• Journal of the Korean Society of Food Science and Nutrition
• /
• v.36 no.11
• /
• pp.1385-1390
• /
• 2007

Anticarcinogenic-active compound was isolated and purified from Angelica gigas Nakai. The compound was identified as decursin ($C_{19}H_{20}O_5$; molecular weight 328) by mass, IR spectrophotometry $^1H-NMR$ and $^{13}C-NMR$. The proliferation decreased in a dose dependant fashion in the MCF-7 cells treated with decursin for 24 hours over the concentration of $20{\mu}g/mL$. The $IC_{50}$ value of the decursin treatment for 24 hours were 31.04, 33.60, 27,24, $20.45{\mu}g/mL$ in the SW480, 293, HepG2 and MCF-7 cells, respectively, The growth inhibitory effect was stronger in the MCF-7 cells compared to other cells including 293 of human normal cells. The chromatin condensation, apoptotic body formation and DNA fragmentation were examined in the cells treated with decursin. These results suggest that decursin from Angelica gigas Nakai inhibited the growth through apoptosis in MCF-7 cells.

• Applied Microscopy
• /
• v.17 no.2
• /
• pp.1-22
• /
• 1987

Pemphigus vulgar교의 본태(本態)를 알아보기 위한 일환으로 본질병(本疾病)의 병변조직(病變組織)을 전자현미경적(電子顯微鏡的)으로 관찰(觀察)하고 본질병(本疾病)에 관여(關與)하는 세포(細胞)들에 대(對)해 면역전자현미경적(免疫電子顯微鏡的)으로 추구(追究)하였던 바, 그 결과(結果)를 보고(報告)하는 바이다. 견(犬)의 pemphigus vulgaris의 구강(口腔) 및 식도(食道)의 점막층(粘膜層)을 전자현미경적(電子顯微鏡的)으로 관찰(觀察)하였던 바 acantholysis를 일으켜도 desmosome과 기저막(基底膜)은 큰 변화(變化)가 없었으며 세포간(細胞間)은 세포간물질(細胞間物質)의 집적(集積)에 의(依)해 확장(擴張)되고 이들 세포간강물질(細胞間腔物質)은 집괴(集塊) 또는 무구조(無構造)한 물질(物質)로 나타났다. 그리고 기저세포(基底細胞)는 기저막(基底膜)에 단단히 부착(附着)되어 있었고 dendritic cell이 기저세포층(基底細胞層)위로 분포(分布)되어 있었으며, 이들 dendritic cell 중(中)에서는 가끔 여러 형태(形態)의 퇴행성변화(退行性變化)를 볼 수 있었다. Mouse 피부유리상피세포(皮膚遊離上皮細胞)에 있어서 immunogold-labeling 방법(方法)에 의해 dendritic cell을 동정(同定)하는 데에는 post-fixation, pre-embedding immunogold-labeling technique가 좋았으며 15nm와 40nm 크기의 colloid-fold 입자(粒子)로 Langerhans cell과 Thy-1양성(陽性) dendritic cell이 표식(標識)될 수 있었다. 이들 세포(細胞)들은 세포막항원(細胞膜抗元)에 따라 monoclonal antibody의 반응(反應)에 이어 치밀한 colloid-gold 입자(粒子)가 세포막표면(細胞膜表面)을 따라 일정(一定)하게 표식(標識)되었다. 또한 이들 상피세포(上皮細胞)들을 투과전자현미경적(透過電子顯微鏡的)으로 관찰(觀察)하였을 때 초미세구조(超微細構造)가 잘 보존(保存)되었으나 Langer-hans cell내(內)의 Birbeck granule은 유리전(遊離前) 피부상피조직내(皮膚上皮組織內)의 Langerhans cell내(內)의 Birbeck granule에 비(比)해 수적(數的)으로 현저히 감소(減少)되어 있었다. 그러나 Thy-1 양성(陽性) dendritic cell에서 볼 수 있는 dense-core 과립(顆粒)은 별변화(別變化)없이 쉽게 관찰(觀察)될 수 있었다. 조직배양(組織培養)을 한 견(犬)의 keratinocyte에 대(對)해 사람 pemphigus vulgaris의 항체(抗體)로 반응(反應)시킨 후 protein-A gold(15 nm)로 표식(標識)시킨 바 제일 바깥 상층(上層)의 keratinocyte에 있어서 세포막표면(細胞膜表面)을 따라 표식(標識)되어 세포막항원(細胞膜抗元)을 나타내었으며, 이와 같은 소견(所見)으로 미루어 정상피부(正常皮膚) 중층편평상피세포(重層扁平上皮細胞)에서도 동일(同一)한 소견(所見)을 관찰(觀察)할 수 있다고 본다.

