• 제목/요약/키워드: 김정순

검색결과 268건 처리시간 0.037초

중고등학생의 에이즈 예방교육(豫防敎育)을 통한 에이즈 지식(知識)과 성의식(性意識) 변화(變化)에 관(關)한 연구(硏究) (A Study on the Change of AIDS Knowledge and Sexual Behavior among Middle and High School Students through AIDS Prevention Education)

  • 권관우;이경무;김정순
    • 한국학교보건학회지
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    • 제12권1호
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    • pp.109-129
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    • 1999
  • AIDS is worldwide problem. It has threatens societies and is potentially a big problem among youth. UNAIDS has warned that collective global responses are necessary; with half of the newly infected being children and young people in 1998, educating youth below age 24 is essential. Because of both their unexpected, strong sexual activities and an easjer tendency to change their behavior than adults, UNAIDS emphasizes the importance of prevention education for youth. In Korea, 4 cases of HIV infection have officially been reported among high school students. Considering the potential seriousness of HIV infection among youth, the Korean AntiAIDS Federation (KAAF) began an education programme for Korean youth (middle & high school pupils) as one intervention method in 1993. The purpose of this study is to evaluate the impact of the education programs performed and to develop a better programme. The intervention method used in this study was mainly a lecture in a big or small auditorium or through broadcasting systems using audio-visual teaching aids. The period of this survey was from Nov. 10 to Dec. 26. in 1998. The subjects surveyed were 792 pupils who were sampled from 12 different middle and high schools in Seoul by a two-stage cluster sampling. Self-recording the structured questionnaire was used for data collection. Data were analyzed by using a paired t-test to compare the knowledge difference before and after the interrention method. A chi-square test to compare the consciousness difference between pre-post-education results was used, and an ANOVA was ased to compare the knowledge difference among the selected groups. Major results of this study are summarized as follows: (1) AIDS knowledge significantly changed after AIDS prevention education. (2) The direct lecture in a big or small auditorium is more effective than an indirect lecture through a broadcasting system or through audio-visual teaching aids. (3) Those who had not seen obscene materials (e.g. pornography films, magazines etc.) acguired much more knowledge than others after AIDS prevention education. (4) Those who had no girlfriend/or boyfriend acquired much more knowledge than the others after AIDS prevention education. (5) As to the attitudes toward chastity, more girls than boys and students who had not seen obscene materials rather than those who already had were liable to support the state ment: "all should remain chase" and their consciousness on "being chaste" changed much more than that of others after intervention. (6) As to the individual evaluation of lectures, 23.1% shows "very good", 44.1% "good", 26.9% "normar" '-' thus, the positive evaluation was to 94.2%. The negative response was 5.8%. (7) As to the desire for AIDS education sponsored by KAAF in the future, more than 90.5% showed a positive reaction. (8) As to the educators in future education methods, pupils wanted lecturers from outside their schood. (9) Students from vocational high schools have seen obscene materials much more than other groups and the percentage of those who had a girlfriend/or boyfriend was higher than that of the others as wello. (10) 90% of those who watched obscene materials responded that they experienced these materials while at middle school and 100% before reaching the second grade of high school. (11) The number of boys who have experienced obscene materials is twice as many as that of girls. (12) The percentage of boys who have a girlfriend is much higher than the percentage of girls who have a boyfriend. (13) Among those who have friends of the opposite sex, 11.1% say that they hare had sexual contact and 20.8% hare experienced kissing and caressing.

