• Journal of Korean Society of Forest Science
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• v.112 no.1
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• pp.40-56
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• 2023

Tree species within the Populus genus grow rapidly and have an excellent capacity to absorb carbon, conferring substantial ability to effective purify the environment. Poplar breeding can be achieved rapidly and efficiently if a genetic linkage map is constructed and quantitative trait loci (QTLs) are identified. Here, a high-density genetic linkage map was constructed for the control pollinated progeny using the genotyping-by-sequencing (GBS) technique, which is a next-generation sequencing method. A search was also performed for the genes associated with quantitative traits located in the genetic linkage map by examining the variables of height and diameter at root collar, and resilience to insect damage. The height and diameter at root collar were measured directly, while the ability to recover from insect damage was scored in a 4-year-old breeding population of aspen hybrids (Odae19 × Bonghyeon4 F1) established in the research forest of Seoul National University. After DNA extraction, paternity was confirmed using five microsatellite markers, and only the individuals for which paternity was confirmed were used for the analysis. The DNA was cut using restriction enzymes and the obtained DNA fragments were prepared using a GBS library and sequenced. The analyzed results were sorted using Populus trichocarpa as a reference genome. Overall, 58,040 aligned single-nucleotide polymorphism (SNP) markers were identified, 17,755 of which were used for mapping genetic linkages. The genetic linkage map was divided into 19 linkage groups, with a total length of 2,129.54 cM. The analysis failed to identify any growth-related QTLs, but a gene assumed to be related to recovery from insect damage was identified on linkage group (chromosome) 4 through genome-wide association study.

• Journal of Korean Society of Forest Science
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• v.94 no.3 s.160
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• pp.191-196
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• 2005

This studies were conducted to compare ozone sensitivity among half-sib families of Japanese red pine (Pinus densiflora S. et Z.). 90 families were exposed in walk-in type chambers to charcoal-filtered air and 100 ppb ozone for 8 hours daily, 5 consecutive months. We surveyed the foliar injury, Relative DRC (diameter at root collar) growth and relative dry weight after fumigation. After 5 months of exposure, foliar injury ranged from 1.9% at tolerant group to 15.2% at sensitive group. Relative DRC growth rates showed increased 12% at tolerant group, but reduced 23% at sensitive group. Total dry weight increased 17% at tolerant group, otherwise reduced by 33% at sensitive group, relative to the control. The root biomass showed more sensitive than the other part biomass. On the basis of the results of these screening studies, families Kangwon 04, 16, 26, 27 and 73 were determined to be tolerant; Kangwon 40, 52, 83, Gyeonggi 01 and Gyeongbuk 20 to be sensitive to ozone. Considerable genetic variation in ozone responses occurred, as shown by differences in sensitivities among families and among groups. These results indicate that tolerant groups of P. densiflora exhibited a high degree of resistance to ozone pollution injury.

• Journal of the Korean Institute of Landscape Architecture
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• v.45 no.3
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• pp.1-8
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• 2017

The present study aimed to investigate the effect of calcium chloride($CaCl_2$) on the growth and physiological responses of Pinus strobus and the variables that are sensitive to $CaCl_2$. Thus, changes in the visible damage, growth of root collar diameter, plant water content, chlorophyll content and composition, maximum PS II photochemical efficiency, and electron transport rate of P. strobus was analyzed in relation to treatment witih $CaCl_2$. A $CaCl_2$ solution(0.5, 1.0 and 3.0%) was applied in the root zone before leaf unfolding. Leaf browning, defoliation, and drying were observed with $CaCl_2$ application and this pattern was aggravated as the $CaCl_2$ concentration increased and the treatment period became longer. The decrease of growth in root collar diameter and height and leaf water content were observed at $CaCl_2$ 1.0% and 3.0%. The total chlorophyll content indicated that photopigment, PS II photochemical efficiency and electron transport rate significantly decreased at $CaCl_2$ 3.0%. In conclusion, $CaCl_2$ affected leaf water content and led to a decrease of capability in light harvesting and photochemical responses. Also, as a result of the correlation between calcium chloride concentration and growth and physiological response parameters, it was found that the leaf moisture content and the ratio of chlorophyll a and b reflect the damage level of calcium chloride sensitively because their coefficient of determinations were relatively high.

