• Title/Summary/Keyword: 단백질소화

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Characterization of Bacillus thuringiensis subsp. tohokuensis CAB167 Isolate against Mosquito Larva (모기유충에 활성 있는 Bacillus thuringiensis subsp. tohokuensis CAB167 균주의 특성)

  • Kil, Mi-Ra;Kim, Da-A;Paek, Seung-Kyoung;Kim, Jin-Su;Choi, Su-Yeon;Jin, Da-Yong;Youn, Young-Nam;Hwang, In-Chon;Ohba, Michio;Yu, Yong-Man
    • Korean journal of applied entomology
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    • v.47 no.4
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    • pp.457-465
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    • 2008
  • Eight Bacillus thuringiensis strains activated against mosquito larva were compared their characterization. Spherical-shaped parasporal inclusion of B. thuringiensis subsp. tohokuensis CAB167 was observed by phase-contrast microscopy and scanning electron microscopy. $LC_{50}$ values of B. thuringiensis subsp. tohokuensis CAB167 against Culex pipiens molestus, Culex pipiens pallens, and Aedes aegyti were 173, 190 and 580 ng/ml, respectively. B. thuringiensis subsp. tohokuensis CAB167 had a parasporal inclusion containing 4 major protein components, for example, 135, 80, 49 and 28-kDa by SDS-PAGE. Otherwise, after trypsin digestion of parasporal inclusion, SDS-PAGE was showed new protease-resistant peptides at 72 and 63-kDa. Activated toxins of isolated CAB167 were different from other reference strains on a serological by immuno-diffusion test.

A Study on the Reducing Pollutants in Non-Ruminant Manure by Increasing Feed Utilization (사료이용율 증가에 따른 비반추가축의 분뇨에 의한 공해발생 감소에 관한 연구)

  • Nahm, K.H.
    • Korean Journal of Poultry Science
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    • v.28 no.3
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    • pp.245-257
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    • 2001
  • Localization of livestock facilities leads to concentration of livestock wastes and subsequent leakage of pollutants into the environment, resulting in public concern about their effects. Nitrogen (N) and phosphorus (P) are the most harmful components of animal manure, but odor from the manure itself and the livestock facilities is also a problem. Improving the nutrient efficiency of the livestock helps to decrease excretion of these environmental contaminants. Pigs and chickens are the main experimental models used in studies to improve nutrient efficiency. Addition of feed supplements and modifying feeding systems to improve nutrient efficiency can result in significant decrease in the N, P, odor and dry matter (DM) weight of manure. Examples of these methods include the following. 1) Addition of synthetic amino acids and reducing protein contents resulted N reductions of 10∼27% in broilers, 18∼35% in chicks and layers, 19∼62% in pigs, and a 9∼43% reduction in odor in pigs. 2) Enzyme supplementation resulted in a 12∼15% reduction in DM weight in broiler manure. 3) Phvtase supplementation resulted in P reductions of 25∼35% in chickens and 20∼60% in pigs. 4) Use of growth promoting substances resulted in a 5∼30% reduction in N and a 53∼56% reduction in odor of pigs. 5) Formulating diets closer to requirements (diet modification) reduced N and P by 10∼15% each in chickens and pigs, and odor by 28∼ 79% in pigs. 6) Phase feeding reduced N and P excretion by chicken and pigs from 10∼33% and 10∼13% each, as well as odor in growing and finishing pigs by 49∼79%. 7) Use of highly digestible raw materials in feed reduced N and P excretion by 5% in chickens and pigs.

