• Korean Journal of Plant Taxonomy
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• v.43 no.4
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• pp.312-318
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• 2013

Morphological characters of aggregated achenes, and achene and its epidermal microstructures were observed for 7 taxa belonging to the section Adonanthe of subgenus Adonanthe [A. amurensis Regel et Radde, A. bobroviana Sim., A. davidii Franch., A. multiflora Nishikawa et Ko. Ito, A. pseudoamurensis W. T. Wang, A. ramosa Franch. and A. tianschanica (Adolf) Lipsch. ex Bobrov] and for 1 taxon belonging to the section Lophocarpa of subgenus Adonis (A. aestivalis var. parviflora M. Bieb.). The morphological characters such as shape of aggregated achenes, length of aggregated achenes, shape of achene, and recurved type of persistent style were useful in the classification of the subgenera Adonanthe and Adonis, and each species of the series under section Adonanthe. Epidermal microstructures of achene were grouped into three types according to the presence of microwrinkles and their arranged patterns. These microwrinkles were absent in A. amurensis, were of parallel type in A. bobroviana, A. pseudoamurensis, A. ramosa, and A. aestivalis var. parviflora, and were of parallelperpendicular mixed type in A. davidii, A. multiflora, and A. tianschanica. Also, a new key to them was made using morphological characters of their aggregated achenes and achene.

• Korean Journal of Plant Tissue Culture
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• v.22 no.6
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• pp.351-355
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• 1995

A truncated Polygalacturonase (PG) cDNA was fused in reverse orientation to the CaMV 35S promoter of the binary vector pCA643, and introduced into tomato cells by Agrobaderium - mediated transformation. Transformed cells were selected using kanamycin as select agent then regenerated into plants. After selfed, one transgenic line (T9), was germinated and grown on MS medium containing 1 mg/mL of kanamycin Genomic Southern analysis of a T9 progeny with labelled PG2 cDNA probe showed a single antisense PC fragment as well as the endogenous PG2 gene, suggesting that PC antisense gene was integrated into tomato genome. Northern blot analysis demonstrated that the antisense RNA was produced from the transgene at much tiger level than the endogenous PG2 gene. Polygalacturonase activity analysis of the fruit from transgenic plants demonstrated that the antisense transgene expression caused 4 to 60% reduction of endogenous PG activity.

• Journal of Life Science
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• v.20 no.2
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• pp.292-297
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• 2010

Anthocyanin, a phenolic compound, is a pigment that shows blue or red color in the fruit, petal and other tissues. It is an important factor in grape berry skin pigment and accumulates only in the skin. This skin-specific accumulation of anthocyanin has been reported to be regulated by the ufgt gene which encodes UDP-glucose: flavonoid 3-O-glucosyltransferase that participates in the biosynthesis of anthocyanin. The ufgt gene is expressed only in berry skin, while the other genes involved in the biosynthetic pathway are expressed in both skin and flesh tissues. In order to determine whether anthocyanin accumulation is primarily regulated by compartment of UFGT, a ufgt cDNA clone was isolated from grape berry, its open reading frame was ligated in pBI121 vector in either a sense or an antisense orientation under the control of the CaMV35S promoter and the recombinant constructs were incorporated into tobacco plants. Several transgenic lines were selected and characterized to determine the level of expression of the grapevine ufgt transcript and endogenous homologs of tobacco. Compared to the wild-type, the amount of anthocyanins in sense transgenic plants increased by 44%, while the amount of anthocyanins in antisense transgenic plants decreased by 88%. In addition, the color of flowers became intense in the sense transgenic plants. These results suggest that over-expression or repression of the ufgt gene affected the accumulation of anthocyanin in flowers of tobacco.

