• Journal of the Korean Society of Food Science and Nutrition
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• v.36 no.6
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• pp.766-772
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• 2007

The enzymatic hydrolysate of skipjack tuna cooking drip with good functionality was prepared by incubation with Alcalase for 30 min. For the preparation of functional seasoning sauce with enzymatic hydrolysate (SSE), the additives, such as concentrated enzymatic hydrolysate (100 mL), yeast extract powder (0.7 g), lactose (0.4 mL), liquid smoke (0.3 g) and sea tangle powder (1.4 g), were added to the enzymatic hydrolysate and boiled before filtration. The proximate composition of SSE was 11.8% for crude protein, 5.77 for pH and 11.9% for salinity. The SSE was higher in the crude protein, while lower in the salinity than commercial seasoning sauce. ACE inhibitory activity ($IC_{50}$) and antioxidative activity (PF) of SSE were 6.2 mg/mL and 1.14, respectively, which were superior to those (9.9 mg/mL in IC50 and 0.91 in PF) of commercial seasoning sauce. The free amino acid content (1,905.2 mg/100 mL) and taste value (58.65) of SSE were higher than in those (712.7 mg/100 mL and 34.30, respectively) of commercial sauce. Total amino acid content of SSE (10,965 mg/100 mL) was higher than that (4,818 mg/100 mL) of commercial sauce. The major amino acids of SSE were glutamic acid (12.2%), proline (11.0%), histidine (10.7%) and glycine (9.9%). The results suggested that SSE could be commercially sold.

• Proceedings of the Korea Society of Poultry Science Conference
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• 2001.11a
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• pp.83-85
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• 2001

This experiment was conducted to investigate the effects of pelleted food waste on performance and meat quality of broiler ducks. One hundred-forty four broiler ducks were divided into 4 groups with 3 replicates per treatment for 5 weeks. Dietary levels of food waste 0%, 25%, 50% and 75% were included in experimental diets Daily weight gain was significantly increased by addition of food waste 25% and .50%, whereas decreased(P<0.05) by addition of 75% food waste. Feed intake were not affected by the pelleted food waste supplementations. Feed efficiency was improved(P<0.05) by the addition 25% food waste, however, there was depressed(P<0.05) by the addition of 75% food waste. Carcass weight was significantly decreased(P<0.05) by the addition 75% food waste. Moisture composition of breast meat were not significantly different by 25% and 50% food waste, however, there was significantly increased by 75% food waste. As the food waste level was increased, lipid and protein composition in beast meat was significantly decreased As the food waste level was increased. pH of beast meat was increased, whereas lightness and redness of meat color were decreased. Cholesterol content was significantly decreased(P<0.05) by addition of food waste. Odor and appearance of sensory evaluation was depressed by addition of food waste, whereas taste of sensory evaluation was improved by addition of food waste. There was no pathological findings from liver, spleen and kidney in slaughtered ducks. The results of this study suggests that pelleted food waste 25% and 50% improved broiler ducks performance and meat quality in broiler ducks.

• Journal of Plant Biotechnology
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• v.38 no.1
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• pp.42-48
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• 2011

Chloroplast genetic engineering of higher plants offers several unique advantages compared with nuclear genome transformation, such as high levels of transgene expression, a lack of position effect due to site-specific transgene integration by homologous recombination, multigene engineering in a single transformation event and reducing risks of gene flow via pollen due to maternal inheritance. We established a reproducible chloroplast transformation system of potato using a tobacco specific plastid transformation vector, pCtVG (trnI-Prrn-aadA-mgfp-TpsbA-trnA). Stable transgene integration into chloroplast genomes and the homoplasmic state of the transgenome were confirmed by PCR and Southern blot analyses. Northern, immunoblot analysis, and GFP fluorescence imaging revealed high expression and accumulation of GFP in the plastids of potato leaves. This system would provide new opportunities for genetic improvement and mass production of value added foreign proteins in this crop.

• Journal of the Korean Wood Science and Technology
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• v.35 no.6
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• pp.166-174
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• 2007

To know the effect of enzymatic pre-treatment on softwood Kraft pulp, two xylanse-encoding genes, named xynl and xynll were isolated from Thrichoderma ressei. Structural genes of xylanase (XYNI, XYNII) and cellulase (EGIV-CBDII) were isolated from T. ressei and Rumicoccus albus respectively, and expressed in E. coli. bacterial culture. The specific activity of purified recombinant XYNI is higher than XYNII. The brightness of XYNI treated softwood Kraft pulp increased to 29.9%. On further sequential treatment with EGIV-CBDII and XYNI the brightness of softwood Kraft pulp were improved to 9.1 and 73% respectively. As expected the Kappa number of softwood Kraft pulp also decreased 8.1, 4.6 and 3.2% respectively. Results further indicate that this sequential combination of enzyme treatment has synergic effect for improving bleaching of softwood Kraft pulp.

