• Title/Summary/Keyword: 기간분리

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Effects of 20-hydroxyecdysoneon Adult Development of Diapausing Fall webworm (Hyphantria cunea Drury) Pupae (휴면중인 미국흰불나방 번데기의 성충발육에 미치는 20-hyderoxyecdysone의 영향)

  • Seung Lyeol Gha;Kyung Saeng Boo
    • Korean journal of applied entomology
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    • v.34 no.4
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    • pp.287-294
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    • 1995
  • Studies were carried out to elucidate the efficacy of exogeneous 20-hydroxyecdysone treatment on terminating pupal diapause in the fall webworm. Hyphantria cunea Drury. And the difference was also investigated between normal adult development and post-diapause development after 20-hydroxyecdysone treatment. In the diapause termination rate of pupae treated with 20-hydroxyecdysone after storage for various periods at 16L:8D and $25\pm$$1^{\circ}C$, at the highest rate was observed from the group stored for the longest period and the lowest rate from those stored for 1.5 months. The time needed for adult emergence was inversely proportional to the chilling (at $0\pm$$1^{\circ}C$) period, and the longer its exposure period at low temperature, the higher its sensitivity to 20-hydroxyecdysone treatment. Pupal diapause duration was almost the same, regardless of storage period in the total darkness or at the photoperiod of 16L:8D, and also they successfully emerged to adult even without any experience at low temperature. The oxygen consumption rate in normally developing pupae showed nearly a typical U-form. But, that of diapausing pupae treated with 20-hydroxyecdysone slowly increased and jumped 14 days after the treatment. Pupal diapause began before formation of adult tissues, an a timing of adult tissue formation coincided with ascending timing of the metabolic rate in both normally developing pupae and diapausing pupae treated with 20-hydroxyecdysone. The diapausing pupae treated with 20-hydroxyecdysone was similar to normally developing pupae in band patterns of proteins from haemolymph or fat body.

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An investigation of Residential Overcrowding of Three Asian Ethnic Groups in the US (이중생잔모형을 이용한 아시안 이민자들의 주거밀도 변화추이와 주거과밀 결정요인에 관한 연구)

  • 이성우;조중구;류성호
    • Korea journal of population studies
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    • v.25 no.2
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    • pp.163-192
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    • 2002
  • Residential overcrowding, also called density, measured as more than one person per room, is a important variable as a principal indicator of inadequate housing. We investigated how immigrants in the US are assimilated to the host society through a lens of housing density. We estimated the probability of living in overcrowded housing of Korean, Chinese and Japanese immigrants armored with the 1980 and 1990 Public Use Microdata Sample(PUMS, A, 5%) in the US. Along with economic effects, we also consider life-cycle effects on the indicator. We applied "double cohort" method that enables duration of immigration effects to be separated from aging effects, which captures family size fluctuations due to life-cycle effects. The study found that cohort trends sharply changed during 10years. The 1970's immigrants are more likely to live in overcrowded housing than the pre-1970 immigrants. The pre-1970 immigrants are more likely to live in overcrowded housing than native-born persons. This may be explained by different assimilation processes driven by the disparities of individual human capitals or cultural differences among the ethnic groups. Especially, Korean experienced a sharp decline in overcrowding between 1980 and 1990. We also found that the major determinants that affect the level of housing density are years since migration, income, and gender. The present study concluded with some future studies related to the Korean immigrants abroad.ts abroad.

Differences in Chemical Composition of Soybean Seeds with Different Usages and Storage Durations (저장기간과 용도가 다른 대두종실의 화학성분 차이)

