• Journal of Plant Biology
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• v.34 no.1
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• pp.37-43
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• 1991

Mesophyll protoplasts of Nicoliana labacum ($NR^{-}/SR^{+}$) and N glulinosa were electrofused with AC field of 0.5 MHz and 1 kV DC pulse for 2 ms. Fused protoplasts were selected and cultured to the green cell clusters in $MSNO_3$ medium containing 1.2 mg!ml streptomycin sulfate. Four plant lines regenerated from selected colonies showed both parental morphological characteristics of leaf and flower and these plant lines were confirmed as somatic hybrids based on electrophoretic patterns of leaf peroxidase. In XhoI restriction patterns of chloroplast DNA, these hybrid plant lines expressed both parent common restriction sites and parent specific sites. One of these hybrid lines exhibited interspecific pattern of both parental chloroplast genomes. indicating nine both parent common sites, one N labacum specific site and two N glutinosa specific sites. sites.

• The Journal of the Korea Contents Association
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• v.15 no.6
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• pp.148-156
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• 2015

Some of Chekhov's short stories strongly depicted 'women' physical desire and the sins of the flesh. Chekhov created his own eroticism in life as it is through the stories. While the Chekhovian eroticism naturally emphasized on 'sensual issue' between men and women, it is not limited to the erotic expressions only. In the stories, Chekhov is saying about the fundamental causes that why women must cheat on their men, so that he focused on their mental conflicts between corporalities and morality. Finally, women' infidelity and deviance make them cause misfortunes more than ever. Focusing on the best Chekhovian eroticism short stories, , , and , the writer explores Chekhovian eroticism's background and definition, and characteristics by analysis each text deeply.

• Korean Journal of Environment and Ecology
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• v.22 no.3
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• pp.309-319
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• 2008

Roads are an indicator of anthropogenic activity causing ecosystem disturbances and often lead to habitat fragmentation, habitat loss, and habitat isolation. The Hallasan National Park(153.4$km^2$) on Jeju Island being distinguished for its unique geology, topography, and biota has also been designated as a core area of UNESCO Man and the Biosphere(MAB) Reserve. Although the high conservation value of this park has contributed to a rapid growth of tourists and road construction, landscape changes due to roads have not been examined yet. We used GIS systems to examine the fragmentation pattern caused by roads, in relation to its zonation, elevation, and vegetation. When a buffer was applied to roads(112m width for paved roads and 60m width for both legal and illegal trails), the park consisted of 100 fragments. The ten fragments generated after applying buffer to only paved roads and legal trails ranged from $0.002km^2$ to $38.2km^2$ with a mean of $14.2km^2$, and about 7% of both nature conservation zone and nature environment zone of the park were edge. Fragments in both east and west ends of the park and around the summit exhibited relatively high shape indices with means of 5.19(for 100 fragments) and 7.22(for 10 fragments). All five legal trails are connected to the pit crater of the mountain and vegetation changed from broadleaf forests and conifer forests to grasslands with elevation, consequently resulting in dramatic fragment size reduction in grasslands at high elevation, in particular above 1,400m, where endemic and alpine plants are abundant. These results show that in Hallasan National Park the risks of habitat deterioration and habitat loss due to fragmentation may be more severe in the nature conservation zone dominated by Baengnokdam than in the nature environment zone. Therefore, current road networks of the park appear to fall short of the goal of the national park for ecosystem conservation and protection. Considering that the entire Hallasan National Park also serves as a MAB core area, conservation efforts should focus, first of all, on park rezoning and road management to mitigate habitat fragmentation.

• Parasites, Hosts and Diseases
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• v.35 no.2
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• pp.119-126
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• 1997

Acanthnmoebn sp. YM-4 is simitar to A. culbertsoni based upon morphological characteristics of trophozoites and cysts. However, based on other characteristics, pathogenicity to mice, in uitro cytotoxicity and isoenzyme patterns, Acanthomoebo sp. YM- 4 was quite different from A. culbertsoni. Restriction fragment length polymorphism (RFLP) analysis of mtDNA is useful in the classification of members belonging to the genus Acanthcmoebn. Therefore, in this study, RFLP analysis of Acnnthcmoeba mtDNAs was accomplished using five restriction enzymes: Hnelll, Hinull, Clcl, Pudl and ScE. Each restriction enzyme produced approximately 3-15 fragments (range: from 0:6 kip to 34.4 kbp) . The mtDNA genome size, calculated by the summation of restriction fragments, averaged 46.4 kbp in Acnnthamoeba sp. YM-4,48.3 kbp in A. culbertsoni and 48.8 kbp in A. polyphaic, respectively. Digested mtDNA fragments of Accnthcmoeba sp. YM-4 contained nine and seven same size fragments, respectively, from a total of 67 and 69 fragments observed in A. culbertsoni and A. polyphcgn. An estimate of the genetic divergence was 10.1% between Acanthamoebc sp. YM-4 and A. culbertsoni, and 9.9% between Acanthamoebn sp. YM-4 and A. polyphcga.

