Browse > Article
http://dx.doi.org/10.5656/KSAE.2015.10.0.031

Increased Muscle Mass after Resistance Exercise Training and Ingestion of Silkworm Pupae Powder (Bombyx mori L.) in ICR Mice  

Kim, Yiseul (Dept. of Leisure Sports Kyungpook National University)
Kim, Heebin (Dept. of Leisure Sports Kyungpook National University)
Jeon, Byungduk (Dept. of Physical Education Leisure, Suseong College)
Lee, Dong Woon (Major of Applied Biology, School of Ecological Environment and Tourism, Kyungpook National University)
Ryu, Sungpil (Dept. of Leisure Sports Kyungpook National University)
Publication Information
Korean journal of applied entomology / v.55, no.1, 2016 , pp. 1-9 More about this Journal
Abstract
Edible insects are an interesting alternative global food resource. The purpose of this study was to investigate whether ingestion of silkworm (Bombyx mori) pupae powder with and without resistance exercise training (isometric contraction training) increased muscle mass in ICR mice. To achieve this, 28 ICR mice were grouped into control (CON), resistance exercise training (EX), silkworm pupae powder ingested control (SP), and silkworm powder ingestion with resistance exercise training (SPEX) groups. The change in body weight ratio was significantly decreased in the EX and SPEX groups compared to the CON and SP groups. Total blood protein levels were the highest in SPEX mice compared to those in other groups. The albumin concentration increased only in the EX group. Blood GOT and GPT levels were not significantly affected. Changes in Akt and Gsk-$3{\beta}$ protein expression were not significant but there was a tendency for Akt to increase and for Gsk-$3{\beta}$ to increase following the ingestion of the powder. The size of the gastrocnemius muscle increased significantly in response to resistance exercise training only. Furthermore, the ingestion of silkworm pupae powder tended to increase muscle mass without significance. These results suggested that the ingestion of silkworm pupae powder with resistance exercise training might enhance muscle mass without hepatotoxicity. However, future study may be needed to obtained detailed results and practical suggestions.
Keywords
Silkworm pupae; Resistance exercise; Skeletal muscle; Mouse;
Citations & Related Records
Times Cited By KSCI : 4  (Citation Analysis)
연도 인용수 순위
1 Akpinar. T.S., Tayfur, M., Tufan, F., Sahinkaya, T., Köse, M., Ozşenel, E.B., Bahat, Ozturk, G., Saka, B., Erten, N., Yildiz, S., Karan, M.A., 2014. Uncomplicated diabetes does not accelerate age-related sarcopenia. Aging Male. 17, 205-10.   DOI
2 Amin, H., Vachris, J., Hamilton, A., Steuerwald, N., Howden, R., Arthur, S.T., 2014. GSK3$\beta$ inhibition and LEF1 upregulation in skeletal muscle following a bout of downhill running. J. Physiol. Sci. 64, 1-11.   DOI
3 Camera, D.M., West, D.W., Phillips, S.M., Rerecich, T., Stellingwerff, T., Hawley, J.A., Coffey, V.G., 2015. Protein ingestion increases myofibrillar protein synthesis after concurrent exercise. Med. Sci. Sports Exerc. 47, 82-91.
4 Cheema, B.S., Vizza, L., Swaraj, S., 2014. Progressive resistance training in polycystic ovary syndrome: Can pumping iron improve clinical outcomes? Sports Med. 44, 1197-1207.   DOI
5 Christensen, J.R., Faber, A., Ekner, D., Overgaard, K., Holtermann, A., Sogaard, K., 2011. Diet, physical exercise and cognitive behavioral training as a combined workplace based intervention to reduce body weight and increase physical capacity in health care workers - a randomized controlled trial. BMC Public Health. 27, 671.
6 Chu, P., Gotink, R.A., Yeh, G.Y., Goldie, S.J., Hunink, M.M., 2014. The effectiveness of yoga in modifying risk factors for cardiovascular disease and metabolic syndrome: A systematic review and meta-analysis of randomized controlled trials. Eur. J. Prev. Cardiol. 15, pii: 2047487314562741.
