• Title/Summary/Keyword: 단계별추출

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Variation of antigenic proteins of eggs and developmental stages of Paragonimus westermani (폐흡충 발육 단계에 따른 항원 단백질의 변화)

  • Yoon KONG;Joon-Yong CHUNG;Doo-Hee YUN;Lee-Su KIM;Shin-Yong KANG;Akira ITO;Liang MA;Seung-Yull CHO
    • Parasites, Hosts and Diseases
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    • v.35 no.3
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    • pp.197-202
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    • 1997
  • Diagnosis of early paragonimiasis is difficult because parasitological evidence is not easily obtained. Antibody tests have been proposed as a good substitute for classical diagnostic techniques. Using the crude extracts of Parnsonimus westermnni eggs, metacercariae. 4- and 7-week Juveniles, and 16-week adults as antigens, we observed the early antibody responses. Sera were obtained from 4 experimental cats, fed 50 metacercariae each, at intervals until 13 weeks post-infection. Antibody (IgG) responses were identified by ELISA using extracts of 4-week juveniles, followed by those of 7- and 16-week worms. Antibody responses were minimal against the metacercarial extracts. Antibodies to p. westemoni egg extracts were elevated after 10 weeks post-infection. In immunoblot analysis, more than nine protein bands in 4-week juveniles reacted with the early infection sera. Antigenic proteins in adult worms were different from those of juveniles. After four weeks of infection, 32 and 35 kDa bands in the adult extracts were increasingly reactive. Egg specific proteins at 28, 46 and 94 kDa were reactive only after 10 weeks. Antigenic components reactin료 to the early infection sera changed during the maturation stages of P. westermani; almost all juvenile antigens were replaced by adult antigen components.

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Transcriptome Analysis of Longissimus Tissue in Fetal Growth Stages of Hanwoo (Korean Native Cattle) with Focus on Muscle Growth and Development (한우 태아기 6, 9개월령 등심 조직의 전사체 분석을 통한 근생성 및 지방생성 관여 유전자 발굴)

  • Jeong, Taejoon;Chung, Ki-Yong;Park, Woncheol;Son, Ju-Hwan;Park, Jong-Eun;Chai, Han-Ha;Kwon, Eung-Gi;Ahn, Jun-Sang;Park, Mi-Rim;Lee, Jiwoong;Lim, Dajeong
    • Journal of Life Science
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    • v.30 no.1
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    • pp.45-57
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    • 2020
  • The prenatal period in livestock animals is crucial for meat production because net increase in the number of muscle fibers is finished before birth. However, there is no study on the growth and development mechanism of muscles in Hanwoo during this period. Therefore, to find candidate genes involved in muscle growth and development during this period in Hanwoo, mRNA expression data of longissimus in Hanwoo at 6 and 9 months post-conceptional age (MPA) were analyzed. We independently identified differentially expressed genes (DEGs) using DESeq2 and edgeR which are R software packages, and considered the overlaps of the results as final-DEGs to use in downstream analysis. The DEGs were classified into several modules using WGCNA then the modules' functions were analyzed to identify modules which involved in myogenesis and adipogenesis. Finally, the hub genes which had the highest WGCNA module membership among the top 10% genes of the STRING network maximal clique centrality were identified. 913(6 MPA specific DEGs) and 233(9 MPA specific DEGs) DEGs were figured out, and these were classified into five and two modules, respectively. Two of the identified modules'(one was in 6, and another was in 9 MPA specific modules) functions was found to be related to myogenesis and adipogenesis. One of the hub genes belonging to the 6 MPA specific module was axin1 (AXIN1) which is known as an inhibitor of Wnt signaling pathway, another was succinate-CoA ligase ADP-forming beta subunit (SUCLA2) which is known as a crucial component of citrate cycle.