• Journal of Korean Society of Forest Science
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• v.95 no.3
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• pp.323-327
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• 2006

This study was conducted to understand response of damage and tolerance to ozone between ozone tolerant and sensitive families of P. densiflora which had been selected by the based on visual injury and growth. Five individuals were selected in each group, and were exposed to 100ppb ozone for 90 days. Every 30 days after ozone fumigation, diamter at root collar (ORC), superoxide dismutase (SOD) activity, and malondialdehyde (MDA) content were measured. In early state of ozone fumigation tolerant families showed three times higher relative DRC growth rate than sensitive families. And during whole fumigation period growth rate of tolerant families was superior to growth rate of sensitive families. SOD activity in sensitive families was increased 30 days after fumigation, and in accordance with fumigation period extended it was decreased continuously. MDA content in ozone treatment showed higher than in control, and MDA content of tolerant families was higher than that sensitive families. In our results tolerant families has higher antioxidative activity than sensitive families. Therefore tolerant families can restrain lipid peroxidation and damage on physiological activity.

• Korean Journal of Agricultural and Forest Meteorology
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• v.1 no.2
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• pp.97-102
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• 1999

This study was conducted to compare seasonal changes of height, diameter at root collar, biomass and chlorophyll contents of Abies holophylla seedlings grown in Sangju National University Nursery under different light intensities. Four light intensities were adopted using nylon net such as 100% sunlight(1800 $\mu$㏖ m$^{-2}$ s$^{-1}$ ), 75% of sunlight (1350 $\mu$㏖ m$^{-2}$ s$^{-1}$ ), 50% of sunlight (900 $\mu$㏖ m$^{-2}$ s$^{-1}$ ) and 25% of sunlight (450 $\mu$㏖ m$^{-2}$ s$^{-1}$ ). Planting density in four light intensities was 30 cm$\times$30 cm. In each treatment, 100 trees were planted and height, diameter at root collar, biomass and chlorophyll contents were measured. The highest height, diameter at root collar and biomass in Abies holophylla seedlings were observed at 100% full sunlight. Lowest chlorophyll contents (Chlorophyll a, b and total) were shown at 25% of sunlight, lowest light intensity treatment in this study. Growths and chlorophyll contents of Abies holophylla seedlings were strongly associated with light intensity. Photosynthesis has to be investigated in near future because photosynthesis and chlorophyll contents were strongly related to tree growth with long periods.

• Journal of Korean Society of Forest Science
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• v.106 no.1
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• pp.33-39
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• 2017

This study was carried out in order to closely examine the growth and the physiological response by shading levels of a container seedling of Daphniphyllum macropodum which is known as the species of having shade tolerance in a warm temperature evergreen broad-leaved tree. The shading treatment was regulated with the shading level of full sunlight, and 35%, 55%, 75% of full sunlight. As a result of surveying the height and the root collar diameter growth by the shading level, the height was the highest with 13.2 cm under 35% of shading. The root collar diameter was the highest with 4.99 mm under full sunlight. The organ-based (leaves, shoot and root) dry mass production and the whole dry mass production were higher with 1.38 g, 0.47 g, 0.87 g, 2.72 g, respectively, in order of 35% shading. As for chlorophyll a, b, and the total chlorophyll content, the highest chlorophyll content was shown under 75% of shading with the relatively highest shading level. The ratio of chlorophyll a to chlorophyll b (a/b) was the highest under 35% of shading. The photosynthetic rate, stomatal conductance and water use efficiency were the highest with $12.3{\mu}molCO_2{\cdot}m^{-2}{\cdot}s^{-1}$, $0.27molH_2O{\cdot}m^{-2}{\cdot}s^{-1}$, $3.53{\mu}molCO_2{\cdot}mmol^{-1}H_2O$, respectively, under 35% of shading. As a result of surveying the whole experiment, D. macropodum grows nicely by maintaining 35% shading.

• Korean Journal of Agricultural and Forest Meteorology
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• v.2 no.4
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• pp.198-203
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• 2000

This study was conducted to investigate seasonal changes on height, root collar diameter, shoot growth, biomass and chlorophyll contents of Pinus strobus seedlings (two-year old) grown under different light environments in Sangju National University Nursery. Four shade treatments provided seedlings with full sunlight (1800 umo1 m$^{-2}$ s$^{-1}$ ), 75% of full sunlight (1350 umo1 m$^{-2}$ s$^{-1}$ ), 50% of full sunlight (900 ,umo1 m$^{-2}$ s$^{-1}$ ) and 25% of full sunlight (450 umo1 m$^{-2}$ s$^{-1}$ ). Planting density in four light intensities was 30 cm$\times$30cm. In each treatment, 10 trees were planted and height, diameter at root collar, shoot growth, biomass and chlorophyll contents (chlorophyll a, band a+ b) were measured. The highest height, root collar diameter, shoot growth and biomass in Pinus strobus seedlings were observed at full sunlight. Specially, root biomass was the most sensitive tree components part and strongly associated with decreases in the total biomass under low light intensity. The lowest chlorophyll contents (Chlorophyll a, band a+ b) were shown at 25% of sunlight, the lowest light intensity in this study.