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Effects of Fertilizer Levels on Productivity and Quality of Pearl Millet (시비량이 진주조의 생산성 및 품질에 미치는 영향)

  • Keun-Yong Park;Rae-Kyung Park;Byeong-Han Choi
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.34 no.4
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    • pp.396-399
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    • 1989
  • Pearl millet (Pennisetum americanum (L.) Leeke) is a promising forage crop that is resistant to biotic and abiotic stresses, and has a capability to grow well not only in the fertile soil, but also in the poor and dry soil, The objective of the study was to investigate the effects of increased fertilizer application level on the productivity and Quality of pearl millet in the old and newly reclaimed upland soils of Suwon, Chungju, Jeonju, Jinju and Jeju in Korea from 1986 to 1988, Plant height, green fodder yield, protein content and digestibility were increased by the increased fertilizer application level. However, the increased fertilizer application caused lodging, so that the plants grown above one meter in canopy height would be desiable to be clipped remaining 20cm above the ground surface before lodging, and fed to cattle, Mean green yields of the N 45-60kg/l0a application were 12.7-13.4t/l0a in the old upland soil. Increased phosphorus and potash fertilizer application also increased productivity, especially with potash fertilizer effect being higher than that of phosphorus fertilizer, In the newly reclaimed upland soil, improved and doubled fertilizer application plot of 60-60-40-4000kg/10a in N-P$_2$O$\sub$5/-K$_2$O-Compost was 38% higher being 12.6t/10a of green fodder yield as compared with standard fertilizer application plot, Pearl millet productivity and Quality were higher than those of maize and sorghum/sudan grass hybrids, particularly in green fodder yield, protein content and digestibility.

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Polysaccharide isolated from fermented barley extract activates macrophages via the MAPK and NF-κB pathways (보리발효추출물로부터 분리한 다당의 대식세포 활성화 및 신호 전달)

  • Kim, Han Wool;Jee, Hee Sook;Shin, Kwang-Soon
    • Korean Journal of Food Science and Technology
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    • v.50 no.5
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    • pp.555-563
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    • 2018
  • Barley has nutritional benefits due to its high dietary fiber content; therefore, the intake of whole barley grains is recommended. However, barley is often consumed in the fermented form because of the improved texture and digestibility. The present study was designed to elucidate the intracellular signaling pathway for macrophage activation by the polysaccharide BF-CP from fermented barley. BF-CP is a neutral polysaccharide, composed of neutral sugars, including glucose (70.7%), xylose (11.4%), and arabinose (9.0%). BF-CP exhibited macrophage-stimulatory activity by inducing the production of interleukin (IL)-6, tumor necrosis factor $(TNF)-{\alpha}$, and nitric oxide in RAW 264.7 macrophages. Further, BF-CP treatment strongly increased the IL-6 and $TNF-{\alpha}$ gene expression in a concentration-dependent manner. Signal transduction experiments using immunoblotting showed that BF-CP phosphorylated mitogen-activated protein kinases (MAPKs), such as c-Jun N-terminal kinase, extracellular signal-regulated kinase, and p38, and nuclear factor $(NF)-{\kappa}B$, in RAW 264.7 cells in a concentration-dependent manner. These results suggest that BF-CP activates the macrophages via MAPK and $NF-{\kappa}B$ pathways, and also induces an increase in the production of cytokines.

Physico-Chemical Characteristics of Sewage Sludge under Electron Beam Irradiation (전자선으로 처리한 하수슬러지의 특성연구)

  • Shin, Kyung-sook;Kang, Ho;Bang, Ky-youn
    • Journal of Korean Society of Environmental Engineers
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    • v.22 no.7
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    • pp.1225-1232
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    • 2000
  • This study was carried out to define the effect of electron beam irradiation on the physico-chemical characteristics of sewage sludges. The experimental evidence showed that both pH and alkalinity of irradiated sludge were generally increased as the dose of irradiation increased. It was found that the soluble protein concentration (SPC) and soluble chemical oxygen demand (SCOD) from the sludge right after electron beam irradiation at 3kGy(kilo-joule/kg) increased 2.2 times and 10 times respectively more than those sludges without electron beam treatment. This highly solubilized organics could be resulted in a good soluble substrate for the subsequent anaerobic digestion process. The specific resistance of filtration (SRF) tests showed that sludge dewaterability under electron beam irradiation at 6kGy was found to be 8.8 times higher than that of unirradiated sludge. The sludge dewaterability seemed to be directly related to the dosage of electron beam irradiation up to 10kGy. However, the efficiency of sludge dewaterability tended to be smaller with higher applied irradiation dose. In comparing treatments by different inorganic chemical conditioner with irradiated and unirradiated sludges, it appeared that the dewaterability with irradiated sludge was approximately 4-10 times better that that of unirradiated sludge. Even electron beam treatment itself could replace the result from the sludge conditioned with inorganic chemical coagulants.