• Applied Chemistry for Engineering
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• v.17 no.5
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• pp.521-526
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• 2006

A membrane bioreactor (MBR) is an effective tool for wastewater treatment with recycling. MBR process has several advantages over conventional activated sludge process (ASP); reliability, compactness, and quality of treated water. The resulting high-quality and disinfected effluents suggest that MBR process can be suitable for the reused and recycling of wastewater. An anoxic/oxic (A/O) type MBR was applied to simultaneous removal of organics and nutrients in sewage. At first, the efficiency of submerged MBR process was investigated using a hollow fiber microfiltration membrane with a constant flux of $10.2L/m^2{\cdot}h$ at each solids retention time (SRT). Results showed that protein/carbohydrate (P/C) ratio increased and total extracellular polymeric substances (EPS) remained constant with SRT increased. Secondly, A/O type MBR with a reverse osmosis (RO) membrane was employed to treat the municipal wastewater. The performance of A/O type MBR-RO process is better for the treatment of organics and nutrients than ASP-MF-RO process in terms of consistent effluents quality.

• Proceedings of the Korean Society of Postharvest Science and Technology of Agricultural Products Conference
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• 2003.10a
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• pp.148.1-148
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• 2003

버섯은 균사체와 자실체를 가진 고등균류로서 옛날부터 식용은 물론 약용으로 많이 사용되어 왔으며 부작용이 없는 저칼로리 식품으로서, 영양적인 측면과 의약품으로서의 효능을 가진 식품으로 인식되어 소비량이 날로 증가하는 추세이다. 버섯은 항균자용, 항암작용, 면역증강작용, 혈압강하 효과 및 혈중 콜레스테롤합성 억제효과 등의 다양한 생리활성을 나타내고 있어 국내에서도 버섯의 균사체 배양이 활기를 띄고 있다. 본 연구에서는 5종류의 버섯(아가리쿠스, 상황, 노루궁뎅이, 운지, 동충하초)의 균사체 배양원액을 10%, 30%, 50%, 100% 함유한 음료로서 전자공여능, 아질산염소거능, MDA cell과 A549 cell에 대한 암세포 증식억제 작용을 조사하였다. 5종류의 버섯 균사체 음료의 전자공여능은 발효원액을 30% 함유한 경우에 72∼89%였으며 100% 원액의 경우는 87∼93%의 탁월한 항산화능을 나타내었다. 균사체 음료의 아질산염 소거능은 노루궁뎅이버섯이 가장 높아서 균사체 원액은 73%, 112 희석액은 51%였으며 다음은 동충하초의 균사체 원액이 52%를 나타내었고 나머지 3종류의 버섯 균사체는 28∼36%의 아질산염소거능을 나타내었다. 균사체 발효음료 원액은 MBA cell에 대하여 5종류의 버섯 모두에서 82∼85%의 높은 암세포 증식억제작용을 나타내었고 동충하초는 1/2 희석액에서도 80%이상의 증식억제활성을 나타내었다. 균사체 발효음료 원액의 A549 cell에 대한 증식억제능은 동충하초 발효원액이 68%, 상황 발효원액이 50%로서 MDA cell에 대한 증식억제효과 보다는 효능이 낮은 편이었다. 전체적으로 5종류의 버섯 균사체 발효음료는 항산화작용, 아질산염소거능, 항암활성이 우수한 기능성 음료로 활용될 수 있을 것으로 생각된다.적으로 평가하는데 적합하나 아직 국내에선 X-선을 이용한 가공용 감자의 내부 결함특성에 대한 연구는 이뤄지지 않고 있다. 이에 본 연구에서는 가공용 감자의 내부결함 특성중 하나인 내부동공의 X-선에 의한 특성을 본 연구소에 있는 X-선 발생장비로 측정해 보고 비파괴적인 방법으로 실시간 가능성을 시험하였다. 감자는 수원 농산물 도매시장에서 2003년산 가공용 감자 (품종:선농)를 구매하여 사용하였다. 감자내 내부동공은 35 ∼ 40 kV와 5.25 mA값으로 발생된 X-선에 의해 잘 검출되는 것으로 나타나, 현장에서 충분히 활용가능 할 것으로 판단되었다. 금후, 실시간으로 내부동공을 검출할 수 있는 시스템을 개발할 계획이다.정한 결과 메탄올에서는 5% 농도차이가 그 추출효율에 유의적인 영향을 주지 않는 것으로 나타났다. 에탄올에서는 40%에서 가장 높은 함량이 측정되었고 아세톤에서는 50%에서 측정되었다. 따라서 시료의 상태와 상관없이 배 과피의 페놀성물질 추출용매로는 40∼70%의 함수 아세톤이 적합한 것으로 사료된다.en delicious는 상온에서60일 동안 보관하였을 경우, 사과표피의 색도 변화를 현저히 지연시킴을 확인하였다. 또한 control과 비교하여 성공적으로 사과에 코팅하였으며, 상온에서 보관하여을 때 사과의 품질을 30일 이상 연장하는 효과를 관찰하였다. 이들 결과로부터 대두단백질 필름이 과일 등의 포장제로서 이용할 가능성을 확인하였다.로 [-wh] 겹의문사는 복수 의미를 지닐 수 없 다. 그러면 단수 의미는 어떻게 생성되는가\ulcorner 본 논문에서는 표면적 형태에도 불구하고 [-wh]의미의 겹의문사는 병렬적 관계의 합성어가 아니라 내부구조를 지니지 않은 단순한 단어(minimal $X^{0}$ elements

