• Title/Summary/Keyword: 기술기회

Search Result 1,661, Processing Time 0.03 seconds

Comparison of Polymerase Chain Reaction, Real-time Polymerase Chain Reaction, and Loop-Mediated Isothermal Amplification for the Detection of Cronobacter sakazakii in Milk Powder (분유에 오염된 Cronobacter sakazakii 검출을 위한 중합효소연쇄반응, 실시간중합효소연쇄반응, 등온검출법의 비교)

  • Kim, Young-Joo;Seo, Sheungwoo;Wang, Xiaoyu;Seo, Dong Joo;Lee, Min Hwa;Son, Na Ry;Lee, Bog-Hieu;Choi, Changsun
    • Food Science of Animal Resources
    • /
    • v.33 no.5
    • /
    • pp.610-616
    • /
    • 2013
  • Loop-mediated isothermal amplification (LAMP) is an emerging detection technology for the amplification of DNA under isothermal conditions. The aim of this study was to develop a rapid and reliable LAMP technique for the detection of Cronobacter sakazakii in milk powder. In order to enhance the sensitivity and specificity, LAMP primers targeting outer membrane protein A (ompA) gene of C. sakazakii were designed using Explorer V4 software. Thirty seven C. sakazakii strains and 13 pathogenic microorganisms were used for comparative detection of C. sakazakii using polymerase chain reaction (PCR), real-time PCR, and LAMP. LAMP developed in this study could specifically detect C. sakazakii strains without cross-reactivity with other foodborne pathogens. LAMP products amplified from ompA gene of C. sakazakii were digested with with HhaI and NruI enzyme. The specificity of LAMP was confirmed by restriction fragment length polymorphism (RFLP) analysis. LAMP could detect C. sakazakii within 1 h without bacterial culture and its detection limit was as low as 1 CFU/mL C. sakazakii in milk. In the comparison of the sensitivity, LAMP showed 10,000- and 100-times higher detection limit than PCR or real-time PCR, respectively. Therefore, this study can conclude that LAMP is a rapid and reliable detection technique for C. sakazakii contaminated in powdered milk.

An Integrated Approach for Korea's e-Navigation Development Project to Playing the Global Lead in Maritime Safety Logistics of IMO's Next Generation (한국의 이네비게이션개발사업이 IMO차세대 해사안전보안물류상 세계 주역을 맡기 위한 통합적 접근)

  • Gim, Jin-Goo
    • Proceedings of the Korean Institute of Navigation and Port Research Conference
    • /
    • 2016.05a
    • /
    • pp.58-58
    • /
    • 2016
  • This study aims to enhance Korea's competitiveness by taking the lead in the international standardization and relevant global market. This is to be achieved by performing the role of First Mover through the resolute investment from Fast Follower that is servile to the decision of the organization in the e-Navigation strategy that has been promoted by the IMO. The study methodology is an integrated approach. It is established by modelling LSCM G2B2C2 Platform in maritime safety/security logistics and applied to the enhancement of maritime safety/security in global LSCM. This paper externally contributes to the reduction of world maritime accidents and exalt the national prestige; internally, the enhancement of the national competitiveness through the improvement of shipping port logistics and others. These eventually contribute to Korea's economic development that is combined by both theory and practice and of practical use of the integrated approach.

  • PDF

A Study on the Establishment of Industrial Security Education Programs in Korea (한국 산업보안교육 프로그램의 정립에 관한 연구)

  • Choi, Sun-Tae;Yu, Hyeong-Chang
    • Korean Security Journal
    • /
    • no.25
    • /
    • pp.185-208
    • /
    • 2010
  • This study is to suggest the current security education programs and improvement of industrial security curriculums in Korea. We live in a world of insecurity; the world is changing at an ever accelerating pace. Life, society, economics, international relations, and security risk are becoming more and more complex. The nature of work, travel, recreation, and communication is radically changing. We live in a world where, seemingly with each passing year, the past is less and less's guide to the future. Security is involved in on one way or another in virtually every decision we make and every activity we undertake. The global environment has never been more volatile, and societal expectations for industrial security and increasing if anything. The complexities of globalization, public expectation, regulatory requirements, transnational issues, jurisdictional risks, crime, terrorism, advances in information technology, cyber attacks, and pandemics have created a security risk environment that has never been more challenging. We had to educate industrial security professional to cope with new security risk. But, how relevant is a college education to the security professional? A college degree will not guarantee a job or advancement opportunities. But, with a college and professional training, a person has improved chances for obtaining a favored position. Commonly, Security education and experience are top considerations to find a job so far, also training is important. Today, Security is good source to gain competitive advantage in global business. The future of security education is prospect when one considers the growth evident in the field. Modern people are very security-conscious today, so now we had to set up close relevant industrial security programs to cope with new security risk being offered in colleges or several security professional educational courses.

