• Korean Journal Plant Pathology
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• v.12 no.1
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• pp.129-131
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• 1996

Pecatate lyase(Pel)는 펙틴과 펙틴산을 분해하며, 기주식물의 감염에 관여한다. Erwinia rhapontici에 있어서 기주와 병원균이 병원성과의 상호관계를 구명하기 위하여 pectate lyase(Pel) 활성에 미치는 식물체 추출물과 세포벽의 효과를 검토하였다. 본 균은 glycerol이 포함된 minimal salts(MSG) 배지와 식물체 추출물이 첨가된 MSP 배지에서는 Pel 활성이 검출되지 않았다. 그러나 배추, 상추 잎, 감자 괴경, 셀러리 잎자루, 양파 인경, 당근 뿌리의 세포벽이 첨가된 MSP 배지에서는 Pel의 활성이 검출되었다. Pel을 유도하는 식물 인자는 불용성이고, 열처리에 불안전하였다.

• Research in Plant Disease
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• v.17 no.3
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• pp.381-385
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• 2011

Effects of temperatures, relative humidity, pH, and dry periods on conidial germination of Alternaria dauci were evaluated under in vitro conditions. Conidial germination was accelerated at over 95% relative humidity in $15^{\circ}C$ to $25^{\circ}C$ condition. Conidial germination was rapidly reduced at 5 regardless of relative humidity conditions. More than 50% of the conidial germination were initiated within 2 h at $25^{\circ}C$ through pH 5 to 7. The highest conidial germination of A. dauci was on 0.2% of carrot leaf extract. Conidia could survive longer than 12 h, even though its germination decreased. After a 12 h dry period, around 10% of conidia revived and germinated when conidia were hydrated again. These results could be used as the useful information on conidial germination of A. dauci and ecology of Alternaria leaf blight.

• Journal of the Korean Society of Food Science and Nutrition
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• v.30 no.1
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• pp.86-91
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• 2001

The anticarcinogenic effects of various food components on human cancer cells have received much attention in recent years. The precise effect and mechanisms of anticarcinogens in food materials on cancer cells have rarely been investigated. This study was carried out to determine the effects of Daucus carota L. leaf (DCL) extracts on cytotoxic and chemopreventive effect on human cancer cells. The experiment was conducted to determine cytotoxicity of Daucus carota L. leaf extracts on HepG2, Hela and MCF-7 cells by MTT assay. Among various partition layers of Daucus carota L. leaf, the ethylacetate partition layer (DCLMEA) at 500 $\mu\textrm{g}$/mL was shown to be most effective on MCF-7 cell lines. The four partition layers which are DCLM, DCLMH, DCLMB and DCLMH were less effecitve in inducing cytotoxicity than DCLMEA was. We also determined the induction of intracellular quinone reductase (QR) activity by adding DCL extracts on HepG2 cells. Among various partition layers of DCL extracts, DCLMH and DCLM were tested to be most effective with results such as 4.9 and 4.73 with a control value of 1.0.

• Journal of the Korean Society of Food Science and Nutrition
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• v.34 no.3
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• pp.427-432
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• 2005

This study was performed to investigate the effect of natural plant extracts on acetylcholinesterase (AChE) activity and the free radical scavenging activity. The methanolic extracts of plants were tested for AChE inhibitory activity using Ellman's colorimetric method in 96-welled microplates and antioxidant activity as the scavenging effect of 1, 1-diphenyl-2-picryl hydrazyl radical (DPPH). The results showed that AChE activities were inhibited (about 20-30%) in whole plant extract of Daucus carota var. sativa, Hypericum erectum and Fragaria yezoensis. AChE activities were inhibited (about 32-34%) in stems extract of Gingko biloba and leaves extract of Rhododendrondron yedoensa var. poukhanense. Fruit extract of Zanthoxylum schinifolium inhibited (about 18%) AChE activity. And the DPPH scavenging effects as antioxidant activity were similar to L-ascorbic acid in whole plant extract of Fragaria yezoensis and fruits extract of Comus officinalis.

