• The Korean Journal of Cytopathology
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• v.1 no.2
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• pp.152-157
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• 1990

A 70-year-old female who was diagnosed as myxoid chondrosarcoma by fine needle aspiration of a pleural mass is described. She presented with left chest discomfort of 4 months' duration and aggravating dyspnea and chest pain for 2 months. Chest X-ray and CT scan revealed a large lobulated low density mass invading chest wall at the left pleural cavity and massive pleural fluid. Fine needle aspiration was done under the impression of mesothelioma or metastatic cancer. The aspirates from the mass were very cellular and composed of isolated or clustered forms of large plump cells. Abundant cytoplasm was blulsh opaque and the margin was rounded in the isolated cells, whereas clustered cells show ill-defined ceil borders and aggregating tendency. The nuclei were eccentric, round to ovoid, and had fine chromatin pattern and multiple small nucleoli. Cellular pleomorphism or mitotic figure was not definite. These findings were consistent with cytologic features of chondrosarcoma. Final diagnosis was confirmed as myxoid chondrosarcoma by mediastinoscopic biopsy and the tumor showed strong positivity for S-100 protein.

• Development and Reproduction
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• v.7 no.2
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• pp.127-136
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• 2003

The present study was conducted to investigate gene expression profile of mouse ovaries during the primordial-primary follicle transition. We isolated total RNA from mouse ovaries at day1(contains only primordial follicles) and day5(contains both primordial and primary follicles) and synthesized cDNA using annealing control primers(ACP, Seegene, Inc., Seoul, Korea). Using 80 different ACPs for PCR, we cloned, sequenced, and analyzed identities of 41 differentially expressed genes(DEGs). According to BLAST analysis, sequences of 33 clones significantly matched database entries, 4 clones were novel, and 4 clones were ESTs. We selected 8 DEGs with interesting functions, Anx11 and Pepp2-Pending highly expressed in day1 ovary, while Apg3/Autlp-like, BPOZ, Ches1, Kcmf1, NHE3, Nid2, Ninj1, SENP3, Suil-rsl, and TIAP/m-survivin highly expressed in days ovary, and confirmed their different expression between day1 ovaries and days ovaries using semi-quantitative RT-PCR. There was no false positive result. Using in situ hybridization, we found that almost all of genes studied were expressed in the oocyte from primordial follicle stage but expression decreased from primary follicle stage. Meanwhile their expression was increased in cuboidal granulosa cells. Different expression of BPOZ and TIAP/m-survivin between primordial and primary follicles was confirmed by using laser capture microdissection followed by real-time PCR BPOZ and TIAP/m-survivin expressed 4.5 and 3.4 fold higher in primary than primordial follicles, respectively. List of genes obtained from the present study will provide insights for the study of mechanism regulating primordial-primary follicle transition.

• Journal of Internet Computing and Services
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• v.11 no.2
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• pp.61-72
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• 2010

This paper addresses the problem of moving object localization using Ultra-Wide-Band(UWB) range measurement and the method of location accuracy improvement of the indoor moving object. Unlike outdoor environment, it is difficult to track moving object position due to various noises in indoor. UWB is a radio technology that has attention for localization applications recently. UWB's ranging technique offer the cm accuracy. Its capabilities for data transmission, range accurate estimation and material penetration are suitable technology for indoor positioning application. This paper propose a positioning algorithm of an moving object using UWB ranging technique and particle filter. Existing positioning algorithms eliminate estimation errors and bias after location estimation of mobile object. But in this paper, the proposed algorithm is that eliminate predictable UWB range distance error first and then estimate the moving object's position. This paper shows that the proposed positioning algorithm is more accurate than existing location algorithms through experiments. In this study, the position of moving object is estimated after the triangulation and eliminating the bias and the ranging error from estimation range between three fixed known anchors and a mobile object using UWB. Finally, a particle filter is used to improve on accuracy of mobile object positioning. The results of experiment show that the proposed localization scheme is more precise under the indoor.

