• Korean Journal of Optics and Photonics
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• v.23 no.3
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• pp.108-112
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• 2012

In this paper, two wavelengths mainly used for laser prostatectomy have been quantitatively compared: 532 and 980 nm. Two lasers at 40 W were employed to ablate bovine liver in vitro. Ablation performance was evaluated in light of number of sweeps, ablation volume, and coagulative necrosis. 532 nm yielded up to four times higher ablation efficiency than 980 nm. Regardless of wavelength, ablation rate per sweep decreased with the number of sweeps. 532 nm generated relatively deeper ablation craters along with thinner coagulation whereas 980 nm created superficial tissue ablation with up to 2 mm thick coagulative necrosis. Due to higher light absorption and effective thermal confinement, 532 nm induced more efficient tissue ablation with a smaller coagulative necrotic zone. The current study demonstrated that 532 nm could be a more ideal wavelength for laser prostatectomy, and the future in vivo investigations will confirm these findings.

• Journal of the Korean Society of Radiology
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• v.6 no.3
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• pp.207-215
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• 2012

As one of photo dynamic therapies, the existing LED photo irradiation method with 635 nm continuous wave has most frequently been used for acne treatment, it suffered from a low energy efficiency and generation of a large amount of heat in tissues requiring improvement measures. In this thesis, a LED photo irradiation system for acne treatment has been designed using PWM(Pulse Width Modulation) mode to enhance the energy efficiency and prevent thermal destruction in tissues. System configuration consisting largely of timer module, PWM module, and photo transfer device has been designed with the use of 1 W LED at a wavelength of 660 nm for the photo transfer device to increase skin penetration depth for treatment of acne. Frequency and wave form generated by using PWM control was verified along with confirmation of output energy of 660 nm LED and surface temperatures of tissues, followed by evaluation of stable energy outputs and stability of tissues. The results indicated that whereas power loss was high and thermal destruction in tissues was exhibited when C.W mode was used to obtain the optical energy of 1 W LED at a wavelength of 660 nm for acne treatment, realization of PWM mode allowed lowering of power consumption for LED through pulse width modulation, and no occurrence of thermal destruction in tissues, suggesting that PWM mode is safer and more effective for treatment of acne than C.W mode.

• Applied Chemistry for Engineering
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• v.28 no.4
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• pp.383-396
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• 2017

The photothermal therapy is a method of cell ablation using the heat converted from the incident light by photothermal transducers. It offers a selective treatment to desired abnormal cells, in particular, tumor tissues. Among various photothermal agents, gold nanoparticles (Au NPs) have received enormous attention due to their unique physicochemical property over last two decades. In this review, we address research strategies and methods to improve treatment efficacy by organizing recent research works. We mainly focus on research works to enhance light-to-heat conversion via optimizing the morphology of Au NPs and related assemblies as well as the strategies to deliver Au NPs efficiently to specific targets. We also introduce convergence research efforts to combine Au NP-mediated photothermal treatment and other functions such as diagnostic capabilities and other therapeutic methods.

• Nuclear Medicine and Molecular Imaging
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• v.42 no.5
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• pp.383-393
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• 2008

Purpose: Cancer specific killing can be achieved by therapeutic gene activated by cancer specific promotor. Expression of sodium iodide symporter (NIS) gene causes transportation and concentration of iodide into the cell, therefore radioiodine treatment after NIS gene transfer to cancer cell could be a form of radionuclide gene therapy. luciferase (Luc) gene transfected cancer cell can be monitored by in vivo optical imaging after D-luciferin injection. Aims of the study are to make vector with both therapeutic NIS gene driven by AFP promoter and reporter Luc gene driven by CMV promoter, to perform hepatocellular carcinoma specific radiodiodine gene therapy by the vector, and assessment of the therapy effect by optical imaging using luciferase expression. Materials and Methods: A Vector with AFP promoter driven NIS gene and CMV promoter driven Luc gene (AFP-NIS-CMV-Luc) was constructed. Liver cancer cell (HepG2, Huh-7) and non liver cancer cell (HCT-15) were transfected with the vector using liposome. Expression of the NIS gene at mRNA level was elucidated by RT-PCR. Radioiodide uptake, perchlorate blockade, and washout tests were performed and bioluminescence also measured by luminometer in these cells. In vitro clonogenic assay with 1-131 was performed. In vivo nuclear imaging was obtained with gamma camera after 1-131 intraperitoneal injection. Results: A Vector with AFP-NIS-CMV-Luc was constructed and successfully transfected into HepG2, Huh-7 and HCT-15 cells. HepG2 and Huh-7 cells with AFP-NIS-CMV-Luc gene showed higher iodide uptake than non transfected cells and the higher iodide uptake was totally blocked by addition of perchlorate. HCT-15 cell did not showed any change of iodide uptake by the gene transfection. Transfected cells had higher light output than control cells. In vitro clonogenic assay, transfected HepG2 and Huh-7 cells showed lower colony count than non transfected HepG2 and Huh-7 cells, but transfected HCT-15 cell did not showed any difference than non transfected HCT-15 cell. Number of Huh-7 cells with AFP-NIS-CMV-Luc gene transfection was positively correlated with radioidine accumulation and luciferase activity. In vivo nuclear imaging with 1-131 was successful in AFP-NIS-CMV-Luc gene transfected Huh-7 cell xenograft on nude mouse. Conclusion: A Vector with AFP promoter driven NIS and CMV promoter driven Luc gene was constructed. Transfection of the vector showed liver cancer cell specific enhancement of 1-131 cytotoxicity by AFP promoter, and the effect of the radioiodine therapy can be successfully assessed by non-invasive luminescence measurement.

