• Journal of Dairy Science and Biotechnology
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• v.14 no.2
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• pp.229-245
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• 1996

1. CM-Sephadex C-50-120 column을 사용하여 HLF를 효과적으로 정제하였다. CM-Sepha-dex 50-120 column chromatography를 수행한 결과, HLF는 NaCl 350 mM-500 mM 사이에서 용출되었으며, 용출된 분획을 SDS-PAGE를 수행해서 단일 band를 확인하였다. Anti-HLF antibody를 이용한 Western blotting 결과 nitrocellulose paper 상에 단일 band 가 나타나므로 HLF 가 효과적으로 정제되었다는 것을 알 수 있었다. 2. Con A, PWM, PWA LPS 등의 자극원으로 단핵구와 대식세포를 자극한 다음 BLF를처리한 배 양액을 IL-1 bioassy한 결과는 Con A 33%, PMA 33%, PWM 15%, LPS 35% 이고, HLF로 처리하여 IL-1 bioassay를 한 결과는 Con A 15%, PMA 22%, PWM 10%, LPS 5%로 감소된 것으로 나타났다. 3. K-562 세포를 이용한 colony forming assay에서 BLF가 10 g/ml일 때는 30%, 30 g/ml일 때는 35%, HLF는 10 g/ml일 때는 5%,30 g/ml일 때는 30%의 저해를 나타냈다. 4. Lactoferrin의 면역증강효과를 알아보기 위하여 hapten인 VCR-BSA를 투여 한 후, 생성되는 항체생성능력을 ELISA로 비교하였다. 그 결과 HLF 및 BLF 투여군에서 대조군에 비하여 adjuvant 효과를 관찰할 수 있었다. 또한 이때 macrophage 수는 대조군에 비하여 HLF와 BLF를 투여한 군이 증가됨을 알 수 있었다. 이러한 결과로 미루어 볼 때 LF는 macrophage를 활성화 시켜서 항체 생성 능력을 증가시키는 효과가 있음을 관찰할 수 있었다. 5. Balb/c mouse의 thymus로부터 분리한 CD4- CD8- 세포를 BLF로 처리하여 24 시간 배양한 후 CB4$^-$ CD8$^-$ 세포의 분화를 측정한 결과, CD4$^-$를 CD4$^+$ 로 분화하였다. 그리고 HLF로 처리하여 24 시간 배양 후 CD4$^-$ CD8$^-$ 세포의 분화를 측정한 결과, CD4$^-$ CD8$^-$를 CD4$^+$ CD8$^+$ 로 분화하였다. 6. Lactoferrin이 T cell의 IL-2 production에 미치는 영향은 PMA 처리군, PMA+OKT3처리군, LF 단독 처리군 보다 PMA+OKT3+LF를 처리한 군이 IL-2 생성에 영향을 미쳤다. 7. Lfcin B의 단일크론항체에 의해 인식되어지는 Lfcin B의 항원결정기는 ‘QWR’로 밝혀졌다.

• Korean Journal of Food Science and Technology
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• v.34 no.4
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• pp.642-646
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• 2002

Pysicochemical and sensory characteristics of cookies with various levels of functional rice flour were investigated in this study. Dough pH of 10% substituted group and Spreadability of 20 and 30% substituted group had significantly higher values than that of control group. Density of Dough had significantly the largest value of 1.27 with 10% substituted sample group. The L value of brightness in control group had significantly the largest value of 69.31. The b value of 20% substituted sample group had significantly the largest value of 35.60. Results of sensory characteristics showed significantly higher savory aroma, savory flavor, hardness and brownness with 10% substituted sample group. Acceptance test of cookies with 20% substituted sample group showed higher overall acceptability than those of others. Quality caracteristics of 10 to 30% subsituted sample groups indicated significantly similar or higher values compared to those of control showing the possibilities of developments in health concerned funtional rice coopkies.

• Journal of the Korea Convergence Society
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• v.12 no.8
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• pp.77-83
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• 2021

The purpose of this study was to determine whether the convergence pulmonary function of the 20s men of mild intellectual disabilities with obesity to tredmill exercise. Ten subjects of mild intellectual disabilities with obesity(experimentals) and ten contrary subjects(controls) were participated in the experiment. experimental group conducted treadmill gait training and control group conducted automed exercise. Subjects were assessed for their respiratory function by using Fit mate. The result of the experiments showed high pulmonary function than the controls. Thus, it suggests that the respiratory functional data of 20s men of mild intellectual disabilities with obesity can be used as a various respiratory one for the exercise programs in the area.