• Journal of Korean Society of Forest Science
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• v.108 no.2
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• pp.189-199
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• 2019

The forest management paradigm has recently shifted from focusing on commercial production to focusing on ecosystem management. Accordingly, a natural seedling regeneration method that has a naturally high affinity has attracted much attention in recent years. The aim of this study was to determine the relationship among various environmental factors, lower stratum vegetation, and seedling regeneration in Pinus densiflora forests. The survey site comprised 50 sectors divided using the line transect method, and the survey data were divided into those from wet habitat (19 sites) and dry habitat (31 sites), depending on the soil humidity, and were analyzed separately to show the close relationship between soil humidity and natural seedling regeneration. As a result, the dry habitat exhibited high seedling density (157,419 trees/ha), with the main species being Quercus serrata, Zanthoxylum piperitum, Smilax china, and Pueraria lobata, while wet habitat exhibited low seedling density (57,895 trees/ha), with the main species being Stephanandra incisa, Castanea crenata, Lespedeza maximowiczii, Lysimachia barystachys, Aralia elata, and Styrax japonicus. The height and root-collar diameter under wet conditions exhibited faster growth than those under dry conditions. Height growth by the root-collar diameter in dry habitat increased faster than that in wet habitat. It was also confirmed that seedling regeneration in wet habitat exhibited a rapid growth pattern 5 years after germination. These results suggest that the seedlings begin to grow more rapidly after a period of suppression by competition with surrounding plants. Considering an ecosystem or ecological management approach, specific practices, such as bush control and vine clearing in wet habitats, should be more intensively conducted, especially at the beginning of the management operations.

• Journal of Korean Society of Forest Science
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• v.110 no.2
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• pp.189-197
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• 2021

This study aims to optimize the appropriate concentration of fertilizers for Sageretia thea by analyzing growth performances (height and root collar diameter) and physiological characteristics (photosynthesis, chlorophyll contents, and chlorophyll fluorescence reaction). As fertilizer concentration was increased to 1.5 g·L^-1, growth increased, but it decreased at 2.0 g·L^-1 treatment. Root collar diameter growth was reduced because of higher fertilizer concentrations. Photosynthesis reactions showed the highest CO₂ reaction curves, maximum photosynthesis rate, and maximum carboxylation rate in the 1.5 g·L^-1 fertilizer treatment. The chlorophyll fluorescence reaction and SPAD values revealed that fertilizer treatment improves photosynthesis efficiency and robustness compared with untreated control. Therefore, the appropriate fertilizer concentration for producing good seedling quality of Sageretia thea is 1.0~1.5 g·L^-1.

• Journal of Korean Society of Forest Science
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• v.111 no.4
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• pp.482-489
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• 2022

This study aimed to determine the optimal planting density of Fraxinus rhynchophylla assessed from the early growth performance at various planting densities over the 7-year period after planting. The study site was in Pyeongchang County, South Korea, and seedlings of 2-year-old (bare-root seedlings) F. rhynchophylla were planted at four densities (3,000, 5,000, 7,000, and 10,000 trees ha^-1) in March 2015. The survival rate, root-collar diameter (RCD), and height (H) were measured from 2015 to 2021, and the H/D (H/RCD) ratio and stem volume were calculated. The survival rate (84-97%) and H/D ratio (54.5-59.2%) were not affected by the planting density during the study period, but the RCD, H, and stem volume were significantly higher for 7,000 trees ha^-1 than for other planting densities. Especially, the stem volume (cm³ tree^-1) at 7 years after planting was highest for 7,000 trees ha^-1 (1,356.1), followed by 10,000 trees ha^-1 (958.6), 5,000 trees ha^-1 (773.0), and 3,000 trees ha^-1 (579.5). As the planting density increased, F. rhynchophylla seedlings showed initial rapid growth due to light competition, but relatively low growth at excessive planting densities. In the future, use of a suitable planting density considering planting costs should provide outstanding growth performance of F. rhynchophylla on plantations.