Journal of Apiculture (한국양봉학회지)

The Apicultural Society of Korea (한국양봉학회)
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Quantitative Detection of Tropilaelaps in Hive by Specific Gene Detection from Hive Debris

봉변에서 특이 유전자 검출법에 의한 봉군 내 꿀벌가시응애류 (Tropilaelaps)의 정량적 검출

  • Kim, Byounghee (Department of Life Science, College of Fusion Science, Kyonggi University) ;
  • Kim, Somin (Department of Life Science, College of Fusion Science, Kyonggi University) ;
  • Kim, Moonjung (Department of Life Science, College of Fusion Science, Kyonggi University) ;
  • Kim, Jungmin (Department of Life Science, College of Fusion Science, Kyonggi University) ;
  • Truong, A Tai (Department of Life Science, College of Fusion Science, Kyonggi University) ;
  • Kim, Seonmi (Department of Life Science, College of Fusion Science, Kyonggi University) ;
  • Yoon, Byoungsu (Department of Life Science, College of Fusion Science, Kyonggi University)
  • 김병희 (경기대학교 융합과학대학 바이오융합학부 생명과학과) ;
  • 김소민 (경기대학교 융합과학대학 바이오융합학부 생명과학과) ;
  • 김문정 (경기대학교 융합과학대학 바이오융합학부 생명과학과) ;
  • 김정민 (경기대학교 융합과학대학 바이오융합학부 생명과학과) ;
  • ;
  • 김선미 (경기대학교 융합과학대학 바이오융합학부 생명과학과) ;
  • 윤병수 (경기대학교 융합과학대학 바이오융합학부 생명과학과)
  • Received : 2019.03.11
  • Accepted : 2019.04.23
  • Published : 2019.04.30
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Abstract

Rapid detection of Tropilaelaps, an external parasite of honeybees that lead to malformation of honeybee or colony collapse disorder, is becoming important. But it is very difficult to find with the naked eye of Tropilaelaps. In this study, we have developed a method to detect the specific gene of Tropilaelaps from the hive debris and to know the number of Tropilaelaps in the hive through Tropilaelaps-specific quantitative detection. Tropilaelaps-specific gene amplified in DNA extracted from hive debris by consecutive PCR (1st detection, 2nd nested PCR). It could detect 101 molecules level of Tropilaelaps-specific gene and confirm the amplification of the Tropilaelaps-specific gene. It was possible to accurately quantify the number of Tropilaelaps from the hive debris sample, which is difficult to discriminate the presence of Tropilaelaps visually, through Tropilaelaps-specific detection. Under the microscope, Tropilaelaps was collected and quantitative detection of Tropilaelaps-specific genes was performed. It was possible to quantify the number of Tropilaelaps present in the hive through the molecules of the quantified Tropilaelaps-specific genes. We suggest that hive debris can represent as a micro-environment to hive and show that it can be a simpler and more accurate sample than using a parasitic host honeybee. We expect that hive debris should facilitate the monitoring of Tropilaelaps in hive.

Keywords

References

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