• Korean Chemical Engineering Research
• /
• v.55 no.3
• /
• pp.369-378
• /
• 2017

Vascular endotherial growth factor (VEGF) is a potent mitogen that stimulates vascular permeability and angiogenesis and has a potential in therapeutic applications. An industrial production method that provides high yield as well as purity is needed. Researches for various factors of mild solubilization with combination of ubiquitin fusion protein to increase solubility were carried out as well as by changing pH and denaturant concentration. Usage of pET28-a bacteral expression vector in BL21 (DE3) host cell was capable of producing approximately 14 g/L VEGF fusion protein in 20L fermentor. A purification process consisting of four chromatography steps including refolding and digestion with UBP1 resulted in mild solublization under the conditions of 2M urea and pH 10.0 due to ubiquitin fusion tag protein that increases in solubility of target protein VEGF. High yield of refolding and dimerization could be obtained between two step Ni-affinity chromatography. Multimeric and misfolded proteins and endotoxin were removed by DEAE anion exchange chromatography. Final monomers were removed from dimers by gel filtration chromatography. Characterization analysis of purified dimeric VEGF was performed using SDS-PAGE and RP-HPLC with a purity of 97%.

• The korean journal of orthodontics
• /
• v.39 no.6
• /
• pp.383-392
• /
• 2009

Objective: The purpose of this study was to evaluate the efficacy of a slim bristled toothbrush compared with a V-shaped orthodontic toothbrush in patients with fixed orthodontic appliances. Methods: Thirty four orthodontic patients receiving edgewise treatment were randomly assigned to two groups, a slim bristled toothbrush (Nano silver slim care) and a V-shaped toothbrush (Oral-B). Plaque index, gingival index and bleeding index were recorded at the beginning of the study (baseline), 2 weeks, 4 weeks and 6 weeks after new toothbrushes were supplied and the results were analyzed statistically. Results: No statistically significant differences were found for plaque index, gingival index and bleeding index between toothbrush groups during the 6 weeks. Plaque and gingival indices were decreased at 2 weeks and 4 weeks but increased at 6 weeks in both toothbrush groups. Bleeding index in the V-shaped toothbrush group showed the lowest value at 2 weeks then increased at 4 weeks and 6 weeks but in the slim bristled toothbrush group decreased from 2 weeks through 6 weeks to under baseline levels. Patients in their twenties had significantly lower values than teenagers in the slim bristled toothbrush group (p < 0.013). Conclusions: The results would suggest that both of the toothbrushes are equally effective but the use of a slim bristled toothbrush may be of benefit in promoting gingival health for fixed orthodontic appliance patients in their twenties and over.

• Development and Reproduction
• /
• v.5 no.1
• /
• pp.59-71
• /
• 2001

Since the first radioimmunoassay (RIA) was developed in 1970s, many conventional RIAs and non-isotopic immunoassays (NIA) had been developed in which the degree of precision, accuracy, specificity and practicability have progressively increased. Recently ultrasensitive assay method at femtogram to determine testosterone in serum, saliva and feces is required for the study of sexual dysfunctions in male and female, monitoring the psychological stress and conditions, aging process such as menopause and partial androgen deficiency in aging male, the hormonal changes of small experimental animals etc. This review discussed the recentd evelopments of steroid assay methods, based upon the testosterone assay results of authors far 20 years, and the problems associated the assay set-up, the characterizations and applications of the established procedures, and desifls of assay, reliablity criteria, and the practical aspects of assay set-up and application, based upon the data of the authors. The present study demonstrates the general problems methods to be consider in order to set up the highly sensitive assay methods and to increase the assay quality and the necessity of assay quality control program. To improve the assay quality of each laboratory and to compare the assay results in homeland, the national QC programs should be organized.

• Journal of the Korean Institute of Gas
• /
• v.17 no.3
• /
• pp.8-13
• /
• 2013

The purpose of this paper is to study for fire example by fuel leakage in LPG Vehicle. At first example, the car was repaired the fuel line that was connected with pressure hose between fuel regulator and injector in engine. But the service man was not very tighten with regular torque. At a result, the gas leaked on hot parts of engine. It verified the production of fire by engine heat. At second example, when the repair man, after replacement the injector, inserted the injector in a assembling part of it, he didn't the transform condition of fixing part. Therefore, the tearing phenomenon of O ring producted the controlled leakage of fuel by the injector deflection. It found the fact that the fuel leaked with gap of O ring. At third example. the fuel-cut solenoid valve was lined with pressure regulator unit. But the service man didn't throughly certify the leaked work of connected parts after repaired it. As a result, it certified the fire by engine heating leaked liquefied petroleum gas. Therefore it have to minimize the fire production that the driver should do no problem to throughly manage the fuel system.