  • 성락춘;박세준;정혜승;김영배
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.39 no.4
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    • pp.382-388
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    • 1994
  • For the comparision of the differences in dry matter weight and the chemical composition of soybean seeds in relation to utilization styles and storage durations, the seed coats and cotyledons including embryo axis separated from the seeds of 3 recommended cultivars stored for 3 years and of 5 recommended cultivars and 2 local varieties with black seed coat and green cotyledons were observed in dry weight, germination percentage, protein, oil, soluble sugar, oilgosaccharide, and starch. In the seeds stored for 3 years, dry weight and germination rate were decreased distinctively. This decrease of seed dry matter resulted from the decrease of the cotyledon weight, and the ratio of seed coat to whole seed was increased. For the change of chemical compositions by the decrement of seed dry weight during the storage, protein, oil, and carbohydrate contents of the seed coat were decreased, and protein content of the cotyledon was markedly decreased in the seeds stored for 3 years. The ratio of seed coat to whole seed was higher in small seed than large seed cultivars. In the local varieties, protein and oil contents were 43.59% and 18.25% in the cotyledons, respectively, which were lower than those of recommended cultivars. But starch and sugar contents were 5.70% and 11.58%, respectively, which were higher than those of recommended cultivars. Sucrose, raffinose and stachyose contents were also higher in the cotyledon of the local varieties.

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Experimental Analysis on Filtration-Permeation: Influence of the Type and Sheets of Filter Media, and Filtration Pressure (여과-투과에 대한 실험적 분석: 여과매체의 종류, 여과매체의 매수, 여과압력의 영향)

  • Yim, Sung-Sam;Song, Yun-Min
    • Korean Chemical Engineering Research
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    • v.50 no.2
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    • pp.317-327
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    • 2012
  • The average specific cake resistance, the most important indicator for cake filtration and solid-liquid separation, is measured by filtration experiment. But the exact value is difficult to measure because of the other influences such as sedimentation during filtration. This study, a little more stable method named filtration-permeation is proposed for measuring average specific cake resistance. The filtration-permeation is composed of permeation of particle eliminated water through pre-formed cake by filtration. Using 1 wt% calcium carbonate suspension, the filtration-permeation experiments were performed for 8 kinds of filter media at the conditions of 0.5 atm and 0.2 atm, 1 and 3 sheets of filter media. At each specific condition, three to five times filtration-permeation were accomplished. As a result, stable permeation speed is measured. According to this experimental result, the characteristics of permeation and the effect of sedimentation are analyzed with Ruth's equation. The one way analysis of variance (one way ANOVA) is applied to the average specific cake resistances of filtration and permeation obtained with the selected three kinds of filter media. The average specific cake resistances between 0.5 atm and 0.2 atm by filtration do not distinguished, but those by permeation is perfectly distinguished. The experimental results during permeation have a very narrow distribution than that measured during filtration. The analysis of filtration experiments, it was verified that the resistance of filter medium by traditional method is of no significance. Finally, the migration of small particles through the medium composed of fiber glass at low pressure was studied.

Changes in Aflatoxin and Flavor Components of Traditional Sigumjang (전통 시금장 발효기간별 Aflatoxin 및 휘발성 향기성분의 변화)

  • Son, Dong-Hwa;Choi, Ung-Kyu;Kwon, O-Jun;Im, Moo-Hyeog;Dahn, Kyeong-Nyeo;Cha, Won-Seup;Cho, Young-Je;Chung, Yung-Gun
    • Korean Journal of Food Science and Technology
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    • v.32 no.1
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    • pp.181-186
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    • 2000
  • This study was conducted to investigate various components and aflatoxin of sigumjang during fermentation time. The contents of moisture were decreased during fermentation time. The contents of protein, fat and ash were $19.8{\sim}20.8%,\;1.3{\sim}1.6%$ and $3.1{\sim}3.2%$, respectively. The pH was gradually acidized. The result of measurement of surface color showed: L-value, $29.45{\sim}30.94\;a-value,\;+3.01{\sim}+3.63;\;b-value,\;+5.39{\sim}+6.34$. Aflatoxin was not detected during fermentation. Among 81 kinds of flavor components identified in sigumjang, esters was most in number followed by acids, aldehydes, alcohols and phenols.

