• Journal of Sericultural and Entomological Science
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• v.52 no.1
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• pp.25-32
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• 2014

We produced the transgenic silkworm that expressed the enhanced blue fluorescent protein (EBFP) in the cocoon of silkworms. The EBFP fusion protein, each with N- and C-terminal sequences of the fibroin H-chain, was designed to be secreted into the lumen of the posterior silk glands. The expression of the EBFP/H-chain fusion gene was regulated by the fibroin H-chain promoter. The use of the $3{\times}P3$-driven DsRed2 cDNA as a marker allowed us to rapidly distinguish transgenic silkworm. A mixture of the donor and helper vector was micro-injected into 300 eggs of silkworms, Baegokjam. We obtained 5 broods. The cocoon displayed blue fluorescence, proving that the fusion protein was present in the cocoon. Also, the presence of fusion proteins in cocoons was demonstrated by SDS-PAGE and western blot analysis. Accordingly, we suggest that the EBFP fluorescence silk will enable the production of the silk-based biomaterials.

• Journal of Sericultural and Entomological Science
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• v.51 no.2
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• pp.153-158
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• 2013

To express green fluorescent protein in the cocoon of silkworm, we constructed the fibroin H-chain expression system to produce enhanced green fluorescent protein (EGFP) in the cocoon of transgenic silkworms. The EGFP fusion protein, each with N- and C-terminal sequences of the fibroin H-chain, was designed to be secreted into the lumen of the posterior silk glands. The expression of the EGFP/H-chain fusion gene was regulated by the fibroin H-chain promoter. The use of the 3xP3-driven DsRed2 cDNA as a marker allowed us to rapidly distinguish transgenic silkworm. A mixture of the donor and helper vector was micro-injected into 1,200 eggs of bivoltin silkworms, Baegokjam. We obtained 8 broods. The cocoon displayed strong green fluorescence, proving that the fusion protein was present in the cocoon. Also, the presence of fusion proteins in cocoons was demonstrated by SDS-PAGE and immunoblotting. Accordingly, we suggest that the EGFP fluorescence silk will enable the production of the novel biomaterial based on the transgenic silk.

• Korean journal of applied entomology
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• v.42 no.4
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• pp.353-360
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• 2003

Immunodepression can be required for entomopathogenic bacteria to induce their potent pathogenicities to the target insects. Here, we raise a hypothesis that the capacity of a pathogenic bacterium to induce the target insect immunodepression has positive relationship with the degree of pathogenicity. X. nematophilus had 1,200 times as potent as another entomopathogenic bacterium, Staphylococcus gallinarum against the fifth instar larvae of silkworm, Bombyx mori, when they were Injected into the hemocoel. Although both bacteria had significant cytotokic effect on the hemocytes of B. mori, X. nematophilus gave faster and greater cytotoxicity than did S. gallinarum. In cellular immune reactions, B. mori could form 20 hemocyte nodules against the bacterial injection with 5${\times}$10$\^$5/ cells. The number of the hemocyte nodules was significantly depressed when live X. nematophilus was inject-ed, but not in S. gallinarum. Activation of prophenoloxidase (proPO) was depressed in the bacterial injection. The depression of PO activation was significantly greater in X. nematophilus infection than in S. gallinarum injection. Lysozyme activity was induced by the injection of S. gallinarum at 4 h after the treatment, but not induced in X. nematophilus at all the time. These results showed that X. nemato-philus induced greater immunodepression against B. mori and resulted in higher pathogenicity than did S. gallinarum. Therefore, this study suggests that the immunodepression induced by entomopathogenic bacteria has positive relationship with their pathogenicity.

• Korean journal of applied entomology
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• v.55 no.1
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• pp.1-9
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• 2016

Edible insects are an interesting alternative global food resource. The purpose of this study was to investigate whether ingestion of silkworm (Bombyx mori) pupae powder with and without resistance exercise training (isometric contraction training) increased muscle mass in ICR mice. To achieve this, 28 ICR mice were grouped into control (CON), resistance exercise training (EX), silkworm pupae powder ingested control (SP), and silkworm powder ingestion with resistance exercise training (SPEX) groups. The change in body weight ratio was significantly decreased in the EX and SPEX groups compared to the CON and SP groups. Total blood protein levels were the highest in SPEX mice compared to those in other groups. The albumin concentration increased only in the EX group. Blood GOT and GPT levels were not significantly affected. Changes in Akt and Gsk-$3{\beta}$ protein expression were not significant but there was a tendency for Akt to increase and for Gsk-$3{\beta}$ to increase following the ingestion of the powder. The size of the gastrocnemius muscle increased significantly in response to resistance exercise training only. Furthermore, the ingestion of silkworm pupae powder tended to increase muscle mass without significance. These results suggested that the ingestion of silkworm pupae powder with resistance exercise training might enhance muscle mass without hepatotoxicity. However, future study may be needed to obtained detailed results and practical suggestions.

• Journal of Sericultural and Entomological Science
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• v.52 no.2
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• pp.159-164
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• 2014

In this study, functionality of products of silkworm hemolymph fermented by Bacillus species was studied such as cell viability, antioxidant effect, and inhibitory effect on tyrosinase activities. A matured silkworm hemolymph was degraded by fermentation with Bacillus subtilis 10854 and Bacillus amyloliquefaciens M27. Especially, proteins of matured silkworm hemolymph were degraded to 3,000 Da by fermentation with B. amyloliquefaciens M27. Cell viability for MTT assay was higher than PBS in hemolymph and fermented hemolymph. A DPPH free radical scavenging activity of fermented hemolymph was as higher as Vitamin C and dependent on sample concentrations. Thus, these results suggest that degraded hemolymph fermented by B. amyloliquefaciens M27 may have antioxidant properties as a material for cosmetics.

