• Journal of the Korea Academia-Industrial cooperation Society
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• v.20 no.12
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• pp.340-347
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• 2019

This study investigated the brewing properties of rice beer made with yeast isolated from traditional whole wheat nuruk. The experimental method was followed by alcohol fermentation and quality analysis for six months after separating the yeast from nuruk. The yeasts isolated from traditional nuruk showed normal fermentation characteristics, like those of commercial yeast, in terms of alcohol production capability, sugar content, and pH reducing power. Especially, the yeast (KCCM 301) isolated from whole wheat nuruk showed higher contents of glycogen and trehalose than that of commercial yeast, and so KCCM 301 yeast has excellent yeast vitality. Meanwhile, the yeast (KCCM 90301) isolated from traditional Nuruk produced significantly higher alcohol and ester contents than that of commercial yeast. This has a positive effect for supplementing the taste and aroma of rice beer. In conclusion, the yeasts isolated from whole wheat nuruk showed the general alcohol fermentation pattern and aroma content of rice beer. These yeasts seem to be effective in strengthening the flavor of rice beer.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.6 no.3
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• pp.31-42
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• 1986

The probabilistic model was developed to give a spatial simlation of precipitation series to solve the problem of future need of water resources. The simulation of daily precipitation series at the sub-base stations was induced from the spatial structure of rainfall occurrence probability between the base station and the sub-base stations in the watershed. In this study Hadong was chosen as the base station in Seomjin river basin and Imsil, Boseong, Soonchang, Dongbok, and Gurye were also selected as the sub-base stations. The results of this study are as follows; 1) The separation technique of spatial precipitation state showed effectiveness in the spatial simulation method because the occurrence probability by each precipitation state (Wet-Wet, Dry-Wet, Wet-Dry, and Dry- Dry system) represented the stable value. 2) The daily precipitation series of the sub-base stations which were simulated from those of the base station showed that the simulated annual mean precipitations were similar to the observed data, but the precipitations in summer were decreased slightly. 3) The correlogram and power spectrum of the simulated monthly precipitation for the sub-base stations showed those of the observed sample with good agreement.

• Proceedings of the Korea Water Resources Association Conference
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• 2019.05a
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• pp.349-353
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• 2019

우리나라는 전 국토의 70%가 산지이고 하천경사가 다른 나라에 비해 상대적으로 급하여 홍수 관리에 매우 불리한 조건을 가지고 있으며, 특히 홍수기간의 집중호우 및 돌발홍수는 인명과 재산의 막대한 피해를 입히고 있다. 최근은 기후변화로 인하여 극심한 홍수, 가뭄 등 재해의 발생빈도가 증가하는 추세로 기후변화의 영향을 최소화할 수 있는 수재해 방재관리가 필요한 상황이다. 중 대하천의 경우에는 비교적 수재해 방재관리가 잘 이루어지고 있으나, 소하천(일부 중하천 포함)의 경우에는 취약한 구조를 보이고 있다. 특히 홍수기간(7월~9월)의 인명과 재산의 피해는 주로 소하천 위주로 발생하고 있으며, 사전 사후의 체계적인 대응이 이루어지지 못하고 있다. 수재해 방재관리를 위해서는 일차적으로 수문자료의 획득에 있으며, 그 이후 해당유역에 적합한 수재해 대응을 위한 체계적인 방법론과 방재시스템 개발 운영이 수반되어야 안전한 방재관리를 할 수 있다. 따라서 수재해 방재관리 체계를 구축하기 위해서는 중 소규모 유역 단위를 대상으로 지속적이고 신뢰성 있는 자료의 획득과 축적이 중요하므로 중 소규모 유역 단위의 대표성 있는 시험유역의 운영은 매우 의미가 있다고 볼 수 있다. 본 논문에서는 한국건설기술연구원에서 운영하는 설마천 시험유역(유역면적 $8.48km^2$, 유로경사 2.15%, 경기도 파주시 적성면 소재)의 신뢰성 높은 2018년 관측자료를 이용하여 강우특성, 유출특성, 증발산량과 지하수 함양량 산정 등 수문특성을 분석하였으며, 2017년 관측결과와 비교하였다. 강우특성 분석으로는 호우사상 분리, 주요 호우사상 분석, 지속기간별 최대강우량, 시간분포 등이 있다. 2018년은 2017년보다 최대 강우지속기간은 작게, 평균 강우지속기간은 크게 나타나며, 최대 강우강도와 평균 강우강도도 크게 나타나는 호우의 특징을 보인다. 2018년 지속기간별 최대강우량의 경우 지속기간 1시간까지는 2017년과 유사한 패턴을 보이나 그 이후는 많은 강우량을 보인다. 2018년의 하천유출률은 58.1%로 2017년 연간 유출률인 64.8% 보다는 적은 유출률을 보인다. 이의 원인으로는 연간 총강우량은 2017년보다 397.9mm 많으나 2018년의 7월~8월 집중호우기간의 강우량이 2017년 7월~8월 강우량보다 적어 하천유출에 적게 기여한 결과로 판단된다. 2018년의 증발산량(555.8mm)과 지하수 함양량(32.0mm)은 2017년의 증발산량(328.8mm)과 지하수 함양량(24.8mm)보다 크게 나타나는 것으로 분석되었다. 이와 같이 산정된 수문자료는 수재해 방재와 유역의 물순환 과정 규명을 위한 기초자료로 매우 유용하게 활용될 수 있으며, 수재해 방재관리를 위한 의사결정 과정에 중요한 역할을 할 수 있으므로 지속적인 시험유역의 운영은 매우 필요하다.