• Journal of Life Science
• /
• v.27 no.1
• /
• pp.1-7
• /
• 2017

Abnormal actin remodeling is a typical characteristic of tumor cells. Thymosin ${\beta}_{10}$ (TB10) and profilin-1 (PFN-1) are actin-binding proteins and essential regulators of actin polymerization. We previously showed that TB10 induced death in ovarian cancer cells by sequestering F-actin, but the underlying mechanisms of this induction have not been explored. In this study, we identified TB10 as a novel regulator of PFN-1 and demonstrated its novel function as a tumor suppressor in ovarian cancer cell lines. The present study investigated protein expression profiles through polyacrylamide gel electrophoresis (PAGE) and liquid chromatography-mass spectroscopy (LC-MS/MS) in SKOV3 cells, an ovarian cancer cell line, that were transiently transfected with TB10. PFN-1 was highly overexpressed in response to TB10, and overexpression of PFN-1 resulted in inhibition of cell proliferation and migration and promotion of cellular apoptosis in ovarian cancer cells. Furthermore, transiently transfected PFN-1 appeared to deactivate the Erk signaling pathway, followed by decreased expression of Elk-1 and Egr-1 in human ovarian cancer cells. Interestingly, PFN-1 did not affect the activation of Akt. The results demonstrated that PFN-1 induced apoptotic cell death and inhibited proliferation and migration in ovarian cancer cells, suggesting that PFN-1 may be valuable in anti-cancer therapy.

• Journal of fish pathology
• /
• v.1 no.1
• /
• pp.31-37
• /
• 1988

An experiment was carried out in order to reveal the infection species of trematode and the infection demage in the marine bivalves. Metacercaria of Echinostomatidae were found in Mactra veneriformis, Cyclina sinensis and Solen strictus in the vicinity sea of Naecho-do, the estuary of the Keum River in the western coast of Korea. The metacercaria detected from Solen strictus were fed by Larus crassiostris, at 16 days after infection, the adult worm could be seceded. The metacercaria of Himasthla kusasigi were detected from Mactra veneriformis and Cyclina sinensis, their infection rates were 70.4% and 85.7% respectively. The partial infection rates with respect to the body parts were 40.4% and 77.3% in the foot, 17.0% and 12.4% in the gill, 12.6% and 10.3% in the mantle in order. The metacercaria of Acanthoparyphium were found from Solen strictus. The total infection rate was 63% and the partial infection rate was 63% and the partial infection rate was shown foot, mantle and gill in order. The adult worm ceded from Larus crassiostris was indentified as Acanthoparyphium tyosenensis Yamaguti, 1938 from view point of its physical characteristics. Therefore, Mactra veneriformis, Cyclina sinensis would be added as new intermediate hosts, and Echoinostoma decteded from Mactra sulcataria which was studied by author(1969) was revealed as larvae of Acanthoparyphium tyosenensis.

• Journal of fish pathology
• /
• v.24 no.3
• /
• pp.225-236
• /
• 2011

This study was aimed to determine the fish physical status according to the gross and histopathological findings of liver in cultured black rock fish, Sebastes schlegeli. All 47 fish submitted had no marked abnormalities in the external findings. 42.55% of fish showed normal liver, 25.53% yellow liver, 25.53% atrophic brown liver, 4.26% yellowish-green liver and 2.13% fatty liver in gross examination. Grossly normal liver showed no remarkable change in lobular structure but many vacuoles were found in hepatic cell. Hepatic cells took normal roundish, polygonal shapes containing spherical nuclei. In group of yellow-brown liver, many brown pigments were seen in hepatic cells, MMCs and brown-colored hyaline droplets within cytoplasm of hepatocytes. Yellowish green pigments were seen in hepatic cells and MMCs of yellow green liver and green colored hyaline droplets within hepatocytes. The dilated central veins are highlighted with atrophy of hepatic cells. Outline of atrophic hepatocyte became ambiguous and nucleus frequently become small and pyknotic. Fatty liver showed prominent vacuolar structures in cells as clear spaces or foamy cytoplasm with degenerative nuclei. From these results, it was strongly suggested that hepatic gross and histological findings could be used as important and critical health parameters of fish prior to progression to substantial manifestation as clinical disease.

