• Korean Journal of Food Science and Technology
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• v.30 no.2
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• pp.446-449
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• 1998

Dental caries suppression effect and other physiological properties of erythritol were measured in comparison with those of sucrose, xylitol and sorbitol. The susceptibility test for dental caries by using Streptococcus mutans KCTC 3065 and Lactobacillus acidophillus indicated that erythritol was as effective as xylitol in suppressing dental caries. In lactic acid fermentation test, erythritol showed the least growth of bacteria among the tested sweeteners. The tolelerance test by using mice showed that diarrhea began by feeding once 1500 mg erythritol/kg b.w., 1500 mg xylitol/kg b.w. and 1000 mg sorbitol/kg b.w. respectively.

• The Korean Journal of Malacology
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• v.24 no.1
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• pp.59-66
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• 2008

The ark shell, Scapharca broughtonii were collected from the Jinhae bay in Kyungnam, Korea from April 2006 to October 2007. To assess how much they have tolerance against hypoxia under the compound condition of temperature and salinity, survivorship, oxygen consumption rate and external features of Scapharca broughtonii were measured, The 7 days-$LC_{50}$ of dissolved oxygen (DO) for Scapharca broughtonii at $15^{\circ}C$-ordinary sea water and $26{\pm}1$ psu was 1.98mg/L (confidence limit: 1,45-2.39 mg/L) and 2.63mg/L (confidence limit: 1.76-3.37mg/L) respectively. Whereas 3 days-$LC_{50}$ at $25^{\circ}C$-ordinary sea water was 3.89mg/L (confidence limit: 3.36-4.55 mg/L) and 4 days-$LC_{50}$ at $25^{\circ}C-26{\pm}1$ psu was 3.74mg/L (confidence limit: 3.19-4,43 mg/L). Oxygen consumption rate with each experimental group was decreased during the period exposed to hypoxia. To recovery, they were replaced to ordinary and oxygen consumption rate was increased a little, but all died, The color of the umbo of shell in Scapharca broughtonii was changed dark during hypoxia. These data will provide important fundamental information for examining the causes of mass mortality of shellfish in the summer.

• Journal of Food Hygiene and Safety
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• v.23 no.2
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• pp.121-128
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• 2008

The aim of this study was to develop a LightCycler-based real time PCR (LC-PCR) assay and to evaluate its diagnostic use for the detection of Staphylococcus aureus in raw milk samples. Following amplification of 113 bp of coa gene encoding an coagulase precursor specific for Staphylococcus aureus, melting curve and DNA sequencing analysis was performed to verify the specificity of the PCR products. Amplification of 209 bp gene encoding an altered penicillin-binding protein, PBP2a (mecA), melting curve analysis and DNA sequencing analysis was performed to verify methicillin resistance Staphylococcus aureus (MRSA). According to this study, 6 of 647 raw milk samples showed S. aureus positive and 2 of them showed a mecA positive and the detection limit was 10 fg of DNA. And we also isolated Staphylococcus chromogenes a causative agent of exudative epidermitis in pigs and cattle from 3 samples.

• 한국생물공학회:학술대회논문집
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• 2000.04a
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• pp.374-377
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• 2000

A strain of Pseudomonas sp. BCNU 154 was isolated from contaminated soil with orangic solvents as the sole source of carbon and energy. It utilized an exceptionally wide aromatic substrates. The strain BCNU 154 was able to utilize toluene, p-xylene, ethylbenzene, cumene, as the only carbon and energy source. When toluene or p-xylene was used as the sole carbon and energy source, the compound was rapidly degraded with significant increase in biomass concentruction. The biodegradation of this compound was observed when ethylbenzene or cumene was supplied on the carbon source and energy source, which may be a candidate extremophilic bacterium for the bioremediation technology.

• Journal of Marine Life Science
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• v.8 no.1
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• pp.50-55
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• 2023

Probiotics have been isolated from various environments and Bacillus species are advantageous among the probiotic bacteria due to their ability to form endospores that can compensate for the limitation of typical probiotics such as lactic acid bacteria. The aim of this study is to investigate the probiotic potential of Bacillus species from Ulleungdo soil, known as an unpolluted environment in Korea. Soil samples were collected from various areas of Ulleungdo, and Bacillus spp. were isolated, and assessed for antibiotic resistance and enzymatic activity. Six Bacillus spp. were not resistant to all tested antibiotics and subsequently tested for enzyme activity. We found the six Bacillus spp. were all inactive β-glucuronidase enzyme, which can have detrimental effects on human health, and one of Bacillus spp. showed an activity of Leucine arylamidase suggesting its probiotic potential.