• Applied Biological Chemistry
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• v.49 no.1
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• pp.7-14
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• 2006

This study was carried out to examine the quality characteristics of soybean pastes made of Meju with mold-producing pretense isolated from traditional Meju. The changes in moisture content, enzyme activity (amylase, protease, lipase), reducing sugar, amino-type nitrogen contents and anti-oxidant activity were investigated during the aging period. The moisture contents decreased gradually with time. Amylase activity decreased during the aging period while pretense and lipase activities increased until 30-45 days of aging, but decreased thereafter, Especially pretense activity in soybean paste with mold was greater than that in the control. Amino-type nitrogen and ammoniacal nitrogen contents increased, but reducing sugar contents decreased with time. Amino-type nitrogen contents were greater in soybean paste made of Meju with isolated mold than those in the control. Antioxidative activity was also confirmed in soybean paste.

• Journal of the East Asian Society of Dietary Life
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• v.11 no.5
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• pp.393-399
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• 2001

Effects of various types of salts (commercial low salt, sea salt, refined salt, and bamboo salt) and concentrations (0, 3.0 and 5.0% ) on the growth of yeasts isolated from Kimchi were investigated. The isolated yeasts used in the study are as follows : Saccharomyces cerevisia, Sporobolomyces albo-rubescens, Issatchenkia orientalis Cryptococcus luteolous, Ustilago maydis, Candida humilis, Pichia onychis, Cadida nitratophila, and Pichia jadinii. The growths of the yeasts were inhibited against each salt concentration. The growths of Candida sp. isolated from the later stage of fermentation was strongly inhibited against 5% concentration of salt. On the other hand the induction phase of Issatchenkia orientalis isolated at the early stage of fermentation was the quickest among all the tested yeasts. Among the salts, bamoo salt was found to be strongest inhibitor of the growth of yeasts.

• The Korean Journal of Mycology
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• v.15 no.3
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• pp.169-174
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• 1987

The studies on the screening and properties of Raw Starch Saccharifying Microorganism were as follows;Apotent mold strain was selected and screened to digest raw starch, which was classified as a strain of Aspergillus sp. SN-871. The crude enzyme production was maximized when grown on wheat bran media for 5 days at $30^{\circ}C$ and pH 4.0. The stable range of pH was 2 to 5.

