• Parasites, Hosts and Diseases
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• v.26 no.2
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• pp.73-86
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• 1988

The sensitivity and specificity of crude and affinity-purified antigens of Clcnorchis sinensis obtained from the infected rabbits were studied. Stage-specific antigenic proteins from the eggs, metacercariae and adult worms were characterized by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and enzyme-linked immunosorbent astray (ELISA). The results were as follows: 1. The antibody.binding antigen (ABA) purified from whole worm crude antigen (IVWA) by CNBr-activated Sepharose 4B affinity chromatography made :l specific bands against rabbit antisera on Ouchterlony gel diffusion plate, while WWA made 7 bands. Major WWA protein bands by SDS-PAGE were found at 16, 300~18, 500 and 28, 000~29, 000 daltons, while major ABA protein bands were at 18, 000~21, 000 and 29, 000~31, 000 daltons. The reactivity of ABA with rabbit anti-sera in ELISA was remarkably less sensitive than that of WWA. 2. Molecular weights of egg antigen (EGA), metacercarial antigen (MEA) and adult worm antigen (WWA) of C. sinensis ranged from 15, 000-200, 000 daltons, 15, 000-100, 000 daltons and 11, 000~80, 000 daltons, respectively. Major WWA proteins consisted mainly of polypeptide bands of low molecular weight, less than 31, 000 daltons, while those of EGA and MEA consisted of higher molecular T.eights than 30, 000 daltons. 3. The ELISA reactivities of WWA to rabbit anti.sera were remarkably greater than those of MEA. EGA showed negative reaction throughout the experiments. WWA showed higher optical density (O.D.) than 1.0, when reacted with rabbit anti-sera obtained at 4~6 weeks after the infection. In the rabbit anti-sera later than 12 weeks after the infection, the O.D. reacting witll WWA showed a plateau without variation. MEA shoT.ed relatively low O.D. values (<0.6), when reacted with anti-sera from lightly in(ected groups throughout the experiments, althougll there were some wealth positive cases (O.D.>0.6) ill heavily infected groups. MEA reacted with rabbit anti-sera showed negative results on Ouchterlony gel diffusion plates. Summarizing the above results, it is suggested that the whole worm antigen prepared from the adult worms of C. sinensis is most highly antigenic. However, this antigen might reveal cross reactions with other trematodes such as Paragonimus westermani, therefore, purification of antigenic proteins from the crude antigen is essential 18 increase the sensitivity and specificity for the immuncdiagnosis of clonorchiasis.

• Feed Journal
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• v.4 no.12 s.40
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• pp.136-141
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• 2006

소에서 발생하는 호흡기 질병은 가축이 가지고 있는 내적인 요인과 사육환경의 악화나 각종 스트레스 등의 외적인 요인과의 복합적인 균형의 파괴에서 시작된다고 볼 수 있다. 이와 같은 질병발생요인은 다른 축종이나 소화기 등 타 질병에서도 동인한 사항으로 생각될 수 있으나 호흡기질병의 경우 환경적인 요인이 더욱 중요한 발병이자로 작용하게 된다. 즉 밀집다두사육, 환절기의 낮과 밤의 큰 일교차, 환기불량이나 우사내의 유해가스, 장거리 수송 등의 스트레스가 일차적인 발병인자가 되며, 이타적으로 가축이 가지고 있거나 외부에서 유입된 여러 가지의 병원 미생물의 다량증식하고, 동물개체는 면역력이 떨어지면서 질병에 감염되어 단일 또는 복합적인 임상증상으로 나타나는 것이다. 호흡기질병의 원인균에는 다양한 종류의 세균 및 바이러스에 의해 이루어지며, 단일의 원인균에 의한 질병발생보다는 복합적으로 감염되어 병을 악화시키고 피해를 크게 하는 것이 일반적이다. 본고에서는 소에서 흔히 나타날 수 있는 주요한 세균성 호흡기 질병의 종류와 임상증상 및 일반적인 방제대책에 대하여 기술하고자 한다.

• Journal of Korean Dental Hygiene Science
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• v.6 no.1
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• pp.27-36
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• 2023

Background: The purpose of the study was to investigate the level of infection control and prevention awareness among dental practitioners in Korea based on the infection control and prevention guidelines of the Centers for Disease Control and Prevention (CDC) in the United States. Methods: A survey was created on 'Standard and Education on CDC Infection Control Standards', 'Matters related to CDC Infection Control Prevention', and 'Characteristics of Research Subjects and Infection Control Characteristics' based on the CDC Dental Infection Control List.' A total of 222 surveys were conducted and used for frequency and cross-tabulation analyses. Results: Most research participants worked at university or general hospitals, and 93.7% had received infection prevention education within the past year. The average awareness of the CDC dental infection control standards and education was 77.2 %, which was higher than previous research results. Preventive awareness was 71.5% on average, and there was a statistically significant difference in preventive awareness between the certified and non-certified evaluation groups (p<0.001). Conclusion: The participants of this study showed a higher awareness of infection control standards, education, and prevention than those in previous studies. However, this was insufficient compared with the CDC dental infection control standard prevention guidelines. Therefore, government agencies and related organizations must establish systematic infection control systems.