• Journal of Korean Traditional Oncology
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• v.23 no.1
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• pp.23-32
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• 2018

Objective: The purpose of this study is to report the case of a patient with non-small cell lung adenocarcinoma (NSCLC) with anaplastic lymphoma kinase (ALK) mutation treated by Samchilchoongcho-Jung in conjunction with Crizotinib. Methods: An NSCLC patient diagnosed with multiple pleural and lymph nodes metastasis has been taking Crizotinib (500 mg/day) since January 2014. The patient has been treated with Samchilchoongcho-Jung (1,500 mg/day) since June 2014. The tumor size was measured by computed tomography (CT) and laboratory analysis was conducted. Adverse events were evaluated by the National Cancer Institute Common Terminology Criteria for Adverse Events (NCI-CTCAE), version 5.0. Results: After combining treatment, stable disease was shown on CT. The tumor marker (CEA, Cyfra 21-1) levels were maintained. NCI-CTCAE 5.0 showed no adverse events. Conclusion: This case study suggests that Samchilchoongcho-Jung may contribute to tumor response, in conjunction with Crizotinib on the treatment of patients with NSCLC.

• Journal of the Korean Chemical Society
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• v.57 no.1
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• pp.81-87
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• 2013

Staphylococcus aureus is the most important pathogen in nosocomial infections, including bloodstream infections. Prompt identification of S. aureus from blood cultures and detection of methicillin resistance are essential in cases of suspected sepsis. We have studied a new method for the sequence-specific visual detection of minute amounts of nucleic acids using intercalating reaction by addition of SYBR Green to amplicons of LAMP, and it's a unique gene amplification method in which DNA can be isothermally amplified using only one enzyme. Staphylococcus-LAMP, which targets the spa gene, encoding S.aureus-specific protein A, and the mecA gene, encoding penicillin-binding protein-2' for methicillin resistance, detected MRSA and MRSE. In this study, by using LAMP assay, I detected for Staphylococcus aureus and Staphylococcus epidermidis concentration in the clinical sample. The detection of Staphylococcus aureus and Staphylococcus epidermidis was tested by using serial 10-fold dilutions standard solution. I have accurate detected the limit of detection, sensitity, specificity and reproducibility of the assay. The Bio-chip based LAMP assay allowed easy, rapid, accurate and sensitive detection of infection with Staphylococcus and especially applicable in a resource-limited situation.

• Journal of Broadcast Engineering
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• v.5 no.2
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• pp.220-226
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• 2000

A novel audio watermark algorithm, the Modified Patchwork Algorithm, is applied to the speech to show that it is effective for digital broadcasting systems. Digital broadcasting system does not separate speech from audio data. However. speech data is very important especially for educational broadcasting. Speech can carry more information than video data. Thus, intellectual property management and protection for speech data is urgent. This paper addresses the technical issues, speech watermark algorithm, and its robustness against malicious attacks.

• Journal of the korean veterinary medical association
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• v.34 no.8
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• pp.566-569
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• 1998

병원성대장균에 의한 자돈의 대장균증 중 대표적인 것이 신생 및 포유자돈에서 주로 발생하는 설사병과 이유자돈의 부종병을 들 수 있다. 이러한 자돈의 대장균증은 돼지유행성설사병(PED)이나 전염성위장병(TGE)처럼 폭발적인 발생을 하지는 않지만 지역이나 계절에 관계없이 광범위하게 지속적으로 발생하고 있기 때문에 전체적인 피해는 오히려 폐사율은 높으나 주기적이고 산발적인 발생을 하는 바이러스성 설사병보다도 많을 것으로 추정된다. 이러한 자돈의 대장균설사증 대책으로 몇종의 백신이 사용되고 있으나 완벽한 장관면역을 형성시켜주는 예방약은 아직 세계적으로도 개발되어 있지 않은 실정이고, 치료대책으로 사용하고 있는 항생제도 내성균 확산으로 치료제 사용이 한계에 이르고 있다. 더욱이 부종병의 경우 치료 및 예방대책이 없어 양돈농가로서는 속수무책인 실정이다. 수의과학연구소에서는 이러한 문제점을 해결하기 위하여 대장균증을 동시에 치료, 예방할 수 있는 병원성대장균에 대한 난황항체를 개발하여 실험실 및 야외적용시험을 실시한 결과 대장균설사증은 물론 부종병치료에도 탁월한 효과를 나타내었기에 소개드리고자 한다.

• Korean Journal of Microbiology
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• v.25 no.3
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• pp.229-237
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• 1987

To determine the characteristics of heterotrophic bacterial community in estuarine ecosystem, water and sediment samples were taden from Naktong estuary. All isolates were compared with 73 characters and described by cluster analysis. With same characters, 30 reference strains were able to divide into approximate species level at 80% similarity (S value). Diversity indices ($H^{1}$) of sediment column isolates were higher than water column isolates. The bacterial community commonly appeared in water and sediment column was reduced with going to downstream. Tolerance indices for temperature (Pt) and salinity (Ps) were also higher in sediment isolates than in water isolates. The bacterial community in sediment column is believed to be composed with diverse populations compared to water column and maintains its stability against various environmental changes with high physiological tolerances.