Browse > Article

Optimal Condition for Eicosapentaenoic Acid Production and Purification from Psychrophillic Marine Baterium Shewanella sp. L93  

Mo, Sang-Joon (Division of Bioscience and Bioinformatics, Myongji University)
Hong, Hye-Won (Korea Drug Test Laboratory)
Bang, Ji-Heon (Department of Marine Biotechnology, Anyang University)
Cho, Ki-Woong (Department of Marine Biotechnology, Anyang University)
Publication Information
Microbiology and Biotechnology Letters / v.39, no.3, 2011 , pp. 218-223 More about this Journal
Abstract
To obtain eicosapentaenoic acid (EPA)-producing bacteria, some 600 strains of bacteria were isolated from Antarctic sediment and marine organisms during the summer expedition of 1999-2000 and 7 EPA-producing bacteria were obtained through screening with TLC and GC. A strain designated as L93 showed the highest EPA production, which was gram-negative, rod-shaped bacterium. L93 strain was identified as Shewanella sp., from the sequence analysis of 16S rDNA. Optimal conditions temperature and pH for the growth and EPA production were about $4^{\circ}C$ and pH 7. In addition, its production was optimized by 50%(w/v) sea salt. We establish the optimal production system to produce about 320 mg per liter by using this optimal EPA production conditions. EPA-methyl ester was purified from cultured L93 strain to a purity of higher than 97% and typical purification yield is greater than 72% of the input amount via urea complexation and HPLC.
Keywords
Eicosapentaenoic acid; psychrophillic; marine bacterium; Shewanella sp. L93;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
Times Cited By SCOPUS : 0
연도 인용수 순위
1 Taketama, H., K. Iwamoto, S. Hata, H. Takno, and T. Matsunaga. 1996. DHA enrichment of rotifers: A simple two-step culture using the unicellula algae chlorella regularis and Isochrysis galbana. J. Mar. Biotechnol. 3: 244-247.
2 Yazawa, K., K. Araki, N. Okazaki, K. Watanabe, C. Ishikawa, A. Inoue, N. Numao, and K. Kondo. 1988. Production of eicosapentaenoic acid by marine bacteria. J. Biochem. 103: 5-7.
3 Dyeberg, J., H. O. Bang, E. Stoffersen, S. Moncada, and J. R. Vane. 1978. Eicosapentaenoic acid and prevention of thrombosis and atherosclerosis? Lancet ii: 117-119.
4 Kelly, J. F. 1991. The metabolic role of n - 3 polyunsaturated fatty acids: relationship to human disease. Comp. Biochem. Physiol. A Comp. Physiol. 98: 581-585.   DOI
5 Lopoz Alonso, D., E. Molina Grima, J. A. Sunclez Perez, J. L. Gracia Sanchez, and F. Gracia Camacho. 1992. Fatty acid variation among different isolates of a single strain of Isochrysis galbana. Phytochemistry 31: 3901-3904.   DOI   ScienceOn
6 Lane, D. J. 1991. Nucleic acid techniques in bacterial systematic, pp. 115-175. John Wiley and Sons, New York, U.S.A.
7 Lee, S. J., P. S. Seo, C. H. Kim, O. Kwon, B. K. Hur, and J. W. Seo. 2009. Isolation and characterization of the eicosapentaenoic acid biosynthesis gene cluster from Shewanella sp. BR-2. J. Microbiol. Biotechnol. 19: 881-887.   DOI
8 Lee, W. H., K. W. Cho, S. Y. Park, K. S. Shin, D. S. Lee, S. K. Hwang, S. J. Seo, J. M. Kim, S. Y. Ghim, B. H. Song, S. H. Lee, and J. G. Kim. 2008. Identification of psychrophile Shewanella sp. KMG427 as an eicosapentaenoic acid producer. J. Microbiol. Biotechnol. 18: 1869-1873.
9 Pettinella, C., S. H. Lee, F. Cipollone, and I. A. Blair. 2007. Targeted quantitative analysis of fatty acids in atherosclerotic plaques by high sensitivity liquid chromatography/tandem mass spectrometry. J. Chromatogr. B 850: 168-176.
10 Salunkhe, D., N. Tiwari, S. Walujksr, and R. Bhadekar. 2011. Halomonas sp. nov., an EPA-producing mesophilic marine isolate from the Indian Ocean. Pol. J. Microbiol. 60: 73-78.
11 Christie, W. W. 1982. Lipid Analysis, Pergamon Press. London.
12 Cho, K. W. and S. J. Mo. 1999. Screening and characterization of eicosapentaenoic acid-producing marine bacteria. Biotechnol. Lett. 21: 215-218.   DOI   ScienceOn
13 Connor, S. L. and W. E. Conner. 1997. Are fish oils benefical in the prevention and treatment of coronary artery disease? Am. J. Clin. Nutr. 66 (suppl): 1020-1031.
14 Dyerberg, J. and H. O. Bang. 1979. Lipid metabolism, atherogenesis, and haemostasis in Eskimos: the role of the prostaglandin-3 family. Haemostasis 8: 227-233.
15 Bang, H. O. and J. Dyerberg. 1972. Plasma lipids and lipoproteins in Gleenlandic west coast Eakimos. Acta Med. Scand. 192: 85-94.
16 Bowman, J. P., S. A. McCammon, D. S. Nichols, J. H. Skerratt, S. M. Rea, P. D. Nichols, and T. A. McMeekin. 1997. Shewanella gelidimarina sp. nov. and Shewanella frigidimarina sp. nov., novel Antarctic species with the ability to produce eicosapentaenoic acid (20:5 omega 3) and grow anaerobically by dissimilatory Fe(III) reduction. Int. J. Syst. Bacteriol. 47: 1040-1047.   DOI   ScienceOn