  • PDF

Multi-view Video Coding using View Interpolation (영상 보간을 이용한 다시점 비디오 부호화 방법)

  • Lee, Cheon;Oh, Kwan-Jung;Ho, Yo-Sung
    • Journal of Broadcast Engineering
    • /
    • v.12 no.2
    • /
    • pp.128-136
    • /
    • 2007
  • Since the multi-view video is a set of video sequences captured by multiple array cameras for the same three-dimensional scene, it can provide multiple viewpoint images using geometrical manipulation and intermediate view generation. Although multi-view video allows us to experience more realistic feeling with a wide range of images, the amount of data to be processed increases in proportion to the number of cameras. Therefore, we need to develop efficient coding methods. One of the possible approaches to multi-view video coding is to generate an intermediate image using view interpolation method and to use the interpolated image as an additional reference frame. The previous view interpolation method for multi-view video coding employs fixed size block matching over the pre-determined disparity search range. However, if the disparity search range is not proper, disparity error may occur. In this paper, we propose an efficient view interpolation method using initial disparity estimation, variable block-based estimation, and pixel-level estimation using adjusted search ranges. In addition, we propose a multi-view video coding method based on H.264/AVC to exploit the intermediate image. Intermediate images have been improved about $1{\sim}4dB$ using the proposed method compared to the previous view interpolation method, and the coding efficiency have been improved about 0.5 dB compared to the reference model.

A Study on The Effects of Business Plan upon Firm Performance (사업계획이 경영성과에 미치는 영향에 관한 연구: 구성요소 및 기업가유형, 발전단계 측면에서)

  • Koh, In-Kon
    • Asia-Pacific Journal of Business Venturing and Entrepreneurship
    • /
    • v.6 no.4
    • /
    • pp.111-135
    • /
    • 2011
  • While previous studies and publications all assert a strong correlation between company's business plan and performance, very few have actually conducted practical analyses to support that. This study takes a practical approach in its analysis of Korean small and mid-sized enterprises(SME) with the view to finding an answer to the question. In addition, with the considerations of entrepreneur type and company's development stage, I analyzed the differences of business plan components' effects on performances. I selected business plan's components, which have been suggested only in theory and in concept, through the literature review and preliminary examination. Corporate performances were the recent improvements of ROS, ROA, market share and the number of employees to measure how greatly each is impacted by the components of a business plan. Results show that business plan components have influenced upon the number of employees. The business plan components discriminated superior company group and inferior company group properly. Especially, finance & related system and advertising & distribution factors showed statistically significant classification forecasting power. Technical/Craftsman evaluated the effects of producing & sales and profit & quality factors high and General/Opportunistic evaluated the effects of finance & related system, advertising & distribution, corporate mission factors high. The effect of corporate mission was highest among company development stages. Finance & related system and advertising & distribution factors showed the statistically significant difference in entrepreneur type and company development stages.

  • PDF

A Meta-Analysis of Relationship between Perceived Value, Risk and Behavioral Intention on E-Commerce (전자상거래 연구에서 인지된 가치, 위험 및 행위의도 간의 관계에 대한 메타분석)

  • Nam, Soo-tai;Jin, Chan-yong
    • Asia-Pacific Journal of Business Venturing and Entrepreneurship
    • /
    • v.11 no.4
    • /
    • pp.179-189
    • /
    • 2016
  • Recently, the convergence of knowledge based society and information telecommunication technologies has a rapid impact on politics, economics and various fields. Meta-analysis is a statistical integration method that delivers an opportunity to overview the entire result of integrating and analyzing many quantitative research results. Meta-analysis, can see the direction and size of the relationship between variables using the concept of the effect size. The factor determining behavioral intention of consumer in e-commerce can say that critically dependent variable. In a predictive factor determining behavioral intention is typical that perceived value and perceived risk. We conducted a meta-analysis and review of between perceived value, perceived risk and behavioral intention on e-commerce researches. This study focused a total of 33 research papers that established causal relationships in between perceived value, risk and behavioral intention on e-commerce published in Korea academic journals during 2000 and 2016. The result of the meta-analysis might be summarized that the effect size in the path from the perceived value to the behavioral intention with the effect size (r = .526), listed an explanatory power of 28%. In addition, it showed that the effect size in the path from perceived risk to the behavioral intention with the effect size (r = -.220), listed a negative explanatory power of 5%. Based on these findings, several theoretical and practical implications were suggested and discussed with the difference from previous researches.

  • PDF

A Study on the chair design -from the Victorian era to the present- (의자 디자인에 관한 고찰 -빅토리아시대로부터 현재까지-)

  • 정의철
    • Archives of design research
    • /
    • v.15 no.4
    • /
    • pp.177-188
    • /
    • 2002
  • A thousands of chairs have designed from the Victorian era to the present. Thonet's Nol4 chair in 1859, Wright's high-back chair in 1904, Ritveld's red/blue chair in 1917, Breuer's Wassily chair in 1925, Aalto's Paimio chair in 1932, Eames's DAR chair in 1948, Piero Gatti's Sacco chair in 1969, Starck's Von Volgelsang chair in 1984, Stumpf's Aeron Chair in 1992, Little's 'Coat of arms' chair in 1994 - the list of architects who have seized the opportunity to express their theories in the design of a chair is seemingly endless. Architects such as Machintosh(1868-1928), Wright(1807-1959), Aalto(1898-1976) included chairs within their artistic schemes for interiors and buildings. But as the manufacture of chairs moved away from the domain of the craftsman towards that of the industrial process, architects were also ideally positioned, with their background knowledge of engineering, to pioneer innovative chair design within the constraints of modern manufacturing technology. Beyond matters of function and structure, the fundamental worth of chairs, past or present, lies in their communication of attitudes, ideas and values. The persuasiveness of a chair depend on the clarity of its rhetoric. Chair has become an ideal medium for designers to make their visual statements and construct their individual manifestos. In chair design there is a ping-pong game played out between absurd and useful design, and this game is one way in which the design profession explores itself.