• Journal of the Korean Magnetics Society
• /
• v.24 no.2
• /
• pp.35-40
• /
• 2014

We investigate magnetism and magnetocrystalline anisotropy of CoFe thin films, using VASP code in GGA. In this study Co-terminated and Fe-terminated 5-layer CoFe thin films are employed. The Co-terminated CoFe thin film shows two total energy minima at 2-dimensional lattice constants of $2.45{\AA}$ and $2.76{\AA}$. The film of $2.45{\AA}$ has fcc-like structure and the film of $2.76{\AA}$ has bcc-like structure similarly to a bulk CoFe alloy. And the fcc-like film is more stable by the energy difference of about 160 meV compared to the bcc-like film. The Fe-terminated CoFe film shows very complicated behaviour of total energy which is suspected to be closely related to its complex magnetic structure. The Co-terminated CoFe film of $2.76{\AA}$ shows perpendicular magnetocrystalline anisotropy (MCA), while the film of 2.45 does parallel MCA. The Fe-terminated CoFe film also exhibits similar MCA behaviour.

• Applied Chemistry for Engineering
• /
• v.30 no.4
• /
• pp.429-434
• /
• 2019

A lateral flow immunoassay platform utilizing antibody functionalized water soluble CdSe/ZnS semiconductor quantum dots (QDs) was developed for the analysis of human serum amyloid A-1 (hSAA1) in a buffer solution. hSAA1 was chosen as a target protein because it is regarded as a potential biomarker associated with early diagnosis and prognosis in patients of lung cancer. The immunoassay strip on a nitrocellulose membrane was fabricated by spraying two lines composed of a test line with a monoclonal antibody against hSAA1 (10G1) (anti hSAA1) and a control line of anti-chicken IgY. While the CdSe/ZnS QDs synthesized in an organic phase were transferred to a water phase by ligand exchange using carboxylic acid modified alkane thiol. The QDs was then conjugated to monoclonal antibody against hSAA1 (14F8) [anti hSAA1 (14F8)] and used as a fluorescent detection probe. The sequential lateral flow of hSAA1 in different concentration and QDs-anti hSAA1 (14F8) complex allowed to form the surface sandwich complex of anti hSAA1 (10G1)/hSAA1/QD-anti hSAA1 (14F8), which was then analyzed using fluorescence microscope. A 100 nM concentration of hSAA1 protein can be detected by naked eyes under an optimized lateral flow buffer condition with a sensing time of 5 mins.

• Journal of the Korea Institute of Information Security & Cryptology
• /
• v.12 no.2
• /
• pp.45-52
• /
• 2002

In this contributions, we propose a new MSB(most significant bit) algorithm based on AOP(All One Polynomial) and two parallel semi-systolic architectures to computes $AB^2$over finite field $GF(2^m)$. The proposed architectures are based on standard basis and use the property of irreducible AOP(All One Polynomial) which is all coefficients of 1. The proposed parallel semi-systolic architecture(PSM) has the critical path of $D_{AND2^+}D_{XOR2}$ per cell and the latency of m+1. The modified parallel semi-systolic architecture(WPSM) has the critical path of $D_{XOR2}$ per cell and has the same latency with PSM. The proposed two architectures, PSM and MPSM, have a low latency and a small hardware complexity compared to the previous architectures. They can be used as a basic architecture for exponentiation, division, and inversion. Since the proposed architectures have regularity, modularity and concurrency, they are suitable for VLSI implementation. They can be used as a basic architecture for algorithms, such as the Diffie-Hellman key exchange scheme, the Digital Signature Algorithm(DSA), and the ElGamal encryption scheme which are needed exponentiation operation. The application of the algorithms can be used cryptosystem implementation based on elliptic curve.

• Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
• /
• v.39 no.3
• /
• pp.187-201
• /
• 2021

Recently, the demand for a 3D urban spatial information infrastructure for storing, operating, and analyzing a large number of digital data produced in cities is increasing. CityGML is a 3D spatial information data standard of OGC (Open Geospatial Consortium), which has strengths in the exchange and attribute expression of city data. Cases of constructing 3D urban spatial data in CityGML format has emerged on several cities such as Singapore and New York. However, the current ecosystem for the creation and editing of CityGML data is limited in constructing CityGML data on a large scale because of lack of completeness compared to commercial programs used to construct 3D data such as sketchup or 3d max. Therefore, in this study, a method of constructing CityGML data is proposed using commercial 3D mesh data and 2D polygons that are rapidly and automatically produced through aerial LiDAR (Light Detection and Ranging) or RGB (Red Green Blue) cameras. During the data construction process, the original 3D mesh data was geometrically transformed so that each object could be expressed in various CityGML LoD (Levels of Detail), and attribute information extracted from the 2D spatial information data was used as a supplement to increase the utilization as spatial information. The 3D city features produced in this study are CityGML building, bridge, cityFurniture, road, and tunnel. Data conversion for each feature and property construction method were presented, and visualization and validation were conducted.

