• Horticultural Science & Technology
• /
• v.34 no.4
• /
• pp.537-548
• /
• 2016

The objective of this study was to evaluate the antimicrobial activities of 9 kinds of medicinal plants against crown gall in grapevine. The medicinal plants extracted with several solvent systems were screened for in vitro antibacterial activity by the disc diffusion method. The ethanol and ethyl acetate extracts from magic lily flowers, tachys roots, asian plantain flowers and seeds, sweet wormwood leaves, stems and flowers, immature bitter melon fruits, cockscomb flowers, and peach tree resin showed in vitro antimicrobial activities against Rhizobium vitis with growth inhibition zones ranging from 10 to 27 mm in diameter. The minimum inhibitory concentration values of extracts against R.vitis ranged from 10,000 in Asian plantain flower and 50,000 fold diluted extracts in sweet wormwood flowers, stems, leaves, cockscomb leaves and immature bitter melon fruits. The active fractions of ethyl acetate and ethanol extracts from the medicinal plants were partially separated through silica gel column chromatography and thin layer chromatography (TLC). The active fractions were separated at Rf 0.36, 0.69, 0.75, 0.84, and 0.94 in sweet wormwood extracts, Rf 0.96 and 0.99 in cockscomb flower extracts, Rf 0.92 and 0.97 in cockscomb leaf extracts, and Rf 0.85 in immature bitter melon fruit extracts in TLC analysis developed with hexane:ethyl acetate (20:80, v/v) and methanol:chloroform (20:80, v/v). Among extracts from plants with in vitro antimicrobial activities, sweet wormwood, cockscomb leaves, and immature bitter melon fruits showed in vivo antimicrobial activities with inhibition activity of 100, 67, and 83.3%, respectively, in 'Kyoho' grapevine inoculated with R. vitis compared with the untreated control. These findings indicate that extracts of medicinal plants could be used as sustainable candidates to control crown gall disease caused by R. vitis in grapevines.

• Applied Chemistry for Engineering
• /
• v.26 no.1
• /
• pp.104-110
• /
• 2015

It is known that polymer concretes are 8~10 times more expensive than ordinary Portland cement concretes; therefore, in the production of polymer concrete products, it is very important to reduce the amount of polymer binders used because this occupies the most of the production cost of polymer concretes. In order to develop a technology for the reduction of polymer binders, smooth and spherical aggregates were prepared by the atomizing technology using the oxidation process steel slag (electric arc furnace slag, EAFS) and the reduction process steel slag (ladle furnace slag, LFS) generated by steel industries. A reduction in the amount of polymer binders used was expected because of an improvement in the workability of polymer concretes as a result of the ball-bearing effect and maximum filling effect in case the polymer concrete was prepared using the smooth and spherical atomized steel slag instead of the calcium carbonate (filler) and river sand (fine aggregate) that were generally used in polymer concretes. To investigate physical properties of the polymer concrete, specimens of the polymer concrete were prepared with various proportions of polymer binder and replacement ratios of the atomized reduction process steel slag. The results showed that the compressive strengths of the specimens increased gradually along with the higher replacement ratios of the atomized steel slag, but the flexural strength showed a different maximum strength depending on the addition ratio of polymer binders. In the hot water resistance test, the compressive strength, flexural strength, bulk density, and average pore diameter decreased; but the total pore volume and porosity increased. It was found that the polymer concrete developed in this study was able to have a 19% reduction in the amount of polymer binders compared with that of the conventional product because of the remarkable improvement in the workability of polymer concretes using the spherical atomized oxidation steel slag and atomized reduction steel slag instead of the calcium carbonate and river sand.