• The Korean Journal of Food And Nutrition
• /
• v.17 no.2
• /
• pp.156-162
• /
• 2004

This study was performed as a part of comparative biological studies of carotenoid pigment for the unutilized biological products. The content of total carotenoid in the integument of wild catfish were 0.27(${\pm}$0.7) mg％ and composed of 25.8％ zeaxanthin, 9.7％ diatoxanthin and 9.1 ％ cynthiaxanthin as major carotenoid. The content of total carotenoid in the integument of cultured catfish were 0.07(${\pm}$0.03) mg％ which is relatively lower compare to wild catfish and composed of 48.5％ lutein, 13.9％ zeaxanthin and 13.3％ isocryptoxanthin as major carotenoid. The total carotenoid contents of the slender catfish were 0.75(${\pm}$0.25) mg％ which is relatively higher compare to other species of catfishes. The carotenoids were composed of 24.5％ zeaxanthin, 24.1 ％ 7'8'-dihydro-${\beta}$-carotene-4-ol, 17.9％ 7'8'-dihydro-${\beta}$-carotene and 10.8％ 7'8'-dihydro-${\beta}$-carotene-3-01 as major carotenoid and 8.7％ diatoxanthin, 6.7％ cynthiaxanthin and 5.0％ lutein as minor carotenoid. Based on these data, as a comparative studies of carotenoid in integument of siluridae, parasiloxanthin and 7',8'-dihydroparasiloxanthin which are the characteristic carotenoid of catfish from biwa lake in Japan, Slender catfish contained more based on 7',8'-dihydro-${\beta}$-carotene while that of wild and cultured catfishes were not found, indicating that carotenoid pigment of slender catfish depend on their living conditions.

• Proceedings of the KOR-KST Conference
• /
• 1993.07a
• /
• pp.235-247
• /
• 1993

이십일세기상해항공운수발전전략연구, 시이 $\ll$진흥상해, 개발포동, 복무전국, 면향세계$\gg$ 적방침작위지도, 병통과이상해포동개방개\ulcorner\ulcorner\ulcorner장강삼각주지구내지정개장강류역경제발전, 축보사상해발전성위\ulcorner\ulcorner지 구경제, 김융, 무역중심지일작위목표개전전략연구. 상해재아국두\ulcorner\ulcorner의현대화건설진정중점유겁기중요적지위, 저장성위태평양서안최인적 제무역중심지일, 목전, 상해항공운수적현상여기재국민경제중적전략지 위급여세계경제발달국가급지구례여뉴약, 륜돈, 파려, 지가가, 동경, 향항, 신가파등국제대성시상비차거태대, 안통계수거상해1990년항공운수탄토량약시미국뉴약1986년적1.5%, 시향항적10%좌우, 가견상해민\ulcorner\ulcorner수적 발전속도이경한제료상해경제적등비, 한제료포동개방개발급여국내 외정치경제화문화적교류, 위료사상해항공운수여상해적국민경제급사사회 발전상괄응, 대력발전항공운수기박재미첩. 수착상해경제발전, 능원, 원재료적증가, 외향형경제발전급금후궤십년상해인민수입증가화경제결 구조정.고정첨기출적발전급국제교왕적증가. 예측이십일세기상해여객 운수량급화운량장성배증가, 소이탐색일개여상해경제발전전략목표상괄 응적현대화항공교통운수체계, 발혼항공운수특유적우핵시십분필요적. 본연구보고종이하오개방면대상해이십일세기항공운수발전전략진행천술. 일, 개술발전상해항공운수적필요성급긴박성. 이, 상해민용항공운수현상대상해지구주요승운인동방항공공사항선 망, 운력, 기대, 기장현상작료분석,설명상해민항유우운력불족, 기출 장비체후, 자김결, 불능만족상해경제등비발전적수요. 공중교통관제, 통신도항, 기무유수, 항유공응등방면야도불동정도지한 제료항공운수적발전, 필수인기겁대적중시. 삼, 상해항공운수적예측화전망, 통과자료연구급수거분석, 리용수 학모형대이십일세기상해항공운량진행예측, 제출발전상해항공운수적전 략목표급발전중점. 예측2020년 상해항공항총객운탄토량4300만인/년, 화운량달120만돈; 2050년객운량장달18150만인차/년, 화운량518만돈. 사, 발전상해민항기출경제정책, 제출위료$\ll$진흥상해, 개발포동, 복무전국, 면향세계$\gg$ 화도이십일세기중기국민경제달도중등발달국가수 평굉관전략목표적실현, 제료필수재지도사상상파교통운수진정방도전략 산업지위, 환응재관리체제상채취과단유효적개혁조시, 재기출경제정책 상급여대력부지. 오, 전략목표, 위파상해건설성위태평양서안최대적경제, 김융, 무역적중심, 요구상해항공항성위화동지구통향세계각지항선망출발참, 구성대외개방선면축심, 실현국제항선적함접화국내항반적전항, 형성다축심복사식항선망; 가강기장건설, 개피포동제이국제기장건설, 괄응포동개발경제발전적수요.

