• Analytical Science and Technology
• /
• v.25 no.5
• /
• pp.313-323
• /
• 2012

We have developed analysis method of PFCs in river water and sediment, and determined seven species of PFCs such as PFBS, PFHxS, PFOS, PFHpA, PFOA, PFNA and PFDA. Water and sediment samples were collected from 21 and 13 different sites along the Nakdong, Seomjin and Nam River, respectively. The water samples were pretreated with HLB cartridge and sediment samples were concentrated after extracted by sonication, and the levels of PFCs were determined by LC-MS/MS. The coefficient of determination ($R^2$) values of calibration curves were higher than 0.99. The method detection limits ranged 0.09~0.63 ng/L in water and 0.013~0.020 ng/g in sediment. The recovery rates of PFCs was found to be 74~98% for water and 87~111% for sediment. PFOA was the major species in water samples and followed by PFHpA and PFOS. In sediment, PFOA, PFOS and PFDA showed similar levels. Both water and sediment samples collected from the Nakdong River showed the highest concentrations of PFCs among the three rivers.

• Journal of the Korea Academia-Industrial cooperation Society
• /
• v.17 no.5
• /
• pp.251-259
• /
• 2016

This study examined the DO concentration distribution and ORP distribution using microbubbles on pilot-scale aeration tanks. As a result of MLSS mixing and oxygen transfer phenomenon using microbubbles, different DO concentrations were observed depending on the circulation of the liquid with the microbubble supply location on the lateral of an aeration tank. The simulation results of CFD (computational fluid dynamics) program showed that MLSS mixed with a microbubble supply in the middle the reactor is much better than on the left side of the reactor. A single reactor containing an anaerobic, anoxic, and aerobic zone, was evaluated without partition according to the location of the microbubble supply based on the experiments and CFD analysis. MLSS was separated into solid-liquid by the microbubble supply in the aeration tank. Consequently, selecting the appropriate microbubble size is important for MLSS mixing and was maintained at the proper DO concentration for biological treatment.

• Journal of Oral Medicine and Pain
• /
• v.38 no.3
• /
• pp.221-233
• /
• 2013

Bile acids are polar derivatives of cholesterol essential for the absorption of dietary lipids and regulate the transcription of genes that control cholesterol homeostasis. Recently it have been identified the synthetic chenodeoxycholic acid (CDCA) derivatives HS-1200 and cisplatin showed apoptisis-inducing activity on various cancer cells in vivo and in vitro. This study was undertaken to investigate the synergistic apoptotic effect of co-treatment with HS-1200 and cisplatin on human tongue squamous cell carcinoma cells (SCC25 cells). To investigate whether the co-treatment with HS-1200 and cisplatin compared to each single treatment efficiently reduces the viability of SCC25 cells, MTT assay was conducted. The induction and augmentation of apoptosis were confirmed by DNA electrophoresis, Hoechst staining and an analysis DNA hypoploidy. Westen blot analysis and immunofluorescent staining were also performed to evaluate the expression levels and the translocation of apoptosis-related proteins following this co-treatment. Furthermore, proteasome activity and mitochondrial membrane potential (MMP) change were also assayed. In this study, co-treatment with HS-1200 and cisplatin on SCC25 cells showed several lines of apoptotic manifestation such as nuclear condensations, DNA fragmentation, reduction of MMP and proteasome activity, the increase of Bax and the decrease of Bcl-2, decrease of DNA content, the release of cytochrome c into cytosol, translocation of AIF and DFF40 (CAD) onto nuclei, and activation of caspase-9, caspase-7, caspase-3, PARP and DFF45 (ICAD) whereas each single treated SCC25 cells did not show these patterns. Although the single treatment of $25{\mu}M$ HS-1200 and $4{\mu}g/ml$ cisplatin for 24 h did not induce apoptosis, the co-treatment of these reagents prominently induced apoptosis. Therefore our data provide the possibility that the combination therapy with HS-1200 and cisplatin could be considered as a novel therapeutic strategy for human squamous cell carcinoma.

• Korean Journal of Environmental Agriculture
• /
• v.18 no.4
• /
• pp.321-326
• /
• 1999

This study was performed to evaluate the quality of produced compost and to analyse the change of a component during the reduction compost according to the input volume. The volume of pilot scale used in this study was about 300㎥. The pile of 2m width, 20m length and 1.2m height was constructed. Woodchip was used as bulking agent to enhance pore volume of composting bay and to control water content of food waste in starting time. Food waste was turned using a mechanical tumer twice a day. The result are as follows : In these cases of input volume of $2m^3$ and $3.5m^3$, temperature of composting pile was maintained over $60^{\circ}C$ and water content was 43.6% and 47.2%, respectively. It was proved that microorganisms activity was maintained high in each input volume. After operation of step 1 and step 2, pH and organic matter in the final compost were 6.2, 6.6 and 84.3%, 79.6%, respectively. Cation concentration such as $K_2O,$ CaO and NaCl was accumulated in the compost during the composting period. NaCl concentration in the final compost was 4.62%, 4.92%, respectively. Hence, If was recommended that this compost should be applied to others expect agricultural area or mixed with a low concentration other compost. In the steps 1, input volume of $2m^3$, heavy metal concentration of Pb, Cu, Cr, Ni. Cd were 37.82㎎/㎏, 56.87㎎/㎏, 9.8㎎/㎏. 22.21㎎/㎏ and 3.69㎎/㎏, and 44.55㎎/㎏, 95.54㎎/㎏, 12.22㎎/㎏, 24.94㎎/㎏, and 3.86㎎/㎏ in the step 2.