• Journal of the Korean Society of Food Science and Nutrition
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• v.22 no.4
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• pp.423-427
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• 1993

Various kinds of jeolpyon (cubed rice cake) differing in soybean content were prepared. By the sensory and instrumental analysis, it seemed that addition of soybean powder for jeolpyon should be less than 5%. Jeolpyon with 5% soybean powder was not significantly different from the control.

• Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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• 2005.04a
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• pp.90-93
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• 2005

경상남도 합천군과 창녕군 생활용수 지하수 관정 개발시 지하수 굴착공종으로부터 얻어진 구간별 지층자료와 대수층 수질 자료를 획득하여 이용하는 방법을 연구하였다. 지층정보를 파악하기 위하여 지하수공 굴착심도 10m마다 슬라임을 채취하였으며, 대수층 관통시에 지하수 시료를 구간별로 채수하여 전기전도도 및 수질시료를 분석하였다. 경도가 높은 지역의 경우, 음용수 수질기준치에 적합한 지하수를 개발하기 위해서 대수층별 전기전도도를 분석측정하여 경도를 간접적으로 파악함으로써 지하수 굴착심도 결정에 이용하였다. 그리고 대수층 구간별 불소 농도를 파악하였으며, 불소 농도가 높은 대수층 구간에서는 하부대수층 메움 등을 실시하였다. 대수층의 수질검사결과에서 기준치를 초과하는 우려 인자들(불소, 경도, 증발잔류물, 질산성 질소, 철, 알루미늄, 아연 등)에 대한 수질기준 초과 가능성을 대비한 양수시험 모니터링을 설계함으로써 지하수공의 수질 특성 파악이 가능하였다. 양수시험과정에서 탁도가 시간에 따라 기준치 이하까지 감소하는 일반적인 경향과 기준치 이상에서 더 이상 감소하지 않는 경향에 대한 원인을 분석할 수 있었다.

• The Journal of Korean Orthopaedic Ultrasound Society
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• v.2 no.1
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• pp.7-12
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• 2009

Purpose: The purpose of this study was to correlate the degree of sonographic medial meniscal extrusion with MRI and arthroscopic findings. Materials and Methods: Out of these 32 patients, with medial meniscal tear who went on arthroscopic surgery, 14 patients had the medial meniscal tear and 18 patients had both lateral and medial meniscal tear. The extent of meniscal extrusion without meniscal tear was assesed in 24 patients who did not show meniscal tear on MRI. The extent of meniscal extrusion was measured between tibial medial joint line (excluded osteophyte) and the outer margin of the medial meniscus. Kellgren-Lawrence grading scale was assessed in plain X-ray image and the location of lesion was assessed during arthroscopic surgery. We also measured the extent of meniscal extrusion on MRI and sonography respectively, and compared each other. Results: There was significant difference between patients with medial meniscal tear and both lateral and medial meniscal tear, patients with medial meniscal tear, patients with both medial and lateral meniscal tear, patients without meniscal tear (P value<0.05). Conclusion: The Meniscus tear must be considered when sonography shows the meniscal extrusion more than 5 mm in length.

• Korean journal of food and cookery science
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• v.17 no.2
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• pp.156-164
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• 2001

Four different oligosaccharides used to determine their effects on gelatinization and retrogradation of Backsulgies(BSG), Korean traditional rice cake, were cyclodextrin, isomaltooligosaccharide, fructooligosaccharide and maltotetrose, with 3 or 6%(w/w), based on the rice flour. From the Amylograph and blue value data, adding 6% cyclodextrin into the rice flour fastened the gelatinzation process, and delayed the retrogradation in stored BSG at 4$\^{C}$ for up to 3 days, probably due to its cyclic structure. Using rheometer, the hardness of freshly made BSG added with cyclodextrin was significantly lower than that of control BSG, and increased with storage time in all BSGs at refrigerated temperature. When using maltotetrose in rice flour, the hardness of BSG was also significantly low, but slightly higher than that of cyclodextrin. Hunter “L” value in BSG decreased with the addition of oligosaccharides, but the higher the level of oligosaccharide in BSG, the insignificant the Hunter “a”,“b” values of BSG. From the X-ray diffraction studies, the rice flour showed typical A pattern, and the crystallinities of all BSG gave amorphous V type. The highest peaks of X-ray patterns in BSG added with 6% cyclodextrin or maltotetrose were, however, lower than that of the control, meaning the less crystalline, retrograded starch in the former BSGs. Based on sensory evaluation, BSG added with all oligosacchrides were not significantly different in taste, flavor, chewiness, moistness and overall preferences, with slightly darker color with longer storage time, compared to the control BSG.

