• The Journal of the Petrological Society of Korea
• /
• v.23 no.4
• /
• pp.405-418
• /
• 2014

Ontake Volcano, Japan, began to erupt without any precursors on September 27, 2014, at 11:52 AM, and it caused many losses of life. Although Japan's preparation manual and prevention for volcanic eruptions and volcanic hazards has been well established, it could not prevent damage due to the sudden eruption of the volcano. Soon after the eruption, however, Japan Meteorological Agency (JMA) led many organizations and institutions, including JMA's Volcanic Eruption Prediction Liaison Council, Meteorological Research Institute (MRI) and National Agriculture and Food Research Organization and they understood the eruption situation quickly and shared the information based on their close cooperation and contact systems. Through these efforts, JMA published the unified result to the public, informing the public of the situation around the volcano and about the eruption and of how the residents and climbers around the volcano should react to the volcanic hazards caused by the eruption. The Korean Government can learn how to respond to a future eruption of a volcano, such as Mt. Baekdu which has the potential to erupt in the foreseeable future.

• Journal of Plant Biotechnology
• /
• v.29 no.1
• /
• pp.25-29
• /
• 2002

The study was carried out to establish an efficient protocol for shoot organogenesis and plant regeneration from stem explant cultures of Lycopersicon esculentum cv. Micro-Tom. The regenerated shoots obtained from stem explant cultures on solid MS medium containing the different concentrations of BAP. The highest number of shoots (5.3) per explant and shoot growth (0.7 cm) was obtained on MS medium containing 4.0 mg/L BA. The additions of AgNO$_3$ and putrescine substantially improved the shoot regeneration frequency, at the optimal concentration of 7 mg/L and 50 mg/L respectively. The regenerated shoots (about 1 cm) were normal and could be easily rooted with 0.1 mg/L IBA treatment. The rooted plants were hardened and transferred to vermiculite with a 92% survival rate where they grew normally.

• KSBB Journal
• /
• v.7 no.2
• /
• pp.85-91
• /
• 1992

The role of NAA(1-Naphthaleneacetic acid) and BA(6-Benzyladenine) in the adventitious shoot regeneratlon from Populus leaf segments and changes in the pattern of RNA and protein synthesis were investigated. The adventitious shoot regeneration octured at the basal cut end of Populus leaf segments. This process was effected by many factors, including wounding culture conditions, light and plant growth regulators etc. The highest adventitious shoot regeneration frequency was obtained at $0.01mg/\ell$ NAA with $0.2mg/\ell$ BA. In this condition adventitious shoot starved to regenerate on the 13th day of oullure. The most optimal hormone composition for RNA and protein synthesis was $0.01mg/\ell$ NAA with $0.2mg/\ell$ BA. The content of RNA and protein was greater at the proximal part. In the course of adventitious shoot regeneration, the proteins associated with polar-regeneration appeared at the proximal part of populus leaf segment.

• Korean Journal of Plant Tissue Culture
• /
• v.24 no.6
• /
• pp.329-334
• /
• 1997

Adventitious organs (shoots and roots) from mature leaves of Populus deltoides (Eastern cottonwood) clones were induced on WPM (Woody Plant Medium) containing fructose as a carborn source and different combinations of either BA (Benzyl aminopurin) or TDZ (Thidiazuron) with NAA (Naphthaleneacetic acid). The morphologies of adventitious shoots were different depending on the kinds of growth regulators and the length of shoots induced on the medium containing BA were generally longer than that of TDZ. BA 0.5 mg/L and TDZ 0.01 mg/L combined with either 0.05 or 0.1 mg/L NAA showed the highest values in the frequency of regeneration (63% to 100%) the number of shoots per explant and in the organogenic sites per explant among tested three clones (A, B and C). Although there was a minor clonal difference in the development of adventitious shoots from leaf segments, their responses to growth regulators were very similar.

• The Microorganisms and Industry
• /
• v.14 no.3
• /
• pp.12-14
• /
• 1988

빛에 의한 생물체의 분화는 작용파장별로 분류 할 때 고등식물에서 흔히 나타나는 붉은 빛에 의한 분화와 곰팡이류에서 볼 수 있는 푸른빛에 의한 것 두 종류가 있다. 곰팡이는 Chlorophyll이나 Phytochrome이 없어 붉은ㅅ개 파장을 흡수하는 phytochrome에 의한 분화는 없으나 푸른색은 곰팡이의 생활사에 중요한 요인이 된다. 예를 들면 Phycomyces와 Trichoderma의 photophorogenesis, Cyathus와 Coprinus의 fruiting body형성, Poycomyces와 Neurospora의 photocarotenogenesis, Phycomyces와 Pilobolus의 phototropism등 곰팡이의 metabolism과 development가 푸른 빛에 의하여 영향을 받는다. 한편 곰팡이의 포자및 포자생성기관은 녹색, 갈색, 흑색 등 phenoloxidase의 산물인 melanin 색소를 가지는 경우가 많으며 그 예로는 Podospora, Schizophyllum, Aspergillus 등과 같이 reproduction 할 때 phenoloxidase가 직접적 혹은 간접적으로 연관되어 있다. 버섯형성균류의 하나인 Coprinus congregatus는 버섯형성과정에서 빛을 필요로 하며 이때 phenoloxidase가 어떠한 역할을 하는지 실험하였다.