• Journal of fish pathology
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• v.10 no.2
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• pp.75-86
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• 1997

Solid phase enzyme linked immunosorbent assay (ELISA) was developed to detect the whole cells of Edwardsiella tarda from infected tissues of flounder. Cross-reaction test was performed by ELISA against fish pathogens such as A. hydrophila ATCC7966. V. anguillarum HYUFP5001, Y, ruckeri 11-4, E. ictaluri and Streptococcus sp. NG8206. Rabbit anti-E, tarda Edk-2 sera highly cross-reacted with A. hydrophila ATCC7966 and V. anguillarum HUFP5001. However, the cross-reaction was removed by using the anti-serum pre-adsorbed with A, hydrophila ATCC7966 FKC. The intra-species cross-reaction among E. tarda isolates was very high. ELISA with the whole cell antigens present in tissue homogenate appeared with highly decreased sensitivity, presumably by the co-coating of lipid or proteins in tissues. Thus, it would be necessary to use the infected tissue homogenates diluted more than 100 times with PBS for diagnosis. Interestingly, compared with the using of FKC antigen, the direct detection of viable cells in tissue homogenate showed more sensitive results with detection limit of $1{\times}10^3$ cells/ml in buffer or diluted tissue homogenate. Consequently, the ELISA method developed in this study was specific, rapid and sensitive for diagnosing edwardsiellosis.

• Journal of radiological science and technology
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• v.27 no.3
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• pp.51-58
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• 2004

The gel, which is stained on probe after ultrasonography, is a good circumstances for proliferation of microbe. This study is to investigate into the actual state of sanitary management, recognition degree and infection level of ultrasonographic probes. We had performed a question with telephone to 42 hospitals in Seoul area from December in 2003. We also cultured to obtained a sample from three ultrasonographic units to investigate infection level of the probes. Sanitary management of the probes was performed in 21 hospitals with alcohol cotton. Sanitary management was performed daily in 14 hospitals. Most hospitals used cotton towel for clearing of gel stained on probes. Preventive management against infection was performed in 32 hospitals with vinyl cover, surgical glove, or alcohol sterilization etc. In the recognition degree on infection, the response that using method of ultrasonographic probes is insanitary were in 78.6%(33 hospitals), and 54.8%(23 hospitals) responded that bacteria can be infected through the probes. In the results of germiculture, bacteria and fungi were detected too number of to count, but escherichia coli was not detected. In conclusion, The gel stained on probe after ultrasonography must be cleared completely, and it is necessary that change of recognition on sanitary management.

• Parasites, Hosts and Diseases
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• v.36 no.1
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• pp.37-46
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• 1998

A Czonorchis sinensis-specific antigen in excretory-secretory product of C. sinensis (CsE) was assessed in human clonorchiasis by immunoblot. Thirty and 7 kDa antigens of CsE2, one of four different batches of CsEs reacted strongly with infection sera from clonorchiasis patients; however, the antigens reacted weakly with 6-month post- treatment sera from praziquantel-cured cases, but were still highly detected by the sera from praziquantel∼failed patients, indicating that the 30 and 7 kDa antigens can detect antibodies during an active infection. The 30 kDa antigen showed some cross reactions with sera from patients with Pcragonimus westemani and Metcfonimw vokogcujci, while the 7 kDa antigen did not, suggesting that the 7 kDa antigen has high specificity. The 30 kDa antigen reacted with some past clonorchiasis sera, whereas the 7 kDa antigen did not, supporting that antibodies to the 7 kDa antigen are not present in sera from past clonorchiasis patients. In an endemic area, 92% (23/25) of active clonorchiasis patients and 91% (10/11) of mixed infection patients with C. sinensis and M. Wokosawai had IgG antibodies to the 7 kDa antigen, while 40% (6/15) of past clonorchiasis individuals and 43% (3/7) of metagonimiasis patients cross-reacted to the antigen. These data suggest that the 7 kDa antigen in an excretory-secretory antigen may serve as a marker of an active clonorchiasis with reliable specificities in past clonorchiasis, paragonimiasis and metagonimiasis.

• Journal of Korean society of Dental Hygiene
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• v.16 no.4
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• pp.517-523
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• 2016

Objectives: The purpose of the study is to investigate the uniform management of the dental hygienists in Korea. Methods: The study was carried out by direct telephone survey to dental gygienists in Gangneung, Wonju, and Seongnam from March 15 to April 10, 2015. The subjects were seventy dental clinics by random sampling. The questionnaire consisted of general characteristics of the subjects(4 items), infection control education(3 items), and infection control activity(9 items). Cronbach's alpha was 0.781 in the study. After informed consent from the dental hygienists, the survey was done by telephone. Data were analyzed by SPSS 21.0 program. Results: The dental hygienists taking infection control education tended to wash the uniforms twice a week (${\chi}^2$=15.95, p<001). The majority of the dental hygienists thought that the best washing place of uniform would be dental clinics than any other place (${\chi}^2$=38.76, p<001). Conclusions: It is very important to implement the infection control education for the dental hygienists. The establishment of standard guideline for infection control of the uniform washing will enhance the dental service quality and satisfaction of the dental service consumers.