• Journal of Biosystems Engineering
• /
• v.9 no.1
• /
• pp.62-70
• /
• 1984

가축배설물(家畜排泄物)의 콤포스트화(化) 작업(作業)의 공학적(工學的)인 국면(局面)은 축산(畜産) 경영(經營)의 축산물(畜産物) 생산성(生産性), 에너지 보전(保全), 공해방지(公害防止)와 분뇨처리(糞尿處理)의 노력(勞力) 절약(節約)에 긴밀(緊密)한 관계(關係)가 있다. 그러나 우리나라에 있어서 축산업(畜産業)의 사양(飼養) 규모(規模)와 두수(頭數)는 매년(每年) 증가(增加)하고 있는 경향(傾向)인바 전통적(傳統的)인 가축(家畜) 배설물(排泄物) 처리(處理) 및 재이용(再利用) 과정(科程)에서 발생(發生)되는 문제점(問題點)에 대(對)한 연구(硏究) 자료(資料)가 없고 특(特)히 콤포스트화(化) 작업(作業)이 원시적(原始的)이므로 개선(改善) 확립(確立)이 절실(切實)히 요구(要求)되는 실정(實定)이다. 본(本) 연구(硏究)의 목적(目的)은 가축(家畜) 분뇨(糞尿)의 콤포스트화(化) 작업(作業) 과정(科程)에서 공학적(工學的)인 제(諸) 문제점(問題點)을 조사(調査) 분석(分析)하여 현상(現狀)을 평가(評價)하고 개선(改善) 방안(方案)을 모색(摸索)하기 위한 기본자료(基本資料)를 제공(提供)하고져 수행(遂行)하였다. 이에 관한 조사(調査)와 분석(分析) 결과(結果)를 요약(要約)하면 다음과 같다. 1. 축산농가(畜産農家)의 가축(家畜) 배설물(排泄物) 처분(處分) 방법(方法)은 대부분(大部分)이 자연건조(自然乾燥), 퇴적(堆積), 혐기적(嫌氣的)인 고형(固形) 또는 액상(液狀) 퇴비화(堆肥化)에 의하여 실시(實施)되고 있었으며 가축분뇨(家畜糞尿)로 인(因)한 공해(公害)는 심각(深刻)하다는 것을 알 수 있었다. 2. 호기성(好氣性) 야적(野積) 콤포스트의 통기작업(通氣作業)은 트랙터에 부착(附着)되어진 전후(前後) 작동식(作動式) 로우더가 바람직하다. 3. 농축산(農畜産) 폐기물(廢棄物)의 콤포스트화(化) 작업(作業)은 축산농가(畜産農家)를 중심(中心)으로 한 콤포스트 센타에서 전체 농가(農家)가 지방(地方) 증진(增進)과 오염(汚染) 방지(防止)를 하기 위해 참여(參與)하여 완전(完全) 공동(共同) 이용(利用) 체계에 따라 일관성(一貫性)있게 수행(遂行)되어야 한다. 4. 호기성(好氣性) 야적(野積) 콤포스트 작업(作業)은 다른 방법(方法)에 비하여 운영비(運營費)가 적게 들어 영세적(零細的)인 축산업(畜産業)의 경우(境遇)에 가장 적당(適當)하고 공해(公害)를 방지(防止)함으로서 한국(韓國) 축산농가(畜産農家)의 콤포스트화(化) 시설(施設)로 전망(展望)이 밝다고 판단(判斷)된다.

• KSBB Journal
• /
• v.21 no.4
• /
• pp.312-316
• /
• 2006

A optimal condition for the Ginkgo biloba extraction in ethanol and water binary solvent system has been proposed based on concentration of bilobalide and ginkgolide known as having a antimicrobial components in the range 5% to 70% ethanol in water at $80^{\circ}C$. Concentration of bilobalide as a single component of Ginkgo biloba leaves extract is the highest at the 60% ethanol and ginkgolide A and B is highest at 50% ethanol. Antimicrobial effect of Ginkgo biloba leaves extracts on the S. aureus was also examined by disc diffusion test and optical density test. In case of the disc diffusion test, the clean zone diameter was increased from 0.95 cm to 1.70 cm as ethanol concentration increased from 5 to 70%. However, over the 40% of ethanol concentration the antimicrobial effect was almost flat. Based on these results, we propose that the 40% of ethanol and 60% water solvent is most desirable for Ginkgo biloba extract considering vapor pressure problem in concentrating process after extraction. We introduced SEM and TEM to figure out the morphological change on the surface and inside body of S. aureus when Ginkgo biloba leaves extract was treated. After mixed with Ginkgo biloba leaves extract blast like blebs appeared on the surface of S. aureus cells and cell wall was not observed. From the these results, it seems that the Ginkgo biloba leaves extract including bilobalide and ginkgolide A, B prevent cell wall synthesis.

