• Korean Journal of Fisheries and Aquatic Sciences
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• v.25 no.1
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• pp.45-50
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• 1992

To utilize shellfish by-products effectively, chitin, chitosan, and microcrystalline chitin were prepared from 6 kinds of crustacean shells(Antarctic krill, Euphausia superba; Red snow crab, Chinonecetes japonicus: Daelongsuyum shrimp, Solenocera prominentis: Lobster, Linuparus trigonus: Gasibal shrimp, Nephrops thomsoni: Blue crab, Portunus trituberculatus) and their functional properties were studied. Apparent volume(AV), settling volume(SV), water binding capacity(WBC), and fat binding capacity(FBC) of various chitins, chitosans, and microcrystalline chitins ranged from $3.1\pm0.1ml/g\;to\;27.0\pm0.2ml/g$ from $5.1\pm0.1ml/g\;to\;45.0\pm0.2ml/g,\;from\;318\pm40g/100g\;to\;2,382\pm12g/100g,\;and\;from\;235\pm20g/100g\;to\;2,169\pm20g/100g$, respectively, and the krill chitin and chitosan had the highest AV, SV, WBC, and FBC of them. Chitins and chitosans did not produce emulsion but microcrystalline chitins showed emulsifying properties. Emulsifying capacity and stability of various microcrystalline chitins ranged from $18.2\pm4.0\%\;to\;50.1\pm2.5\%\;and\;from\;15.2\pm3.5\%\;to\;31.1\pm1.0\%$, respectively. Dye binding capacity of microcrystalline chitins was higher than that of chitins or chitosans.

• Clinical and Experimental Pediatrics
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• v.48 no.6
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• pp.669-674
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• 2005

Nephrogenic diabetes insipidus (NDI) is a disorder in which the secretion of antidiuretic hormone is normal, but the response of the renal collecting tubules to vasopressin is impaired. Compared with acquired NDI (a-NDI), which is secondary to chronic bilateral incomplete urinary tract obstruction with hydronephrosis, congenital NDI (c-NDI) is a very rare heritable disorder that usually follows the X- linked recessive pattern. Clinical symptoms of c-NDI can be non specific, and often the disease ultimately results in failure to thrive, or mental retardation. Recently, the diagnosis can be confirmed by direct sequencing analysis of the peripheral blood specimens. The long-term results of treatment for c-NDI are not satisfactory. Reports on the follow up of c-NDI cases are rare and there is no report on the cases treated with combinations of three drugs. We report herein a case of severe c-NDI in an 8 year-old-boy with a severely dysconfigurated urinary tract system. The patient and his mother showed a frameshift mutation on the AVPR2 gene on chromosome Xq28:.847_851delTGCTG (p.C283fsX90). The patient showed normal growth and development by treatment with combinations of hydrochlorothiazide ($65mg/m^2$), amiloride (0.3 mg/kg/d) and indomethacin ($100mg/m^2$), yet after five years he needed adjuvant cystostomy to relieve him from the residual symptoms of urgency with polyuria.

• Journal of Korean Ophthalmic Optics Society
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• v.5 no.1
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• pp.187-192
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• 2000

The objective of this study was to investigate the problems of soft contact lens wear on the women college students. The results were as follows 1. Almost the purchase of soft contact lens was the optical shop. 2. Almost the motive of soft contact lens use was the discomfort of glasses wear. 3. Almost the good feeling of glasses wear was moderate degree. 4. On the main reason of exchange of contact lens, teens was contact lens damage and tweenths was lifespan of contact lens. 5. On the contact lens wearing time at one day, teens was over 10 hours and tweenths was from 7 hours to 10 hours. 6. On the contact care and treatment, teens was a lens washing and storage and tweenths was a lens washing and disinfectant. 7. On the main difficult of contact lens handling, teens was a lens separation in eye and disinfection of lens case and tweenths was a solution treatment and insertion of contact lens in the eye. 8. On the contact lens insertion in the eye, the most difficult item was hanging of the eyelid and fear thinking in teens and fear thinking in tweenths. 9. On the difficult in separate of contact lens wear, a most subjects were eye opening. 10. On the most inconvenient state in contact lens wearing, the subjects were eye redness, foreign body and eye pain. 11. On the advantage of contact lens wearing, teens was decrease in glasses side effects and twenties was a beauty.