• Journal of Marine Life Science
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• v.8 no.2
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• pp.166-177
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• 2023

The giant freshwater prawn (Macrobrachium rosenbergii) suffers economic losses in aquaculture facilities due to problems such as poor body color and carapace weakness. While this species is farmed on an animal-based diet, in the wild it consumes a detritus diet with a high proportion of plant matter. The plant matter they consume in the wild is not only a source of basic nutrients, but also carotenoids, which are responsible for body color. Korean goldenbell (Forsythia koreana) is a flowering tree widely distributed in Korea, and its leaves contain carotenoids comparable to yellow carrots. In this study, we investigated the effects of feeding Forsythia leaves to M. rosenbergii on their body color and health. The experimental diets were 100% commercial feed (control), CON, 80% commercial feed plus 20% Forsythia leaf powder, FP, and 80% commercial feed plus 20% unprocessed Forsythia leaves, FL, and each diet was fed to juvenile prawn with an average weight of 1.1 ± 0.2 g for 10 weeks. In terms of body color, the CON prawn were consistently clear ivory in color, while the FP and FL prawn changed from blue to dark brown as the days of rearing progressed. Survival and growth were not significantly different between CON and FP or FL. Histological comparison of the hepatopancreas revealed that the vacuole size of B cells, the constituent cells of the hepatopancreatic tubule, was significantly larger in FP and FL compared to CON. The vacuoles of B cells are responsible for the absorption and digestion of nutrients, suggesting that the supply of Forsythia leaves may have had a positive effect on the health of the M. rosenbergii. The above results show that the use of Forsythia leaves in the aquaculture of M. rosenbergii can be expected to improve body color and health without inhibiting growth.

• Food Science and Preservation
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• v.16 no.4
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• pp.512-517
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• 2009

Total phenolic content and the antioxidative activity (DPPH radical-scavenging capacity) of 29 fruits and vegetables were determined. The total phenolic contents of plum (1.06 mg GAE/g fresh weight) and cherry (0.95 mg GAE/g fresh weight) were high, whereas those of banana, carrot, and cucumber (each at 0.07 mg GAE/g fresh weight) were low. In the DPPH radical-scavenging assay, apple mango extract showed the highest value(92.8%) among the 29 ethanol extracts tested, followed by paprika (75.4%), beet (75.3%), and cherry (75.1%). In the $Lp-PLA_2$ inhibition assay, cherry (34.95% inhibition) and grape (31.03%) showed the highest $Lp-PLA_2$ activities among the 29 fruits and vegetables examined.

• Korean journal of applied entomology
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• v.14 no.4 s.25
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• pp.185-192
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• 1975

Various plant species, as many as 145 species in 43 families, were tested for susceptibility to the ordinary strain of the cucumber nosaic virus for two years from 1973 to 1974. Inoculations were made by mechanical method using carborundum. Plants in 71 species belonging to 27 families were infected. Of these species, systemic mosaic developed on the new leaves of plants in 57 species belonging to 24 families. Twenty-four species of plants, previously not reported as hosts of the CMV, were found to be infected in this experiment. These are Stellaria aquatica, Achyrauthes japonica, Agerratum houstonianum, Centipeda minima, Gillardia pulchella, Henisteptalyrate, Ixeris dentata, Saussurea uchiyamana, Brassica campestris, Lepidiumapetalum, Lobelia chinensis, Chenopodium bryoniaefolium, Carex neofilipes, Acalypha austalis, Amphicarpaea edgeworthii, Lotus corniculatus var japonicus, Phaseolus angularis, Sedum aizoom var heterodontum, Mosla punctulata, Perilla frutescens var japonica, Teucrium japonicum,. Linum usitatissimum, Mazus japonicus, Verbena hybrida. Twenty-three species reported to be susceptible by previous workers, but negative results were obtained in our experiment with Allium cepa, Celosia cristat, Daucus carota var. sativa, Artemisia asiatica, Callistenphus chinensis, Erigeron canadensis, Helianthusannuus, Tagetes eracta, Impatiens balsamina, Raphanus sativus, Ipomea batatas, Glycine max, Phaseolus vulgaris, Lilium longifolium, Papaver gomniferum, Sorghum vulgare, Triticum aestivum, Zea mays, Rumex coreanus, Potulaca grandiflora.

