• Korean Journal of Veterinary Research
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• v.50 no.3
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• pp.221-229
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• 2010

Encephalomyocarditis virus (EMCV) belongs to the genus Cardiovirus within the family Picornaviridae. EMCV has been recognized either as a cause of mortality in young pigs, due to acute myocarditis, or of reproductive failure in sows. An EMCV K3 strain was isolated from the heart and brain in a mummified and aborted swine fetus in 1989. For the molecular characterization of the poly(C)-tract of EMCV Korean isolates, K3 strain, viral RNA was extracted and digested with RNase T1, and analyzed the length of the poly(C)-tract by polyacrylamide gel electrophoresis. The poly(C) regions also were amplified by RT-PCR and sequenced. The present study shows that K3 strain of EMCV had a short polymorphic poly(C) tracts (5 to 30 C's) with sequences consisting of $C_9$, $C_{10}$, $C_{13}$, $C_{14}$, $C_{16}$, $C_{20}$, $CUC_{11}$, $C_8UCUC_3UC_{10}$, $C_9UCUC_3UC_{10}$, $C_{10}UCUC_3UC_{10}$, etc. These polymorphism of poly(C)-tracts of EMCV K3 strain implies the historical information of in vivo and/or in vitro passage.

• The Journal of Pediatrics of Korean Medicine
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• v.20 no.3
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• pp.75-85
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• 2006

Objectives : The somatization is various physical symptoms that have no pathologic manifestation. It is very common to Korean children and adolescents as well as adults, because cultural background and immature verbal expression. DSM-Ⅳ(Diagnostic and Statistical Manual of Mental Disorders Fourth Edition) presented diagnostic criteria for Somatization disorder by grouping some symptoms out of somatization. This report is on a case of somatization disorder patient. The patient is 13-year-old female. She has been suffered from various physical symptoms those are coincided with diagnostic criteria for Somatization disorder by DSM-Ⅳ. Methods : The authors treated her by acupuncture and 3 kinds of herb medicine-Bunsimgium, Hyungbangdojuksan, Yangguksanwhatang. Results : Bunsimgium was showed a little improvement but could not solved chief complaint. Hyungbangdojuksan was not given a help. After her taking Yangguksanwhatang, most symptoms were subsided. Conclusions : A patient with the somatization disorder was improved by herbal medication and acupuncture. But the Somatization disorder repeats to take a good turn and a bad turn and continues for long time, so we need to follow up her condition.

• The Journal of Pediatrics of Korean Medicine
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• v.20 no.3
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• pp.97-104
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• 2006

Objectives : There were few reports on Erysipelas, from Secondary Skin Infection after Herpes Zoster. We treated Erysipelas, from Secondary Skin Infection after Herpes Zoster with Oriental medical approach, and got a significant results. So We are reporting this case. Methods : Herb medication, acupuncture treatment were applied for treating patient`s chief symptoms (erythema, heat sensation, swelling, pain, itching and general weakness) Results : The patient was 5-year-old boy who had the symptoms caused by Erysipelas, which was caused by secondary skin infection after herpes zoster. He had erythema, heat sensation, swelling, pain, itching and general weakness. We judged him as Soyangin(少陽人), and prescribed Hyeongbangsaback-san. After he took Hyeongbangsaback-san, the symptom and general condition were improved. In this report, we want to explain the healing process and the result of the treatment of Erysipelas. Conclusions : More study about oriental treatment on Erysipelas, from Secondary Skin Infection after Herpes Zoster is needed.

• Journal of agricultural medicine and community health
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• v.23 no.1
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• pp.79-89
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• 1998

This study was to evaluate the performance status and associated factors with the health promoting activities. Two hundred three old aged over 65 years old sampled by quota sampling among 660 old aged in a rural area, Dae-Ma Myun, Young-Kwang Gun, Chollanam Do. The questionnaire survey was performed from June 1st to August 31st in 1997. The major findings were as follows ; 1. The performance rate of health promoting activities were higher in female, dwellers at community health practitioner post area, and old aged believes in a religion and high educated old aged. 2. A multiple regression analysis showed that physical health promoting activity was related to psychiatric wellbeing status, the disability of daily living, residental area and family numbers(P<0.05). 3. The disability of daily living and psychiatric wellbeing status were related to mental health promoting activities by a multiple regression analysis(P<0.05). In conclusion, psychiatric wellbeing status, the disability of daily living, the disability of daily living and residental area were associated the performance of health promoting activities for old aged in a rural area.

