• The Journal of Korean Association of Computer Education
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• v.15 no.2
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• pp.47-55
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• 2012

We discovered the fact that the most important factor for judging adults' internet addiction in the K-scale method which was developed by Korea National Information Society Agency (NIA), has composed of 4 categories including 20 items, is tolerance and preoccupation factor from the experiments by using data mining techniques. In this research, we propose a new internet addiction diagnostic method based on the degree of tolerance considering users' non-duty internet activities. From some questionnaire participants, their feedbacks for the K-scale and the proposed diagnostic method were collected, and then we confirmed that the proposed user-centered diagnostic method is effective to find undiscovered addicts due to individuals's intention in the K-scale.

• Journal of Yeungnam Medical Science
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• v.14 no.1
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• pp.67-76
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• 1997

The occurrence of multidrug resistance (MDR) is one of the main obstacles in the successful chemotherapeutic treatment of cancer. In this study The gene expressions of multidrug resistance-associated protein (MRP), c-myc and c-fos were investigated in L1210 cells. Adriamycin- or vincristine-resistant L1210 cells, L1210AdR or L1210VcR, respectively, has been identified to overexpression of mdr1 gene. The expression leve of MRP gene in L1210AdR and L1210Cis was more decreased than that in L1210 cells. The c-myc and c-fos genes were expressed both in L1210 and resistant sublines. In L1210AdR, the expressions level of c-myc and c-fos genes were decreased than in L1210. However, in L1210VcR and L1210Cis, c-myc and c-fosgene expressionwere rather increased than L1210. These results suggested that MRP does not contribute in resistance of drug-resistant L1210 cells and there is no relations between MRP and mdr1 gene expression. The expression of c-myc and c-fos gene may be changed during transformation of L1210 to drug-resistant sublines.

• Tuberculosis and Respiratory Diseases
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• v.59 no.6
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• pp.619-624
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• 2005

Background : The drug resistance rate in tuberculosis patients with history of chemotherapy is an important indicator of for evaluation of appropriateness of treatment regimens and compliance of patients. This study examined the long-term changes in the drug resistance rates among TB patients failed in treatment or reactivated. Methods : The results of drug susceptibility testing data from patients registered in health centers from 1981 to 2004 were analyzed. Results : The rate of resistance to isoniazid decreased from 90% to 20%, and the resistance to ethambutol decreased from 45% to 6%. The rate of resistance to rifampicin varied from 13% to 28% and the resistance to pyrazinamide was 5% to 10%. Multidrug resistance was about 2-3% lower than any rifampicin resistance rates. The second-line drug resistance was ranged from 1% to 3%. There was no difference between patients' genders. Patient numbers per 100,000 population increased with age. The regional distribution was even at 4-6 patients per 100,000 population, and drug resistance rates were significantly lower in big city areas than in small towns and rural areas. Conclusion : The rates of resistance of Mycobacterium tuberculosis isolated from TB patients with history of chemotherapy to isoniazid, rifampin, ethambutol, and isoniazid plus rifampin were significantly decreased during over two decades.

• Journal of Yeungnam Medical Science
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• v.9 no.1
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• pp.75-89
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• 1992

The development of multi drug-resistant tumor cell population is a major problem in the chemotherapy of human cancer. These cells are often cross resistant to unrelated drugs and the precise mechanisms of multidrug resistant phenotype of tumor cells has not been fully elucidated. Cisplatin resistant tumor cell(SNU-1/$Cis_5$) was induced from human stomach cancer cell line(SNU-1) in vitro. Growth profiles of survival cells were observed during 5 days by thiazolyl blue (MTT) assay. To investigate the cross resistance of various anticancer drugs in SNU-1 and SNU-1/$Cis_5$, We compared the value of $IC_{50}$ - drug concentration at 50% survival of control and gained relative resistances (RR). The RR for SNU-1/$Cis_5$ were as follows; vinblastine, > 43.0 ; epirubicin, 22.9 ; dactinomycin, 16.0 ; etoposide, 15.0 ; vincristine, 9.2 ; adriamycin, 5.7 ; aclarubicin, 5.3. But 5-fluorouracil, methotrexate, daunorubicin have not cross resistance with cisplatin. Resistant inhibition values of $10{\mu}M$ verapamil for SNU-1/$Cis_5$ were as follows; vincristine, 13.1 ; epirubicin, 10.0 ; etoposide, 6.3 ; vinblastine, 4.4 ; dactinomycin, 3.6 ; daunorubicin, 2.4. Membrane proteins of 51,400 and 81,300 daltons were identified by radioiodination with SDS-PAGE, which might represented the drug resistance.

• Korean Journal of Plant Tissue Culture
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• v.28 no.1
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• pp.15-20
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• 2001

Protoplast and callus of three rice varieties, CI5309 (salt-tolerant), Dongjinbyeo (moderately tolerant), Nakdongbyeo (salt-sensitive), were cultured on the MS medium containing 0, 0.3, 0.6, and 0.9% of NaCl. The survival rates of protoplasts were decreased according to the hours after NaCl treatment, and the survival rates of protoplast differed among three varieties at 9∼l2 hours after treatment. The contents of proline of CI5309 and Dongjinbyeo were almost the same at the different levels of NaCl, and that of Nakdongbyeo, however, was greatly decreased at the high concentration of NaCl. The contents of Ca, K and Mg were decreased according to the increasing of NaCl concentrations, but the contents of those did not differ among three varieties at the high concentration of NaCl. The contents of Na in each varieties were almost the same at the three levels of NaCl.

