• Journal of Korean Society of Forest Science
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• v.97 no.6
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• pp.650-655
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• 2008

In an attempt to establish the mass proliferation system, Eucalyptus pellita, a 5-year-old plus tree, was cultured with three different culture types in 1L vessels: solid culture without ventilation (conventional culture), liquid culture without ventilation and open-type liquid culture with forced ventilation. Then the culture scale was subsequently increased from 1L to 10L in vessel volume. After 4 weeks of 1L-scale culture, the best growth was obtained by culturing plantlets on open-type liquid culture, suggesting that the in vitro plantlets growth can be enhanced by liquid medium and ventilation. In open-type large scale culture in 10L vessel, plantlets growth resulted in a 370% increase in the number of nodes, 3.6 times increase in leaf expansion, and 3.3 times increase in shoot length, while the conventional culture suppressed shoot growth due to the callusing on the leaves and lack of $CO_2$. The results indicated that the open-type large scale culture system was effective for enhancing productivity by improving growth of the plantlets in clonally proliferated plus tree, Eucalyptus pellita.

• Proceedings of the Korean Society for Agricultural Machinery Conference
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• 2002.02a
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• pp.401-406
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• 2002

식물조직 배양공정의 기계화 기술개발을 위한 기초자료를 얻고자 식물조직 배양기술의 개발 또는 식물묘를 대량생산하는 연구지도기관 및 농원 등의 배양실 10개소를 대상으로 배지의 분주, 배양용기의 밀봉 및 세척, 배양체의 접종공정 등에 대한 작업실태를 조사분석한 결과를 요약하면 다음과 같다. 가. 배양기술의 개발 또는 식물묘 대량생산은 대부분이 나리, 호접란, 풍란, 심비디움 등 화훼류였으며, 배지는 모두 한천이 혼합된 고체배지, 배양용기는 형상 및 용량 등이 다른 플라스크, 배양병, pE용기 등이 사용되었다. 나. 배지의 분주작업은 조사대상 10개배양실중 6개소는 작업자가 비이커를 사용하여 분주하였고 4개소는 자동 또는 반자동분주기를 사용하였으며, 인력 및 반자동분주기의 사용시 배양용기간의 분주량은 기준량의 $\pm$25%범위에서 차이가 있었다. 다. 액체배지의 분주시 온도는 36.8~88.$0^{\circ}C$, 점도는 3.7~11.8cP 범위였고 점도는 배지에 혼합하는 한천, 사과 등 유기물의 량에 따라 차이가 있으므로 배지의 분주작업을 기계화하기 위해서는 배지의 온도와 점도, 배양용기와 분주량 등을 종합적으로 고려하여 배지의 점도에 따라 분주량을 조절할 수 있도록 개발할 필요성이 있다고 생각된다. 라. 배양용기의 밀봉소재는 플라스크의 경우 호일 또는 고무마개와 호일, 배양병은 모두 나사산이 있는 캡, PE용기는 뚜껑을 사용하였으며, 밀봉작업은 모든 배양실에서 인력에 의존하였고 작업능률은 호일만으로 밀봉하는 경우 시간당 180~240병, 캡은 시간당470~600개 정도였다. 마. 배양체의 접종작업은 모든 배양실이 인력에 의존하였으며, 배양체를 배지와 분리하여 불필요한 부분을 제거하고 배양작물에 따라 생육정도를 2~3등급으로 구분하여 배양용기의 배지 위에 치상하는 과정으로 수행되었으며, 작업능률은 호접란의 경우 배양병에 25본을 접종하는데 시간당 6병, 심비디움은 원형 플라스크에 25본을 접종하는데 시간당 10병 정도였다. 바. 식물체의 대량증식에 사용되는 플라스크, 배양병, PE용기 등 배양용기의 세척작업은 농원의 1개배양실에서 간이식 세척기, 이 외의 9개배양실은 모두 물에 담겨 두었다가 세제와 브러쉬 등을 사용하여 인력으로 세척하고 있어 생력화 기술개발이 요구되었다.