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Effects of Seeding Rate on Forage Yield and Feed Value of Rye in Paddy Field (파종량이 답리작 호밀의 수량과 사료가치에 미치는 영향)

  • ;Je-Cheon Chae
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.36 no.6
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    • pp.513-520
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    • 1991
  • The objective of experiment was to investigate the effects of seeding rate on forage yield and feed value, and to clarify the optimum seeding rate of rye in middle west area when cultured in paddy field. The field experiment treated with 5 levels of seeding rate was carried out at Yesan area in 1989. The plant height and the number of tillers per m' were increased but the dry matter rate was decreased as seeding rate increased. The fresh and dry weights of rye plant were increased as seeding rate increased. The highest yield was obtained from the harvest of May 15 and 25 in Yesan area. The TDN content and RFV were decreased by the increased seeding rate and delayed cutting date. The highest TDN yield was obtained from the seeding rate of 18kg/l0a and the next highest yield was obtained from the 23kg and 28kg/l0a seeding rate. The content of ENE, NEL, NEM, and NEG was decreased by the increased seeding rate and delayed cutting date. The highest ENE and NEL yields were obtained from the seeding rate of 23kg/l0a and May 15 harvest. The highest NEM and NEG yields were obtained from the seeding rates of 18kg/l0a and May 15 harvest, and of 18-28kg/l0a and May 5 harvest, respectively.

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Effects of Dietary Soybean Meal in Rainbow Trout (Oncorhynchus mykiss) III. Bioavailability of Phosphors (무지개송어(Oncorhynchus mykiss) 사료원으로써 대두박의 이용 효과 III. 인(P)의 이용성)

  • 김병기
    • Journal of Aquaculture
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    • v.10 no.3
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    • pp.347-356
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    • 1997
  • Feeding experiment was conducted to evaluate effects of dietary soybean meal (SBM) on the phosphorus bioavailability of rainbow trout (Oncorhynchus mykiss). Seven diets, containing 0~70% SBM, were made based on iso-nitrogenous and iso-caloric basis of 44% crude protein. Ajpparent digestibility of phosphorus was measured 60 and 120 days after feeding. The digestibility coefficient increased gradually as the level of SBM increased up to 46~58%, but decreased at 58~70% SBM level. Phosphorus concentrations in feces decreased with an increased in dietary SBM level. Patterns of post-prandial phosphorus excretion were not similar to those of ammonia excretion, but the amount decreased with the increased SBM level in the diets. Total excretion of phosphorus for 24 hours was 19.68mg.kg-1 body wt..day-1 in the control group, but excreted phosphorus by the 58% and 70% SBM groups was 63.4% and 56.1% of the control group, respectively. Phosphorus concentration of whole body decreased with an increase in dietary SBM level for 120 days of feeding. However, there were no significant differences in phosphorus concentrations of liver among the experimental groups, except for 70% SBM diet group.

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The Effect of Three Different Grain Diets on the Growth of Albino Rats (곡식 혼식이 흰쥐의 성장에 미치는 영향)