• The KIPS Transactions:PartA
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• v.16A no.2
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• pp.101-112
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• 2009

Existing techniques for defragmentation of the file system need intensive disk operation for some periods at specific time such as disk defragmentation program. In this paper, for solving this problem, we design and implement the automatic and continuous defragmentation free system by distributing the disk operation. We propose the Automatic Layout Scoring(ALS) mechanism for measuring defragmentation degree and suggest the Lazy Copy mechanism that copies the defragmented data at idle time for scattering the disk operation. We search the defragmented file by Automatic Layout Scoring mechanism and then find for empty spaces for that searched file. After lazy copy of searched fils to empty space for preventing that file from being lost, the algorithm solves the defragmentation problem by updating the I-node of that file. We implement these algorithms in Linux and evaluate them for small and defragmented file to get the layout scoring. We outperform the Linux EXT2 file system by $2.4%{\sim}10.4%$ in layout scoring evaluation. And the performance of read and write for various file size is better than the EXT2 by $1%{\sim}8.5%$ for write performance and by $1.2%{\sim}7.5%$ for read performance. We suggest this system for solving the problem of defragmentation automatically without disturbing the I/O task and manual management.

• Digital Contents
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• no.12 s.127
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• pp.114-116
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• 2003

올해 영화제에는 국내작품 55편을 포함해 전 세계 300여편의 장ㆍ단편디지털 영화가 장편부문, 단편부문, 뮤직비디오부문 등 18개 섹션에 걸쳐 소개됐다. 올해 개막작은 문명세계의 탐욕과 반문명의 모순을 그린‘휴먼네이처’로 우리 관객에게도 친숙한 미셸 공드리감독의 특별전이 준비돼 공드리 감독의 뮤직비디오, CF 등 25편이 상영됐다. 폐막작으로는 스케이트보드를 다룬 영화‘스톡트’(Stoked)가 상영됐다.

• Microbiology and Biotechnology Letters
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• v.17 no.4
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• pp.334-342
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• 1989

The Bacillus stearothermophilus cdd gene encoding cytidine deaminase (cytidine/2'-deoxycytidine aminohydrolase; EC 3.5.4.5) was isolated through shot gun cloning by oomplementation of an E. coli cdd mutation. Primarily 3.0 kbp of the exogenote was cloned into the Pstl site of pBR322 (pJSC101). By subsequent deletion and subcloning from the insert of pJSC101 with cdd$^+$ and tetracycline resistancy, about 1.35 kbp of the EcoRI$_1$/PstI$_2$ fragment containing the cdd gene was isolated as pJSC201. The minicell experiment revealed a molecular mass of 33,000 dalton for polypeptide from the cloned DNA fragment complementing the cdd gene. From the lacZ fusion of 550 bp fragment of the EcoRI$_1$/AuaI as a putative promoter region, the transcription direction of the cdd gene on pJSC201 is from EcoRI towards the PstI sites, When the cdd gene was expressed in B. subtilis ED4O (cdd$^-$, pyr$^-$) by transformation with the E. coli-B. subtilis shuttle vector, the gene expression occured more efficiently than in E. coli and the gene appears to be stably maintained in B. subtitis as well as in E. coli.

• Korean journal of applied entomology
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• v.35 no.3
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• pp.209-215
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• 1996

DNA polymorphisms were analyzed for 8 clones of the green peach aphid, Myzus persicae Sulzer, by random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR). The insect has different host preferences and was even classified into two different species, M. persicae Sulzer and Myzus nicotinae Blackman by their morphological characters, but this point is still in arguement. To identify the differences between two types of the green peach aphid by RAPD-PCR, the template DNA was extracted from 4 clones each of tobacco-feeding and non-tobacco-feeding forms and one hundred primers of 10-nucleotideslong were tested in PCR. The amplified DNAs were analyzed by agarose gel electrophoresis. Eighty-three primers gave amplified DNA fragments with 1 to 22 in number and 500 to 20,000 base pairs in length, but no amplification was observed in the other 17 primers. The average number of fragment per each amplification was about 13. In the case of 82 out of 83 random primers, band patterns of amplified DNA were identical among 8 clones, even though some differences were noticed in the intensity of specific bands. Polymorphism was detected by only one primer within the tobacco-feeding forms, but not between the two host types. The results did not detect any relationship between RAPD polymorphism and their host preference.

• Cartoon and Animation Studies
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• s.45
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• pp.435-450
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• 2016

Animation is an artificial image created by artist's imagination. Animation is defined as "the art of the animated image" in the sense that immobile images can be seed as a living creature. In other words, the term "diegesis" in modern arts genre is generally considered as something that indicates fictional world in which narration develops. Therefore, when we say that animation world is formed based on diegesis, it represents the fictionality of animation world. The problem occurs at this point. Even thought the animation world is recognized as a fictional world, we contradictorily believe that the event occurring in the screen world are real and accept them as a true message. This condition is called "quasi-emotion". Quasi-emotion is not fake bit not real either, and it is the third emotional state. It happens when we "make believe" that we believe a fictitious figure or a situation. However, in order to actively operate this "make-believe" state, an appropriate environment and props are required, specially metaphorical imagination and storytelling in short animated film. So that this article will draw a conclusion from the method that make-believe the fictional world and communicate the message through analysing the short animate film, form Disney.

• KIPS Transactions on Software and Data Engineering
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• v.9 no.3
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• pp.77-82
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• 2020

Most of the navigation in the vehicle has been developed based on a complex structure of PSF(Physical Storage Format) files, making it difficult to support incremental map updates. DB-based navigation is drawing attention as a next-generation navigation method to solve this problem. In DB-based navigation that supports incremental map updates, data fragmentation due to continuous map data updates can increase data access costs, which can lead to a decrease in search performance. In this paper, as one of the performance enhancement methods of DB-based navigation that supports incremental map updates, data fragmentation prevention techniques were presented and the performance improvement effect was verified through actual implementation.