• Journal of Korean Society of Forest Science
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• v.100 no.2
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• pp.212-217
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• 2011

A photoautotrophic micropropagation methodology in liquid culture medium has a number of advantages for large-scale propagation of plants. This paper describes an improved system for the mass propagation via somatic embryogenesis of the medicinal plant Kalopanax septemlobus Nakai. Somatic embryo-derived young plantlets of K. septemlobus were cultured either under heterotrophic conditions with sucrose on half-strength MS medium with $30gL^{-1}$ sucrose, under heterotrophic conditions without sucrose, or under photoautotrophic conditions (MS liquid medium without sucrose under forced aeration) for four weeks before transferring the plantlets for acclimatization. Plantlets grown under photoautotrophic conditions had more leaves, higher chlorophyll content, a higher net photosynthetic rate (NPR), and a higher survival rate. The results indicate that the photoautotrophic conditions with a forced ventilation system are effective in enhancing the growth of plantlets and the rate of net photosynthesis. The plantlets grown under photoautotrophic conditions had a high survival rate (92%) upon ex vitro transplantation. Our study shows that autotrophically produced plantlets acclimatize better and sooner upon ex vitro transplantation than conventionally cultured plants.

• 한국생물공학회:학술대회논문집
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• 2000.11a
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• pp.254-255
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• 2000

형질전환된 인삼 모상근의 여러 생물반응기에서의 대량배양 가능성을 알아보기 위하여 1/2 MS 배지를 기본배지(호르몬 무첨가, 3% sucrose)로 하여 실험을 수행하였다. 그 결과 플라스크 배양에 비하여 $2{\sim}3.5$배 높은 성장률을 보였으며, 그 성장이 왕성함을 알 수 있었다. 배양 후반기에 모상근의 뭉치 형성으로 물질전달의 어려움으로 인하여 성장에 장해를 미치는 것으로 사려된다.

• Applied Biological Chemistry
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• v.50 no.1
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• pp.1-5
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• 2007

For the purpose of application for biomass of Pleurotus eryngii, the optimum culture condition were tested. It was found that the optimum culture condition for spot culture of pleurotus eryngii were 24$^{\circ}C$ for 18 days with PDA medium. And the optimum culture condition of bioreactor for biomass were pH 5.5, 18$^{\circ}C$ and 27 days with PDMP broth. It was possible to artificial cultivation of mycelial from Pleurotus eryngii using bioreactor for biomass under the optimum conditions, and it was also possible for Pleurotus eryngii biomass because the forming of fruiting body when Pleurotus eryngii was cultivated using mass artificial cultivated mycelial in the bioreactor.

• Journal of Sericultural and Entomological Science
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• v.38 no.2
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• pp.150-153
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• 1996

To develope a microbial pesticide for the control of agricultural and forestal pests in Korea, the mass culture system of entomopathogenic fungi was studied. Previously, we have developed the mass culture system which was adaptable for the culture of Beauveria bassiana. In this study, we determined the efficacy of this mass culture system for other entomopathogenic fungi, B. bassiana, Beauveria brongniartii, Metarhizium anisopliae, and Verticillium lecanni. To determine the efficacy of mass culture system, we examined the growth rate of entomopathogenic fungi in this system which was composed of 1st liquid media for growth of blastospore and 2nd pellet media for growth of conidia. As the result, we obtained that the blastopore numbers increased 103-104 times in liquid media at 72 hrs post inoculation. The results showed that this mass culture system for the growth of entomopathogenic fungi was effective.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2002.11b
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• pp.29-29
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• 2002