• Journal of the Korean GEO-environmental Society
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• v.9 no.5
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• pp.29-36
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• 2008

The shotcrete is a NATM technique as a major tunnel support for ground stability after tunnel excavation. Instead of a general concrete lining method, it is a trend for curtail of construction periods and reduction of construction expenses that required to use of the permanent shotcrete lining. This high-strength shotcrete is required to use as a permanent shotcrete lining. This brought out the solution of environmental pollution and harmfulness to human. Accordingly, in this study specimens for strength measurement were made to develop shotcrete possible to develop materials in early with cement mineral accelerator as NATM method construction. It was compared with existing shotcrete material, unconfined compression test, flexural strength test, antiwashout underwater test were experimented. The fish poison test was experimented to evaluate an influence of environment. As a results of the test, unconfined compressive strength and flexural strength were equivalent with 28-curing day strength of existing material. An antiwashout of research subject material was revealed excellently in antiwashout Underwater test. As a results of the fish poison, an evaluation research subject material was founded more environmentally friendly than existing shotcrete.

• Microbiology and Biotechnology Letters
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• v.39 no.3
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• pp.218-223
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• 2011

To obtain eicosapentaenoic acid (EPA)-producing bacteria, some 600 strains of bacteria were isolated from Antarctic sediment and marine organisms during the summer expedition of 1999-2000 and 7 EPA-producing bacteria were obtained through screening with TLC and GC. A strain designated as L93 showed the highest EPA production, which was gram-negative, rod-shaped bacterium. L93 strain was identified as Shewanella sp., from the sequence analysis of 16S rDNA. Optimal conditions temperature and pH for the growth and EPA production were about $4^{\circ}C$ and pH 7. In addition, its production was optimized by 50%(w/v) sea salt. We establish the optimal production system to produce about 320 mg per liter by using this optimal EPA production conditions. EPA-methyl ester was purified from cultured L93 strain to a purity of higher than 97% and typical purification yield is greater than 72% of the input amount via urea complexation and HPLC.

• Water for future
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• v.29 no.1
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• pp.129-139
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• 1996

It is very important to select appropriate distributions for hydrological data in planning and designing hydraulic structures. Also, it is necessary to check whether the selected distribution reproduces the statistical characteristics of the real data. In this study, the parameters of the two- and three-parameter gamma, two- and three-parameter lognormal, Gumbel, two- and three-parameter log-Gumbel, GEV, log-Pearsonn type III, two- and three-parameter Weibull, four- and five-parameter Wakeby distributions were estimated for the rainfall data of 22 sites in Korea with 7 different durations based on the methods of moments, probability weighted moments, and maximum likelihood. And the validity conditions were checked for the estimated parameters. The separation effect for each distribution was examined throught 10,000 simulations using the estimated parameters. As results, the separation effect was the smallest: log-Pearson type III for moment method, log-Pearson type III and GEV for probability weighted moment method, and GEV for maximum likelihood method. However, it is large for the two-parameter distributions.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.9 no.3
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• pp.800-806
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• 2008

The strain HK-12 was enriched and isolated from naturally fermented soybean for the production of fibrinolytic enzyme and the proteome of this enzyme induced during the incubation period was analyzed. The activity of fibrinolytic enzyme derived from supernatants of the HK-12 culture was performed by fibrin plate method for solid fibrinolytic activity. As the result, the fibrinolytic activity of HK-12 grown on the nutrient agar media was about 2.3 times greater than that of plasmin used as standard. The purified enzyme was prepared by a series of purification process including ammonium sulfate precipitation, DEAE-cellulose, Sephadex chromatography. The molecular weight of the enzyme was determined to approximately 23kDa with SDS-PAGE. In order to examine which strain HK-12 proteins increased or decreased during the incubation period, 2-DE analysis was performed. Protein spot #1 significantly expressed on the 2-DE gel of bacteria cultivated for 36-hrs was analysed. As the result of protein sequence analysis using MALDI-TOF MS, one protein was identified as serine protein kinase (PrkA).