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산업장 근로자의 산업보건관리에 대한 지식 태도 및 실천에 관한 조사 연구 -부산지역을 중심으로- (A Study of Industrial Workers' Knowledge, Attitude, and Practice of Health Care Services in Busan City)

  • 황보선;신유선;윤석옥;이지현;김정순;김이순;김복용;강영미
    • 지역사회간호학회지
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    • 제4권2호
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    • pp.100-108
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    • 1993
  • The purpose of this study was to identify the workers the Knowledge, Attitude, and Practice of industrial health care services. The study was conducted from Dec 10, 1992 to Jan 20, 1993. The data was collected from 849 workers in 56 companies in Busan City. The data was analyzed by using the mean, S.D>, T-test, ANOVA. The tool for measuring the degree of Knowledge, Attitude and Practice of industrial health care of workers was developed by community health nursing academy and the reliability an of the tool was 0.82810. The results were as follows : 1. The general characteristics of workers : Distribution of workers consisted of laborer(55.0%), office workers(45.0%), in an age range from 25-39(55.0%), male employees were(69.7%), married employees were(62.4%), the educational level with the highest percentage was high school graduates(54.2%). The present work force had a career for 7 years or more with an income of 300-700 thousand won monthly(43.2). 2. The degree of Knowledge Attitude and Practice about industrial health care services of workers : The total score of the Knowledge, Attitude, and Practice was 49.09 points out of a possible 80(mean Score=2.46) 1) The total score of the Knowledge was 15.73 points out of 24. (Mean score=2.62) The following are the Knowledge scores: The necessity examination of occupational disease was(3.34) The knowledge of occupational disease was (3.12) The knowledge of health education was(1.29) 2) b. The total score of the Attitude was 26.01 point out of 44(mean score=2.36) The following are the Attitude scores: The necessity of health education for health examination was (3.14). The importance of health examination was(3.08) The necessity of measurement for working environment was(2.99). The satisfaction of the content in the periodic health examination was low(1.81). 3) The total score of the Practice was 7.35 points out of 12(mean score=2.45) The following are the orders of Practice scores: The participation in health examination was higher than in health education(1.33). 3. The general characteristics and the degree of knowledge, Attitude, and Practice of workers of industrial health care. 1) The level of worker's knowledge was significantly related by working part(t=2.54 P=0.000), marital status(F=4.35 P=0.029), educational level(F=3.91 and P=0.020), monthly income(F=2.98 P=0.029) 2) There were no significant difference between the general characteristics and the attitude of workers. 3) The practice was significantly related with working part(T=2.52 P=0.012), sex(T=2.28 P=0.23), marital status(F=4.25 P=0.012), monthly income (F=2.76 P=0.034) 4) The total score had a significant difference with working part(T=2.39 P=0.017), sex (T=3.84 P=0.000), marital status (F=3.18 P=0.032). Educational level (F=3.20 P=0.033), and monthly income(F=3.05 P=0.022).

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특수건강진단에서 발견된 고혈압 및 간질환 유소견자의 건강관리 실태에 관한 조사 (A Study on the Status of Seeking Intervention among the Workers with Health Problems Identified by the Workers' Periodic Health Examination)