• Journal of Bio-Environment Control
• /
• v.22 no.1
• /
• pp.27-33
• /
• 2013

In order to provide basic data for the development of a controlled environment cultivation system and standardization of the structures, structural status and improvement methods were investigated for the fruit tree greenhouses of grape, pear, and peach. The greenhouses for citrus and grape cultivation are increasing while pear and persimmon greenhouses are gradually decreasing due to the advance of storage facilities. In the future, greenhouse cultivation will expand for the fruit trees which are more effective in cultivation under rain shelter and are low in storage capability. Fruit tree greenhouses were mostly complying with standards of farm supply type models except for a pear greenhouse and a large single-span peach greenhouse. It showed that there was no greenhouse specialized in each species of fruit tree. Frame members of the fruit tree greenhouses were mostly complying with standards of the farm supply type model or the disaster tolerance type model published by MIFAFF and RDA. In most cases, the concrete foundations were used. The pear greenhouse built with the column of larger cross section than the disaster tolerance type. The pear greenhouse had also a special type of foundation with the steel plate welded at the bottom of columns and buried in the ground. As the results of the structural safety analysis of the fruit tree greenhouses, the grape greenhouses in Gimcheon and Cheonan and the peach greenhouses in Namwon and Cheonan appeared to be vulnerable for snow load whereas the peach greenhouse in Namwon was not safe enough to withstand wind load. The peach greenhouse converted from a vegetable growing facility turned out to be unsafe for both snow and wind loads. Considering the shape, height and planting space of fruit tree, the appropriate size of greenhouses was suggested that the grape greenhouse be 7.0~8.0 m wide and 2.5~2.8 m high for eaves, while 6.0~7.0 m wide and 3.0~3.3 m of eaves height for the pear and peach greenhouses.

• Journal of The Korean Society of Grassland and Forage Science
• /
• v.22 no.4
• /
• pp.265-272
• /
• 2002

Forage breeding laboratory of National Livestock Research Institute, R.D.A. has made interspecific hybrids of Lolium multiflorum $\times$ L. pratensis and intergeneric hybrids of Lolium $\times$ Festuca since 1984, and has collected ecotypes of Italian ryegrass since 1991. Growth characteristics of these hybrids and ecotypes were researched, and then these clone lines were named. Among these clone lines, the several clones that have polen fertility, high cold-tolerance, and similar heading time were used for making synthetics, Naehan 6, 7, 8, 9, with polycrossing method in 1997. Field experiments were carried out to compare the mophological and agronomical characteristics and forage productivity and quality of the synthetics with those of Italian ryegrass varieties, Barmultra and Hwasan 101. in Suwon and Yonchun from 1999 to 2000. Heading time of the synthetics were 22th to 24th May that belong to late-mature types to be similar to that of Barmultra and Hwasan 101 in Suwon. The synthetics were 101 to 106 c3n in plant length, medium or thick in thickness of stem, dark peen in leaf color, broad and long in flag leaf, strong in lodging resistance, and excellent in regrowth. Winter survivals of the synthetics were no different from that of Barmultra or Hwasan 101 in Suwon, but better than that of Barmultra or Hwasan 101 in Yonchun where was -10 to -12$^{\circ}C$ of minimum average air temperature in January or February. Dry matter(DM) yields of the synthetics were similar to DM 8,238kg per ha of Barmultra in Suwon, but in Yonchun, were more 7 to 13% than DM 7,291kg per ha of Barmultra. Forage qualities, IVDMD, ADF, NDF and TDN of the synthetics were lower than those of Hwasan 101, but higher than those of Barmultra.

• Journal of The Korean Society of Grassland and Forage Science
• /
• v.18 no.3
• /
• pp.171-178
• /
• 1998

This experiment was conducted to study on forage utility of Diplachne fusca L. which live in reclaimed saline land of midwest region of Korea. The primary experiment was conducted to know on germination characteristics of Diplachne fusca L. according to storage condition after seed harvesting, light, temperature, flooding depth, salinity and soil covering. The results obtained are summarized as follows; 1. The germination percentage, average days to germination, germination rate and coefficient of germination were not different in existence of light, combination with existence of light and storage condition, but those were higher different in storage condition. 2. The germination characteristics were more higher on the condition of alternating temperature than constant temperature, and it was the highest on the condition of alternating temperature with $35/25^{\circ}C$ and constant temperature with $35^{\circ}C$. 3. There were apparently significant in germination percentage, average days to germination, germination rate and coefficient of germination according to flooding depth. The germination characteristics of D. fusca L. were the highest on the wndition of flooding depth with Ocm and did not germinate on the condition of flooding depth more than 3cm. 4. There were apparently significant in germination percentage, average days to germination, germination rate and coefficient of germination according to salinity. Germination characteristics were the highest on the condition of salinity with 0.2%. There was more than 30% of germination percentage on the condition of salinity with 1% SO Diplachne fuaca L. was suitable to cultivate in reclaimed saline land. 5. The germination percentage, average days to germination, germination rate and coefficient of germination were significantly difference between soil covering. So Germination characteristics were the highest on the condition of soil covering with 0.2cm. But they scarcely germinated on the condition of more than 1cm of the soil covering.