• Journal of Life Science
• /
• v.8 no.5
• /
• pp.479-485
• /
• 1998

The induction of nephric duct from Xenopus presumptive ectoderm(animal cap) was studied and the high-dose ef-fect of IGF-1 was investigated. Activin A induce various organs from cultured animal cap explants and the effects are time and dose-dependent. On the induction of nephric duct, the combined-dose of activin A and retinoic acid was very efficient method in reference study. In present study, we used IGF-1 as well as activin A as a combined growth factor. The concentration ranges of growth factors were activin A l00ng/ml an IGF-1 0-500ng/m1. Explants were cultured in combined solution for 3days to the normal embryo arrives at st. 43. In general, when the explant was cultured in high concentration(l00ng/ml) of activin A, it was destroyed, however, nephric duct and other tis-sues were differentiated by adding IGF-1. In addition, eye induction by adding IGF-1 500ng/ml to activin A 1- 100 ng/ml solution was studied. The low concentration of activin A(1ng/ml) have blood-like cell inducing effect and the explant was balloon-shaped, however, the high dose combination with IGF-1 extended the range of eye inductive concentration of activin A.

• Korean Journal of Plant Tissue Culture
• /
• v.26 no.3
• /
• pp.197-203
• /
• 1999

The albino plants of tobacco (Nicotiana tobacum L. cv. BY-4) were isolated from seed populations that were induced by heavy-ion ($^{14}N$) beam irradiation to proembryo and the in vitro growth and differentiation have been characterized. The in vitro cultured albino plants showed significant reduction of chlorophyll content and possessed larger number of stomata on both upper and lower epidermis than that of wild-type plants. Stem growth of the mutants remained dwarfed, however, the internode recovered its normal length after GA$_3$ treatment (10.0mg/L) on the MS medium containing sucrose under continuous light. When explants of leaf blades of albino plants were cultured, multiple shoots formed directly on MS medium containing 1.0mg/L of BAP or kinetin and a large number of calli were induced on the MS medium containing 1.0mg/L NAA or 1.0 mg/L 2,4-D. The albino calli regenerated multiple albino plantlets in the MS medium containing 0.1mg/L NAA + 1.0 mg/L BAP. No significant differences between the wild-type and albino plants were detected in the multiple shoot induction, callus formation from the explants and the plantlets regeneration from calli. In addition, albino plants have a similar organogenesis Pattern to that of the wild-type in the media with different combinations of NAA (0 to 5.0mg/L) and BAP (0 to 5.0mg/L) treatment. These results indicate that the albino mutant has the same normal regeneration ability as that of wild-type, although the mutant has lost functions in photosynthesis, such as pigmentation.

• Korean Journal of Plant Tissue Culture
• /
• v.22 no.2
• /
• pp.89-93
• /
• 1995

Regeneration and growth of bulblets from bulblet-derived bulb-scale segment of Lilium longiflorum (cv Georgia) were investigated. Bulblets were initiated on bulb scales taken from bulblets on MS medium containing 0.05 mg/L 2,4D with 3% sucrose or 0.02 mg/L 2,4D with 9% sucrose. Benzyladenine promoted the differentiation of bulblets but inhibited the growth of differentiating bulblets. The growth of bulblet was promoted by supplying 1/2 strength 1/2 NH$_4$NO$_3$ concentration in MS medium containing 12% sucrose.

• Korean Journal of Plant Tissue Culture
• /
• v.22 no.4
• /
• pp.207-211
• /
• 1995

Immature zygotic embryos from immature seeds of Wasabia japonica (cv Dalma) were isolated and cultured on modified MS medium supplemented with 2,4-D, IAA, and BA. Immature zygotic embryos were classified into torpedo shape and cotyledon stage. The highest rates of callus formation were obtained of 1.0mg/L IAA(torpedo stage, 90.0%)and 1.0mg/L 2,4D plus 0.1mg/L BA(cotyledany stage,84.3%). Somatic embryos after 60 days of culture. These numerous somatic embryo could be seperated and subcultured on the same media for further propagation. After 90 days of culture, most somatic embryos were developed well organized embryos which were able to produce into whole plants.

• Korean Journal of Agricultural Science
• /
• v.7 no.2
• /
• pp.65-76
• /
• 1980

In order to determine the effects of cytokinin and auxin on organ formation from tissue of garlic cloves, leaf blades and basal tissues contained meristem of garlic (Allium sativum L.) cloves harvested in 1979 (old cloves) and 1980 (new cloves) were explanted on a MS medium contained various levels of BA ($N^6$-benzyl amino purine), NAA (naphthalene acetic acid), and 2, 4-D (2, 4-dichlorophenoxyacetic acid). And some of the new cloves were explanted on a media contained BA and NAA after chilling treatment at $4^{\circ}C$ for 10, 20, 30 and 40 days. 1. In a culture of leaf blades of old cloves, shoots were differentiated on a medium supplemented with 2mg/l of BA and NAA. 2. Callus was grown as a quite straw-coloured globular mass on a medium contained 0.2 or 2mg/l 2.4-D. 3. As subcultures of globular calli, shoots and roots were differentiated on a medium contained 2mg/l BA and 0.5 or 1 mg/l NAA, whereas no shoots was shown on a conterol. 4. Shoots were differentiated in a culture of leaf blades of new cloves, but they were not in an old cloves in control, and better effect was shown on a medium contained 2mg/l BA and 1mg/l NAA. However shoots were no differentiated from leaf blades chilled at $4^{\circ}C$ for 30 or 40 days at the same condition. 5. Large numbers of adventitious shoots could be obtained from basal region of garlic cultured on a medium contained 1mg/l BA and 4mg/l NAA, or 2mg/l BA and 2mg/l NAA.