong-Mi;Lee, Yong-Moon;Jang, Jae-Yeon
    • Analytical Science and Technology
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    • v.12 no.4
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    • pp.326-331
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    • 1999
  • The metabolites of 1,2,4-trimethylbenzene (TMB) were synthesized and determined by capillary electrophoresis (CE). The optimum conditions of CE for the separation and determination of 3,4-, 2,4-, 2,5-dimethylbenzoic acid and 3,4-, 2,4-, 2,5-dimethylhippuric acid from the rat urine were as following: the fused silica capillary($75{\mu}m$ i.d. ${\times}$ 36 cm length, 29 cm to detector) was used and kept at $15^{\circ}C$. The applied voltage was 10㎸ and compounds were detected at UV 210 mnm and 254 nm. The running electrolyte was 0.1 M phosphate buffer (pH 7) containing 15 mM of ${\beta}-CD$ and 3% of 2-propanol. The relative amount of the metabolite of 1,2,4-TMB in the rat urine was 56.7% of 3,4-isomer, 30.5% of 2,4-isomer and 12.8% of 2,5-isomer. This method can be applied to the analysis of TMB-metabolites in human urine.

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Analysis of glucosinolates and their metabolites from napa cabbage (Brassica rapa subsp. Pekinensis) and napa cabbage kimchi using UPLC-MS/MS (UPLC-MS/MS를 이용한 배추와 배추김치의 글루코시놀레이트 및 대사체 분석)

  • Kim, Jaecheol;Park, Hyo Sun;Hwang, Keum Taek;Moon, BoKyung;Kim, Suna
    • Korean Journal of Food Science and Technology
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    • v.52 no.6
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    • pp.587-594
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    • 2020
  • In this study, we analyzed glucosinolates and their metabolites in the inner and outer parts of napa cabbage (NC; Brassica rapa subsp. pekinensis) and napa cabbage kimchi (NKC) using UPLC-ESI-MS/MS. In the extracts from NC and NKC, glucobrassicanapin (m/z 386), glucoalyssin (m/z 450), glucobrassicin (m/z 447), 4-methoxyglucobrassicin (m/z 477), and neoglucobrassicin (m/z 477) were detected using the MS scan mode ([M-H]-), and gluconapin (m/z 372→97), progoitrin (m/z 388→97), glucoiberin (m/z 422→97), 4-methoxyglucobrassicin (m/z 477→97), and neoglucobrassicin (m/z 477→447) were detected using the MS/MS MRM mode ([M-H]-). Ascorbigen (m/z 306→130) and indole-3-carboxaldehyde (I3A; m/z 146→118), which were metabolites of glucobrassicins, were detected using the MS/MS MRM ([M+H]+) mode. The peak intensities of ascorbigen in the extract from the inner and outer parts of NC were significantly higher than those of the NKC extract (p<0.05); however, there was no significant difference in I3A peak intensity between the NC and NKC extracts.

Physiological Function of Insulin-like Peptides in Insects (곤충 insulin-like peptide의 생리 조절 작용)

  • Kim, Doo Kyung;Lee, Jaemin
    • Korean journal of applied entomology
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    • v.61 no.1
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    • pp.85-90
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    • 2022
  • Insulin and insulin-like growth factor-1 (IGF-1) are hormones that play an important role in the physiological regulation of metabolism, growth, and longevity in vertebrates. Likewise, insulin-like peptides (ILPs), which are structurally similar to insulin and IGF-1, are crucial in insect physiology. In this review, we present an integrated summary of insect ILPs and their receptor signaling, which has been shown to be comparable to insulin and IGF-1 receptor signaling in vertebrates based on genetic studies of Drosophila melanogaster. Additionally, we review the control of ILP synthesis and secretion in the brain in response to nutrition, as well as the ILPs' physiological role in insect metabolism. Moreover, we discuss the contribution of ILPs to growth, development, reproduction, and diapause. Finally, we consider the possibility of targeting ILP receptor signaling in pest management.

신규항암제 DA-125의 약리.대사연구

  • 이상득;김원배;김순희;류병권;고광호;이봉진;서동욱;배경아;공재명
    • Proceedings of the Korean Society of Applied Pharmacology
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    • 1994.04a
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    • pp.310-310
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    • 1994
  • 1. 항암력 평가 : 마우스 고형종양 M5076, Colon26, Colon38에 대하여 DA-125는 DXR보다 높은 종양억제율과 많은 완치예를 보였다. 2. 일반약리작용 : M1의 호흡순환기계 및 적출 평활근에 대하여 유의한 약리작용은 인정되지 않았다. 3. 대사 및 대사효소유도작용 : DA-125를 랫드에 14일간 반복투여시 약물대사효소유도작용을 나타내지 않았으며, 랫드에서의 DA-125의 주대사경로는 M1->M2->M4로 추정되었다. DA-125 4mg/kg을 랫드에 정맥투여시, 24시간동안 담즙으로 배설된 M1, M2, M3 및 M4의 양은 투여량의 25.3%에서 $\beta$-glucuronidase 처리후 39.5%로 증가하였으며, 효소처리후 포합체 형태로 배설되는 6개 이상의 형광을 갖는 미확인 대사체가 발견되었다.

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Synthesis of Rhamnazin and Ombuin as Methylated Metabolites of Quercetin (케르세틴의 메틸화된 대사체인 람나진과 옴부인의 합성)

  • Jang, Jongyun;Kang, Dong Wook
    • Journal of the Korean Chemical Society
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    • v.62 no.1
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    • pp.19-23
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    • 2018
  • The methylated metabolites of quercetin, rhamnazin and ombuin are highly likely to develop as anticancer and anti-inflammatory agents. In this study, we synthesized rhamnazin through selective methylation of quercetin hydroxyl group, which has not been reported so far. In addition, a new synthetic method was developed to correct the problems of previous synthetic method of ombuin, one of the methylated metabolites of quercetin.

Metabolic Adjustment of Lactate Dehydrogenase Isozymes to a Change in Dissolved Oxygen in Bluegill (Lepomis macrochirus) (파랑볼우럭(Lepomis macrochirus)에서 용존산소량의 변화에 대한 젖산탈수소효소 동위효소들의 대사조절)

  • Ku, Bora;Cho, Sung Kyu;Yum, Jung Joo
    • Journal of Life Science
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    • v.31 no.12
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    • pp.1066-1071
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    • 2021
  • The aim of this study was to examine the metabolic adjustment of lactate dehydrogenase (EC 1.1.1.27, LDH) isozymes to a change in dissolved oxygen (DO) in bluegill (Lepomis macrochirus). After bluegills were adapted to a constant environment in an aquarium, the DO was changed to investigate the activity of LDH isozyme and the relative ratio of subunits A, B, and C for each tissue. When the DO was decreased from 18 ppm to 6 ppm, LDH in skeletal muscle, heart, and brain tissues recovered to the level of control activity within 12, 12, and 6 hr, respectively. LDH activity changed in accordance with a change in DO. The compensation was performed rapidly and is thought to be an important function of LDH in enabling bluegills to adapt to their environment. In bluegill heart, eye, and brain tissues, the relative ratio of subunit A increased and showed a tendency to recover similarly to the subunit ratio of control groups up to 12 hr. It is thought that the anaerobic metabolism using subunit A was increased in the initial stage when DO was changed. In addition, the results revealed that subunit C was more similar to subunit A than subunit B. In bluegills, subunits A and C of LDH seem to be evolutionarily similar. LDH isozymes, mainly containing subunits A and C, are likely responsible for the function of pyruvate reductase, which plays a role in making the bluegill adapt to a hypoxic environment through anaerobic metabolism.