• Journal of Cadastre & Land InformatiX
• /
• v.54 no.1
• /
• pp.89-102
• /
• 2024

The recent advancements in Smart City and Digital Twin technologies have highlighted the critical role of integrating GIS and BIM in urban planning and construction projects. This integration ensures the consistency and accuracy of information, facilitating smooth information exchange. However, achieving interoperability requires standardization and effective project integration management strategies. This study proposes interoperability solutions for the integration of GIS and BIM for managing various projects. The research involves an in-depth analysis of the IFC schema and data structures based on the latest IFC4 version and proposes methods to ensure the consistency of reference point coordinates and coordinate systems. The study was conducted by setting the EPSG:5186 coordinate system, used by the National Geographic Information Institute's digital topographic map, and applying virtual shift origin coordinates. Through BIMvision, the results of the shape and error check coordinates' movement in the BIM model were reviewed, confirming that the error check coordinates moved consistently with the reference point coordinates. Additionally, it was verified that even when the coordinate system was changed to EPSG:5179 used by Naver Map and road name addresses, or EPSG:5181 used by Kakao Map, the BIM model's shape and coordinates remained consistently unchanged. Notably, by inputting the EPSG code information into the IFC file, the potential for coordinate system interoperability between projects was confirmed. Therefore, this study presents an integrated and systematic management approach for information sharing, automation processes, enhanced collaboration, and sustainable development of GIS and BIM. This is expected to improve compatibility across various software platforms, enhancing information consistency and efficiency across multiple projects.

• Tuberculosis and Respiratory Diseases
• /
• v.51 no.2
• /
• pp.97-107
• /
• 2001

Background : Tuberculosis itself causes not only lung parenchymal destruction but also pulmonary vascular damage. Secondary emphysema also causes pulmonary vascular damage, which can develop as a late sequela of pulmonary tuberculosis. Therefore, pulmonary circulatory impairment tends to be more severe in post-tuberculosis emphysema than in primary emphysema. In post-tuberculosis emphysema, the right ventricular function may play an important role. However, little information regarding the right ventricular function is available. The purpose of this study was to evaluate and compare the right ventricular function between post-tuberculosis emphysema and primary emphysema. Method: Post-tuberculosis emphysema(PTE) or primary emphysema(PE) was diagnosed by history, HRCT finding and pulmonary function. Twenty patients with post-tuberculosis emphysema were matched with 20 patients with primary emphysema according to both $FEV-1$ and FVC. Arterial blood gas analysis and echocardiography were done at rest and immediately after symptom-limited exercise. The right ventricular function was evaluated with the right ventricular ejection fraction using a modification of Simpson's method. Results : There was no significant difference in the demographics and pulmonary function between the two groups. In post-tuberculosis emphysema, the $PaCO_2$ was higher ($42.9{\pm}4.7$ vs $38.8{\pm}3.1\;mmHg$ at rest; $47.9{\pm}7.0$ vs $41.1{\pm}5.9\;mmHg$ after exercise; p<0.01) and the right ventricular ejection fraction was lower ($57.6{\pm}6.5$ vs $61.4{\pm}4.7%$ at rest; $51.1{\pm}10.8$ vs $59.8{\pm}6.6%$ after exercise; p<0.01) both at rest and after exercise. The $PaCO_2$ after exercise was also lower ($65.7{\pm}12.6$ vs $80.2{\pm}14.4\;mmHg$, p<0.01), while the Pa02 at rest tended to be lower($82.9{\pm}12.0$ vs $87.8{\pm}7.5$, p>0.05). In both groups, right ventricular ejection fraction correlated with the $PaCO_2$ after exercise(PTE r=0.536, PE r=0.557), and the $PaCO_2$ at rest(PTE r=-0.576, PE r=-0.588) and after exercise(PTE r=-0.764, PE r=-0.619). Conclusion : Impairment of the right heart function and gas exchange were more serious in post-tuberculosis emphysema than in primary emphysema, and gas exchange may be influenced by the right ventricular function in post-tuberculosis emphysema.