Comparison of Polymerase Chain Reaction, Real-time Polymerase Chain Reaction, and Loop-Mediated Isothermal Amplification for the Detection of Cronobacter sakazakii in Milk Powder
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Kim, Young-Joo
(Department of Nutrition Education, Chung-Ang University)
Seo, Sheungwoo (School of Food Science and Technology, Department of Food and Nutrition, Chung-Ang University) Wang, Xiaoyu (School of Food Science and Technology, Department of Food and Nutrition, Chung-Ang University) Seo, Dong Joo (School of Food Science and Technology, Department of Food and Nutrition, Chung-Ang University) Lee, Min Hwa (School of Food Science and Technology, Department of Food and Nutrition, Chung-Ang University) Son, Na Ry (School of Food Science and Technology, Department of Food and Nutrition, Chung-Ang University) Lee, Bog-Hieu (School of Food Science and Technology, Department of Food and Nutrition, Chung-Ang University) Choi, Changsun (School of Food Science and Technology, Department of Food and Nutrition, Chung-Ang University) |
1 | Alex, P. and Georg, E. S. (1998) Structure of the outer membrane protein A transmembrane domain. Nat. Struct. Biol. 5, 1013-1017. DOI ScienceOn |
2 | Arad, I., Baras, M., Gofin, R., Bar-Oz, B., and Peleg, O. (2001) Does parity affect the neonatal outcome of very low birth weight infants. Eur. J. Obstet. Gynecol. Reprod. Biol. 94, 283-288. DOI ScienceOn |
3 | Derzelle, S., Dilasser, F., Maladen, V., Soudrie, N., Leclercq, A., Lombard, B., and Lafarge, V. (2007) Comparison of three chromogenic media and evaluation of two molecular-based identification systems for the detection of Enterobacter sakazakii from environmental samples from infant formulae factories. J. Food Prot. 70, 1678-1684. |
4 | Enosawa, M., Kageyama, S., Sawai, K., Watanabe, K., Notomi, T., Onoe, S., Mori, Y., and Yokomizo, Y. (2003) Use of loop-mediated isothermal amplification of the IS900 sequence for rapid detection of cultured Mycobacterium avium subsp. paratuberculosis. J. Clin. Microbiol. 41, 4359-4365. DOI ScienceOn |
5 | Farmer, J. J., Asbury, M. A., Hickman, F. W., and Brenner, D. J. (1980) Enterobacter sakazakii: A new species of "Enterobacteriaceae" isolated from clinical specimens. Int. J. Syst. Bacteriol. 30, 569-584. DOI |
6 | Friedemann, M. (2007) Enterobacter sakazakii in food and beverages (other than infant formula and milk powder). Int. J. Food Microbiol. 116, 1-10. DOI ScienceOn |
7 | Guillaume-Gentil, O., Sonnard, V., Kandhai, M. C., Marugg, J. D., and Joosten, H. (2005) A Simple and rapid cultural method for detection of Enterobacter sakazakii in environmental samples. J. Food Prot. 68, 64-69. |
8 | Hara-Kudo, Y., Nemoto, J., Ohtsuka, K., Segawa, Y., Takatori, K., Kojima, T., and Ikedo, M. (2007) Sensitive and rapid detection of vero toxin-producing Escherichia coli using loop-mediated isothermal amplification. J. Med. Microbiol. 56, 398-406. DOI ScienceOn |
9 | Hara-Kudo, Y., Yoshino, M., Kojima, T., and Ikedo, M. (2005) Loop-mediated isothermal amplification for the rapid detection of Salmonella. FEMS Microbiol. Lett. 253, 155-161. DOI ScienceOn |
10 | Hu, L., Zhang, W., Zhang, X., Yuan, Y., Zhang, Y., Zhang, H., Ma, X., and Su, X. (2009) Development of a loop-mediated isothermal amplification assay for rapid detection of Enterobacter sakazakii in powdered infant formula. Wei Sheng Wu Xue Bao. 49, 378-382. |
11 | Iversen, C. and Forsythe, S. J. (2003) Risk profile of Enterobacter sakazakii, an emergent pathogen associated with infant milk formula. Trends Food Sci. Tech. 14, 443-454. DOI ScienceOn |