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Effect of Crude Polysaccharide Isolated from Mageoli Mash under Different Fermentation Conditions on Immune-Stimulating Activity (발효 조건이 막걸리 술덧 유래 다당의 면역자극 활성에 미치는 효과)

  • Rhee, Young Kyoung;Nam, So-Hyun;Kim, Hye-Ryun;Cho, Chang-Won;Lee, Young-Chul;Kim, Young-Chan;Hong, Hee-Do
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.43 no.12
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    • pp.1835-1842
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    • 2014
  • Various microorganisms are involved in the fermentation of Mageoli, Korean traditional liquor. Among them, the most predominant microorganism is yeast, including Saccharomyces cerevisae. This study investigated the effects of crude polysaccharides separated from Mageoli mash (CP-M) on activation of peritoneal macrophages in cell culture medium. Four types of yeasts, S. cerevisae 89-1-1, 98-2, 268-3, and 113-4, were used. When peritoneal macrophages were treated with $10{\mu}g/mL$ of CP-M fermented with S. cerevisae 113-4, the concentration of nitric oxide (NO) in the medium was highest ($33.3{\mu}M$). The concentrations of IL-6 and -12 were also highest at 116.3 pg/mL and 59.8 pg/mL, respectively. In the case of CP-M cultured at 15, 20, and $30^{\circ}C$ after inoculation with S. cerevisae 113-4 and fermented at different temperatures, production of NO and IL-6 by peritoneal macrophages did not change compared to the control. For CP-M obtained after fermentation at $25^{\circ}C$, however, NO concentration increased 2.7~3.3 fold and IL-6 concentration by 5.7 fold compared to the control. Furthermore, the effect of fermentation period on expression of CP-M functionality was examined. NO production by CP-M of the 5th day mash was highest, increasing 2.2 fold compared to 0-day CP-M. However, there were no significant differences in concentration of IL-6 or -12 according to different fermentation periods, although CP-M showed a large decrease after 10 days. The results show that fermentation conditions with the highest activity were observed in CP-M inoculated with S. cerevisae 113-4 and fermented at $25^{\circ}C$ for 5 days. CP-M consisted of 78.6% neutral sugar, 11.6% acidic polysaccharide, and 9.8% protein. In particular, the sugar composition of neutral sugar consisted of mannose (47.8%), glucose (29.6%), and galactose (12.7%). Based on the results, CP-M is assumed to be an extracellular polysaccharide originating in yeast with high mannose content.

Characterization of MACS Isolated Cells from Differentiated Human ES Cells (인간 배아줄기세포로부터 분화된 세포에서 MACS 방법을 이용하여 분리한 세포의 특성에 대한 연구)

  • Cho, Jae Won;Lim, Chun Kyu;Shin, Mi Ra;Bang, Kyoung Hee;Koong, Mi Kyoung;Jun, Jin Hyun
    • Clinical and Experimental Reproductive Medicine
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    • v.33 no.3
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    • pp.171-178
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    • 2006
  • Objective: Human embryonic stem (ES) cells have a great potential in regenerative medicine and tissue engineering. The human ES cells could be differentiated into specific cell types by treatments of growth factors and alterations of gene expressions. However, the efficacy of guided differentiation and isolation of specific cells are still low. In this study, we characterized isolated cells from differentiated human ES cells by magnetic activated cell sorting (MACS) system using specific antibodies to cell surface markers. Methods: The undifferentiated hES cells (Miz-hESC4) were sub-cultured by mechanical isolation of colonies and embryoid bodies were spontaneously differentiated with DMEM containing 10% FBS for 2 weeks. The differentiated cells were isolated to positive and negative cells with MACS system using CD34, human epithelial antigen (HEA) and human fibroblast (HFB) antibodies, respectively. Observation of morphological changes and analysis of marker genes expression were performed during further culture of MACS isolated cells for 4 weeks. Results: Morphology of the CD34 positive cells was firstly round, and then it was changed to small polygonal shape after further culture. The HEA positive cells showed large polygonal, and the HFB positive spindle shape. In RT-PCR analysis of marker genes, the CD34 and HFB positive cells expressed endodermal and mesodermal genes, and HEA positive cells expressed ectodermal genes such as NESTIN and NF68KD. The marker genes expression pattern of CD34 positive cells changed during the extension of culture time. Conclusion: Our results showed the possibility of successful isolation of specific cells by MACS system from undirected differentiated human ES cells. Thus, MACS system and marker antibodies for specific cell types might be useful for guided differentiation and isolation of specific cells from human ES cells.