  • 정해관;김정순;문옥륜;임현술
    • Journal of Preventive Medicine and Public Health
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    • 제25권4호
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    • pp.343-356
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    • 1992
  • Authors studied the workers' knowledge about the health problems detected through the previous workers' periodic health examination, content of follow-up management ana actions taken for their health problem detected on previous health examination. From June to September 1992, workers' periodic health examination was peformed on workers employed in 10 companies located in 2 middle-sized Korean cities. A questionnaire survey was done far 150 workers who reported to have $D_2$ result of either hypertension or liver disorder at the previous workers' periodic health examination done in 1991. The results are as follows; 1. Of 160 workers who had $D_2$ result of either hypertension or liver disorder in previous examination one year before, only 85 workers(51.3%, 43 workers with hypertension, 38 workers with live disorder) responded that they have such disorders. The other 65 workers responded to questionnaire were all those with C results. Respondents' knowledge about their diagnoses was relatively precise (95.2% in hypertension group, 94.6% in liver disorder group) but knowledge about classification of diseases was poor. 2. The main efforts to solve the health problem nab self management (20 spells, 55.3%), visiting clinic or hospital(6 spells, 12.8%), use of herb medicine (2 spells, 4.3%) and use of drug store(2 spells, 4.3%) in hypertension group. In liver disorder group, 30 spells (71.4%) relied on self management,6 spells (14.3%) on hospital or clinic and 9 spells (21.4%) had no effort to improve the health problem. Content of self management was low salt diet, quit smoking, regular exercise and quit alcohol drinking in order. Avoidance of salt in diet was high in hypertension group and quitting alcohol drinking was high in liver disorder group. In those with self management, 80.7% of hypertension group and 83.3% of liver disorder group continued previous effort. Those, however, who utilized clinic or hospital, only 16.7% and 50.0% were still visiting hospital or clinic. 3. Fifty seven percent of hypertension group and 64.3% of liver disorder group was presently smoking,8.5% and 11.9% reduced smoking and 21.3% and 14.3% stopped smoking. Forty nine percent of hypertension group and 28.6% of liver disorder group was presently drinking. Reduced alcohol intake was reported in 29.8% and 40.5%, 12.8ole and 23.8% stopped alcohol drinking. Sixty six percent of hypertension group and 73.8% of liver disorder group did no regular exercise, but 12.8% and 11.9% of each group increased their physical exercise far last one year. Forty three percent of hypertension group and 38.l% of liver disorder group was overweight (defined by bodymass index greater or equal than 25). Reduced body Weight was reported in 17.2% and 16.7% of each group. Reduced dietary salt intake was high in hypertension group (51.5%). The study results suggest that follow-up management after workers' periodic health examination is not satisfactory. In order to improve this situation, adequate information on the result of the workers' periodic health examination should be distributed to each worker group with health education and counselling.

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저농도 연폭로에서 혈중 연농도와 자각증상과의 관계 (Relationship of between blood lead level and lead related symptoms in low level lead exposure)

  • 황규윤;안재억;안규동;이병국;김정순
    • Journal of Preventive Medicine and Public Health
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    • 제24권2호
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    • pp.181-194
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    • 1991
  • This study intended to obtain an useful information on the prevalence of subjective symptoms, and to clarify the interrelationships between blood lead and lead related symptoms in low level lead exposure. The 93 male workers exposed to lead and 56 male nonexposed workers were examined for their blood lead(PBB), Zinc-protoporphy(ZPP), hemoglobin(HB) and personnal history, and completed 15 questionnaires related to symptoms of lead absorption : also measured lead concentration in air (PBA) in the workplace. The results obtained were as follows ; 1. The means of blood lead (PBB), blood ZPP and hemoglobin (HB) among workers exposed to lead were $26.1{\pm}8.8{\mu}g/dl,\;28.3{\pm}26.0{\mu}g/dl$ and $16.2{\pm}1.2g/dl$ : whereas those of nonexposed workers were $18.7{\pm}5.1{\mu}g/dl,\;20.6{\pm}8.7{\mu}g/dl$ and $17.3{\pm}1.1g/dl$. The means of above three indicies between two groups showed significant difference statistically (p<0.05). 2. The means of blood lead (PBB), blood ZPP and hemoglobin of workers exposed .to different lead concentration in air were as follows : When it was below $25{\mu}g/m^3$, the indices were $24.7{\pm}79,\;26.1{\pm}26.8{\mu}g/dl\;and\;16.4{\pm}1.1g/dl$ respectively : These indices were $27.1{\pm}8.5,\;23.9{\pm}10.92{\mu}g/dl\;and\;16.2{\pm}1.3g/dl$ when the lead concentration in air was $25{\sim}50{\mu}g/m^3$ : and they were $3.4{\pm}9.3,\;42.3{\pm}31.3{\mu}g/dl\;and\;15.5{\pm}1.2g/dl$ when the concentration of lead was above $50{\mu}g/m^3$. Although there were statistical difference in blood lead and hemoglobin among three different lead concentration in air, there was no statistical difference of blood ZPP among the three groups with different exposure levels (p>0.05). 3. The most frequent by complained symptom was 'Generalized weakness and fatigue', and fewest symptom was 'Intermittent pains in abdomen' 4. Only two symptoms out of fifteen symptoms checked by themselves revealed significant difference between exposed and nonexposed groups. These were 'Intermittent pains of abdomen' and 'Joint pain or arthralgia' (p<0.05), No positive correlation was found between the levels of blood lead and symptom groups categorized as gastrointestinal, neuromuscular and constitutional symptoms, 5. Blood lead (r=0.3995) and ZPP (r=0.2837) showed statistically significant correlation with mean lead concentration in air, whereas correlations were not demonstrated between blood lead and lead related symptoms or blood ZPP and lead related symptoms. 6. Blood lead (PBB) and ZPP showed association (r=0.2466) and the equation PBB=23.75+0.0842 ZPP was derived. 7. On stepwise multiple regression, using blood lead level as a dependent variable and ZPP, hemoglobin (HB), age, work duration (WD) and symptom prevalence as a independent variables, only ZPP significantly contributed a lot to blood lead level. 8. While the ZPP measurement was found to be a good indicator in evaluating health effect of lead absorption in low level lead exposure, lead related symptoms were not sensitive enough to evaluate of lead absorption in low level exposure.