• Journal of Embryo Transfer
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• v.19 no.3
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• pp.257-264
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• 2004

For safe preservation of Korean Native Pigs (KNP) as an animal genetic resources and a means to maintain the genetic diversity, we performed to investigate the optimal hormone levels for superovulated gilts and establish the cryopresevation methods of embryos. The reults were as follows; 1. The number of ovulated corpus luteums (CL) and follicles were 12.4, 13.6, 30.0 or 23.3 in hCG 500IU and PMSG 500, 750, 1,000IU or hCG 750IU and PMSG 1,000IU respectively. In the case of PMSG 1,000IU와 hCG 500IU, there showed highest number but were no significance among others. The recovery rate of embryos by the ovulated CL were 59.4-79.2％. 2. The morula stage embryos recovered on Day 4 after insemination were significantly higher than Day 5 (P<0.01), but blastocyst stage embryos recovered on Day 5 were sinificantly higher than Day 4 (P<0.05). 3. The survival rate of expanded blastocyst were 23.5％ in conventional freezing with 1.4 M glycerol.

• Korean Journal of Organic Agriculture
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• v.23 no.4
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• pp.975-985
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• 2015

The susceptibility of the twospotted spider mite, Tetranychus urticae and the predatory mite, Phytoseiulus persimilis to extracts of Melia azedarach, Piper nigrum, Syringa velutina and their mixtures was evaluated in laboratory bioassays. Treatments of mixture 1 and 2 were effective against adult females of T. urticae and yielded 73.3 and 70.7% adulticidal activity at 7 days after treatment, respectively. Treatment of mixture 3 revealed 62.7% adulticidal activity. However, M. azedarach, P. nigrum and S. velutina had lower adulticidal activity than the other treatments. Adult females of T. urticae treated with mixture 1 and 2 produced only 11.1-16.7% as many eggs as control females did. All the plant extracts tested were ineffective to against the eggs of T. urticae. Plant extracts tested had little effect on the survival of P. persimilis adult females. Moreover, reproduction of P. persimilis adult females and eclosion of eggs deposited by treated predators were not seriously affected. Treatment of plant extracts tested showed no toxic effect on P. persimilis eggs and produced 100% hatchability. These results suggest that mixture 1 and 2 might be used for the control of T. urticae, and expected to be promising candidates for use in integrated mite management program with P. persimilis.

• The Korean Society of Law and Medicine
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• v.16 no.1
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• pp.83-124
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• 2015

Currently, infertility and artificial insemination have increased. However, there is no detailed legislation about this. In this study, I'll consider the legal status of artificial insemination child. Through the examination of the overseas legislation, we may get the reference of the future legislation. If we consider the increasing of infertility, artificial insemination should be allowed. And due to the well-being of artificial insemination child, such as the United States and France, the artificial insemination child should be regarded the legal child of the infertile couples. When the contradiction between the subject's basic rights has occurred, we need to consider the welfare of the child preferentially. Because the artificial insemination child has no selection of opportunity in birth, unlike adults who select the artificial insemination in their own free will. The development of biotechnology should be for the treatment of human. But creation and improvement of human must be prohibited. In addition, the social investigation of recognition about A.R.T. and general public administrative legal regulation should be made. There is a need for balanced discipline through a multi-disciplinary research. This study may be providing a legal interpretation criteria of other biotechnology researches.

• Parasites, Hosts and Diseases
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• v.22 no.2
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• pp.259-266
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• 1984

Present study was undertaken to elucidate the changes of the ultrastructure of Naegleria fowleri trophozoite in brain tissue of mice and culture medium. Naegleria fowleri, 0359 strain, which used in this study was cultured in axonic liquid medium, CGVS medium. Each mouse was inoculated with amoebas intranasally under secobarbital anesthesia, and sacrificed on 7th day after the infection. Comparative observation of the ultrastructure of the amoebas in axonic culture and experimentally infected mice brain was done with transmission electron microscope. The results are summarized as follows: 1. The amoebas in mouse brain tissue were round in outline, whereas those of amoebas from axonic culture showed irregular appearance. 2. Mitochondria in the amoebas from axonic culture was oval, round and cylindrical shape and darkly stained, whereas those of the amoebas from mouse brain tissue showed dumbbell shape together with above forms. The stain was not unique, but light and/or dark. 3. Rough endoplasmic reticulum of amoebas in brain tissue was tubular, but from culture it was vesicular or tubular in shape. 4. Emity vacuoles were demonstrated in amoebas from culture, while food vacuoles with myelinated structures were abundant in those from tissue, suggesting a strong phagocytic activity. 5. Mouse brain tissue in ected were extensively destroyed, and Polymorphonuclear leukocytes were infiltrated predominantly with inflammatory lesion. Amoebas were observed in the vicinity of the capillary.