• The Journal of Korean Society for School & Community Health Education
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• v.16 no.1
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• pp.51-63
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• 2015

목적: 사하라 이남 아프리카 지역 중저소득국가 모성보건지표의 더딘 개선율은 MDG 5(모자보건향상) 미달성의 주요 원인 중 하나로 꼽힌다. 따라서 본 연구는 우간다 동부 카프초르와 구의 모성사망과 밀접한 산후건강관리(PNC, Postnatal care)서비스 이용결정요인을 파악하는데 있다. 이를 통해 지역건강관리자들에게 PNC 서비스 이용 개선을 위한 정책 수립 및 방안 마련에 기초자료를 제공하고, 궁극적으로는 MDG 5 지표 개선에 일조하고자 한다. 방법: 표본 집단은 카프초르와 구의 15세~49세 여성들 중 최근 1년 내에 출산을 경험한 자들을 대상으로 편의추출 되었다. 조사기간은 2014년 7월부터 10월까지였으며, 구조화된 설문에 총 171명이 응답하였고, 19명의 주요 정보제공자와의 심층면담도 실시하였다. 응답자의 사회인구학적 특성 및 PNC 이용행태를 알아보기 위해 빈도분석을 실시하였으며, 각 독립변수가 PNC 이용에 어떤 영향을 미치는지 파악하기 위해 로지스틱 회귀분석을 실시하였다. 결과: 응답자의 55%만이 의료시설의 PNC 서비스를 받은 것으로 나타났다. 로지스틱 회귀분석을 통해서는 응답자의 연령과 사회적 네트워크, 인지된 건강상태, 산전관리서비스 이용이 PNC 서비스 이용에 긍정적인 영향을 미치는 것으로 나타났으며 의료시설과의 거리, 가족의 규모는 부정적인 영향을 미치는 것으로 나타났다. 결론: PNC 서비스 이용개선을 위해서는 먼저 여성의 사회적 자본 확충 및 개선을 위한 모성보건교육인 소프트 인프라 지원이 지자체 차원에서 실시되어야 할 것이며, 서비스 이용을 가능케 하고 접근성을 높이는 응급후송체계 구축과 같은 물리적 인프라 지원도 도입되어야할 것이다. 또한 가족계획 서비스를 제공하는 등 모성보건관리에 대한 지자체의 민감성을 높이는 것도 필요하겠다.

• Health Policy and Management
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• v.7 no.2
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• pp.46-64
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• 1997

Background & Objectives : Korea is face with the social need for health care technology assessment so that it is urgently needed to found principles and methodology in technology assessment in health care. As a groundwork for health care technology assessment, we tried to prioritize medical technology for assessment. Among medical technologies, procedure is somewhat difficult to assess, compared to drug or equipment. In this study, we aimed at the prioritisation of medical procedure to be assessed, in terms of efficay, safety, and adequacy. Method : For the standardized classification of medical procedure, ICD-9-CM(International Classification of Diseases 9th edition - Clinical Modification) was used. Among the list the procedures coming under otorhinolaringjology and thoracic surgery were selected by three family physicians. The list of procedure was mailed to the board certified surgeons of both disciplines, with the question asking about the necessity for assessment in terms of efficay, safety, and adequacy. Replied questionnaires were analyzed in each procedure. Results : Of 560 otorhinolaryngologist and 480 thoracic surgeon, 114 surgeons replied. Of otorhinolaryngological procedure, incision, excision, and destruction of inner ear : fenestration of inner ear : stapedectomy and its revision were the most urgent technology to assess in the aspect of safety. For adequacy, operations on Eustachian tube: fenestration of inner ear: incision, excision, and destruction of inner ear were highly ranked in necessity, and for efficary, operations on Eustachian tube; external maxillary antrotomy; fenestration of inner ear. Thoracic surgeons replied thoracic procedures, lung transplantation; heart transplantation; implantation of heart assist system [pump] are most important for evaluation in terms of safety; and heart transplantation; Lung transplantation; Implantation of heart assist system [pump] in terms of adequacy, and surgical collapse of lung [Artificia니 pnemothorax or pnuexoperitoeum]; lung transplantation; periarterial sympathectomy in terms of efficacy. As a whole, surgeons regard safety evaluation is more urgent than adequacy or efficary. In addition, otorhinolaryngological surgeons regard evaluation of their procedures more urgent than thoracic surgeons regard theirs. Conclusion : By the questionnaire to board certified physicians, we get some preliminary data for prioritisation of technologies to assess. Through the questionnaire like this, much information would be gathered for technology assessment, especially for medical procedure, if not enough. In the near future, well structured expert opinion gathering research, such as modified Delphi or nominal group technique, should be done succeedingly.