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초음파로 진단된 지방간의 유병율 조사 및 그 유발인자에 대한 연구 (A cross-sectional study on prevalence rate and contributing factors of fatty liver diagnosed by ultrasonography)

  • 안재억;함정오;황규윤;김주자;이병국;남택승;김정순;김헌
    • Journal of Preventive Medicine and Public Health
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    • 제24권2호
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    • pp.195-210
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    • 1991
  • Fatty liver is caused by derangement of fat metabolism and can be reversed by removal of contributing factors. The contributing factors of fatty liver is known to be overweight, chronic alcoholism, diabetes mellitus, malnutrition, and drug abuse such as tetracycline. This study was carried out on 1335 persons who visited 'Soon Chun Hyang Human Dock Center' from March to June 1990. In analysis of the data, prevalence of fatty liver diagnosed by ultrasonogram by age and sex, laboratory finding between fatty liver group and normal group, and odds ratio of known contributing factors, were compared. The results obtained are as following ; 1) The prevalence rate of fatty liver diagnosed by ultrasonogram is 29.6% in male and 11.5% in female. 2) Age groups with high prevalences are $40{\sim}50's$ in male (32.0%) and 50's in female (24.5%). 3) The fatty liver shows significant association with style (p<0.05), whereas not with hepatitis B-virus surface antigen (p>0.05). 4) All laboratory values except alkaline phosphatase and bilirubin are elevated significantly in accordance with the degree of fatty liver (p<0.01). 5) Fatty liver diagnosed by ultrasonogram showed so strong associations with body index, triglycerides and gamma-glutamyl transferase for males, and body index and fasting blood sugar for females that these factors may be used as supplementary data in establishing diagnosis of fatty liver. 6) Odds ratio of contributing factors are as follows ; If the odds ratio of below 29 year of age is 1.0 then that of $30{\sim}39$ is 1.74 (p=0.33), $40{\sim}49$ is 2.47 (p=0.10), $50{\sim}59$ is 2.86 (p=0.0570), over 60 is 1.81 (p=0.34). If the odds ratio of female is 1.0 then that of male is 5.67 (p<0.01). If the odds ratio of body index below zero is 1.0 then that of $0{\sim}9$ is 5.08 (p<0.01), $10{\sim}19$ is 12.37 (p<0.01), $20{\sim}29$ is 29.19 (p<0.01), 30 above is 154.02 (p<0.01). If the odds ratio of below 99 mg/dl FBS is 1.0 then that of $100{\sim}120$ is 106 (p=0.76), over 120 is 1.91 (p=0.02). If the odds ratio of below $29{\mu}/1{\gamma}-GT$ is 1.0 then that of $30{\sim}s59$ is 2.11 (p<0.01), $60{\sim}90$ is 1.87 (p<0.05), 90 above is 1.69 (p=0.15). If the odds ratio of below 149 mg/dl TG is 1.0 then $150{\sim}199$ is 1.49 (p=0.05), $200{\sim}250$ is 1.09 (P=0.77), 250 above is 2.53 (p<0.01). In summary, early diagnosis of fatty liver could be made by ultrasonogram supplemented with body index and nm triglyceride. The fatty liver could be preventive by avoiding contributing factors such as obesity, alcohol intake, high blood sugar appropriately.