• Weed & Turfgrass Science
• /
• v.6 no.3
• /
• pp.212-221
• /
• 2017

An actinomycetes isolate G-0299 obtained from a forest soil showed strong phytotoxic activity to Digitaria ciliaris. For the foliar application study, the culture filtrate of the isolate G-0299 showed strong herbicidal activity only to D. ciliaris among the 12 monocot and 5 dicot weed species. And herbicidal activity at a concentration of 500, 250, 125 and $62.5{\mu}gmL^{-1}$ of culture filtrate was 100%, 98%, 70% and 40%, respectively. Phytotoxic symptoms of the culture filtrate by foliar application were desiccation and burn-down or bleaching of leaves and finally plant death. And then the herbicidal activity was exhibited only under the light condition. Also, chlorophyll loss of D. ciliaris leaf tissues in the light condition was much higher than in the dark condition and then chlorophyll content decreased 82%, 5%, respectively. In conclusion, our results suggest that soil bacteria, isolate G-0299 could be a good candidate for new bio-herbicide and provide a new lead molecule for a more unique herbicide.

• KSBB Journal
• /
• v.22 no.4
• /
• pp.201-206
• /
• 2007

The demand of L-arabinose has been increased recently because of its advantages including clinical effect. L-arabinose can be produced from dilute acid hydrolysis of agricultural wastes. In this study, optimum conditions of L-arabinose production using dilute acid hydrolysis of agricultural wastes and nutshells were determined. Among the tested various agricultural wastes and nutshells, corn fiber was selected as the best raw material for the production of arabinose. The highest arabinose production was achieved an acid hydrolysis of corn fiber for 1 h at 130$^{\circ}C$ with 0.4% sulfuric acid. Above optimal conditions, it was obtained 20.1 g/L glucose, 10.1 g/L xylose, 7.8 g/L arabinose and 1.8 g/L galactose from 90 g/L of corn fiber. For the purification of arabinose, it was carried out to remove all of sugars except arabinose by the Candida tropicalis cultivation of acid hydrolyzate and an organic contaminants such as pigments by the active carbon treatment of fermentation broth. Moreover, experiments were carried out to eliminate an ions by exchange chromatography. Finally, we obtained 3.1 g of partially purified L-arabinose powder with about 40% yield by evaporation and vacuum drying.

• Clean Technology
• /
• v.18 no.2
• /
• pp.209-215
• /
• 2012

The performance and removal efficiencies of a pilot scale biofilter were estimated by using ammonia and hydrogen sulfide as the odorous gases. Expanded polyurethane foam coated with powdered activated carbon and zeolite was used as a biofilm supporting medium in the biofilter. Odorous gases from the sludge thickener of a municipal wastewater treatment plant were treated in the biofilter for 10 months and the inlet ammonia and hydrogen sulfide concentrations were 0.1-1.5 and 2-20 ppmv, respectively. The removal efficiencies reached about 100% at the empty bed retention time (EBRT) of 3.6-5 seconds except for the adaptation periods. The pressure drop of the biofilter caused by the gas flow was also low that the maximum attained was 31 mm $H_2O$ during the operation. Its stability was confirmed in the long term due to the fact that the biofilter and the polyurethane medium had a minimum plugging and compression. The microbial community on the medium is critical for the performance of the biofilter especially the distribution of ammonia oxidizing bacteria (AOB) and sulfur oxidizing bacteria (SOB). The distribution of Nitrosomonas sp. (AOB) and Thiobacillus ferroxidans (SOB) was confirmed by FISH (fluorescence in situ hybridization) analysis. The longer the operation time, the more microbial population observed. Also, the medium close to the gas inlet had more microbial population than the medium at the gas outlet of the biofilter.

• Korean Journal of Food Science and Technology
• /
• v.30 no.6
• /
• pp.1339-1344
• /
• 1998

To develop natural flavoring substances, optimal hydrolysis conditions for two stage enzyme hydrolysates (TSEH) using small kingfish (Maegari) were investigated. The optimal conditions for TSEH were revealed in temperature at $50^{\circ}C$ for 3 hours digestion with alcalase (Aroase AP-10, pH 8.0) at the 1st stage and 2 hours digestion at $45^{\circ}C$ with neutrase (Pandidase NP-2, pH 6.0) at the 2nd stage. From the results in quality tests of water extracts, autolytic extracts and 4 kinds of enzyme hydrolysates, TSEH processing method was superior to other methods on the aspects of yield, nitrogen contents, taste such as umami intensity and inhibition of off-flavor formation, and transparency of extracts. We may conclude that TSEH from small kingfish was more flavorable compared with the conventional seasoning materials, it could be utilized as the seasoning substances for fisheries processing.