• Journal of the Korean Society of Food Science and Nutrition
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• v.30 no.5
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• pp.1004-1009
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• 2001

Acid hydrolysates of cocoon was gained by acid hydrolysis of 2 N HCl, 11$0^{\circ}C$, 48 hours, neutralization and desalting from the cocoon. The amino acid compositions of acid hydrolysates of cocoon were glycine 43.25%, alanine 34.39%, serine 10.05% and valine 2.44%. The contents of essential amino acid was 10.05%. Food efficiency ratio of acid hydrolysates of cocoon group was equal to the reference protein, casein. Liver weight, GOT, GPT activity, serum albumin and serum total protein level of rats were not significantly different among the experimental groups. Therefore, the protein acid hydrolysates of cocoon is not of high quality. When the rat fed with high cholesterol, high lipid, and high sucrose diet was administered with 5% acid hydrolysates of cocoon, its plasma lipids concentration of acid hydrolysates of cocoon was favorably affected: its triglyceride was decreased, and the level of phospholipid and HDL cholesterol were increased. There was also an unfavorable effect: the levels of LDL cholesterol and total cholesterol went up. Therefore, the acid hydrolysates of cocoon is not a good protein food source, but is can be used a cosmetic, medical, or packing material. Further research will reveal how it will affect or improve plasma lipid.

• Journal of Sericultural and Entomological Science
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• v.28 no.1
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• pp.43-53
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• 1986

Among many microbial pesticides, Bacillus thuringiensis is one of the most hopeful pesticide and some commercial products have been appearing on the market. Because these commercial products contain living spores and toxins of the organism, there is a danger that living spores of B. thuringiensis may be scattered by wind and cause a great damage in the sericulture areas. In order to avoide these risks it is desirable to select the strain which has low pathogenicity to the silkworm, and at the sometime being highly pathogenic to the pest insects. Thus this study has been carried out to acquire some basic informations about the procedure of desicable strain selection. Three strains of B. thuringiensis var. kurstaki, var. dendrolimus and var. aizawai were used for the pathogenicity test on the silkworm, Bombux mori and the fall webwarm, Hyphantria cunea. Those strains were investigated by the agarose gel electrophoresis patterns of plasmid DNA determine whether mutation had occured. Pathogenicity tests were carried out of using isolated crystal proteins and spore-crystal protein to mixtures of each strain, seperatively. In case of using spore-crystal protein mixture, the order of pathogenicity in varities of B. thuringiensis against B.mopri and H.cunea were kurstaki, aizawai, dendrolimus and kurstaki, dendrolimus, aizawai, respectively. But using isolated crystal proteins, dendrolimus had the highest toxicity to H. cunea and the lowest toxicity to B. mori among tested three strains. From the above results, dendrolimus was presumed the most desirable straing for using microbial pesticide.

• Journal of Sericultural and Entomological Science
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• v.53 no.2
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• pp.92-96
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• 2015

It is known that silk protein supports effectively proliferation of cell such as insect cell and hybridoma cell. Although there are many varieties of Bombyx mori silkworm, the effect of silkworm varieties on cell proliferation has not been considered in detail. We studied that characteristics of silk cocoon obtained from Baegokjam, Kumokjam, Daeseongjam silkworm varieties and whether silk protein affected cell proliferation or not. Silk sericin was prepared under high temperature and high pressure condition. Silk fibroin was prepared using $CaCl_2:H_2O:EtOH$ with different dissolution time. As a result, there are differences in silk cocoon from different silkworm varieties about cell proliferation. The proliferation was accelerated in the presence of Baegokjam silk sericin and Kumokjam silk fibroin with 5hr dissolution time. We expect that silk proteins could be a preferable culture medium supplement for stimulating the proliferation of cell. Then, this results suggest silk as a new material for medium supplement replacing with fetal bovine serum.

• Proceedings of the Korean Fiber Society Conference
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• 2003.04a
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• pp.275-278
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• 2003

실크 세리신은 누에(Bombyx mori)로부터 얻어지는 천연 거대분자 단백질로서, 누에고치실의 20~30%를 차지하고 있다. 세리신은 외부로의 자극으로부터 피브로인을 보호하는 역할을 하지만, 견사나 견직물을 얻기 위해서는 정련 공정에서 반드시 제거하게 된다. 현재 생산 현장에서는 비누/알칼리 정련법으로 세리신을 제거하고 있는데, 정련 폐액중에 포함되어 있는 세리신 단백질에서 분해된 각종 아미노산과 비누 및 강알칼리 약제로 인해 환경오염의 주된 원인이 되고 있다. (중략)

• The Korean Journal of Zoology
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• v.8 no.2
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• pp.77-79
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• 1965

밤나무산누에나방(Dictyploca japonica)의 아미노산을 paper chromatogrphy법을 이용하여 용에서 난까지 정성적으로 분석하였다. 1. 분석 결과 19 종의 유리아미노산이 검출되었으며 각 stage는 유리아미노산의 정성적 pattern의 변동을 보였다. 2. Serine은 전 stage에서 높은 농도로 검출되었다. 3. 유리아미노산의 정류수는 종류수는 나방이의 발생과 더불어 증가하고 일반적으로 male 보다는 female 에 있어서 그 수가 많았다. 4. Tryptophane은 각 stage에서 전연 검출되지 않았다.