7 Churchward-Venne, T.A., Murphy, C.H., Longland, T.M., Phillips, S.M., 2013. Role of protein and amino acids in promoting lean mass accretion with resistance exercise and attenuating lean mass loss during energy deficit in humans. Amino Acids. 45, 231-240.   DOI
8 DeFoliart, G.R., 1999. Inserts as food: why the western attitude is important. Annu. Rev. Entomol. 44, 21-50.   DOI
9 Desire, D.D., Amelie, M., Claude, B.D., Bibi-Farouck, A.O., Leonard, T., Theophile, D., Pierre, K., 2014. Antihypertensive potential of the aqueous extract which combine leaf of Persea americana Mill. (Lauraceae), stems and leaf of Cymbopogon citratus (D.C) Stapf. (Poaceae), fruits of Citrus medical L. (Rutaceae) as well as honey in ethanol and sucrose experimental model. BMC Complement Altern. Med. 14, 507.   DOI
10 Dickinson, J.M., Gundermann, D.M., Walker, D.K., Reidy, P.T., Borack, M.S., Drummond, M.J., Arora, M., Volpi, E., Rasmussen, B.B., 2014. Leucine-enriched amino acid ingestion after resistance exercise prolongs myofibrillar protein synthesis and amino acid transporter expression in older men. J. Nutr. 144, 1694-1702.   DOI
11 Fang, Z.H., Lee, C.H., Seo, M.K., Cho, H., Lee, J.G., Lee, B.J., Park, S.W., Kim, Y.H., 2013. Effect of treadmill exercise on the BDNF-mediated pathway in the hippocampus of stressed rats. Neurosci. Res. 76, 187-194.   DOI
12 Glass, D.J., 2003. Signaling pathways that mediate skeletal muscle hypertrophy and atrophy. Nat. Cell Biol. 5, 87-90.   DOI
13 Guimaraes-Ferreira, L., Cholewa, J.M., Naimo, M.A., Zhi, X.I., Magagnin, D., Dal, Ponte, de Sa, R.B., Streck, E.L., da Silva, Teixeira, T., Zanchi, N.E., 2014. Synergistic effects of resistance training and protein intake: Practical aspects. Nutrition. pii: S0899-9007(14)00035-5.
14 Jeon, J.R., Park, J.R., 1992. Functional properties of silkworm larvae protein concentrate after enzyme treatments. J. Kor. Soc. Food Sci. Nutr. 21, 706-711.
15 Kim, H., Lee, S., Choue, R., 2011. Metabolic responses to high protein diet in Korean elite bodybuilders with high-intensity resistance exercise. J. Int. Soc. Sports Nutr. 4, 8-10.
16 Kim, J., Kyung, J., Kim, D., Choi, E.K., Bang, P., Park, D., Kim, Y.B., 2012. Anti-obesity effects of rapha diet(R) preparation in mice fed a high-fat diet. Lab. Anim. Res. 28, 265-271.   DOI
17 Kim, T.N., Park, M.S., Ryu, J.Y., Choi, H.Y., Hong, H.C., Yoo, H.J., Kang, H.J., Song, W., Park, S.W., Baik, S.H., Newman, A.B., Choi, K.M., 2014. Impact of visceral fat on skeletal muscle mass and vice versa in a prospective cohort study: The Korean sarcopenic obesity study (KSOS). PLoS One. 17; 9(12), e115407.   DOI
18 Lee, S.H., Park, D., Yang, G., Bae, D.K., Yang, Y.H., Kim, T.K., Kim, D., Kyung, J., Yeon, S., Koo, K.C., Lee, J.Y., Hwang, S.Y., Joo, S.S., Kim, Y.B., 2012. Silk and silkworm pupa peptides suppress adipogenesis in preadipocytes and fat accumulation in rats fed a high-fat diet. Eur. J. Nutr. 51, 1011-1019.   DOI
19 Kitagawa, K., Kotake, Y., Hiramatsu, Y., Liu, N., Suzuki, S., Nakamura, S., Kikuchi, A., Kitagawa, M., 2010. GSK3 regulates the expressions of human and mouse c-Myb via different mechanisms. Cell Div. 21, 5:27.
20 Kruger, K., Gessner, D.K., Seimetz, M., Banisch, J., Ringseis, R., Eder, K., Weissmann, N., Mooren, F.C., 2013. Functional and muscular adaptations in an experimental model for isometric strength training in mice. PLoS One. 8(11), e79069.   DOI
21 Lee, Y.K., Jeon, H.R., Jeon, B.D., Ryu, S., Lee, S.C., 2008. Investigations of body composition, nutritional intake and dietary supplementation status before and after weight loss before match in bodybuilders. Kor. J. Phy. Edu. 47, 467-474.
22 Liu, H.L., Zhao, G., Zhang, H., Shi, L.D., 2013. Long-term treadmill exercise inhibits the progression of alzheimer's disease-like neuropathology in the hippocampus of APP/PS1 transgenic mice. Behav. Brain Res. 256, 261-272.   DOI
23 Moc, C., Taylor, A.E., Chesini, G.P., Zambrano, C.M., Barlow, M.S., Zhang, X., Gustafsson, A.B., Purcell, N.H., 2015. Physiological activation of Akt by PHLPP1 deletion protects against pathological hypertrophy. Cardiovasc. Res. 105, 160-170.   DOI
24 Morris, M.S., Jacquse, P.F., 2013. Total protein, animal protein and physical activity in relation to muscle mass in middle-aged and older Americans. Br. J. Nutr. 109(7), 1294-1303.   DOI
25 Oonincx, D.G., de Boer, I.J., 2012. Environmental impact of the production of mealworms as a protein source for humans - a life cycle assessment. PLoS One. 7(12), e51145.   DOI
26 Myers, T.R., Schneider, M.G., Schmale, M.S., Hazell, T.J., 2014. A whole-body aerobic resistance training circuit improves aerobic fitness and muscle strength in sedentary young females. J. Strength Cond. Res. 5.