• Proceedings of the KSCN Conference
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• 2003.11a
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• pp.1083-1084
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• 2003

사회생활 양식이 점차 복잡해지고 생활전반의 조직이 고도화됨에 따라 대규모 급식의 필요성이 점점 증가되고 있다. 그 동안 단체급식은 자체적으로 운영하는 경우가 많았으나, 최근 들어 위탁운영의 효율성에 대한 인식이 높아짐에 따라 위탁 운영률이 크게 높아지고 있다. 현재까지 대학급식에 관한 연구는 대학식당의 이용실태, 대학 식단의 만족도 평가, 대학급식 메뉴 관리 및 운영 등을 조사한 연구는 많았으나 운영형태에 따른 식단 분석 연구는 미흡한 실정이다. 이에 본 연구에는 서울 시내 일부 대학교의 구내에 있는 5개 식당 23가지 식단에 대하여 직영급식과 위탁급식으로 나누어 학생들에게 제공되는 메뉴를 영양가면과 다양성 면에서 평가하고, 제공된 메뉴를 학생들이 실제 섭취하는 실태를 조사하여 섭취한 식단의 영양가와 음식의 종류를 분석하였다. 본 조사에서 이용한 식단은 총 23가지였고, 그 중에서 직영급식 13가지, 위탁급식 10가지였다. 제공된 상용식단형태는 직영급식은$\ulcorner$밥+국+반찬2+김치1$\lrcorner$, 위탁급식은 $\ulcorner$한그릇+국+반찬1+김치1$\lrcorner$이었으며, 우리나라 전통의 기본패턴을 갖추고 있었다. 제공된 식단 중 한끼 영양소 함량을 평가해본 결과 여자의 경우 1/3 영양권장량을 제공하였으나, 남자의 경우는 부족하였다. 제공되는 식단의 음식군별 제공열량을 살펴보면 밥은 평균 399㎉, 한그릇음식은 425㎉로 한 그릇음식의 경우가 높은 열량을 제공하고 있었다. 반찬의 경우 1가지로부터 얻는 열량은 평균 107㎉였으며 김치의 경우는 9.84 ㎉였다. 직영급식과 위탁급식으로 나누어 살펴보면 한 그릇음식, 반찬, 김치의 제공열량이 위탁급식에서 약간 높은 경향을 보였다. 대상자의 음식의 평균 가짓수는 직영 5.5가지, 위탁 4.3가지였으며, 음식 및 반찬 가짓수가 증가할수록 섭취열량이 증가하였다. 대상자들의 섭취한 식단의 한끼당 섭취한 영양소 함량은 직영급식 524㎉, 위탁급식 494㎉였고, 단백질, 지질, 인, 철, 비타민A, 나이아신 등이 유의적으로 직영급식에서 높았다(p<0.001) 섭취한 식단의 영양소 함량은 인과 비타민C를 제외한 모든 영양소에서 권장량에 미달되었다. 섭취한 식단의 영양소적정섭취비는 대부분 0.4-0.9의 값을 보였고, 평균적정섭취비도 직영, 위탁 모두 0.68로 낮은 값을 보였다. 영양소 밀도의 경우 절대적 섭취에서 직영급식이 대부분의 영양소를 많이 섭취하고 있는 것과는 달리 위탁급식에서 철분의 밀도가 유의적으로 높았다(p<0.01). 총 대상자들이 식단에서 섭취한 열량을 살펴보면, 주식류의 경우 밥은 298㎉, 한 그릇음식은 386㎉로 한 그릇음식이 많은 열량을 제공하고 있었다. 반찬의 경우는 1가지로부터 얻는 열량은 78㎉, 김치는 8㎉였다. 이상의 결과로 볼 때, 제공량은 직영급식에서 많았으나 영양밀도 면에서는 큰 차이가 없는 것으로 보아 위탁급식의 제공량을 늘린다면 급식운영형태별 두 식단의 질적 차이가 크게 줄어들 것으로 보인다. 그러나 두 급식 형태 모두 제공식단의 영양소 함량은 다른 지역이나 연령에 비해 부족한 것으로 나타나 아침 결식 및 불규칙한 식사 등 영양상태가 좋지 못한 대학생들에게 교내 식당에서 질 높은 식사를 제공해야 할 것이다. 또한 식단 작성 시 학생들의 메뉴 기호도를 충분히 반영시키고 다양한 메뉴를 제공하는데 한계가 있다면 주.부식 외에 다양한 후식제공을 통하여 학교 급식에 대한 질을 향상시키는 노력을 해야 할 것이다.