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담배 가격인상이 흡연률과 흡연귀속사망에 미칠 영향에 대한 연구 -대도시 일부 사무직 근로자를 대상으로- (A Study on The Effect of The Tobacco Price Raise on The Smoking Rate and Smoking Attributable Death)

  • 강종원;김정순
    • Journal of Preventive Medicine and Public Health
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    • 제30권4호
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    • pp.697-707
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    • 1997
  • 본 연구에서는 대도시 일부 사무직 근로자를 대상으로 흡연에 관한 설문조사를 통해 흡연률, 과거흡연률, 가격변동시 예상흡연률과 그에 따른 흡연의 귀속 사망수 감소 기대수를 조사, 분석하였다. 그 결과는 다음과 같았다. 1. 대도시 지역 남성 사무직 근로자의 흡연률은 59.5%였고 과거흡연률은 28.8%였으며, 과거흡연자를 제외하고 흡연률을 산출할 경우 흡연률은 67.4%였다. 2. 남성흡연자중 담배의 가격과 무관하게 금연할 의사가 있다고 응답한 사람의 분율은 55.3%였고, 담배의 가격이 4배 이상 인상될 경우 금연하겠다고 응답한 사람의 분율은 61.5%로서 반수 이상의 흡연자가 금연하겠다는 의사를 가지고 있는 것으로 나타났다. 3. 남성 흡연자의 흡연시작연령은 반수 이상(62.3%) 만 20세 미만으로, 미성년자 시기에 흡연을 시작하는 경우가 많았다. 4. 담배의 가격인상폭을 크게 할수록 금연 의사가 있는 사람의 비율이 늘어서, 4배이상 가격을 인상할 경우 예상 흡연률이 26.7%였다. 매년 20%씩 담배의 가격이 오를 경우 예상 흡연률은 46.2%였다. 5. 남성을 대상으로 흡연과 관련이 깊은 주요 8개 질환군(폐암, 위암, 췌장암, 후두암, 식도암, 뇌혈관질환, 허헐성심질환, 만성폐쇄성폐질환)의 귀속위험을 추산한 결과 매년 25,863명이 흡연에 기인해서 사망하는 것으로 추산되었다. 이는 20세 이상 총 남성 사인 분류가능 사망수 128,875명의 20.1%이다. 6. 담배의 가격인상시 기대되는 흡연률을 적용하여 계산한 결과 담뱃값을 4배 이상 올렸을 때 금연 실천률을 100%로 가정했을 때 귀속위험은 13,527명으로서 매년 홉연으로 인한 사망의 47.7%인 12,336명의 사망을 줄일 수 있는 것으로 추산되었다. 7. 담배의 가격인상시 금연하도록 동기부여가 되는 사람중에서 실제로 금연하는 사람의 비율을 10%, 25%, 50%로 가정했을 때, 기대 사망수 감소는 가격을 4배 이상 인상했을 때 각각 1,112명, 3,483명, 5,796명 이었다.

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유기농 찰옥수수종자 생산지의 병해충 발생 소장 (Occurrence of Diseases and Insects in Organic Sweet Corn Seed Production Area)