  • Kim, Sook-He;Kim, Kyung-Ja
    • Journal of Nutrition and Health
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    • v.5 no.4
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    • pp.177-187
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    • 1972
  • The effect of three different grains supplemented diet as a dietary carbohydrate source on the growing rat, growth, food intake, FER and PER value, excretion of urinary arid fecal nitrogen and glucose and the level of glucose and cholesterol in serum blood was studied. Forty males and same number of females of Albino rats weighing male $58{\pm}2g$, female$62{\pm}2g$ were divided into eight experimental group, five male and female in each and fed rice diet, rice supplemented with wheat and rice supplemented with barley diet for 14 weeks. Under this condition wheat supplemented diet group shows the best FER, PER and body weight gain value. Especially in female group shows better FER, PER and body weight gain value compare to control diet group. Under the male groups wheat supplemented diet group shows lower FER, PER value and body weight compare to the control group but higher than those of barley diet group or rice alone. In female group the nitrogen retention shows also highest value by wheat supplemented diet group. By male group the wheat supplemented diet group shows not better value compare to the control group, but higer than barley supplemented group or rice alone. The nitrogen excretion through feces was significantly higher by grain supplemented diet group compare to grain the 20% sugar casein diet. The blood cholesterol content was slightly decreased by the control group which was fed disaccaride, compare to groups which were fed polysaccaride. But the free cholesterol to esterified cholesterol ratio shows lower value by control group than grain groups except the 70% rice supplemented with 30% barley group.

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Importance of National Crop Productivity in View of CO2 Balance (탄산가스 배출과 국내 작물 생산성의 중요성)

  • Shin, Jin-Chul;Kim, Jun-Hwan;Shon, Ji-Young;Yang, Woon-Ho;Lee, Chung-Guen;Yang, Won-Ha
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.10 no.2
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    • pp.65-68
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    • 2008
  • Crop production in a country is very crucial not only in a country's crop self-sufficiency, but also in carbon recycle. Crop can capture and store the emitted $CO_2$ by cereal food consumption. However, imported cereal food is not concerned in carbon recycle and this hinders the accurate estimation of carbon recycle. Korea imports major grain products including wheat, corn, soybean, and rice and about 90% of the grain composition consists of carbohydrate, protein and lipid. Carbon portions in carbohydrate, protein and lipid are 45, 53, and 77%, respectively. When the carbon portions are digested and converted into $CO_2$ completely, one ton of rice, wheat, corn, and soybean can release 1.51, 1.63, 1.56, and 1.56 tons of $CO_2$ to the atmopshere, respectively. Based on this premise, the evolved $CO_2$ calculated from imported grains for the last 4 years in Korea was over 21 MT of $CO_2$. This amount is equivalent to 4.8 billion US dollars in the conception of carbon tax. These results suggest that a decrease in a cereal import(i.e, an increase in a country's crop self-sufficiency) can lower $CO_2$ release to the atmosphere.

The Study on Treatment of Poultry Waste by Earthworms, and the Effect of Feeding Earthworms Meal on the Performance of Broilers and Laying Hens, and Safety of Meat and Egg (지렁이를 이용한 계분처리 그리고 지렁이를 이용한 계육 및 계란의 생산과 안전성에 관한 연구)

  • Son, Jang-Ho
    • Korean Journal of Organic Agriculture
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    • v.17 no.1
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    • pp.63-82
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    • 2009
  • This review was conducted to the study on treatment of poultry waste by earthworms, and the effect of feeding earthworms meal on the performance of broilers and laying hens, and safety of meat and egg. 1. The pro-environmental chicken house was building in Daegu National University of Education, Daegu city, chicken wastes will be turn into high quality compost by vermicomposting uses earthworms in the house. 2. The earthworm meal (EWM) has a high proteinic content and a balanced amino acid and fatty acid profile, therefore most feeding applications has been evaluated with mono-gastric animals. 3. The dietary supplementations of 0.2 to 0.4% EWM were effective in improve digestibility of crude protein of diet resulted improved broiler performance in broiler chickens. 4. The supplementing 0.2 to 0.6% of earthworm meal in the laying hens diet, improves the laying performance and egg quality, especially ratio of egg yolk n-6/n-3 fatty acids contents. 5. As, Cd, Cr, Hg and Pb were detected at level of 4.41, 1.23, 1.18, 0.00 and 3.39ppm in earthworm meal, respectively, but those were not detected in the chicken meat and egg. therefore supplementing 0.6% of EWM in the chicken diet, it still did not affect meat and egg safety. 6. These results indicated that vermicompositing uses earthworms good a subject matter in the pro-environmental animal husbandry.

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