• Korean Journal of Plant Taxonomy
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• v.40 no.1
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• pp.50-58
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• 2010

The nutlet morphology and pericarp structure of 14 taxa (4 taxa of the genus Glechoma and related taxa) were studied by light and scanning electron microscopy, and a detailed description of nutlet morphological features for all examined taxa is provided. The shape of the nutlet is ovate to elliptic. Its size is 1.36-3.83 mm in length and 0.80-2.65 mm in width. The largest one was found in Marmoritis rotundifolia, while the smallest one was in Agastache nepetoides. Three different surface types were recognized in the studied taxa based on differences in cell outline and cell boundary relief (rectangular, pitted, and ridged negative reticulate-cell). Three types of trichome (uni-cellular non-glandular trichome, multi-cellular non-glandular trichome, and peltate glandular trichome) were distributed in the nutlets. The pericarp is formed in three layers (i.e., exocarp, mesocarp, and endocarp including sclenchyma tissue), and the pericarp in the studied species is $39.0-237.5{\mu}m$ thick. The morphological and anatomical features (the nutlet shape, surface type, and trichome distribution) in the genus Glechoma and related taxa are described and discussed.

• Korean Journal of Plant Taxonomy
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• v.36 no.4
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• pp.309-333
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• 2006

Examined were the vegetative and reproductive characters such as external morphology, anatomy (leaf petiole, blade and stem), pollen and nutlet for the taxonomic revision of Elsholtzia in Korea. The inflorescence of all studied taxa of Elsholtzia in Korea is secund and 0.5-10 cm long. Leaves are ovate or nearly and $0.2-10{\times}0.2-6.0cm$. The shapes of inflorescence and leaves are very useful taxonomic characters. The stomata types are anomocytic and diacytic. The morphology of nutlet is ovoid and pitted in surface pattern of exocarp. All studied taxa are myxocarpy. The pollen grains are of medium size and subprolate in shape, and have typical bi-reticulate exine surface. Four species are recognized for Korean Elsholtzia by the identification key and descriptions in this study.

• Korean Journal of Plant Resources
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• v.9 no.3
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• pp.298-304
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• 1996

The fruit wall anatomy of Ocotea was investigated on the basis of 14 species within the genus to contribute to a better understanding of specific relationships and homogeneity of genus. The species have a similar mature fruit wall structure, but diUerences among the species are found with respect to whether or not sdc.nchyma cells are present in the mesocarp. if present, whether or not they are present in particular positions and forms. Comparisons with species studied suggested that at least a few groups of species can be distinguished in Ocotea. They arc divided into five groups on the basis of anatomical structures. i.e., group 1) O. atrriensis, O. cujumari, O. helicterifolia, O. rubra and O. schomburgkiana; group 2) O. aeiphylla, O. javitensis, and O. sp. [Werff et ai. 12676]; group 3) O. tonduzii: group 4) O. foetens, O. quixos, and O. veraguensis; and group 5) O. floribunda and O. nitida. These various variations in Ocntea were also discussed to invite its respective systematic revisions. By the comparisons with species, on the other hand, it suggested that the specialized species are evolved from non-specialized species.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.65 no.4
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• pp.485-495
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• 2020

Melon (Cucumis melo L.), one of the most important fruit crop species, is cultivated worldwide. In this study, a total of 206 melon accessions conserved at the National Agrobiodiversity Center (NAC) in RDA were characterized for nine morphological characteristics according to the NAC descriptor list. In addition, to confirm the genetic composition of each melon accession, genetic profiling was performed using 20 SSR markers. Among the 206 melon accessions, 159 (77.2%) were collected from Asia. The color of fruit flesh and skin were mostly 'white' (56.0%) and 'green' (49%), respectively. Days to female flowering (FD) and maturity (MD) of the accessions ranged from 58 to 72 and 17 to 63, respectively. The fruit length and width of the accessions ranged from 6.0 to 29.3 and 3.6 to 17.2 cm, respectively. The sugar content (SU) ranged from 2.5% to 13.2% with an average of 7.0%. In correlation analysis, SU showed positive and negative correlations with MD and FD, respectively. The accessions were classified into four clusters by cluster analysis. From the results of genetic profiling using 20 SSR markers, three accessions (K189118, K100486, and K190292) were expected to be inbred lines among 206 melon accessions. These results could expand the knowledge of the melon germplasm, providing valuable material for the development of new melon varieties to suit consumer tastes.