  • 김정순;고병대;곽재균;이명철;김창영;김정곤;심창기
    • 한국유기농업학회지
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    • 제18권1호
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    • pp.93-104
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    • 2010
  • 찰옥수수 유기종자의 안전한 생산을 위해서는 효과적인 병해충 제어가 필수적이므로 2개의 찰옥수수 품종(미백2호, 흑미찰)을 대상으로 2008~2009년에 병해충 발생 양상을 조사한 결과를 요약하면 다음과 같다. 2008년 유기농 찰옥수수 종자 생산지의 우점 비산포자는 Alternaria sp., Cladosporium sp., Pyricularia sp., Collectotrichum sp., Helminthosporium sp., Bipolaris sp. 등이었고, Alternaria sp., Cladosporium sp. 및 Helminthosporium sp.은 전 생육기간에 관찰되었다. 종자 및 유묘에 나타나는 병해충 발생 양상은 종자가 발아하지 않고 썩는 경우와 유묘가 시들어 심하면 고사하는 증상을 보였으며, 거세미나방에 의해 땅가 부위가 잘려진 증상을 관찰할 수 있었다. 종자 및 유묘의 발병주율은 14~16% 미만이었다. 옥수수재배포장에서 가장 큰 피해를 주는 조명나방의 발생밀도 발생밀도는 품종간에는 차이가 없었으며 2008년은 트랩당 3.5~20.5마리였고 2009년은 트랩당 0.5~6마리로 낮게 나타났다. 피해주율은 2008년은 7.5~21%이었으며, 2009년은 1~46%로 수확기까지 지속적으로 그 피해가 증가하였다. 잎마름병의 발병주율은 2008년도에 7~34%였으나 품종간의 차이는 보이지 않았고 2009년도는 6월 18일 처음 발생하였고, 8월 21일 이후 급격히 증가하여(59%) 9월 25일에는 미흑찰(69%)이 미백2호(56%)보다 다소 높게 나타났다. 깜부기병의 발병주율은 2008년도에 5~15%였으며, 2009년에는 7월 17일 처음 발생하여 2008년에 비해 17일 정도 늦게 발생하였으며 발병주율도 8% 미만(9월 25일)으로 2008년에 비해 아주 낮게 나타났다. 깨씨무늬병의 발생주율은 2008년도에 발생 초기에는 평균 11%(미흑찰)이었다가 9월 19일 조사 때는 두 품종 모두 62%로 높게 나타났다. 2009년도 처음 발생 시기는 6월 8일 이후로 2008년에 비해 20일정도 일찍 발생하였으며 9월 25일까지 지속적으로 발병하여 약 86%까지 달하였으며 2008년에 비해 24% 이상 높게 나타났다.

근적외선 분광분석법을 이용한 향미벼의 아밀로스 및 단백질 정량분석 (Quantification of Protein and Amylose Contents by Near Infrared Reflectance Spectroscopy in Aroma Rice)

  • 김정순;송미희;최재을;이희봉;안상낙
    • 한국식품과학회지
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    • 제40권6호
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    • pp.603-610
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    • 2008
  • 본 연구는 향미 및 다면적 재래 벼 유전자원에 대하여 근적외선 분광분석법을 이용하여, 현미 및 벼 상태의 향미자원으로부터 spectrum을 획득 후, 아밀로스함량과 단백질함량분석을 하고자 실시하였다. 75점의 향미 및 다면적 재래 유전자원의 현미로부터 측정한 단백질함량의 범위는 3.8-9.3%였으며, 평균 단백질함량은 7.1%이고 아밀로스함량의 범위는 8.5-27.4%였으며, 평균 아밀로스함량은 20.3%이었다. 79점의 향미 및 다면적 재래 유전자원에 대한 벼 상태 및 현미상태에서의 NIR 원시 spectrum을 나타낸 것으로 1,490 nm 이상의 파장범위에서 큰 차이를 보였다. NIR 원시 spectrum을 MPLS방법에 의해서 벼 상태로부터 얻은 spectrum은 1,4,4,1수 처리 방법, 현미상태로부터 얻은 spectrum은 2,4,4,1 수 처리 방법의 결과가 유의성이 높았다. 벼 상태에 대한 MPLS(1,4,4,1)방법에 의한 $R^2$ 및 SEC 값은 protein은 $R^2$ 값이 0.871, SEC 값이 1.37이었고, amylose는 $R^2$값이 0.815, SEC 값이 0.29이었으며, 현미상태의 경우 MPLS(2,4,4,1)방법에 의한 $R^2$ 및 SEC 값은 protein은 $R^2$값이 0.943, SEC값이 0.90이며 amylose는 $R^2$값이 0.859, SEC 값이 0.37로 높은 유의성을 나타내었다. 습식 분석 데이터와 NIR 예측 data에 대한 차이를 살펴보았더니, 평균 단백질 함량의 차이는 벼 상태는 0.06, 현미상태는 0.12 였고, 평균 아밀로스 함량 차이도 벼 상태는 0.33이었고 현미상태는 0.37로 근소한 차이를 보였다.