A Study on the Applicability of Soilremediation Technology for Contaminated Sediment in Agro-livestock Reservoir (농축산저수지 오염퇴적토의 토양정화기술에 대한 적용성 연구)

  • Jung, Jaeyun;Chang, Yoonyoung
    • Journal of Environmental Impact Assessment
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    • v.29 no.3
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    • pp.157-181
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    • 2020
  • Sediments from rivers, lakes and marine ports serve as end points for pollutants discharged into the water, and at the same time serve as sources of pollutants that are continuously released into the water. Until now, the contaminated sediments have been landfilled or dumped at sea. Landfilling, however, was expensive and dumping at sea was completely banned due to the London Convention. Therefore, this study applied contaminated sedimentation soil of 'Royal Palace Livestock Complex' as soil purification method. Soil remediation methods were applied to pretreatment, composting, soil washing, electrokinetics, and thermal desorption by selecting overseas application cases and domestically applicable application technologies. As a result of surveying the site for pollutant characteristics, Disolved Oxigen (DO), Suspended Solid (SS), Chemical Oxygen Demand (COD), Total Nitrogen (TN), and Total Phosphorus (TP) exceeded the discharged water quality standard, and especially SS, COD, TN, and TP exceeded the standard several tens to several hundred times. Soil showed high concentrations of copper and zinc, which promote the growth of pig feed, and cadmium exceeded 1 standard of Soil Environment Conservation Act. In the pretreatment technology, hydrocyclone was used for particle size separation, and the fine soil was separated by more than 80%. Composting was performed on organic and Total Petroleum Hydrocarbon (TPH) contaminated soils. TPH was treated within the standard of concern, and E. coli was analyzed to be high in organic matter, and the fertilizer specification was satisfied by applying the optimum composting conditions at 70℃, but the organic matter content was lower than the fertilizer specification. As a result of continuous washing test, Cd has 5 levels of residual material in fine soil. Cu and Zn were mostly composed of ion exchange properties (stage 1), carbonates (stage 2), and iron / manganese oxides (stage 3), which facilitate easy separation of contamination. As a result of applying acid dissolution and multi-stage washing step by step, hydrochloric acid, 1.0M, 1: 3, 200rpm, 60min was analyzed as the optimal washing factor. Most of the contaminated sediments were found to satisfy the Soil Environmental Conservation Act's standards. Therefore, as a result of the applicability test of this study, soil with high heavy metal contamination was used as aggregate by applying soil cleaning after pre-treatment. It was possible to verify that it was efficient to use organic and oil-contaminated soil as compost Maturity after exterminating contaminants and E. coli by applying composting.

Development of Cloud Detection Method Considering Radiometric Characteristics of Satellite Imagery (위성영상의 방사적 특성을 고려한 구름 탐지 방법 개발)

  • Won-Woo Seo;Hongki Kang;Wansang Yoon;Pyung-Chae Lim;Sooahm Rhee;Taejung Kim
    • Korean Journal of Remote Sensing
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    • v.39 no.6_1
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    • pp.1211-1224
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    • 2023
  • Clouds cause many difficult problems in observing land surface phenomena using optical satellites, such as national land observation, disaster response, and change detection. In addition, the presence of clouds affects not only the image processing stage but also the final data quality, so it is necessary to identify and remove them. Therefore, in this study, we developed a new cloud detection technique that automatically performs a series of processes to search and extract the pixels closest to the spectral pattern of clouds in satellite images, select the optimal threshold, and produce a cloud mask based on the threshold. The cloud detection technique largely consists of three steps. In the first step, the process of converting the Digital Number (DN) unit image into top-of-atmosphere reflectance units was performed. In the second step, preprocessing such as Hue-Value-Saturation (HSV) transformation, triangle thresholding, and maximum likelihood classification was applied using the top of the atmosphere reflectance image, and the threshold for generating the initial cloud mask was determined for each image. In the third post-processing step, the noise included in the initial cloud mask created was removed and the cloud boundaries and interior were improved. As experimental data for cloud detection, CAS500-1 L2G images acquired in the Korean Peninsula from April to November, which show the diversity of spatial and seasonal distribution of clouds, were used. To verify the performance of the proposed method, the results generated by a simple thresholding method were compared. As a result of the experiment, compared to the existing method, the proposed method was able to detect clouds more accurately by considering the radiometric characteristics of each image through the preprocessing process. In addition, the results showed that the influence of bright objects (panel roofs, concrete roads, sand, etc.) other than cloud objects was minimized. The proposed method showed more than 30% improved results(F1-score) compared to the existing method but showed limitations in certain images containing snow.