• Korean Journal of Microbiology
• /
• v.41 no.3
• /
• pp.208-215
• /
• 2005

This study was accomplished in order to collect fundamental data on microbial roles in recycling process of reed rhizosphere. Sunchon bay, which is considered as one of the marsh and mud environments severely affected by human activities such agriculture and fisheries, was selected as a model place. In our initial efforts, two bacterial consortia were obtained by enrichment culture using PAH mixtures containing anthracene, naphthalene, phenanthrene and pyrene as the sources of carbon and energy, and four pure bacteria capable of rapid degradation of PAH were isolated from them. Four strains designated as SCB1, SCB2, SCB6, and SCB7 revealed by morphological, physiological and molecular analyses were identified as Burkholderia anthina, Alcaligenes sp., Achromobacter xylosoxidans., and Pseudomonas putida, respectively with over $99{\%}$ confidence. Notably, Burkholderia anthina SCB1 and Alcaligenes sp. SCB2 were found to utilize anthracene and pyrene more quickly than naphthalene and phenanthrene, whereas Achromobacter xylosoxidans SCB6 and Pseudomonas putida SCB7 exhibited similar growth and degradation patterns except for pyrene. These facts suggest that the rhizosphere microorganisms capable of PAH degradation might be used to clean up the contamination sites with polycyclic aromatic hydrocarbons.

• Korean Journal of Microbiology
• /
• v.49 no.1
• /
• pp.90-94
• /
• 2013

A new Bacillus strain designated as MP56 producing antimicrobial substance has been isolated from the mud flat of Korea. The strain MP56 was found to exhibit broad spectrum of antimicrobial activity against Gram-positive pathogenic microorganisms and MDR (multi drug resistant) strains. The 16S rRNA sequence revealed that the MP56 was closely related to Bacillus subtilis with 99.93% homology. The optimal medium composition for production of antimicrobial substance in the B. subtilis MP56 were 1% mannitol, 1% oat meal, 0.01% $CaCl_2$. Antimicrobial activity of the culture broth against different pathogenic strains was assessed using the antimicrobial spectrum. The result suggests that Bacillus strain MP56 produces high quality antimicrobial substance that might be very useful to control varieties of pathogenic microbial growth.

• Korean Journal of Food Science and Technology
• /
• v.43 no.2
• /
• pp.149-155
• /
• 2011

This study was carried out to develop a method of removing unnecessary sugars and high-molecular weight soluble components from water extract by using various highly porous polymer resins: these resins are widely used as adsorbents for polyphenolic compounds. Each anthocyanin-rich fraction (ARF) that was eluted from column packed with 4 different resins (SEPABEADS SP207, 700, 850 and Diaion HP 20) was obtained from an aqueous extract of the ripe fruits of Rubus coreanus, which is well-known in Korea as "Bok-bun-ja". Among the above mentioned resins, Diaion HP20 had the highest efficacy and provided maximum yield of the ARF. The ARF eluted from the column packed with HP 20 exhibited the strongest antioxidant activity in vitro. Bioactive phytochemicals extracted from Rubus coreanus were investigated using the on-line HPLC-$ABTS^{{\cdot}+}$ system, and the resulting 6 peaks were identified as radical-scavenging components. By using liquid chromatography-mass spectrometry, 5 peaks were obtained, and these were identified as cyanidin-3-sambubioside, cyanidin-3-glucoside, cyanidin-3-xylosylrutinoside, cyanidin-3-rutinoside and pelargonidin-3-rutinoside. These results indicate that the use of appropriate porous resin (Diaion HP 20) leads to an increase in the yield of bioactive components and enhancement of their biological properties.