잡곡유전자원을 이용한 유기종자생산 포장의 해충발생 소장 (Occurrence of Insect Pest from Organic Seed Producing Field of Minor Grain Germplasms)

  • 김정순;고호철;윤성탁;조양희;김정곤;심창기
    • 한국작물학회지
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    • 제55권1호
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    • pp.58-64
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    • 2010
  • 잡곡유기종자 안정적인 생산을 위해 병해충 관리의 기본이 되는 잡곡의 중요 병해충발생 소장 및 발생시기와 밀도를 조사하고자 본 연구를 실시하였다. 1. 2008년 조, 기장, 수수의 유기잡곡종자 생산 포장에서 조사기간 동안 조명나방의 발생 밀도는 수수자원의 밀도가 가장 높았으며 조자원에서 가장 낮은 발생 밀도를 보였으며, 2009년도 조명나방의 발생 밀도는 수수재배 포장의 밀도가 가장 높았으며 조재배 포장에서 가장 낮은 발생 밀도를 보였다. 2. 2008년 조명나방에 의한 잡곡의 피해주율을 조사하였더니, 지속적으로 증가하여 10월 16일에는 조 (60%), 수수 (47.5%), 기장 (43.8%) 순으로 높게 조사되었다. 2009년에는 6월 8일부터 9월 4일까지 지속적으로 증가하여 9월 4일에는 조 (51.3%), 수수 (42.5%), 기장(32.5%) 순으로 높게 나타났으며 기장자원은 6월 8일부터 6월 26일 사이에 그 피해가 발생하지 않았다. 3. 멸강나방 성충의 발생 시기나 밀도를 조사하였으나, 2008년, 2009년 모두 전혀 유인되지 않았으나 멸강나방 유충에 의한 피해는 지속적으로 관찰되었으며, 각자원별로 피해주율을 살펴보면, 조, 수수, 기장 순으로나타났다. 4. 2008년, 2009년 모두 멸강나방 유충에 의한 피해주율은 조, 수수, 기장 순으로 높게 나타났으며, 2008년 조자원에 대한 피해주율은 11.25~35.0%로 지속적으로 증가하였고, 수수와 기장의 피해주율은 각각 3.8~26.3%, 7.5~22.5%의 변이를 보였다. 2009년 각 자원별로 평균 피해주율은 조 자원이 가장 높게 나타났으며, 조는 2.5~36.3%, 수수는 0~25.0%, 기장은 0~21.3% 순으로 나타났다 5. 잡곡포장에서의 노린재 발생은2008년 풀색노린재, 알락수염노린재, 썩덩나무노린재, 3종류가 발생하였으며, 조와 기장 자원에서 풀색노린재의 발생 밀도가 가장 높았고, 2009년은 4종 (풀색노린재, 알락수염노린재, 썩덩나무노린재, 톱다리노린재)의 노린재가 발생하였고 조와 기장 자원은 풀색노린재와 톱다리노린재가 우점종 이었으며 알락수염노린재의 발생밀도는 낮게 나타났다. 수수 자원은 썩덩나무노린재와 톱다리노린재가 우점종 이었으며 알락수염노린재와 풀색 노린재는 낮은 밀도를 나타내었다.