• 한국노년학
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• v.32 no.3
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• pp.747-758
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• 2012

Detecting probable Alzheimer's disease (AD) at an early stage is crucial in slowing the progression of the disease and initiating drug therapy for more effective symptom management. Therefore, this study aimed to identify linguistic features that allow us to distinguish between patients with AD and normal controls. This paper reports on characteristics of spontaneous speech in subjects in three stages of AD (questionable, mild, moderate) compared with education- and age-matched normal controls. Four components of speech were measured in Korean native speakers with AD and normal aging: speech tempo, hesitation (measured in seconds), rate of articulation errors, and rate of grammatical errors. The results revealed significant differences in most of these speech components among the four groups, including significant differences between normal controls and the questionable AD group in the areas of speech tempo and rate of grammatical errors. Phonological? articulatory ability was preserved in questionable AD, and grammatical ability was preserved in questionable and mild AD. Subjects with moderate AD were severely impaired in grammatical ability. Prospective assessments of spontaneous speech skills using a dialogue and picture-description task are useful in detecting the subtle, spontaneous speech impairments that AD causes even in its early stage.

• Journal of dental hygiene science
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• v.13 no.3
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• pp.230-237
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• 2013

In this research, the degree of depression and death orientation of the 332 female students majoring in dental hygiene at colleges located in Gyeongsangbuk-do Province were examined and analyzed through a structured self-administered questionnaire from May to July, 2012, for the purpose of improving their mental health. The results are as follows: 1. The degree of depression of the subject students majoring in dental hygiene was shown that 59.9%, 36.8%, 3.0%, and 0.3% of the students were in the normal group, mild depression group, moderate level of depression group, and severe depression group, respectively. 2. The grand mean and the standard deviation of the subjects'death orientation was $2.09{\pm}0.62$ points. 3. The degree of depression of the subjects whose family's economic level was low, whose satisfaction with their family background was low, whose satisfaction level with their major was low, whose school records was low, who frequently thought about death, and who attempted suicide was high in a meaningful way, and death orientation was meaningfully low. In addition, those in the group with the experience of feeling suicidal were proved to have a significantly high degree of depression, and those without the experience of education about death had a low level of death orientation. 4. The degree of depression and death orientation had a positive correlation. When the study results above are combined, follow-up research needs to be conducted so that differentiated programs linked with the care for mental health for the formation of proper attitudes toward depression and death will be established to help students lead a positive life.

• Korean Journal of Food Science and Technology
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• v.41 no.1
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• pp.69-76
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• 2009

This study was conducted to evaluate the physicochemical and sensory characteristics of chiffon cakes made with rice flour ranging from 20-100% in place of wheat flour. The water binding capacity of the rice flour was greater than that of the wheat flour and its fat binding capacity was lower than that of the wheat flour. The batter stability data indicated that the 100% treatment was better than the control, and specific gravity values and specific loaf volumes were lower in the samples with added rice flour. Texture analysis found no significant differences in hardness, adhesiveness, cohesiveness, gumminess, and chewiness between the control and the 100% treatment. In the case of crust color, the rice flour-added chiffon cakes did not show any significant differences for their 'a' values. In addition, their crumb color was not significantly different in terms of both their 'L' and 'a' values. According to the sensory evaluation, there was no significant difference in overall acceptability between the control and the rice flour-added chiffon cakes. The highest sensory scores were obtained by the 100% treatment for overall acceptance and moistness. However, flavor, cohesiveness, and brittleness were not significantly different. These study results show that wheat flour could be replaced by rice flour up to 100% in chiffon cake.