• Korean journal of applied entomology
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• v.51 no.3
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• pp.255-263
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• 2012

High frequency sounds disrupt physiological processes, such as feeding behavior, development and immune responses of Spodoptera exigua. We analyzed high frequency sounds with respect to biochemical changes in S. exigua. High frequency sound (5,000 Hz, 95 dB) suppressed protein synthesis and secretion of midgut epithelium. It also significantly inhibited a digestive enzyme activity of phospholipase $A_2$. The gene expression of three different heat shock proteins and apolipophorin III was altered, particularly in midgut tissue in response to high frequency sound treatments. High frequency sound treatments significantly increased sugar and lipid levels in hemolymph plasma. These results suggest that high frequency sounds are a physiological stress that induces biochemical changes in S. exigua.

• Journal of The Korean Society of Grassland and Forage Science
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• v.12 no.3
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• pp.134-140
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• 1992

사일리지의 사양가치에 가장 크게 영향을 미치는 요인은 사초의 종류에 기인되는 화학조성분이며 사일리지를 적기에 제조하므로 단위면적당 최고의 양분수량을 거둘 수 있다. 유기산의 생성량이 많고 낙산이나 암모니아태질소의 함량이 많으면 사일리지의 품질이 떨어지고 섭취량이 감소되므로 사일리지 품질 증진을 위한 연구방향은 발효를 감소시키고 단백질의 분해를 막는 것이다. 사일리지의 발효에 영향을 미치는 중요한 요인으로는 사초의 화학조성분인 수분, 수용성탄수화물 등과 제조기술에 크게 좌우되는 공기(산소)혼입량이다. 재료의 수분함량은 사일리지 발효품질에 큰 영향을 미치며 수분이 많은 재료는 적당히 예건되면 젖산발효는 덜 제한되는 반면 불필요한 발효가 줄어들고 낙산발효를 억제할 수 있어 품질이 향상되고 기호성이 증진된다. 또한 사초를 알맞은 길이로 절단하므로 답압이 잘되어 공기 배출이 양호해 혐기상태의 유지가 가능하게 되고 사일리지의 취급또한 편리해 진다. 사일리지 첨가제는 재료를 적절히 예건하지 못할 때 필요하고 또한 특별한 조건하에서는 권장되고 있다. 이와같이 사일리지 발효에 도움을 준다는 가능성에도 불구하고 첨가제의 잇점은 양질 사일리지 제조를 위한 제반 처리를 대신할 수 없다. 양건 건초제조에 소요되는 기간은 기후에 크게 영향을 받게 된다. 건초제조 과정 중 포장에서 생기는 건물손실은 외기에 오랫동안 노출되면 잎이나 연한 줄기 부분이 부서지고 카로틴이 파괴된다. 또한 말리는 과정에 비를 맞힐 경우 양분이 용해되어 소화되기 쉬운 영양소와 건물 손실이 증가되며 섬유소함량이 증가되어 소화율과 섭취량이 감소된다. 그러므로 일기 예보에 따라 좋은 날씨가 $3\sim4$일 계속되는 시기를 택하여 적기 수확하고, 줄기의 압쇄(condition), 건조시 풀의 두께를 얇게, 뒤집기, 적절한 수분함량일 때 거둬들이는 조치가 필요하다.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.29 no.2
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• pp.135-142
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• 1996

Two experiments were conducted to investigate the utilization of various types of fish meal as dietary protein sources in Korean rockfish (Sebastes schlegeli). In the first experiment, averaging 5.7 g fish were divided into two groups and fed one of diets containing $55\%$ steam-dried white fish meal (SWFM) or $55\%$ brown fish meal (BFM) for 45 days. Weight gain and feed efficiency from fish fed SWFM were significantly (P<0.05) higher than those from fish fed BFM diet. However, daily feed intake, protein retention and lipid retention were not influenced by the two different diets. In the second experiment, averaging 86.6 g fish were divided into six groups and fed one of diets containing $56\%$ SWFM, $28\%\;SWFM+31\%$ flame-dried white fish meal (FWFM), $62\%\;FWFM,\;28\%\;SWFM+31\%\;BFM,\;56\%\;BFM$, or $28\%\;SWFM+29\%$ hydrolyzed fish protein (HFP) for 21 weeks. No significant differences were found among fish fed the six different diets in weight gain, feed efficiency, condition factor, whole body composition, and relative liver weight. Results of the present studies indicated that fish size should be considered in selecting feed ingredients and also feed cost could be lowered by replacing SWFM with FWFM or BFM in growing Korean rockfish diet.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.24 no.2
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• pp.144-151
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• 1991