Frequency of Candida Strains Isolated from Candidiasis Patients at A Tertiary Hospital over the Last 10 Years (최근 10년 동안 일개 상급종합병원의 칸디다혈증 환자에서 분리된 칸디다 균종의 빈도)

  • Hwang, Yu-Yean;Kang, On-Kyun;Park, Chang-Eun;Hong, Sung-No;Kim, Young-Kwon;Huh, Hee-Jae;Lee, Nam-Yong
    • Korean Journal of Clinical Laboratory Science
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    • v.54 no.2
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    • pp.110-118
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    • 2022
  • Candidemia is a major cause of nosocomial infections resulting in increased morbidity and mortality. It remains a serious risk in inpatients and increases medical treatment costs. From 2009 to 2018, Candida strains (3,533) isolated from blood culture tests at the S Hospital were analyzed according to the period, year, sex, age, ward, etc. During the entire period, 54,739 of 717,996 blood culture tests showed a positive rate (7.6%) and the Candida isolation rate was 3,533 (6.4%) out of 1,036 patients. Among the Candida isolates, C. albicans was most common (33.8%), followed by C. tropicalis (28.6%), C. glabrata (19.8%), C. parapsilosis (7.8%), and C. krusei (4.0%). In early (2009~2013)/late (2014~2018) isolation, C. tropicalis decreased by 3.8% and C. glabrata increased by 3.4%. After 50 years of age, the higher the separation frequency. C. parapsilosis (31.3%) in 1~10s, C. tropicalis (30.3%) and C. glabrata (27.6%) in 41~50s, and C. tropicalis (28.6%) in 80s are relatively frequent. has been separated C. krusei was isolated in a relatively high proportion from females (60.9%). Therefore, a systematic and continuous nosocomial infection control system should be established for appropriate treatment as per antifungal treatment guidelines. The system should continuously monitor the distribution of Candida species and provide rapid identification results.

Effects of the Phospholipid separated from Duck Egg Oil on the Rat Brain (청둥오리 난황유에서 분리한 인지질이 쥐 뇌에 미치는 영향)

  • Jung In-Teak;Moon Yoon-Hee;Ryu Beung-Ho
    • The Korean Journal of Food And Nutrition
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    • v.19 no.1
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    • pp.62-69
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    • 2006
  • This study was performed to investigate the biocogical activity of the phospholipid separated and purified from duck egg yolk as functional brain food. A quantitative restriction of maternal diet was given to Sprague-Dawely rats during lactation. Animals were separated into three groups, such as control, duck egg phospholipid(PL) or pig-brain phospholipid additions groups. And ad libitum during lactation was given to control groups. All of the offsprings at 3 weeks of age fed a diet ad libitum for 4 weeks of rehabilitation. Body weight and brain weight and protein, phosphplipid, and cholesterol were determined on offsprings of each of group sacrificed at 0, 1, 2, 3, 5 and 7 weeks of age, sacrified body weight of duck egg phospholipid and pig brain phospholipid group were significantly higher than the control group. Body weight and brain weight after a recovery period were significantly at the control group. However, protein, phospholipid and cholesterol contents were significantly high than the control group. Response latency in the passive avoidance task was the lowest in the ${\beta}$-amyloid group and highest in the phospholipid group. It is consequently suggested that phospholipid supplement may be effective in memory improvement.

Stream Discharge Estimation by Runoff Component Analysis on the Control Point (유출성분 분석에 의한 제어지점의 유출량 산정)