• Journal of Plant Biotechnology
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• v.42 no.4
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• pp.342-349
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• 2015

Kiwifruit is a new fruit crop that was commercialized in the late 1970s. Recently, its cultivation and consumption have increased rapidly worldwide. Kiwifruit is a dioecious, deciduous, and climbing plant having fruit with hairs and various flesh colors and a variation in ploidy level; however, the industry consists of very simple cultivars or genotypes. The need for efficient cultivar improvement together with the evolutional and biological perspectives based on unique plant characteristics, have recently encouraged genome analysis and bioinformatics application. The draft genome sequence and chloroplast genome sequence of kiwifruit were released in 2013 and 2015, respectively; and gene annotation has been in progress. Recently, transcriptome analysis has shifted from previous ESTs analysis to the RNA-seq platform for intensive exploration of controlled genetic expression and gene discovery involved in fruit ascorbic acid biosynthesis, flesh coloration, maturation, and vine bacterial canker tolerance. For improving conventional breeding efficiency, molecular marker development and genetic linkage map construction have advanced from basic approaches using RFLP, RAPD, and AFLP to the development of NGS-based SSR and SNP markers linked to agronomically important traits and the construction of highly saturated linkage maps. However, genome and transcriptome studies have been limited in Korea. In the near future, kiwifruit genome and transcriptome studies are expected to translate to the practical application of molecular breeding.

• Korean Journal of Plant Resources
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• v.32 no.4
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• pp.303-311
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• 2019

Various colors of fruit skin and flesh are the most popular commercial criteria for peach classification. In order to breed new red-fleshed peach cultivar, many cross seedlings and generations should be maintained. Therefore it is necessary to develop early selection markers to screen seedlings with target traits to increase breeding efficiency. For the comparison of transcription profiles in peach cultivars differing in flesh color expression, two cDNA libraries were constructed. Differences in gene expression between red-fleshed peach cultivar, 'Josanghyeoldo' and white-fleshed peach cultivar, 'Mibaekdo' were analyzed by next-generation sequencing (NGS). Expressed sequence tag (EST) of clones from the two cultivars were selected for nucleotide sequence determination and homology searches. Putative single nucleotide polymorphisms (SNP) were screened from peach EST contigs by high resolution melting (HRM) analysis displayed specific difference between 8 red-fleshed peach cultivars and 24 white-fleshed peach cultivars. All 72 pairs of SNPs were discriminated and the HRM profiles of amplicons were established. In the study reported here, the development of SNP markers for distinguishing between red and white fleshed peach cultivars by HRM analysis offers the opportunity to use DNA markers. This SNP marker could be useful for peach marker assisted breeding and provide a good reference for relevant research on molecular mechanisms of color variation in peach cultivars.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.56 no.1
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• pp.8-13
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• 2011

This study was carried out to compare botanical characteristics, physicochemical properties related to table quality and feed values and develop a new variety among 12 developed waxy com hybrids. These materials were produced in 2008 and cultivated in 2009 at Com Breeding and Genetics Farm of Coll. of Agri. & Life Sci., Chungnam Nat'l Univ. Botanical characteristics of used waxy com hybrids showed large difference among hybrids; stem height, ear height and ear length. In physical properties, range of kernel sugar content among used hybrids appeared 16.1 to 13.0brix(%). CNU08H-15, CNU08H-35 and CNU08H-h39 among these were highly appeared as 15 above and pericarp thickness of Yeonongchal was very thick as $46.0{\mu}m$, while CNU08H-39 among hybrids showed the thinnest as $23.0{\mu}m$ compared to other hybrids. Protein content as a indicator of table quality was the highest in Ilmichal among hybrids, while that of CNU08H-39 was the lowest, while amylopectin content was the highest as 94.5%, From experimental results, Daehakchal Gold 1, CNU08H-h121 and CNU08H-39 including check Yeonyoungchal were highly evaluated as new hybrid in thinner pericarp, high amylopectin and chewiness.