Development of Measurement and Evaluation Process for Risk-based Configuration Factors in Mixed Used Development in Urban Regeneration Projects (복합용도 도시재생사업에서의 리스크 기반 변화요인 측정 및 평가 프로세스 개발)

  • Son, Myung-Jin;Hyun, Chang-Taek
    • Korean Journal of Construction Engineering and Management
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    • v.13 no.6
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    • pp.94-106
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    • 2012
  • In recent years, the risks and uncertainties associated with mixed used development in urban regeneration projects which have actively been implemented at home and abroad have been on the rise due to the uncertainties of the initial business plan, difficulty of financing, increase in total cost and schedule delay. To cope with rapid social and economic changes and optimize benefits, a risk-based configuration management process that considers life cycle is required, along with accurate planning in the early stage of the business. In addition, it is necessary to prepare measures that can respond to the evaluation and measurement of the configuration factors in relation to the business process. However, the focus of previous studies on configuration management in the field of construction was mainly on humanities and the sociological aspects such as organization, leadership, ideology and similar concepts. There has been limited research on the process and measurement and evaluation methods for configuration factors required in decision-making on the risks and changes that can occur in the actual project implementation phase. Accordingly, in this study, we defined risk-based configuration factors and developed a process and MECA/3DAM/CII methodology to measure and evaluate these factors so as to carry out systematic configuration management of mixed used development in urban regeneration projects.

An Analysis of the Elementary Mathematics Textbooks in Singapore: Focused on the Model Method (싱가포르의 초등학교 수학 교과서 분석: 모델 메소드(model method)를 중심으로)

  • Pang, JeongSuk;Kim, EunKyung
    • Education of Primary School Mathematics
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    • v.20 no.3
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    • pp.205-224
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    • 2017
  • A model method has been known as the main characteristic of Singaporean elementary mathematics textbooks. However, little research has been conducted on how the model method is employed in the textbooks. In this study, we extracted contents related to the model method in the Singaporean elementary mathematics curriculum and then analyzed the characteristics of the model method applied to the textbooks. Specifically, this study investigated the units and lessons where the model method was employed, and explored how it was addressed for what purpose according to the numbers and operations. The results of this study showed that the model method was applied to the units and lessons related to operations and word problems, starting from whole numbers through fractions to decimals. The model method was systematically applied to addition, subtraction, multiplication, and division tailored by the grade levels. It was also explicitly applied to all stages of the problem solving process. Based on these results, this study described the implications of using a main model in the textbooks to demonstrate the structure of the given problem consistently and systematically.

Development of Forest Volume Estimation Model Using Airborne LiDAR Data - A Case Study of Mixed Forest in Aedang-ri, Chunyang-myeon, Bonghwa-gun - (항공 LiDAR 자료를 이용한 산림재적추정 모델 개발 - 봉화군 춘양면 애당리 혼효림을 대상으로 -)

  • CHO, Seung-Wan;KIM, Yong-Ku;PARK, Joo-Won
    • Journal of the Korean Association of Geographic Information Studies
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    • v.20 no.3
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    • pp.181-194
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    • 2017
  • This study aims to develop a regression model for forest volume estimation using field-collected forest inventory information and airborne LiDAR data. The response variable of the model is forest stem volume, was measured by random sampling from each individual plot of the 30 circular sample plots collected in Bonghwa-gun, Gyeong sangbuk-do, while the predictor variables for the model are Height Percentiles(HP) and Height Bin(HB), which are metrics extracted from raw LiDAR data. In order to find the most appropriate model, the candidate models are constructed from simple linear regression, quadratic polynomial regression and multiple regression analysis and the cross-validation tests were conducted for verification purposes. As a result, $R^2$ of the multiple regression models of $HB_{5-10}$, $HB_{15-20}$, $HB_{20-25}$, and $HBgt_{25}$ among the estimated models was the highest at 0.509, and the PRESS statistic of the simple linear regression model of $HP_{25}$ was the lowest at 122.352. $HB_{5-10}$, $HB_{15-20}$, $HB_{20-25}$, and $HBgt_{25}-based$ models, thus, are comparatively considered more appropriate for Korean forests with complicated vertical structures.