• Journal of The Korean Society of Grassland and Forage Science
• /
• v.29 no.3
• /
• pp.197-210
• /
• 2009

To improve the forage quality and reduce nitrogen input, trial was conducted on the effect of seeding method, combination, and nitrogen fertilizer with WCB (whole crop barley) and leguminous plant. Present experiment was carried out by split-split design having seeding methods for main plot, combinations for sub-plot, and nitrogen fertilizers for sub-sub plot with three replications. When WCB and leguminous plant were mixed-sown, WCB showed earlier heading and maturing than those of inter-sown, and the more nitrogen delayed growth stage a little. Occurrence of BaYMV (Barley Yellow Mosaic Virus), a serious disease caused by soil fungi and decrease barley yield, was deterred by mixed-seeding as compared to inter-sown barley a little. Inter-sown WCB increased the number of spike per $m^2$ as compared to mixed-seeding showing more spikes with nitrogen increase. WCB produced much fresh and dry matter yield at mixed-seeding than inter-seeding, and had advantage with hairy vetch (HV). Increased nitrogen showed much forage yield, however, half application of it is considerable for environmental-friendly farming. Electric conductivity (EC) decreased in inter-cropping or mixed-sowing soil with WCB and leguminous crop after harvest. But, organic matter (OM) content of soil after harvesting was vice versa. Acid detergent fiber (ADF) and Neutral detergent fiber (NDF) of WCB plant were higher at mixed-seeding than those of inter-sown ones. It showed increased tendency with time progress.

• Korean Journal of Soil Science and Fertilizer
• /
• v.34 no.6
• /
• pp.387-393
• /
• 2001

To use as a fundamental data for the sustainable agriculture, which is nowadays a major trend to keep the productivity and conserve the environment, 487 paddy soil samples were collected from 21 regions of the Gyeongnam Province and analyzed the chemical characteristics and microbial population of the soil. The microbial population densities were bacteria $298{\times}10^5$($4{\sim}3000{\times}10^5$ range), fungi $63{\times}10^3$($2{\sim}441{\times}10^3$ range), actinomycetes $19{\times}10^5$($0.2{\sim}1250{\times}10^5$ range), Bacillus sp. $53{\times}10^4$($4{\sim}890{\times}10^4$ range) and Pseudomonas sp. $198{\times}10^4CFU\;g^{-1}$($4{\sim}1724{\times}10^4CFU\;g^{-1}$ range), respectively. The microbial populations of the soil were in general higher in southern area than in the northern area of the Gyeongnam Province. The average ratio of bacteria/fungi population was 473. As soil clay content increased, the populations of aerobic bacteria, actinomycetes and Pseudomonas sp. were remarkably decreased. The ratio of aerobic bacteria and fungi was 1554 in sandy loam and clay loam 1144, while Bacillus sp./fungi ratio was 11 in clay loam and 10 in loam. On the topographical differences, aerobic bacteria and Bacillus sp./fungi ratio were the higher in coastal plains than any other areas. The microbial population densities from different soil types were generally lower in ill-drained paddy field than those of other paddy field. The content of $P_2O_5$, K, Ca, $NO_3-N$ and EC in soil were positively correlated to the population densities of aerobic bacteria, actinomycetes, fungi, Bacillus sp. and Pseudomonas sp.. The soil organic matter and Mg content were also positively correlated to the population densities of aerobic bacteria, actinomycetes, fungi and Bacillus sp.

• Transactions of the Korean Society for Noise and Vibration Engineering
• /
• v.25 no.1
• /
• pp.48-57
• /
• 2015