• Proceedings of the Korean Society of Crop Science Conference
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• 1993.10a
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• pp.40-41
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• 1993

• Korean Journal of Food Science and Technology
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• v.33 no.5
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• pp.582-588
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• 2001

To control the growth of Salmonella on raw chicken supplied in food service, they were isolated and analysed for their physiological characteristics. Total viable microbe counts under the skin of the chicken amounted to 10% of numbers on their skin. Fifty one of Salmonella were isolated and identified from 75 chicken carcass samples. About 70% of the isolates showed resistance to more than four antibiotics, which indicated very high resistance among the strains ever reported in Korea. Lactic acid and trisodium phosphate(TSP) as sanitizing agent were applied to the Salmonella spp. isolates by agar diffusion method. The resistance by isolates to those sanitizing agents was compared with type strains of S. typhimurium ATCC 14028, S. typhimurium ATCC 12023, S. heidelberg and S. enteritidis. The isolates showed similar or less resistance to the type strains as for the lactic acid. However, as for trisodium phosphate known recently as a good sanitizer, 50% of the isolates showed more resistant compared with the type strains. Also, the treatment time and regeneration medium affected significantly the resistance of the isolates, which indicates that more careful test might be needed for TSP's practical application to raw chicken. Therefore, From the results, it is suggested that antibiotic treatment during breeding broiler and contamination after slaughtering should be minimized along with careful use of sanitizing agent in order to ensure more safe raw chicken supply.

• Proceedings of the Korean Society of Food Hygiene and Safety Conference
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• 2002.05a
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• pp.166-169
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• 2002

유전자재조합(GM)식품에 대해 듣거나 읽어서 알고 있는 사람이 67%정도 되었고,GM식품을 자발적으로 먹겠다는 사람은 18%를 약간 상회하였고 기다려보고 결정하겠다는 사람들이 약 50%정도였다. 농약 등의 화학물질 오염이나 취급부주의 및 기업인의 윤리의식에 대한 불신에 비해 GM식품에 대한 우려는 매우 적게 나타났으나 GM방법에 의해 개발한 제초제 내성 콩을 먹겠다는 사람은 적은 데 비해 같은 방법으로 개발한 비타민이 많은 콩을 먹겠다는 사람은 많은 것으로 보아 소비자들은 GMO이기 때문에 제초제 내성 콩을 거부하는 것이 아니라, 제초제 내성 콩에 대한 내용의 이해부족으로 인한 거부감을, 그리고 비타민 콩은 비타민이 인체에 중요한 영양소라는 것 때문에 호감을 가지는 것으로 해석되었다. 전체적으로 소비자들이 GMO에 관한 정보를 많이 접하기는 하지만 보통 식품에도 유전자가 있다는 사실을 아는 소비자들이 조사 대상자의 40%정도인 것으로 보아 노출된 만큼 내용을 잘 알지 못하는 것으로 나타났다. GM식품에 대하여는 90%이상이 표시하기를 바라고 있는 것과 같이 2001년 7월부터 가공식품에 GM원료 함유여부 표시제도가 시행되고 있는 데, 이 제도의 시행 사실을 알고 있는 소비자는 22%에 지나지 않았다. 효과적인 표시제도의 시행을 위해서는 일반시민이 제도의 시행에 대해 잘 숙지하고 있어야 할 것이다.

• Journal of fish pathology
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• v.12 no.2
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• pp.115-121
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• 1999

MIC test of 16 chemotherapeutic agents was performed on 24 isolates of Edwardsiella tarda collected from flounders. They revealed resistance against combinations of ampicillin, amoxicillin, erythromycin, flumequine, doxycycline(DOXY), nalidixic acid, novobiocin, oxolinic acid, oxytetracycline(OTC), thiamphenicol(TP) and sulfonamide. Two strains carried transferable R plasmid encoding Otc Kanamycin Tp and Otc chloramphenicol Doxy Tetracycline Tp, respectively. The R plasmids were not similar each other on the basis of their digestion pattern of restriction endonuclease, suggesting distribution of different transferable R plasmid among E. tarda from flounders.

• Korean Journal of Environmental Agriculture
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• v.15 no.3
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• pp.316-324
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• 1996

The four tolerant and one susceptible plant to oxyfluorfen were selected from 26 species and investigated for the inhibition of protox activity, the PPIX accumulation, and the activity of antioxidative enzymes by oxyfluorfen treatment. When treated, the oxyfluorfen-tolerant plant species showed a less decrease in fresh weight and height than the susceptible one. The susceptible barnyardgrass showed more inhibition of protox activity due to the treatment of oxyfluorfen than the tolerant species. Especially at the concentration of $10^{-6}M$, protox activity of the susceptible barnyardgrass was inhibited completely, but tolerant species maintained $25{\sim}45%$ of the activity. Under the light and dark condition, the susceptible barnyardgrass showed more PPIX accumulation than the tolerant.