The Effects of Transformational Leadership on Employees Job Satisfaction & Organizational Identification in Korean Hotel Industry (호텔기업 종사원의 변혁적 리더십이 직무만족과 조직 동일시에 미치는 영향에 관한 연구)

  • Lee, Jun-Hyuk;Kim, Dong-Ki;Park, Ki-Ho
    • Journal of Global Scholars of Marketing Science
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    • v.15 no.2
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    • pp.27-48
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    • 2005
  • This study analyzed the structural elements of transformational leadership on hotel employees job satisfaction and organizational identification, and inquired into how moderating variables such as demographical characteristics and the features of hotels affect transformational leadership. The ultimate purpose of this study was to provide managerial implications to hotel business operators and hotel employees. The main results of this study are as follows; According to the result of factor analysis on transformational leadership and hotel employees job satisfaction, 18 variables were derived as two factors 'obliging leadership' & 'vision leadership' factor in the area of transformational leadership, and 31 variables were derived as four factors 'welfare and work environment', 'ability display and job stability', 'colleague relationship and job performance' and 'company policy' factor. Second, stepwise regression analysis on whether the type of transformational leadership at hotels has a significant effect on employees job satisfaction & organizational identification, 'vision leadership' and job satisfaction and the both 'vision leadership' & 'obliging leadership' among the types of transformational leadership appeared to have a significant effect on hotel employees organizational identification. Third, One-Way ANOVA and t-test in order to examine significant difference in the type of transformational leadership according to demographical characteristics and general characteristics, statistically significant difference was found according to income level, the current position, work experience, the type of hotel operation and the experience of job change.

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User's Emotional Preference on PC OS GUI - Though Semantic Differential Method (PC OS GUI 의 사용자 감성에 관한 연구 - 의미분별 척도법을 활용한 사용자 감성 선호도 분석)

  • Moon, Hyun-Jung;Lee, Jung-Yeun
    • 한국HCI학회:학술대회논문집
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    • 2008.02b
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    • pp.30-35
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    • 2008
  • The purpose of this study is to analyze and define user's emotional satisfaction factors to the PC OS GUI image. The study is to investigate the relationship between PC OS GUI Image and Sensitive Vocabula교 based on user's emotional preference. 47 user preferred sensitive words are collected by the initial survey. Through the similarity test, 47 words are narrowed down to 20 comprehend words. The semantic differential methods is used in the final survey with 5 step questionnaire. From this process, user preferred the GUI design that is vocabularized as Clear, Easy, Safety, Stability. Additionally, the result shows that the image of Clear is related to Safety and the image of Easy is related to Stability. The result of the study could be used in design PC OS GUI as base data.

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Rapid Analytical Method of Volatile- and Semivolatile Organic Compounds in Water and their Monitoring in Water Treatment Plants (물 시료 중 휘발성 및 반휘발성 유기물질들의 빠른 분석법 및 정수처리 단계별 모니터링)

  • Shin, Ho-Sang;Ahn, Hye-Sil
    • Analytical Science and Technology
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    • v.17 no.3
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    • pp.240-250
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    • 2004
  • A gas chromatography-mass spectrometric (GC-MS) assay method was developed for the rapid and sensitive determination of volatile- and semivolatile organic compounds in water. Two hundreds mL of water sample was extracted in a 250 mL separatory funnel with 1 ml of pentane at pH 6.5. Fluorobenzene and 1,2-dichlorobenzene-d4 as internal standards were added to water sample and the solution was mechanically shaken for 5 min and analyzed by GC-MS (selected ion monitoring) without more any concentration or purification steps. The peaks had good chromatographic properties and the extraction of these compounds from water also gave relatively high recoveries with small variations. The range of detection limits of the assay was 0.5-10 ng/L. Turnaround time for up to about 40 samples was one day. This method is simple, convenient, and can be learned easily by relatively inexperienced personnel. This method was used to analyze 15 volatile- and semivolatile organic compounds in water of a Lake, and raw and treated water from three Water Treatment Plants in Korea. As the analytical results, benzene, toluene, xylene, isopropylbenzene, 1,3,5-trimethylbenzene, 1,2,4-trimethylbenzene, naphthalene and 2,4,6-trichlorophenol were detected at concentrations of up to 0.4, 1.9, 1.3, 0.2, 1.8, 13.0, 1.7 and $1.1{\mu}g/L$, respectively. But chlorobenzene, trichloroethylene, tetrachloroethylene, ethylbenzene, n-butylbenzene and dibromochloropropane levels during that period were not significant. The removal effect of the compounds in three Water Treatment Plants was calculated. The compounds studied were generally removed during conventional water treatment, especially during the active carbon filtration.