In a sodium-cooled fast reactor, which is a Generation-IV reactor, refueling is conducted by rotating, but not opening, the reactor head to prevent a reaction between the sodium, water and air. Therefore, an inspection technique that checks for the presence of any obstacles between the reactor core and the upper internal structure, which could disturb the rotation of the reactor head, is essential prior to the refueling of a sodium-cooled fast reactor. To this end, an ultrasound-based inspection technique should be employed because the opacity of the sodium prevents conventional optical inspection techniques from being applied to the monitoring of obstacles. In this study, a ranging inspection technique using a plate-type ultrasonic waveguide sensor was developed to monitor the presence of any obstacles between the reactor core and the upper internal structure in the opaque sodium. Because the waveguide sensor installs an ultrasonic transducer in a relatively cold region and transmits the ultrasonic waves into the hot radioactive liquid sodium through a long waveguide, it offers better reliability and is less susceptible to thermal or radiation damage. A 10 m horizontal beam waveguide sensor capable of radiating an ultrasonic wave horizontally was developed, and beam profile measurements and basic experiments were carried out to investigate the characteristics of the developed sensor. The beam width and propagation distance of the ultrasonic wave radiated from the sensor were assessed based on the experimental results. Finally, a feasibility test using cylindrical targets (corresponding to the shape of possible obstacles) was also conducted to evaluate the applicability of the developed ranging inspection technique to actual applications.

• Parasites, Hosts and Diseases
• /
• v.21 no.2
• /
• pp.241-245
• /
• 1983

PraziquantEI ($Distocide^{\circledR}$), the KcrEan product, was tEstEd for its safety and Efficacy in treatmEnt of Clonorchis sinensiJ infccticn during the period from April to SeptembEr, 1983 in Korea. A total of 55 egg positive cases were selected and treated with the regimen of 25 mg/kg t.i.d. for 1 day (total 75 mg/kg). The follow-up stool examination was done in 47 cases by cellophane thir;k smear and Stoll's egg counting techniques. The 8 uncured cases were treated again with the same regimen. The laboratory tests for blood picture and liver function were done in 27 cases and compared before and after the treatment. The results obtainEd are as follows: 1. After single course treatment, the cure and egg reduction rates were 83.0 and 99.1% respectively. With the second treatment, excellent results of 100% in both rates were obtained. 2. Several kinds of side effects such as dizziness, headache, etc. were complained by 29 cases (61.7 %), however, those were so mild and transient that no special treatment was necessary. 3. No significant change in laboratory findings was recognizable before and after the treatment. From the above results, it is concluded that $Distocide^{\circledR}$ is as effective and safe as $Biltricide^{\circledR}$ and highly recommendable in treatment of C. sinensis infection.

• Korean Journal of Soil Science and Fertilizer
• /
• v.37 no.3
• /
• pp.177-183
• /
• 2004

The discharge of waste nutrient solution from greenhouse to natural ecosystem leads to the accumulation of excess nutrients that results in contamination or eutrophication. There is a need to recycle the waste nutrient solution in order to prevent the environmental hazards. The amount and kind of nutrients in waste nutrient solution might be enough to grow photosynthetic microorganisms. Hence in the present study, we examined the growth and mass cultivation of cyanobacteria in the waste nutrient solution with an objective of removing N and P and concomitantly, its mass cultivation. Four photosynthetic filamentous cyanobacteria (Anabaena HA101, HA701 and Nostoc HN601, HN701) isolated from composts and soils of the Chungnam province were used as culture strains. Among the isolates, Nostoc HN601 performed faster growth rate and higher N and P uptake in the BG-II ($NO_3{^-}$) medium when compared to those of other cyanobacterial strains. Finally, the selected isolate was tested under optimum conditions (airflow at the rate of $1L\;min^{-1}$. in 15 L reactor, initial pH 8) in waste nutrient solution from tomato hydroponic in green house condition. Results showed to remove 100% phosphate from the waste nutrient solution in the tomato hydroponics recorded over a period of 7 days. The growth rate of Nostoc HN601 was $16mg\;Chl-a\;L^{-1}$ in the waste nutrient solution from tomato hydroponics with optimum condition, whereas growth rate of Nostoc HN601 was only $9.8mg\;Chl-a\;L^{-1}$ in BG-11 media. Nitrogen fixing capacity of Nostoc HN601 was $20.9nmol\;C_2H_4\;mg^{-1}\;Chl-a\;h^{-1}$ in N-free BG-11. The total nitrogen and total phosphate concentration of Nostoc HN601 were 63.3 mg N gram dry weight $(GDW)^{-1}$ and $19.1mg\;P\;GDW^{-1}$ respectively. Collectively, cyanobacterial mass production using waste nutrient solution under green house condition might be suitable for recycling and cleaning of waste nutrient solution from hydroponic culture system. Biomass of cyanobacteria, cultivated in waste nutrient solution, could be used as biofertilizer.