27 Oh, H.G., Lee, H.Y., Kim, J.H., Kang, Y.R., Moon, D.I., Seo, M.Y., Back, H.I., Kim, S.Y., Oh, M.R., Park, S.H., Kim, M.G., Jeon, J.Y., Shin, S.J., Ryu, K.S., Chae, S.W., Kim, O., Park, J.K., 2012. Effects of male silkworm pupa power on the erectile dysfunction by chronic ethanol consumption in rats. Lab. Anim. Res. 28, 83-90.   DOI
28 Ohyama, K., Nogusa, Y., Suzuki, K., Shinoda, K., Kajimura, S., Bannai, M., 2015. A combination of exercise and capsinoids supplementation additively suppresses diet-induced obesity by increasing energy expenditure in mice. Am. J. Physiol. Endocrinol. Metab. 308, 315-323.   DOI
29 Petriz, B.A., Almeida, J.A., Gomes, C.P., Pereira, R.W., Murad, A.M., Franco, O.L., 2015. NanoUPLC/MS(E) proteomic analysis reveals modulation on left ventricle proteome from hypertensive rats after exercise training. J. Proteomics. 113, 351-365.   DOI
30 Rumpold, B.A., Schluter, O.K., 2013. Nutritional composition and safety aspects of edible insects. Mol. Nutr. Food Res. 57, 802-823.   DOI
31 Ryu, S., 2014. Silkworm pupae powder ingestion increases fat metabolism in swim-trained rats. J. Exerc. Nutr. Biochem. 18, 141-149.   DOI
32 Seida, A.A., El Tanbouly, N.D., Islam, W.T., Eid, H.H., El Maraghy, S.A., El Senousy, A.S., 2014. Bioassay-guided fractionation of a hepatoprotective and antioxidant extract of pea by-product. Nat. Prod. Res. 10, 1-6.
33 Tipton, K.D. 2011. Efficacy and consequences of very-high-protein diets for athletes and exercisers. Proc. Nutr. Soc. 70, 205-214.   DOI
34 Shi, L., Zhang, H., Chen, Y., Liu, Y., Lu, N., Zhao, T., Zhang, L., 2015. Chronic exercise normalizes changes of cav 1.2 and KCa 1.1 channels in mesenteric arteries from spontaneously hypertensive rats. Br. J. Pharmacol. 172, 1846-1858.   DOI
35 Souza, R.W., Piedade, W.P., Soares, L.C., Souza, P.A., Aguiar, A.F., Vechetti-Junior, I.J., Campos, D.H., Fernandes, A.A., Okoshi, K., Carvalho, R.F., Cicogna, A.C., Dal-Pai-Silva, M., 2014. Aerobic exercise training prevents heart failure-induced skeletal muscle atrophy by anti-catabolic, but not anabolic actions. PLoS One. 9(10), e110020.   DOI
36 Spillane, M., Schwarz, N., Willoughby, D.S. 2014. Heavy resistance training and peri-exercise ingestion of a multi-ingredient ergogenic nutritional supplement in males: effects on body composition, muscle performance and markers of muscle protein synthesis. J. Sports Sci. Med. 13, 894-903.
37 Yang, S.J., Kim, C.Y., Lee, J.B., Kang, S.S., Lee, J.J. 2013. The Effects of the mulberry and silkworm intake on muscle increase of rats for resistance exercise. Kor. Soc. Sericul. Sci. 52, 123-129.
38 Yeo, Y.G., An, E.Y., Ryu, S. 2013. Effect of pupa powder diet on muscle and blood amino acids composition in rat. Kor. J. Phy. Edu. 52, 807-818.
39 Zacharewicz, E., Della, Gatta, P., Reynolds, J., Garnham, A., Crowley, T., Russell, A.P., Lamon, S. 2014. Identification of MicroRNAs linked to regulators of muscle protein synthesis and regeneration in young and old skeletal muscle. PLoS One. 9(12), e114009.   DOI
40 Zhou, J., Han, D. 2006. Safety evaluation of protein of silkworm (Antheraea pernyi) pupae. Food Chem. Toxicol. 44, 1123-1130.   DOI