• Journal of Food Hygiene and Safety
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• v.17 no.1
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• pp.45-54
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• 2002

Hazard Analysis Critical Control Point System (HACCP) is recognized worldwide as a science-based and systematic approach for food safety. This has been initiated to establish the HACCP system in Korean slaughterhouses by suggesting some of effective implementation ways. An awareness level survey using questionnaire for 124 owners and 363 employees of the slaughterhouses, and 170 government officers responsible for HACCP duties was performed. This study shows that only 34.7% of owners, 21.1% of employees, and 57.1% of government officers have good understanding of HACCP, but 93.5%, 88.9% and 93.6% respectively have the willingness of HACCP implementation. Major benefits in HACCP implementation in the slaughterhouse raised by respondents were improvement of hygiene level of the plant (54.1%), increase of meat sale (17.6%), pride of the production of safe meat for human consumption (9.6%) and increase of meat export (7.5%), while major obstacles were financial difficulties (33.2%), poor levels of the facilities and equipments in the plants (27.3%), poor level of personal hygiene (19.4%), and lack of understanding of HACCP (12.3%). The most urgent measures which respondents raised to implement HACCP system effectively in the slaughterhouses at the moment were the Improvement of levels of facilities and equipments in the plants (36.6%), the establishment of HACCP base through education and training (19%), special benefits to HACCP implementing plant (11.8%), and close relationship between government and the industry (10.3%). The present study shows some of effective ways to implement HACCP in slaughterhouses.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.26 no.3
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• pp.254-264
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• 1990

Using the meteorological data, surface weather map, and oceanographic data for 5 years(1984-1988). I investigated the characteristics of the fog occurrence and the role of the inshore in Pusan about the fog occurrence. And the meteorological data and sea surface temperature(SST), which were observed in July, 1989 in Suyoung Bay, were compared with those in Pusan. The fogs in Pusan concentrate in May, June and July. And at fog occurrence time the principal wind directions are Southwest(SW) winds, which easily supply with water vapor, and a series of Northeast(NE) wind. At the fog days pressure patterns are pattern 7 in spring time (March, April, and May) and pattern 10 and pattern 13 in summer time (June, July, and August). Also the advection fog(sea fog) is closely related with the relationship between warm and cold advection in 850~700mb and cold and warm SST rather than the increase of the instability of atmosphere in 850~500mb. The fogs in Taegu, which is the inland region, mainly occur at dawn in fall time due to the strong night radiation fog. On the other hand in Pusan the coastal region, the fogs occur from late spring time to summer time (May, June, and July). Because there is the abundant supply of the water vapor from the ocean owing to a series of South(S) wind at this time. Then the atmosphere, which has high relative humidity, reaches easily the supersaturation by the radiation cooling. In Suyoung Bay and Pusan the meteorological observation data, SST and fog days are almost similar. And I think that the mechanism of the fog occurrence nearly accords with both regions.

• Journal of fish pathology
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• v.19 no.1
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• pp.7-15
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• 2006

Amyloodinium sp. was found on the gills of mullet (Mugil cephalus) cultured on land. No external symptoms in the diseased fish were found except decoloration of the gills. In fresh preparations of the gills the parasites were opaque round or oval shape with a bright nucleus and 43.5 ㎛ (18.2～72.7, n=20) in size. In preparations added a drop of Lugol solution, they were black with the same shapes in fresh preparations and 43.5 ㎛ (n=20) in size. The parasites were stained black and blue in a droplet of Lugol solution and Diff-Quick III solution, respectively and their sizes were a little larger than in wet preparations. After stained with May-Grunwald Giemsa, the parasites appeared granular eosinophlic in the peripheral cytoplasm and granular strong basophilic in the center. In silver impregnated specimens the peripheral granules were negative and the central ones positive. The granules appeared brown in purplish cytoplasm after staining with Lugol solution. The parasites developed by binary division when they were cultivated in filtered seawater at 20℃. Histopathologically severe epithelial hyperplasia and fusion in the gill filaments resulted in clubbing, especially the proximal region of the filament. Epithelial hyperplasia was also found in the basal regions of the gill filaments and some epithelial cells were occasionally detached from the filaments. Some pear-shaped trophonts of the parasites with rhizoid attached on the gill filaments showing hyperplasia of the epithelial cells and mucous cells.