Journal of Apiculture (한국양봉학회지)

The Apicultural Society of Korea (한국양봉학회)
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Development of Diagnostic System to Black Queen Cell Virus(BQCV) Using Multi-point Detection

Multi-point PCR법을 이용한 Black Queen Cell Virus (BQCV) 검출법 개발

  • Kim, Somin (Department of Life Science, College of Fusion Science, Kyonggi University) ;
  • Kim, Byounghee (Department of Life Science, College of Fusion Science, Kyonggi University) ;
  • Kim, Moonjung (Department of Life Science, College of Fusion Science, Kyonggi University) ;
  • Kim, Jungmin (Department of Life Science, College of Fusion Science, Kyonggi University) ;
  • Truong, A Tai (Department of Life Science, College of Fusion Science, Kyonggi University) ;
  • Kim, Seonmi (Department of Life Science, College of Fusion Science, Kyonggi University) ;
  • Yoon, Byoungsu (Department of Life Science, College of Fusion Science, Kyonggi University)
  • 김소민 (경기대학교 융합과학대학 바이오융합학부 생명과학과) ;
  • 김병희 (경기대학교 융합과학대학 바이오융합학부 생명과학과) ;
  • 김문정 (경기대학교 융합과학대학 바이오융합학부 생명과학과) ;
  • 김정민 (경기대학교 융합과학대학 바이오융합학부 생명과학과) ;
  • ;
  • 김선미 (경기대학교 융합과학대학 바이오융합학부 생명과학과) ;
  • 윤병수 (경기대학교 융합과학대학 바이오융합학부 생명과학과)
  • Received : 2019.03.11
  • Accepted : 2019.04.23
  • Published : 2019.04.30
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Abstract

BQCV multi-point PCR was developed as a rapid multiplex detection method for BQCV, one of the viral pathogens of honeybees. It could detect BQCV specific genes qualitative as well as quantitative detection based on ultra-rapid PCR. Three primer pairs (RNA dependent RNA polymerase, capsid protein, 3C like protease) were specifically designed for accurate the detection and were optimized for minimizing the detection time and increasing the sensitivity. Our advanced diagnostic system have the accuracy by lowering the concern about the variation in the BQCV detection site. In addition, it should be an opportunity to identify mutations that are mixed with other viruses.

Keywords

References

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