• Journal of the Korean Society of Food Science and Nutrition
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• v.21 no.2
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• pp.149-153
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• 1992

The antimutagenic effect of green-ye1low vegetables on the mutagenicities induced by N-methyl-N'-nitro-N-nitrosoguanidine(MNNG) and N-nitrosodimethylamine (NDMA) in Salmonella assay system and also their inhibitory effect on AZ-521 human gastric cancer cells were studied. Twenty-four items from twenty six kinds of vegetables(92%) revealed antimutagenic activity toward MNNG (p< 0.0l, 0.05). Perilla leaf, Korean cabbage, cauliflower, lettuce, mustard leaf, water dropwort, small water dropwort, carrot and burdock inhibited the mutagenicity more than 80%. The methanol extracts of the vegetables also showed the antimutagenic activity toward NDMA (p< 0.01, 0.05). Especially, perilla leaf, kale, soybean sprout and onion inhibited more than 80% of the NDMA induced mutagenicity in S. typhimurium TA100. Small water dropwort and perilla leaf exhibited the strong inhibitory effect (97~100%) on the growth of the AZ-521 human gastric cancer cells. Soybean sprout, water dropwort, broccoli, crown daisy, green red pepper, red pepper leaves, spinach, cabbage and sweet potato also inhibited growth of the cancer cells (p < 0.001~0.05).

• Journal of Korean Society of Forest Science
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• v.48 no.1
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• pp.29-39
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• 1980

This study was initiated to find out the possible insect vector and other means of transmission of jujube witches'-broom disease. 1. Eight species of leafhoppers were found to feed on both healthy and diseased jujube trees. Among these, rhombic marked leafhoppers (Hishimonus sellatus Uhler) were most abundant throughout the growing season of jujube trees in all localities surveyed. H. sellatus was far more abundant on diseased trees than healthy ones. 2. Jujube witches'-broom mycoplasma(JWM) was transmitted to jujube seedlings by Hishimonus sellatus. Jujube seedlings inoculated with H. sellatus which had been fed for 14-21 days on diseased jujube plants, developed smaller, chlorotic leaves 40-60 days after inoculation. Electron microscopy of midveins and petioles of the infected jujube seedlings revealed the presence of numerous mycoplasmalike organisms in phloem tissues. 3. Jujube witches'-broom mycoplasma was also transmitted to Vinca rosea plants by H. sellatus. Infected Vinca rosea plants developed vein clearing and marginal chlorosis of upper leaves 25-38 days after inoculation and followed by stunting and ultimate wilting and death of plant. Electron microscopy of petioles and midveins of infected Vinca rosea plant revealed the presence of numerous mycoplasmalike organisms in phloem tissues. 4. H. sellatus survived more than 30 days on jujube, Vinca rosea, carrot, celery, eggplant, hop, Calystegia japonica, Humulus japonicus, Astragalus sinicus, white clover, red clover, and radino clover. Many second generation nymphs of H. sellatus were also abserved on these plants but clovers. 5. Jujube seeds collected from witches'-broom diseased jujube trees yielded healthy seedlings.

• The Korean Journal of Pesticide Science
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• v.5 no.2
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• pp.18-25
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• 2001

Ethanol extracts from 46 vegetables were tested their insecticidal activities toward five agricultural insect pests and four stored-product insect pests. The efficacy varied with both agricultural insects/stored-product insects and vegetable species used. Potent insecticidal activities, at the concentration of 5,000 ppm, were produced from extracts of Nelumbo nucifera and Ulva lactuca against Myzus persicae, Zea ways and Z. mays (leaf) against Nilaparvata lugens, Citrullus vulgaris (seed) and U. lactuca against Plutella xylostella, N. nucifera, Z. mays, and Z. mays (leaf) against Spodoptera litura, and C. vulgaris (seed), Daucus carota, Helianthus annuus (leaf), H. annuus (flower), Lactuca sativa, and Zingiber officinale against Tetranychus urticae. Potent insecticidal activities at the concentration of 2,500 ppm were exhibited from the extracts of N. nucifera and U. lactuca against M. persicae, Z. mays against N. lugens, C. vulgaris (seed) and U. lactuca against xylostella, N. nucifera and Z. mays against S. litura, and C. vulgaris (seed), H. annuus (flower), and L. santiva against T. urticae. Against four stored-product insect pests at 50 ppm, extracts of C. vulgaris (seed) and Cucurbita moschatla (seed) against Sitophilus oryzae and C. vulgaris (seed), H. annuus (seed), and Z. officinale against Plodia interpunctellfa revealed potent insecticidal activities over 80% mortality. In tests with Callosobruchus chininsis and Lasioderma serricorne, extracts of all vegetables tested exhibited meager and no activity.