  • PDF

A Case study on Reorganization of Specialized High School Through Workforce demand of Electric-Electronic-Communication Industry in Gyeonggi Province and SWOT Analysis (지역 기반 산업의 인력 수요와 SWOT 분석을 통한 특성화 고등학교 개편에 관한 사례연구)

  • Yi, Sang-Bong
    • 대한공업교육학회지
    • /
    • v.36 no.2
    • /
    • pp.65-84
    • /
    • 2011
  • This Study was to conducted in order to suggest future direction for reorganization of Suwon ${\bigcirc}{\bigcirc}$ Women's High School as a vocational high school. The purpose of this Study was to provide suggestions for the Suwon ${\bigcirc}{\bigcirc}$ women's high school through workforce demand of electric-electronic-communication industry in Gyeonggi Province and SWOT Analysis. The findings of the study were as follows: First, the high school need to work out a educational strategy geared to workforce demand of region based electric-eElectronic-communication industry in Gyeonggi Province for students' employment. Second, the following two approaches are recommended for curriculum development to meet region based electric-eElectronic-communication industry in Gyeonggi Province for students' employment. (1) School curriculum development that is geared to a enterprise for students' employment. Demand of the enterprise is reflected when curriculum is developed under cooperation between school and enterprise. Specific knowledge, attitude and skills is emphasized to students according to demand of the enterprise. (2) school curriculum development that is geared to industry for students' employment. Demand of the electric-electronic-communication industry in Gyeonggi Province is reflected when curriculum is developed under cooperation between school and the electric-electronic-communication industry. Broader knowledge, attitude and skills is emphasized to students for the industry.

QR code as Interactive Marketing Communication Media : Focused on Technology Acceptance Model (인터랙티브 마케팅커뮤니케이션 매체로써 QR코드 이용에 관한 연구 : 기술수용모델을 중심으로)

  • Shin, Dong-Hee;Chang, Woo Sung
    • The Journal of the Korea Contents Association
    • /
    • v.13 no.3
    • /
    • pp.76-86
    • /
    • 2013
  • This study examined users' acceptance process with the QR code as an interactive marketing communications tool. We collected total 200 survey data and 181 questionnaires among them were used for the analysis during for five days, from 9 to 13 in May 2011. The first research question of this study examined the relationship among subjective norms, perceived usefulness, perceived ease of use, attitudes, and intention to use through the structural equation modeling analysis. The result of the first research showed that subjective norms about the use of QR code affect intention to use via perceived usefulness, and also directly influence on it. The perceived usefulness has the biggest impact on the intention to use. The research question 2 was to look at the condition effect with the presence of experience. As the analysis result of the second research question, the relationships between subjective norms and perceived usefulness are not different whether or not groups experience QR code, and both groups appeared to have had significant impact. Whereas the relationship between subjective norms and intention to use showed a significant influence on the population that has the experience with the QR code. In order to encourage use of the QR code, therefore, we will need to highly impress on users subjective norms which are ambient pressure on the use of QR code and positively offer marketing strategy which gives users the opportunities to get experience with the QR code.

The analysis of Complex Type and the Facility Status of Community Mixed-support Facility (공동체복합지원시설의 시설현황 및 복합유형 분석)

  • Kim, Sumi;Kim, Suk-Young
    • The Journal of the Korea Contents Association
    • /
    • v.13 no.6
    • /
    • pp.507-517
    • /
    • 2013
  • Welfare desire has been diversified according to the increase of economic level and rapid social change. The desire for comprehensive service on culture, medical, mental and information level has appeared. Accordingly, it is necessary to establish an integrated community mixed-support facility, a space of accepting administration, welfare, leisure, culture and informatization request in a small local community in the future. The community mixed-support facility is a meeting place of local resident for information change, various basic service and ultimately for activation of community in order to contribute to regeneration and activation of existing urban core. Thus, this study figured out general status of community mixed-support facility and physical mixed type and analyzed spatial characteristics. Current community mixed-support facility has insufficient program and space to participate in by local residents and to accommodate mixed function. The future planned community mixed-support facility must secure multiple opportunities for various types of life by returning time and cost to user through the introduction of creative mixed concept. While reflecting unique local characteristics, and considering diversity of users, it needs to make mixed facility to cope with social changes. This study is limited in that the study reached a conclusion by survey and analysis only. However, it is expected that the result can be used as basic research data for the planning of community mixed-support facility to cope with living conditions of local residents or realistic requirements.