참오갈피(Acanthopanax pedunculus)는 국내 고유한 자생 종이며 현재 몇 그루 만이 존재하는 것으로 추정된다. 이 수종은 대체적으로 다른 오갈피 수종에 비해 잎 및 열매는 물론 식물 전체 부위가 다소 커서 경제성이 높다고 판단된다. 본 실험에서는 참오갈피의 세포배양 기술을 이용한 묘목 및 약용원료의 산업적 대량 생산 기술을 개발하고자 수행 하였다. 참오갈피 접합자배를 적출하여 무균적으로 배양한 다음 자엽단계로 성숙한 이들 접합자배를 1 mg/1 2,4-D가 첨가된 MS 배지에 배양하여 배형성 캘러스를 유도하였다. 배형성 세포의 유도율은 약 72%에 달했다. 유도된 배형성 캘러스는 2,4-D가 첨가된 같은 고체 및 액체 배지에서 계대배양하여 배형성 캘러스 및 세포를 유지하였다. 배형성 캘러스를 2,4-D가 첨가되지 않은 배지에 옮겨주면 이들 세포로부터 체세포배가 발생되었다. 약 2개월의 기간을 거쳐 자엽단계로 성숙된 체세포 배는 발아되지 않기 때문에 5 mg/1 GA$_3$가 첨가된 1/2 MS 배지에 옮겨 발아 및 식물체로 재생 시켰다. 약 7 cm 크기로 재생된 식물체를 인공토양에 옮겨 1달간 순화시키고 난 후 토양에 옮겼을 경우 87%가 생존하였다. 기내에서 유도된 참오갈피 식물체는 특별한 처리를 하여주지 않아도 뿌리로부터 배발생 세포를 형성 할 수 있었다. 따라서 이들 식물체로부터 배발생세포 유도는 세포주의 보존 및 증식에 효과적으로 이용될 수 있었다. 한편 참오갈피 세포는 5-10 리터 바이오리엑터 배양을 통하여 배형성세포, 자엽단계의 배, 유식물의 대량생산의 가능성을 확인하였다.

• Journal of Plant Biotechnology
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• v.35 no.1
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• pp.75-80
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• 2008

In vitro culture of shoot tip of garlic (Allium sativium L. cv. Seosan) was carried out to find medium condition of the induction of multiple shoots and bulbing for muliproduction of virus-free seed bulbs. For this work, tank culture was introduced. In shoot tip culture on MS solid medium the induction of multiple shoots and bulbing were better by adding 3% sucrose than 8%. Supplementation with 2mg/L 2ip and 0.2 mg/L IAA in this medium was effective. Three point three shoots including 2.7 bulbs were formed from a shoot tip after cultivation for 30 days on this medium. Bulbing of garlic in liquid culture with plastic water tank of 20L supplied air at the side of the lower part was better by adding 3% sucrose than 8% by subculture for 45 days with shoots obtained from shoot tip culture for 30 days on soid MS medium. Shoot growth was vigorous at 3% sucrose however bulb growth was more effective on the medium of 8% sucrose. Because of the effectiveness on solid medium added 3% sucrose, 2 mg/L 2ip and 0.2 mg/L IAA for initial production of multi-shoot in stem tip culture and the effectiveness in liquid culture with water tank for growth of bulbs, the method of two-step culture could be introduced for the multiple production of seed bulb of high quality. It was more desirable by supply of 0.2 mg/L BA and 0.02 mg/L NAA at tank culture time. But growth of the bulbs became poor by increasing concentration of NAA of the medium.

• Journal of Plant Biotechnology
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• v.30 no.1
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• pp.65-71
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• 2003

This research aims the production of anti-tumor substances through in vitro culture of hairy roots transformed by Agrobacterium rhizogenes in Artemisia sylvatica MAX and the effect of culture conditions on optimum growth of hairy roots. We investigated the optimum medium, pH, carbon source, sucrose, light, Fe and polyamine conditions of various lines of hairy roots (NK3, NK4, YX. NK3-10) induced from Artemisia sylvatica to increase the optimum growth of hairy roots. MS medium was the best for optimum growth of hairy root clone, NK3-S10. The optimum culture period was 4 weeks for NK3-S10. The optimum sucrose concentration was 3.5%. The optimum concentration of FeSO$_4$, spermine and spermidine was 0.1 mM, 10 mM and 100 mM, respectively.

• Horticultural Science & Technology
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• v.28 no.5
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• pp.845-849
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• 2010

This study was conducted to compare various culture methods and evaluate economic feasibility of each method for mass propagation of new ever-bearing strawberry 'Goha'. Four different methods such as semi-solid culture, solid culture, liquid suspension culture and bioreactor culture were compared. The solid culture and bioreactor culture showed the shortest and longest root length, such as 3.6 cm and 8.3 cm, respectively. Fresh weights of plants cultured in bioreactor were 2,261 mg, which were heavier than those of cultures. Dry weights of plants cultured in bioreactor were the heavier compared to those in other cultures. The number of axillary bud developed in bioreactor was seven, but axillary bud was not developed in other cultures. Production cost through bioreactor culture was calculated to be 303 won per plant which was 542 won less than that of solid culture. As a result, we found that the bioreactor culture was the most cost effective culture method for in vitro mass propagation in new ever-bearing strawberry 'Goha'.