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http://dx.doi.org/10.7732/kjpr.2019.32.4.303

Development of SNP Molecular Marker for Red-fleshed Color Identification of Peach Genetic Resources  

Kim, Se Hee (Fruit Research Division, National Institute of Horticultural & Herbal Science)
Nam, Eun Young (Fruit Research Division, National Institute of Horticultural & Herbal Science)
Cho, Kang Hee (Fruit Research Division, National Institute of Horticultural & Herbal Science)
Jun, Ji Hae (Fruit Research Division, National Institute of Horticultural & Herbal Science)
Chung, Kyeong Ho (Fruit Research Division, National Institute of Horticultural & Herbal Science)
Publication Information
Korean Journal of Plant Resources / v.32, no.4, 2019 , pp. 303-311 More about this Journal
Abstract
Various colors of fruit skin and flesh are the most popular commercial criteria for peach classification. In order to breed new red-fleshed peach cultivar, many cross seedlings and generations should be maintained. Therefore it is necessary to develop early selection markers to screen seedlings with target traits to increase breeding efficiency. For the comparison of transcription profiles in peach cultivars differing in flesh color expression, two cDNA libraries were constructed. Differences in gene expression between red-fleshed peach cultivar, 'Josanghyeoldo' and white-fleshed peach cultivar, 'Mibaekdo' were analyzed by next-generation sequencing (NGS). Expressed sequence tag (EST) of clones from the two cultivars were selected for nucleotide sequence determination and homology searches. Putative single nucleotide polymorphisms (SNP) were screened from peach EST contigs by high resolution melting (HRM) analysis displayed specific difference between 8 red-fleshed peach cultivars and 24 white-fleshed peach cultivars. All 72 pairs of SNPs were discriminated and the HRM profiles of amplicons were established. In the study reported here, the development of SNP markers for distinguishing between red and white fleshed peach cultivars by HRM analysis offers the opportunity to use DNA markers. This SNP marker could be useful for peach marker assisted breeding and provide a good reference for relevant research on molecular mechanisms of color variation in peach cultivars.
Keywords
Anthocyanin; Breeding; Cultivars; Gene expression; High resolution melting;
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