Quality stability and utilization of sardine protein concentrates were investigated. pH, water activity and amino-nitrogen contents of autoclaved and boiled products were little changed during the storage of 60 days. Available lysine contents of the both products at the initial stage of storage were 5.58g/16g-N and 5.69g/16g-N, respectively. But the available lysine contents and digestibility of the both products decreased slightly with increasing of storage time. Lipophilic and hydrophilic brown pigment formation of the both products increased during storage of 60 days, but peroxide value(POV) and thiobarbituric acid(TBA) value decreased. Total amino acid contents of the both products were in the range of $88.99{\~}89.90g/16g-N$, and the predominant ones were glutamic acid, aspartic acid, leucine and lysine. From the sensory scores of model snack, it is concluded that the sardine protein concentrate can be used as a source material for snack.

• Journal of Animal Science and Technology
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• v.52 no.2
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• pp.117-124
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• 2010

An in situ ruminal disappearance trial and an in vivo sheep metabolism trial were conducted to evaluate the nutritional value of spent mushroom substrate (SMS, originated from Pleurotus osteratus bed-type cultivation). The raw SMS was ensiled (ESMS) for 30 days with 5% (w/w, DM basis) molasses, 0.5% (v/w) yeast (Saccharomyces cerevisiae) and 0.5% (v/w) lactic acid bacteria (Lactobacillus plantarum). Two ruminally cannulated Holsteins (average BW 620 kg) were used to evaluate in situ disappearance. Six sheeps (average BW 48 kg) were fed, in $3{\times}3$ Latin square design, rice straw alone (Control), 25% (ESMS-25) and 50% (ESMS-50) of rice straw were replaced with ensiled SMS. For an in situ trial, ruminal DM and neutral detergent fiber (NDF) disappearance of SMS were increased after ensiling (P<0.05). For a sheep trial, protein and fiber (NDF, acid detergent fiber, crude fiber) digestibilities were decreased (P<0.05), crude ash digestibility was increased (P<0.001), and nitrogen retention was not affected (P>0.05) as rice straw was replaced with ensiled SMS. Ruminating time was decreased by an average of 28% by feeding ensiled SMS (P<0.05). Ensiled SMS (Bed-type cultivation) had 76% of energy value of rice straw. Consequently, ensiled SMS (Bed-type cultivation, 100% cotton waste) could be used as a roughage source appropriate for maintenance type rations for ruminants.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.34 no.2
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• pp.84-90
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• 2001

This study was performed to know the effect of depolymerized alginate obtained by hydrolysis of alginate through a heating process at $121^{\circ}C$ on intestinal environment, Rats were fed with diets containing $1\%$, $5\%$, and $10\%$ of each depolymerized alginate (HAG-10, HAG-50, HAG-100 and alginate) for 35 days, The changes of weight, moisture content, pH and volatile basic nitrogen (VBN) of fecal, and a short chain fatty acids (SCFA) were checked in the rats. The fecal weight and moisture content were the highest in rats fed with alginate diets (p<0.01), followed by HAG-100, HAG-50 and HAG-10 in order. The $5\%$ of HAG-50 diets induced a significant increase in contents of protein and lipid of feces, resulting in the decrease of apparent digestibility of protein and lipid (p<0.01). The pH and VBN content in feces of the rats decreased in $5\%$ and $10\%$ of HAG-50 diets, but $10\%$ of HAG-100 diets; $5\%$ and $10\%$ of alginate diets brought about an increase of fecal pH and VBN (p<0.01), The amount of n-butyric acid in feces was increased while propionic and acetic acid contents decreased significantly (p<0.01) in diets containing $5\%$ and $10\%$ HAG-50. However, the feces of rat fed diet containing $5\%$ and $10\%$ alginate showed a tendency to being opposite in results than that of HAG-50.

• Applied Biological Chemistry
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• v.33 no.2
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• pp.109-115
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• 1990

This study was carried out to investigate the effect of L-cysteine and sodium sulfite on heat inactivation of soybean trypsin inhibitor(STI) and to determine cultivar difference in the inhibitory activity of STI. Effect of L-cysteine and sodium sulfite at different concentrations, pH's, and lengths of treatment on inactivation of STI were studied. The inactivation of STI was spectrophotometrically determined by measuring the rate of production of p-nitroaniline from synthetic substrate, N-benzoyl-DL-arginine-p-nitroanilide. Addition of L-cysteine and sodium sulfite increased magnitude of heat inactivation and greatly inhibited the re-activation of STI. There was no difference STI inactivation in among soybean cultivars employed. The trypsin inhibitory activity of STI of the soybean cultivars ranged from 64.7 to 86.4 TIU(trypsin inhibitor unit) per gram soyflour and the decreasing order of the TIU was Jangback>Hill>Jangyeab, Kwangkyo> Danyeab>Dangkyung>Paldal, Saeal, Duckyu>Hwangkeum. Inhibitory activity of STI was correlated with cysteine $content(r=0.6568^*)$ and with $digestivility(r=-0.7695^{**})$, but there was no correlation between the protein content and the inhibitory activity of STI.