Gas Chromatography-Mass Spectrometry에 의한 향미 유전자원의 방향성 화합물 비교분석 (Comparison Analysis of Aromatic Compounds in the Aromatic Rice Germplasm by Gas Chromatography and Mass Spectrometry)

  • 김정순;조점래;곽재균;김태산;안상낙;이석영
    • 한국작물학회지
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    • 제54권1호
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    • pp.88-103
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    • 2009
  • 국내 육성향미자원과 도입 향미 유전자원의 2AP 함량을 비교분석하여, 2AP 함량이 높고 농업적 형질이 우수한 자원을 30품종의 향미 유전자원에서 방향성 화합물을 SPME 추출법으로 추출하고 GC-MS로 각 자원이 가지는 방향성 화합물을 비교분석 및 동정하였다. 1. 3종류의 유기용매의 2AP 추출효율은 ethanol > acetonitl > methanol 순으로 확인되었으며, ethnaol의 경우 $30{\sim}90^{\circ}C$의 온도범위에서 3가지 유기용매 중 ethanol이 2AP 추출효과가 가장 뛰어났고, 특히 $90^{\circ}C$에서 30분간 추출하였을 때 2AP 추출함량이 가장 높았고 추출 시간이 경과할수록 2AP의 함량이 감소하였다. 현미의 분쇄정도에 따른 2AP 추출함량은 5초간 분쇄하였을 경우 가장 높았으며 분쇄 시간이 길어질수록 낮아지는 경향이었다. 2. 선발자원의 향에 대한 관능검사는 국내 향미자원 5품종 중 Hyangmibyeo1ho, Arnaghyangchalbyeo가 중간 정도의 향을 띄었고 인디카형 도입자원 19품종 중에서 11품종과 자포니카형 도입자원 6품종 중 2품종이 중간 이상의 향을 나타났다. 3. 30품종의 평균 2AP함량은 국내 육성 향미자원이 도입 향미자원에 비해 낮았으며 도입자원에서는 인디카형이 자포니카형 보다 높은 2AP 함량을 나타내었다. 4. 156종의 방향성 화합물 기능기별로 분류하면 alcohol류 33종, acid류 23종, ketone류 24종, hydrocarbon류 20종, ester류 16종, aldehyde류 16종, ether류 4종, amine류 5종 phenol류 3종, base류 2종, 및 기타 10종으로 분류되었다. 5. 156종의 방향성 화합물 중 생태형별 출현빈도가 50% 이상인 방향성 화합물을 살펴보면, 국내육성 향미자원에서는 2-acetyl-1-pyrroline 외 28종, Japonica type 도입 향미자원에서는 9,12-octadecadienoic acid 외 31종, Indica type 도입 향미자원에서는 naphthalene 외 23종이었다. 6. GC-MS 분석결과 검출된 국내 육성 향미자원의 방향성 화합물의 수는 평균 38개($36{\sim}41$개), 평균 total peak area는 61.1%($51.6{\sim}79.3%$)이었다. 도입 자포니카형 향미자원의 방향성 화합물의 수는 평균 39개($26{\sim}45$개), 평균 total peak area는 63.2%($40.9{\sim}81.9%$)이었다. 도입 인디카형 향미자원의 방향성 화합물의 수는 평균 39.6개($26{\sim}53$개), 평균 total peak area는 72.8%($33.8{\sim}98.8%$)이었다. 7. 30 품종으로부터 검출된 156종의 방향성 화합물의 출현 유무(0/1)를 기본 matrix로 하여, UPGMA/Neighbor-join tree 분석을 통해 분류한 결과, 7개의 그룹(Group $I{\sim}VII$)으로 나눠어졌다. 각 그룹의 66% 이상의 자원에서 나타난 향(aroma/odor)성분을 살펴보면, alcohol, aldehyde, acid, ether류 등의 휘발성 성분들의 비율이 품종별로 높았다. 따라서 본 실험의 결과는 대량의 향미 유전자원의 방향성 화합물에 대한 GC/GC-MS분석결과로, 향후 우수한 향미 품종 육성에 필요한 자료로 활용될 수 있다고 사료된다.