• The Journal of Korean Academic Society of Nursing Education
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• v.23 no.4
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• pp.378-388
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• 2017

Purpose: The aim of this study was to identify the status of performance on standard precautions among nursing students and to examine the mediating effects of internal health locus of control on the relationship between awareness and performance on standard precautions of hospital-acquired infection control. Methods: The participants in this study were 134 nursing students. The measurements included a standard precautions awareness and performance scale, and a multidimensional health locus of control scale. Data were analyzed using independent t-test, one-way ANOVA, $Scheff{\acute{e}}$ test, Pearson correlation coefficient, and simple and multiple regression techniques. Mediation analysis was performed by the Baron and Kenny's method and Sobel test. Results: The mean score of standard awareness, standard performance, and internal health locus of control about standard precaution were $174.30{\pm}9.08$; $169.48{\pm}12.04$; and $20.43{\pm}2.82$; respectively. There was a positive correlation between standard awareness and performance (r=.414, p<.001). Also, standard awareness was significantly correlated with internal health locus of control (r=.413, p=.014). Internal health locus of control showed partial mediating effects in the relationship between awareness and performance. Conclusion: The results indicate a need to improve the internal health locus of control of nursing students. Therefore, an internal health locus of control improvement program should improve performance on standard precautions for patients and themselves.

• Journal of Korean Biological Nursing Science
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• v.20 no.1
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• pp.38-46
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• 2018

Purpose: The aim of this study was to verify the effects of daily 2% chlorhexidine gluconate (CHG) bathing on the acquisition of multidrug-resistant organisms (MDRO) and healthcare-associated infection (HAI) in a medical intensive care unit (MICU). Methods: The study was a randomized controlled group posttest only design, involving 91 patients in MICU at a tertiary hospital (47 patients in the experimental group and 44 patients in the control group). The 2% CHG bathing was performed daily according to bathing protocol to the patients in the experimental group, and traditional bath was performed every three days to those in the control group. Fisher's exact test and x2 test were used to analyze the data. Results: MDRO were found in 6 patients of the experimental group and in 15 patients of the control group. The difference was statistically significant (p= .016). HAI occurred in 2 patients of the experimental group and in 7 patients of the control group. The difference was not statistically significant (p= .084). Conclusion: The results confirmed that daily bathing with CHG was effective in reducing the incidence of MDRO acquisition. Therefore, it is expected that daily bathing with CHG will be used as an effective nursing intervention to reduce the incidence of MDRO acquisition.

• Research in Plant Disease
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• v.7 no.3
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• pp.155-163
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• 2001

Two attenuated Cucumber mosaic virus (CMV) isolates, Paf-CMV and Rs2-CMV that had been selected from CMV isolates associated with satellite RNA (satRNA) were tested for cross-protection effect in pepper plants. The viruses selected as attenuated strains appeared to be identical serologically and physically to the challenge virus (Mf-CMV), but they were lower in the dilution end-point of infectivity of crude sap than Mf-CMV When symptoms were observed in several indicator plants after inoculation, Paf-CMV and Rs2-CMV were symptomless or showed mild mosaic symptoms while another satRNA isolate Ap-CMV developed severe mosaic symptoms on the leaves as Mf-CMV. The nucleotide sequences of the satRNAs were determined by sequencing full-length cDNA clones. Paf-, Rs2- and Ap-satRNAs were 386, 335, and 347 nucleotides long, respectively, The sequences were then compared with the other known Y-satRNA, revealing that nucleotide sequences of the satRNAs consisted of 5'- and 3'-terminal conserved regions. However variations occurred on the middle regions of the sequences, especially those related to symptom interference, showing significant differences between Paf-satRNA and other isolates. Infectious transcripts of Paf-satRNA and Rs2-satRNA induced mild mosaic symptoms in pepper plants when supported by genomic RNAs of Mf-CMV. Under greenhouse conditions, Paf-CMV and Rs2-CMV were tested for cross-protection effect in pepper and tobacco (Nicotiana tabacum cv, Xanthi nc) plants against Mf-CMV. No symptoms were developed on the plants vaccinated with Paf-CMV until 3 weeks after inoculation with the virulent strain; however another attenuated isolate, Rs2-CMV, showed less effectiveness in cross-protection. Depending on the concentration of the challenged virus, symptoms sometimes appeared later in the upper leaves. However, in plants challenged with low concentrations (below 0.2 mg/ml) of the challenge inoculum, symptoms caused by the virulent strain did not develop on the plants vaccinated with Paf-CMV. In the field experiments, the number of pepper plants with severe mosaic symptoms in the control plots was progressively increased after transplanting and reached approximately 50% after 50 days. On the other hand, the incidence of mosaic disease appeared very low on the plants that had received the protective inoculation with Paf-CMV.