  • Lee, Sang-Jin;Hwang, Man-Ha;Lee, Bae-Sung;Park, Joo-Seong
    • Proceedings of the Korea Water Resources Association Conference
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    • 2006.05a
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    • pp.785-789
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    • 2006
  • 유역 수자원의 효율적인 관리 및 배분을 위해서는 세밀한 강우-유출관계의 규명이 무엇보다 중요하다. 이를 위해서는 먼저 하천 유출지점의 정확한 유량정보가 획득되어야 하며, 장기간에 걸쳐 신뢰성 있는 유량자료의 확보는 더욱 중요한 사항이다. 본 연구에서는 하천에서 관측된 유량자료를 장기간(1983년${\sim}$2004년)에 걸친 유출성분으로 분리하는 기법을 활용하여 제어지점의 유출량을 검증하였다. 유량자료를 출구지점의 관측유량$(Q_{ob})$을 회귀수$({\alpha}Q_e)$, 상류유입량$(Q_{up})$ 및 관측강우-유출량$({\beta}Q_{Rain})$의 성분으로 구분하여 산정하는 방식으로 유출량을 추정하였다. 여기서, 회귀수$({\alpha}Q_e)$란 유역 및 하도내 용수이용량의 회귀수, 상류유입량$(Q_{up})$은 상류 유출 제어지점의 관측유량으로 대청댐 방류량, 관측강우-유출량$({\beta}Q_{Rain})$은 유역내 강우에 의한 자연유출량이다. 여기서 사용된 수문기초자료는 대청댐 방류량, 대전 및 청주권 취수량, 강우에 의한 자연유출량, 공주관측유량 등으로 각 성분별로 생성된 일자료를 이용하여 공주지점의 월별, 분기별, 년도별 유출량을 산정하였다. 이 결과는 금강유역에 이미 구축되어있는 SSARR모형을 기반으로 한 RRFS(Rainfall Runoff Forecasting System, 유출예측 시스템)의 결과 및 관측치와 비교되었다. 계산결과 RRFS에 의한 유출량과 대청-공주구간의 유출성분분리에 의한 유출량은 관측값과 전반적으로 근사함을 확인하였으며, 검증지점의 정확한 유출율을 산정할 수 있다면, 관측자료의 연속성 및 신뢰도를 파악하는 척도를 제공할 수 있을 것으로 판단된다.측결과 있는 대상유역에 대한 적용이 요구된다.-Moment 방법에 의해 추정된 매개변수를 사용한 Power 분포를 적용하였으며 이들 분포의 적합도를 PPCC Test를 사용하여 평가해봄으로써 낙동강 유역에서의 저수시의 유출량 추정에 대한 Power 분포의 적용성을 판단해 보았다. 뿐만 아니라 이와 관련된 수문요소기술을 확보할 수 있을 것이다.역의 물순환 과정을 보다 명확히 규명하고자 노력하였다.으로 추정되었다.면으로의 월류량을 산정하고 유입된 지표유량에 대해서 배수시스템에서의 흐름해석을 수행하였다. 그리고, 침수해석을 위해서는 2차원 침수해석을 위한 DEM기반 침수해석모형을 개발하였고, 건물의 영향을 고려할 수 있도록 구성하였다. 본 연구결과 지표류 유출 해석의 물리적 특성을 잘 반영하며, 도시지역의 복잡한 배수시스템 해석모형과 지표범람 모형을 통합한 모형 개발로 인해 더욱 정교한 도시지역에서의 홍수 범람 해석을 실시할 수 있을 것으로 판단된다. 본 모형의 개발로 침수상황의 시간별 진행과정을 분석함으로써 도시홍수에 대한 침수위험 지점 파악 및 주민대피지도 구축 등에 활용될 수 있을 것으로 판단된다. 있을 것으로 판단되었다.4일간의 기상변화가 자발성 기흉 발생에 영향을 미친다고 추론할 수 있었다. 향후 본 연구에서 추론된 기상변화와 기흉 발생과의 인과관계를 확인하고 좀 더 구체화하기 위한 연구가 필요할 것이다.게 이루어질 수 있을 것으로 기대된다.는 초과수익률이 상승하지만, 이후로는 감소하므로, 반전거래전략을 활용하는 경우 주식투자기간은 24개월이하의 중단기가 적합함을 발견하였다. 이상의 행태적 측면과 투자성과측면의 실증결과를 통하여 한국주식시장에 있어서 시장수익률을 평균적으로 초과할 수 있는 거래전략은 존재하므로 이러한 전략을 개발 및 활용할 수 있으며, 특히, 한국주식시장에 적합한 거래전략은 반전거래전략이고, 이 전략의 유용성은 투자자가 설정한 투자기간보다 더욱 긴

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