• Industry Promotion Research
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• v.2 no.1
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• pp.77-83
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• 2017

The purpose of this study is to analyze the effect of sustainability of festival in Gangwon province on satisfaction. A total of 243 questionnaires were analyzed for the tourists participating in the Gangwon - do area festival. Using SPSS Statistics 20, we conducted frequency analysis, factor analysis, reliability analysis, correlation analysis, regression analysis, and ANOVA (Variance Analysis of Variance among Respondents). The results of this study are as follows. First, the satisfaction of the local festival persistence in Gangwondo area festival was found to be higher when the service satisfaction, program satisfaction, and red general satisfaction were higher in the regional festival sustainability. And it was analyzed to have a statistically significant effect. Second, there was no statistically significant effect of participating motivation on the program festival. Third, the correlation between the persistence of local festivals and the satisfaction of the local specialties was higher in R = .812, and the t value was 21.569 (p = .000) Respectively. The purpose of this study was to examine the effect of personal factors on regional festival tourists' satisfaction with regional festival sustainability and participatory motivation for service satisfaction, program satisfaction, There is a significance of research.

• Clinical and Experimental Pediatrics
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• v.53 no.2
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• pp.262-266
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• 2010

Tumor necrosis $factor-{\alpha}$ ($TNF-{\alpha}$) is a major proinflammatory cytokine involved in the pathophysiology of juvenile rheumatoid arthritis. Etanercept is an effective inhibitor of $TNF-{\alpha}$ and has shown a beneficial effect in patients with JRA. However, the most important cause of concern related to etanercept administration is infection. We report a case of encephalitis in a JRA patient receiving long-term treatment with etanercept. The patient was a 4-year-old boy with refractory JRA, and he received etanercept subcutaneously at a dose of $0.4\;mg\;kg^{-1}\;day^{-1}$ twice a week for 14 months, along with non-steroidal anti-inflammatory drugs, methotrexate, oral steroids, and sulfasalazine. The patient presented with sudden fever, headache, vomiting, a generalized tonic seizure, and changes in mental status. We suspected a central nervous system infection, and simultaneously administered antibiotics, an antiviral agent, and steroids. After 2 days of hospitalization, his mental function returned to normal, and he showed no further seizure-like movements. Brain magnetic resonance imaging scan of the patient showed a multifocal cortical lesion on both sides of the temporoparietooccipital lobe, which indicated encephalitis. Although we were unable to identify the causative organism of encephalitis, we think that the encephalitis may be attributed to infection, and the use of etanercept may have increased the risk of severe infection. Therefore, etanercept was discontinued and the patient recovered shortly after. To the best of our knowledge, this is the first case of encephalitis in a juvenile rheumatoid arthritis patient treated with etanercept.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.18 no.12
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• pp.208-212
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• 2017

A urinary calculus in the urethra is rarely seen and usually encountered in men with a urethral stricture or diverticulum. Herein, we report a rare case of a giant calculus in the urethra of a 42-year-old male patient with paraplegia after spinal cord injury due to car accident 20 years ago. A recent urologic consultation from the emergency room was performed since the patient had multidisciplinary symptoms during the day without any urination and presented with urinary difficulties due to hematuria and pain symptoms occurring with a mass in the bottom of the testicles. Abdomen and pelvic computed tomography (CT) was performed to examine the tumor mass, which was found to be absent. In addition, urethral stones were observed in the CT. Cystostomy was performed after the first urethroplasty, and the stone was removed from the urethra. Two weeks later, the patient was subjected to urethrography to remove the Foley catheter. No specific finding in voiding was detected. Giant urethral stones sometime require differentiation from tumor status. Treatment may vary according to size and location, requiring careful examination.

• Journal of Life Science
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• v.18 no.12
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• pp.1686-1692
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• 2008

This study was performed to investigate the effects of the chitosan oligosaccharide administration on the improvement of the lipid compositions, blood glucose and enzyme activities in the serum of hypercholesterolemic rats fed the experimental diets for 5 weeks. Concentrations of total cholesterol, atherosclerotic index, LDL, LDL-cholesterol, free cholesterol, cholesteryl ester ratio, triglyceride (TG), phospholipid (PL) and blood glucose in serum were higher in the hypercholesterolemic group (group CW) and cholesterol+chitosan oligosaccharide administration group (group CCW) than those in the control group (group BW, basal diet+water). But the concentrations of total cholesterol, atherosclerotic index, LDL, LDL-cholesterol, free cholesterol, cholesteryl ester ratio, TG, PL and blood glucose in serum were lower in the group CCW than those in the CW, whereas the ratio of HDL-cholesterol concentration to total cholesterol and HDL-cholesterol concentration in the group CCW were higher than in the group CW. The activities of alkaline phosphatase (ALP) and aminotransferase (AST, ALT) in serum were lower in the group CCW than in the hypercholesterolemic group CW. From the above results, it was suggested that chitosan oligosaccharide administration was effective in the prevention and improvement of the lipid level, blood glucose and enzyme activities in serum of hypercholesterolemic rats.