• Korean journal of applied entomology
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• v.37 no.2
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• pp.187-191
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• 1998

For efficient and economical production of Btrc,illus tlz~rr.ingi~r1~sstirsa in NT0423 as amicrobial-control agents, a new culture medium and culture condition were developed. Five mediadesignatzd as SWI I , SW14, SW23. SW32 and SW4I were prepared ~ : i t hv arious mixture ratio ofsoybean cake and wheat bran. It was founcl that in terms of the cell growth rate and development ofsporulation of B, thrri.il~girrl.sis strain NT0423 in all SW culture media were more efficient than those inGYS and in LB media. Total cell number in all SW media showed similar values, hut SW32 lnediilm wasthe most efficient in the development of spore, which amo~~ntetod 3.7 x 10XC FUImI. Also. at the pHranging frorn 6.2 to 7.3 in the mediiun~ no ad\:erse effect was not made in the culture of B. thur-ingicnsisstrain NT0423. The optimal volume (%) of SW32 mecliuni in a 5 1 fernientor was determined as 4 8\rolume of total niediuni. resulting ill growth (4.2 x 1OTCFUlrnl) of H. t1~~irir1,yirrz.ssit.vr ain NT0423. As H.t l i ~ t r i t ~ g iw~ a~s~ csuil~tu rcd in the shakc-flash and 5 1 fcrnientor. bacterial cells were yielded to 1 X 10"CFUIml and 5 x I O1oCFLJlml.FUIml and 5 x I O1oCFLJlml.

• Korean Journal of Food Science and Technology
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• v.37 no.4
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• pp.648-655
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• 2005

Effects of two types of formula designed for commercial product on forced-swimming capacity and related biochemical parameters of Sprague-Dawley rats were evaluated. Treatment groups were fed diet supplemented with 4.56% formula A (EFA: exercise-trained and formula A-supplemented group) or B (EFB: exercise-trained and formula B-supplemented group) for 4 weeks. All groups were regularly trained twice a week to be accustomed to swimming. After 4 weeks, each group was divided into two subgroups: 90 min swimming (90-min subgroups) and swimming until exhaustion (all-out subgroups). Serum was collected, and skeletal muscles were dissected out after swimming. Forced-swimming time until exhaustion was significantly longer (p<0.05) in EFA and EFB groups than that of exercised control group. Among 90-min subgroups, serum ammonia level of EFB group was significantly lower than that of exercised control group (p<0.05). Among all-out subgroups, serum lactate levels were not significantly different among exercised control and treatment groups, whereas serum-free fatty acid levels of EFA and EFB groups were significantly lower than that of exercised control group (p<0.05). In conclusion, EFA and EFB groups enhanced forced swimming capacity of rats by increasing fat utilization and delaying accumulation of serum fatigue factor.

• Korean Journal of Clinical Laboratory Science
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• v.50 no.4
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• pp.391-398
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• 2018

Mesenchymal stem cells (MSCs) are an attractive resource for refractory patients because of their anti-inflammatory/immunomodulatory capability and multi-lineage differentiation potential. The transplantation of MSCs has led to positive results in preclinical and clinical application to various diseases, including autoimmune disease, cardiovascular disease, cancer, liver cirrhosis, and ischemic stroke. On the other hand, studies have shown that paracrine factors, not direct cell replacement for damaged cells or tissue, are the main contributors in MSC-based therapy. More recently, evidence has indicated that MSC-derived exosomes play crucial roles in regulating the paracrine factors that can mediate tissue regeneration via transferring nucleic acids, proteins, and lipids to the local microenvironment and cell-to-cell communication. The use of these exosomes is likely to be beneficial for the therapeutic application of MSCs because their use can avoid harmful effects, such as tumor formation involved in cell transplantation. Therefore, therapeutic applications using MSC-derived exosomes might be safe and efficient strategies for regenerative medicine and tissue engineering. This review summarizes the recent advances and provides a comprehensive understanding of the role of MSC-derived exosomes as a therapeutic agent.