• Food Science and Preservation
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• v.23 no.1
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• pp.97-103
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• 2016

The antioxidant and digestive enzyme inhibitory effects of Eisenia bicyclis extracted by various extraction methods (RE, reflux extraction; SE, ultrasonification extraction; AE, autoclave extraction; LE, low-temperature high-pressure extraction) were investigated. The extraction yield (55.21%) and the laminarin (39.03%), fucoidan (24.75%), total polyphenol (115.68 mg GAE/g) and flavonoid (36.67 mg RHE/g) contents of AE were higher than those in other methods. The DPPH radical (86.60%, 500 mg%), ABTS radical (58.56%, 25 mg%), nitrite (86.38%, 100 mg%) scavenging activities of the Eisenia bicyclis extracted by AE were higher than those of Eisenia bicyclis extracted by other methods. The ABTS radical and nitrite scavenging activities were above 98% in all tested Eisenia bicyclis extracts and these activities were dependent on its concentration. The inhibitory effects of AE against amylase (50 mg%) and ${\alpha}$-glucosidase (5 mg%) were 64.76% and 86.71%, respectively. The AE showed the best inhibitory effect of Eisenia bicyclis extracts (50 mg%) against trypsin (24.37%) and ${\alpha}$-chymotrypsin (49.05%), respectively. These results suggest that Eisenia bicyclis extracted by AE can be used as a bioactive and functional material in the food industry.

• Journal of the Korean Society of Food Science and Nutrition
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• v.30 no.6
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• pp.1246-1252
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• 2001

A feeding experiment was conducted on rats to investigate the inhibitive effects of baik-kimchi against the toxicity of Amaranth (Am, Food Red No.2) on growth, fecal weights and bulk, and the digestibility of protein and lipid. The body weight of the Am diet group (5% Amaranth) remained at the lowest level of all diet groups, because of diarrhea symptoms throughout the feeding period, 229.8 g of total body weight or 79.4% of the basal diet group (289.4 g). However, in the baik-kimchi diet group (10% baik-kimchi powder+5% Amaranth) no diarrhea symptoms were evident and total body weight remained at a similar level (280.3 g) to the basal diet group. It therefore appears that baik-kimchi offers an inhibitive effect against the toxicity of Am. In the baik-kimchi diet group, the total fecal weight (173.99 g/3 weeks) was 15 times that of the basal diet group (11.13 g/3 weeks) suggesting that baik-kimchi has the effect of increasing fecal bulk. The large intestine and the cecum of the baik-kimchi diet group were significantly (p<0.05) heavier than those of the Am diet group. The digestibility of protein (50.26%) and lipid (58.37%) in the Am diet group was very low in comparison with these in the baik-kimchi diet group (87.30% and 92.12%, respectively). This result indicates that the low digestibility of these nutrients in the Am diet group was directly responsible for the decrease of weight. Consequently, it is demonstrated that baik-kimchi can inhibit the effects of the toxicity of Am in rats.

• Journal of Sericultural and Entomological Science
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• v.28 no.2
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• pp.38-47
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• 1986

Silkworm(Bombix mori) were reared with modified artificial diets which were mixed with, as additives, leaf powders of Erigeron canadensis L., Cassia tora L., Cyperus anuricus Var.Laxus and Vigna Sinensis NEDL. The effects of additives on silkworm characteristisc of tested plants were summarized as follows ; 1. About 2-5% addition on dry weight base of leaf powders of E. canadensis, C. tora, C. anuricus or V.sinensis to the basic artificial diet promoted feeding response and digestion and resulted in good practical silkworm characteristics. The addition of V.sinensis and C.anuricus showed especially good effects. 2. The syneristic effect between different plant species was not recognized based on the feeding response and digestion of silkworm reared with various combinations of 2-4 different plant additives. 3. Electrophoretic zymograms of estrase, protease and phosphatase on haemolymph, intestine and silkgland were significantly different among treatments. In general, 1 or 2 more electrophoretic bands were detected when feeding response and digestion were promoted. 4. Contents of starch, crude fat, crude protein and inorganic base were apparently higher in the tested plants than in mulberry leaves. However, no volatile ingredent which is directly realted with feeding response was identified.