• Journal of Life Science
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• v.24 no.12
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• pp.1301-1307
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• 2014

Adipose-derived stem cells (ADSCs) are considered promising tools for tissue regeneration. However, ADSCs have very poor proliferation capacity. Therefore, fetal bovine serum (FBS) is generally added to the culture media of ADSCs. As FBS contains many uncharacterized components that may affect cellular functions, methods for serum-free cultures of ADSCs have been widely investigated. In this study, to develop an efficient method for a serum-free culture of ADSC-T, we used an ADSC line established by introducing the simian virus 40 (SV40) T gene into primary ADSCs. We then investigated the effect of amino acids, vitamins, and other components on the growth of ADSC-T. When the ADSC-T cells were plated with DMEM/F12 serum-free medium, the cells did not proliferate, and the mixture of amino acids, vitamins, and B27 supplement did not increase the growth of the cells. However, when the ADSC-T cells were provided with serum-free DMEM/F12 after they had been cultured with serum-supplemented DMEM for 24 h, the cells proliferated, and the vitamins and B27 supplement increased the cell growth. Stem-Pro serum-free medium also appeared to be useful as a suspension culture for the ADSC-T cells. The ADSC-T cells secreted large amounts of proteins of around 70 kDa. Insulin-like growth factor (IGF) and fibroblast growth factor basic (FGF basic) were secreted by ADSC-T in larger amounts in the serum-free culture than in the serum-supplemented culture.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.30 no.3
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• pp.466-472
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• 1997

Peptides separated from fish paste washing liquid of an Alaska pollak (Theragria chalcogramma) were purified and characterized. The fish paste washing liquid (supernatant) was separated by centrifugation of fish paste homogenate. The fish paste washing liquid of $0.5\%$ concentration was hydrolyzed for 24 hour at $50^{\circ}C$ by immobilized protease in bioreactor and decomposing liquid of protein having $50\%$ decomposing rate (OPA method) was obtained. The crude peptide fractions were obtained from this liquid by Dowex 50w $(H^+)$ column chromatograpy. Purified peptides (SP-fraction peptides) were fractionated by using SP-Sepadex C-25 $(H^+)$ column chromatography. Molecular weights and amino acid compositions of these peptides were estimated by Sephadex G-50 column chromatography and HPLC, respectively. when the washed peptides was eluated with $0.6\~0.9\%\;and\;1.2\~2.0\%$ of NaCl, peptides composed of weakly basic amino acids and strongly basic amino acid were respectively eluted. Molecular weights of each peptide fractions showed the broad distribution from 1,000 Da to 3,000 Da in the order of SP-4>SP-3>SP-2>SP-1. Peptides contained a large quantity of glycine, arginine, glutamic acid, and alanine in the washed peptide and its SP-tractions, respectively.

• Journal of the Korean Institute of Intelligent Systems
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• v.20 no.6
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• pp.786-792
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• 2010

This paper presents a real-time lane detection method which can accurately find the lane-mark boundaries in complex road environment. Unlike many existing methods that pay much attention on the post-processing stage to fit lane-mark position among a great deal of outliers, the proposed method aims at removing those outliers as much as possible at feature extraction stage, so that the searching space at post-processing stage can be greatly reduced. To achieve this goal, a grid-based morphology operation is firstly used to generate the regions of interest (ROI) dynamically, in which a directional edge-linking algorithm with directional edge-gap closing is proposed to link edge-pixels into edge-links which lie in the valid directions, these directional edge-links are then grouped into pairs by checking the valid lane-mark width at certain height of the image. Finally, lane-mark colors are checked inside edge-link pairs in the YUV color space, and lane-mark types are estimated employing a Bayesian probability model. Experimental results show that the proposed method is effective in identifying lane-mark edges among heavy clutter edges in complex road environment, and the whole algorithm can achieve an accuracy rate around 92% at an average speed of 10ms/frame at the image size of $320{\times}240$.