• Journal of the korean academy of Pediatric Dentistry
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• v.48 no.3
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• pp.302-312
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• 2021

The purpose of this study was to develop prototype of virtual reality(VR) simulation with malocclusion models and evaluate its applicability. Task abilities, task completion time, and a satisfaction survey were compared between dentist trainees and dental students. Participants were instructed to observe virtual malocclusion models and then performed three tasks to diagnose the type of malocclusion, determine clinical findings and develop treatment plans. Their satisfaction with the simulation experience were evaluated using a questionnaire containing five questions. Task abilities of trainees related to clinical features and treatment plans were significantly higher than that of students(p < 0.01). In both groups, the task completion time for the second case was significantly reduced compared to that for the first case(p < 0.01). The satisfaction survey showed high scores and positive responses for this simulation in both groups. If the prototype of VR simulation is continuously advanced, it will be applicable for orthodontic education in pediatric dentistry.

• Journal of Apiculture
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• v.34 no.1
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• pp.27-37
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• 2019

Rapid detection of Tropilaelaps, an external parasite of honeybees that lead to malformation of honeybee or colony collapse disorder, is becoming important. But it is very difficult to find with the naked eye of Tropilaelaps. In this study, we have developed a method to detect the specific gene of Tropilaelaps from the hive debris and to know the number of Tropilaelaps in the hive through Tropilaelaps-specific quantitative detection. Tropilaelaps-specific gene amplified in DNA extracted from hive debris by consecutive PCR (1st detection, 2nd nested PCR). It could detect 101 molecules level of Tropilaelaps-specific gene and confirm the amplification of the Tropilaelaps-specific gene. It was possible to accurately quantify the number of Tropilaelaps from the hive debris sample, which is difficult to discriminate the presence of Tropilaelaps visually, through Tropilaelaps-specific detection. Under the microscope, Tropilaelaps was collected and quantitative detection of Tropilaelaps-specific genes was performed. It was possible to quantify the number of Tropilaelaps present in the hive through the molecules of the quantified Tropilaelaps-specific genes. We suggest that hive debris can represent as a micro-environment to hive and show that it can be a simpler and more accurate sample than using a parasitic host honeybee. We expect that hive debris should facilitate the monitoring of Tropilaelaps in hive.

• Journal of Apiculture
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• v.34 no.1
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• pp.39-46
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• 2019

BQCV multi-point PCR was developed as a rapid multiplex detection method for BQCV, one of the viral pathogens of honeybees. It could detect BQCV specific genes qualitative as well as quantitative detection based on ultra-rapid PCR. Three primer pairs (RNA dependent RNA polymerase, capsid protein, 3C like protease) were specifically designed for accurate the detection and were optimized for minimizing the detection time and increasing the sensitivity. Our advanced diagnostic system have the accuracy by lowering the concern about the variation in the BQCV detection site. In addition, it should be an opportunity to identify mutations that are mixed with other viruses.

• Journal of Korean society of Dental Hygiene
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• v.22 no.1
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• pp.63-72
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• 2022

Objectives: This study was conducted to prepare basic data to propose the necessity and utilization of oral welfare products in the welfare services of the long-term care insurance system, focusing on facility workers working in elderly facilities. Methods: The analysis was conducted on 144 workers working at some local elderly facilities. The questionnaire was constructed by classifying the use of oral welfare tools into 6 questions and the necessity and demand for oral welfare devices into 13 questions. Frequency analysis and technical analysis were performed for data analysis, and one-way ANOVA was performed for differences in the necessity and demand for oral welfare equipment. The statistical significance level was p<0.05. Results: As a result of examining the awareness of the necessity and demand for oral welfare equipment among workers in elderly facilities, the awareness of the necessity of including oral welfare equipment in the items of welfare equipment in the current long-term care insurance system was high at 4.15 points. As a result of analyzing the correlation between awareness of care products and the need and demand for oral welfare equipment, it was confirmed that there was a statistically significant positive correlation (p<0.01). Conclusions: In the long-term care insurance system for the elderly, oral welfare products need to be considered for welfare equipment services. The provision of oral welfare products within the long-term care insurance system for the elderly can provide opportunities and services to select various self-care tools. In addition, it is expected that it will be possible to promote changes in the long-term care insurance system for the elderly and to improve the system in a variety of positive ways.