• Korean Journal of Optics and Photonics
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• v.28 no.5
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• pp.236-240
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• 2017

In this paper, we investigate the thermal response of skin tissue to high-intensity focused ultrasound (HIFU) by means of infrared (IR) thermal imaging. For skin tightening, a 7-MHz ultrasound transducer is used to induce irreversible tissue coagulation in porcine skin. An IR camera is employed to monitor spatiotemporal changes of the temperature in the tissue. The maximum temperature in the tissue increased linearly with applied energy, up to $90^{\circ}C$. The extent of irreversible tissue coagulation (up to 3.2 mm in width) corresponds well to the spatial distribution of the temperature during HIFU sonication. Histological analysis confirms that the temperature beyond the coagulation threshold (${\sim}65^{\circ}C$) delineates the margin of collagen denaturation in the tissue. IR thermal imaging can be a feasible method for quantifying the degree of thermal coagulation in HIFU-induced skin treatment.

• Journal of Dental Rehabilitation and Applied Science
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• v.40 no.2
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• pp.55-63
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• 2024

Purpose: This study aimed to assess the antimicrobial efficacy of an 810-nm infrared diode laser with indocyanine green (ICG) against Staphylococcus aureus on sandblasted, large grit, and acid-etched (SLA) titanium surfaces, comparing its effectiveness with alternative chemical decontamination modalities. Materials and Methods: Biofilms of S. aureus ATCC 25923 were cultured on SLA titanium disks for 48 hours. The biofilms were divided into five treatment groups: control, chlorhexidine gluconate (CHX), tetracycline (TC), ICG, and 810-nm infrared diode laser with ICG (ICG-PDT). After treatment, colony-forming units were quantified to assess surviving bacteria, and viability was confirmed through confocal laser-scanning microscope (CLSM) imaging. Results: All treated groups exhibited a statistically significant reduction in S. aureus (P < 0.05), with notable efficacy in the CHX, TC, and ICG-PDT groups (P < 0.01). While no statistical difference was observed between TC and CHX, the ICG-PDT group demonstrated superior bacterial reduction. CLSM images revealed a higher proportion of dead bacteria stained in red within the ICG-PDT groups. Conclusion: Within the limitations, ICG-PDT effectively reduced S. aureus biofilms on SLA titanium surfaces. Further investigations into alternative decontamination methods and the clinical impact of ICG-PDT on peri-implant diseases are warranted.

• Radiation Oncology Journal
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• v.12 no.1
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• pp.27-31
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• 1994

There are a number of reports suggesting that there may be a correlation between the clinical response to radiotherapy in various tumors and the clonogenic survival of cell lines derived from these tumors following exposure to 2 Gy(SF2). Authors conducted this study to determine SF2 for cells in primary culture from surgical specimens. The tumor tissues with squamous cell carcinoma of uterine cervix and head and neck were obtained. The tumor tissues were disaggregated to single cells by incubating with collagenase type w for 2 hours with constant stirring. Single cell suspensions were inoculated in four 24-well plates precoated with cell adhesive matrix. After 24 hours of incubation at 37$ ^{\circ}C $, rows of four wells were then irradiated, consisting of control set and five other sets each receiving doses of 1,2,3,4, and 6 Gy. After incubation for a total of 13 days, the cultures were stained with crystal violet and survival at each dose was determined by quantitative image analysis system, To determine whether cell growth was of epithelial origin, immunocytochemical staining with a mixture of cytokeratin and epithelial monoclonal antibodies were performed on cell cultures. During the period of this study, we received 5 squamous cell carcinoma specimens of head and neck and 20 of uterine cervical carcinoma. Of these, 15 yielded enough cells for radiosensitivity testing. This resulted an overall success rate of 60$ \% $. The mean SF2 value for 15 tumours was 0.55$\pm$0.17 ranging from 0.20 to 0.79. These results indicate that there is a broad range of sensitivities to radiation in same histologic type. So with a large patient population, we plan to determine whether a different SF2 value is associated with tumours that are controlled with radiotherapy than those that are not.

• Proceedings of the KOR-BRONCHOESO Conference
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• 1982.05a
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• pp.13.2-14
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• 1982

Otitis media with effusion, described first by Politzer (1867), is closely related with the function of auditory tube, but its etiology and pathogenesis are not clearly defined yet. There are many theories about its pathogenesis including hydrops ex vacuo theory which was most reliable nowadays. In this paper, using cats in experimental animals, hydrops ex vacuo theory was proved and cytological study of the effusion and light microscopic observation of the middle ear mucosa in otitis media with effusion were done. The results were as follows: 1) The effusion was found in all experimental groups after eighteen hours of the auditory tube obstruction. 2) In the cytological study of effusion by smear technic, Polymorpholeukocytes were dominant in earlier days but monoculear cells were soon increased and no eosinophils were found. 3) In the culture of the effusion, no bacteria was cultured. 4) By opeating microscope, hypertrophy of the middle ear mucosa observed especially in the fourteen days after auditory tube obstruction and effusion was most remarkable in the fourteen days, also. 5) By light microscopy, there were epithelial hyperplasia, proliferation of goblet cells, capillaries and infiltration of inflammatory cells which showed same distribution as smear technic.