12 | Mohan Nair, M. K. and Venkitanarayanan, K. (2007) Role of bacterial OmpA and host cytoskeleton in the invasion of human intestinal epithelial cells by Enterobacter sakazakii. Pediatr. Res. 62, 664-669. DOI ScienceOn |
13 | Iversen, C., Mullane, N., McCardell, B., Tall, B. D., and Lehner, A. (2008) Cronobacter gen. nov., a new genus to accommodate the biogroups of Enterobacter sakazakii, and proposal of Cronobacter sakazakii gen. nov., comb. nov., Cronobacter malonaticus sp. nov., Cronobacter turicensis sp. nov., Cronobacter muytjensii sp. nov., Cronobacter dublinensis sp. nov., Cronobacter genomospecies 1, and of three subspecies, Cronobacter dublinensis subsp. dublinensis subsp. nov., Cronobacter dublinensis subsp. lausannensis subsp. nov. and Cronobacter dublinensis subsp. lactaridi subsp. nov. Int. J. Syst. Evol. Microbiol. 58, 1442-1447. DOI ScienceOn |
14 | Liu, C., Zheng, W., Zhang, H., Hou, Y., and Liu, Y. (2009) Sensitive and rapid detection of Enterobacter sakazakii in infant formula by loop-mediated isothermal amplification method. J. Food Safety 29, 83-94. DOI ScienceOn |
15 | Liu, Y., Cai, X., Zhang, X., Gao, Q., Yang, X., Zheng, Z., Luo, M., and Huang, X. (2006) Real time PCR using TaqMan and SYBR Green for detection of Enterobacter sakazakii in infant formula. J. Microbiol. Meth. 65, 21-31. DOI ScienceOn |
16 | Mori, Y., Nagamine, K., Tomita, N., and Notomi, T. (2001) Detection of loop-mediated isothermal amplification reaction by turbidity derived from magnesium pyrophosphate formation. Biochem. Biophys. Res. Commun. 289, 150-154. DOI ScienceOn |
17 | Nagamine, K., Hase, T., and Notomi, T. (2002) Accelerated reaction by loop-mediated isothermal amplification using loop primers. Mol. Cell. Probe. 16, 223-229. DOI ScienceOn |
18 | Notomi, T., Okayama, H., Masubuchi, H., Yonekawa, T., Watanabe, K., Amino, N., and Hase, T. (2000) Loop-mediated isothermal amplification of DNA. Nucleic Acids Res. 28, E63. DOI |
19 | Saito, R., Misawa, Y., Moriya, K., Koike, K., Ubukata, K., and Okamura, N. (2005) Development and evaluation of a loopmediated isothermal amplification assay for rapid detection of Mycoplasma pneumoniae. J. Med. Microbiol. 54, 1037-1041. DOI ScienceOn |
20 | Orlandi, P. A., and Lampel, K. L. (2000) Extraction-free, filter-based template preparation for rapid and sensitive PCR detection of pathogenic parasitic protozoa. J. Clin. Microbiol. 38, 2271-2277. |
21 | Singh, S. P., Williams, Y. U., Miller, S., and Nikaido, H. (2003) The C-terminal domain of Salmonella enterica serovar typhimurium ompA is an immunodominant antigen in mice but appears to be only partially exposed on the bacterial cell surface. Infect. Immun. 71, 3937-3946. DOI |
22 | Yamazaki, W., Taguchi, M., Ishibashi, M., Kitazato, M., Nukina, M., Misawa, N., and Inoue, K. (2008) Development and evaluation of a loop-mediated isothermal amplification assay for rapid and simple detection of Campylobacter jejuni and Campylobacter coli. J. Med. Microbiol. 57, 444-451. DOI ScienceOn |
23 | Ye, Y., Wu, Q., Yao, L., Dong, X., Wu, K., and Zhang, J. (2009) A comparison of polymerase chain reaction and international organization for standardization methods for determination of Enterobacter sakazakii contamination of infant formulas from Chinese mainland markets. Foodborne Pathog. Dis. 6, 1229-1234. DOI ScienceOn |
24 | Yoshida, A., Nagashima, S., Ansai, T., Tachibana, M., Kato, H., Watari, H., Notomi, T., and Takehara, T. (2005) Loop-mediated isothermal amplification method for rapid detection of the periodontopathic bacteria Porphyromonas gingivalis, Tannerella forsythia, and Treponema denticola. J. Clin. Microbiol. 43, 2418-2424. DOI ScienceOn |
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